Regenera??o in vitro de Vellozia sincorana Ayensu & Smith
Ano de defesa: | 2015 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Estadual de Feira de Santana
|
Programa de Pós-Graduação: |
Mestrado Acad?mico em Recursos Gen?ticos Vegetais
|
Departamento: |
DEPARTAMENTO DE CI?NCIAS BIOL?GICAS
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://localhost:8080/tede/handle/tede/277 |
Resumo: | Vellozia sincorana Ayensu & SMITHis endemic of Rupestres Fields of Chapada Diamantina, popularly known as Candomb?. This species has a highly flammable resin, and the stem used to ignite wood stoves and roots to produce incense. Candomb? presents few studies in the literature, there are no studies of propagation of the species. This study aimed to develop in vitro multiplication protocol of V. sincorana, analyzing the behavior of in vitro shoots subjected to stress, and its influence on multiplication. They were used as explants in vitro shoots obtained, inoculated with concentrations of BAP (0.0; 2.22; 4.44 ?M), CIN (0.0; 2.32; 4.64 ?M) and IBA (0.0; 1.48 ?M) in MS ? medium supplemented with 87.64 mM sucrose, 1g.L-1activated carbon, and 0.7% agar (basic medium). Subsequently, it tested concentrations of cytokinins BAP or KIN (0.0; 5.0; 10.0; 15.0; 20.0 ?M). In experiments with thermal stress, flame shoots were exposed for different periods (0.0; 2.0; 4.0; 6.0; 8.0; 10 seconds), and the next tested whether the periods (0.0; 4.5; 6.0; 7.5; 9.0 min) exposure of the shoots 105 ? 1 ? C in sterilize. For drought stress basic medium was agar supplemented with different concentrations (7.0; 14.0; 21.0; 28.0 g) and the following experiment different sucrose concentrations (87.64; 175.28; 262. 92; 350.56 mM). The KIN showed greater efficacy in the multiplication in relation to BAP. The highest average for number of shoots and responsive% explants were in the periods 6, 8 or 10 seconds, being observed tolerance to water and thermal stress. |
id |
UEFS_c72541dd4f4eb746ae7b982dac9a17eb |
---|---|
oai_identifier_str |
oai:tede2.uefs.br:8080:tede/277 |
network_acronym_str |
UEFS |
network_name_str |
Biblioteca Digital de Teses e Dissertações da UEFS |
repository_id_str |
|
spelling |
Santana, Jos? Raniere Ferreira de55155448549http://lattes.cnpq.br/837165033057126103268282571http://lattes.cnpq.br/5248371924510498Borges, B?rbara Paula dos Santos2015-12-02T20:10:10Z2015-08-26BORGES, B?rbara Paula dos Santos. Regenera??o in vitro de Vellozia sincorana Ayensu & Smith. 2015. 70 f. Disserta??o (Mestrado Acad?mico em Recursos Gen?ticos Vegetais) - Universidade Estadual de Feira de Santana, Feira de Santana, 2015.http://localhost:8080/tede/handle/tede/277Vellozia sincorana Ayensu & SMITHis endemic of Rupestres Fields of Chapada Diamantina, popularly known as Candomb?. This species has a highly flammable resin, and the stem used to ignite wood stoves and roots to produce incense. Candomb? presents few studies in the literature, there are no studies of propagation of the species. This study aimed to develop in vitro multiplication protocol of V. sincorana, analyzing the behavior of in vitro shoots subjected to stress, and its influence on multiplication. They were used as explants in vitro shoots obtained, inoculated with concentrations of BAP (0.0; 2.22; 4.44 ?M), CIN (0.0; 2.32; 4.64 ?M) and IBA (0.0; 1.48 ?M) in MS ? medium supplemented with 87.64 mM sucrose, 1g.L-1activated carbon, and 0.7% agar (basic medium). Subsequently, it tested concentrations of cytokinins BAP or KIN (0.0; 5.0; 10.0; 15.0; 20.0 ?M). In experiments with thermal stress, flame shoots were exposed for different periods (0.0; 2.0; 4.0; 6.0; 8.0; 10 seconds), and the next tested whether the periods (0.0; 4.5; 6.0; 7.5; 9.0 min) exposure of the shoots 105 ? 1 ? C in sterilize. For drought stress basic medium was agar supplemented with different concentrations (7.0; 14.0; 21.0; 28.0 g) and the following experiment different sucrose concentrations (87.64; 175.28; 262. 92; 350.56 mM). The KIN showed greater efficacy in the multiplication in relation to BAP. The highest average for number of shoots and responsive% explants were in the periods 6, 8 or 10 seconds, being observed tolerance to water and thermal stress.Vellozia sincorana AYENSU & SMITH ? end?mica dos Campos Rupestres da Chapada Diamantina, conhecida popularmente como Candomb?. Essa esp?cie possui resina altamente inflam?vel, sendo o caule utilizado para acender fog?es a lenha e as ra?zes para produzir incenso. O Candomb? apresenta poucos trabalhos na literatura, n?o havendo estudos de propaga??o da esp?cie. Esse trabalho teve como objetivo desenvolver protocolo de multiplica??o in vitro de V. sincorana, analisando o comportamento dos brotos in vitro submetidos ao estresse, e sua influ?ncia na multiplica??o. Foram utilizados como explantes brotos obtidos in vitro, inoculados em concentra??es de BAP (0,0; 2,22; 4,44 ?M), CIN (0,0; 2,32; 4,64 ?M) e AIB (0,0; 1,48 ?M) em meio MS ?suplementado com 87,64mM de sacarose, 1g.L-1 de carv?o ativado, e0,7% de ?gar (meio b?sico).No experimento seguinte, testou-se concentra??es das citocininas BAP ou CIN (0,0; 5,0; 10,0; 15,0; 20,0 ?M). Nos experimentos com estresse t?rmico, os brotos foram expostos a flambagem por diferentes per?odos (0, 2, 4, 6, 8; 10 segundos), e no seguinte testou-se os per?odos (0,0, 4,5, 6,0, 7,5; 9,0 min.) de exposi??o dos brotos a 105?1?C, em estufa. Para estresse h?drico o meio b?sico foi suplementado com diferentes concentra??es de ?gar (7,0, 14,0, 21,0, 28,0 g) e no experimento seguinte diversas concentra??es de sacarose (87,64, 175,28, 262,92; 350,56 mM).O CIN apresentou maior efic?cia na multiplica??o, em rela??o ao BAP. As maiores m?dias para n?mero de brotos e % explantes responsivos foram nos per?odos 6, 8 e 10 segundos, sendo observada toler?ncia aos estresses h?drico e t?rmico.Submitted by Luis Ricardo Andrade da Silva (lrasilva@uefs.br) on 2015-12-02T20:10:10Z No. of bitstreams: 1 v.final DISSERTA??O B?RBARA PAULA DOS SANTOS BORGES DOS SANTOS BORGES.pdf: 1253496 bytes, checksum: b385afcd4779d54bd2a324ab4f1f5b5f (MD5)Made available in DSpace on 2015-12-02T20:10:10Z (GMT). No. of bitstreams: 1 v.final DISSERTA??O B?RBARA PAULA DOS SANTOS BORGES DOS SANTOS BORGES.pdf: 1253496 bytes, checksum: b385afcd4779d54bd2a324ab4f1f5b5f (MD5) Previous issue date: 2015-08-26Funda??o de Amparo ? Pesquisa do Estado da Bahia - FAPEBapplication/pdfporUniversidade Estadual de Feira de SantanaMestrado Acad?mico em Recursos Gen?ticos VegetaisUEFSBrasilDEPARTAMENTO DE CI?NCIAS BIOL?GICASCandomb?Micropropaga??oReguladores vegetaisEstresse h?dricoEstresse t?rmicoCandomb?MicropropagationGrowth regulatorsWater stressHeat stressBOTANICA::FISIOLOGIA VEGETALCIENCIAS BIOLOGICAS::GENETICARegenera??o in vitro de Vellozia sincorana Ayensu & Smithinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis2441563959449389152600600600600600-6971480722008537872-289993050269505198-55181442685852520514767858349021390776info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEFSinstname:Universidade Estadual de Feira de Santana (UEFS)instacron:UEFSORIGINALv.final DISSERTA??O B?RBARA PAULA DOS SANTOS BORGES DOS SANTOS BORGES.pdfv.final DISSERTA??O B?RBARA PAULA DOS SANTOS BORGES DOS SANTOS BORGES.pdfapplication/pdf1253496http://tede2.uefs.br:8080/bitstream/tede/277/2/v.final+DISSERTA%C3%87%C3%83O+B%C3%81RBARA+PAULA+DOS+SANTOS+BORGES+DOS+SANTOS+BORGES.pdfb385afcd4779d54bd2a324ab4f1f5b5fMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://tede2.uefs.br:8080/bitstream/tede/277/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/2772015-12-02 17:10:10.546oai:tede2.uefs.br:8080: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.uefs.br:8080/PUBhttp://tede2.uefs.br:8080/oai/requestbcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.bropendoar:2015-12-02T20:10:10Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS)false |
dc.title.por.fl_str_mv |
Regenera??o in vitro de Vellozia sincorana Ayensu & Smith |
title |
Regenera??o in vitro de Vellozia sincorana Ayensu & Smith |
spellingShingle |
Regenera??o in vitro de Vellozia sincorana Ayensu & Smith Borges, B?rbara Paula dos Santos Candomb? Micropropaga??o Reguladores vegetais Estresse h?drico Estresse t?rmico Candomb? Micropropagation Growth regulators Water stress Heat stress BOTANICA::FISIOLOGIA VEGETAL CIENCIAS BIOLOGICAS::GENETICA |
title_short |
Regenera??o in vitro de Vellozia sincorana Ayensu & Smith |
title_full |
Regenera??o in vitro de Vellozia sincorana Ayensu & Smith |
title_fullStr |
Regenera??o in vitro de Vellozia sincorana Ayensu & Smith |
title_full_unstemmed |
Regenera??o in vitro de Vellozia sincorana Ayensu & Smith |
title_sort |
Regenera??o in vitro de Vellozia sincorana Ayensu & Smith |
author |
Borges, B?rbara Paula dos Santos |
author_facet |
Borges, B?rbara Paula dos Santos |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Santana, Jos? Raniere Ferreira de |
dc.contributor.advisor1ID.fl_str_mv |
55155448549 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/8371650330571261 |
dc.contributor.authorID.fl_str_mv |
03268282571 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5248371924510498 |
dc.contributor.author.fl_str_mv |
Borges, B?rbara Paula dos Santos |
contributor_str_mv |
Santana, Jos? Raniere Ferreira de |
dc.subject.por.fl_str_mv |
Candomb? Micropropaga??o Reguladores vegetais Estresse h?drico Estresse t?rmico |
topic |
Candomb? Micropropaga??o Reguladores vegetais Estresse h?drico Estresse t?rmico Candomb? Micropropagation Growth regulators Water stress Heat stress BOTANICA::FISIOLOGIA VEGETAL CIENCIAS BIOLOGICAS::GENETICA |
dc.subject.eng.fl_str_mv |
Candomb? Micropropagation Growth regulators Water stress Heat stress |
dc.subject.cnpq.fl_str_mv |
BOTANICA::FISIOLOGIA VEGETAL CIENCIAS BIOLOGICAS::GENETICA |
description |
Vellozia sincorana Ayensu & SMITHis endemic of Rupestres Fields of Chapada Diamantina, popularly known as Candomb?. This species has a highly flammable resin, and the stem used to ignite wood stoves and roots to produce incense. Candomb? presents few studies in the literature, there are no studies of propagation of the species. This study aimed to develop in vitro multiplication protocol of V. sincorana, analyzing the behavior of in vitro shoots subjected to stress, and its influence on multiplication. They were used as explants in vitro shoots obtained, inoculated with concentrations of BAP (0.0; 2.22; 4.44 ?M), CIN (0.0; 2.32; 4.64 ?M) and IBA (0.0; 1.48 ?M) in MS ? medium supplemented with 87.64 mM sucrose, 1g.L-1activated carbon, and 0.7% agar (basic medium). Subsequently, it tested concentrations of cytokinins BAP or KIN (0.0; 5.0; 10.0; 15.0; 20.0 ?M). In experiments with thermal stress, flame shoots were exposed for different periods (0.0; 2.0; 4.0; 6.0; 8.0; 10 seconds), and the next tested whether the periods (0.0; 4.5; 6.0; 7.5; 9.0 min) exposure of the shoots 105 ? 1 ? C in sterilize. For drought stress basic medium was agar supplemented with different concentrations (7.0; 14.0; 21.0; 28.0 g) and the following experiment different sucrose concentrations (87.64; 175.28; 262. 92; 350.56 mM). The KIN showed greater efficacy in the multiplication in relation to BAP. The highest average for number of shoots and responsive% explants were in the periods 6, 8 or 10 seconds, being observed tolerance to water and thermal stress. |
publishDate |
2015 |
dc.date.accessioned.fl_str_mv |
2015-12-02T20:10:10Z |
dc.date.issued.fl_str_mv |
2015-08-26 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BORGES, B?rbara Paula dos Santos. Regenera??o in vitro de Vellozia sincorana Ayensu & Smith. 2015. 70 f. Disserta??o (Mestrado Acad?mico em Recursos Gen?ticos Vegetais) - Universidade Estadual de Feira de Santana, Feira de Santana, 2015. |
dc.identifier.uri.fl_str_mv |
http://localhost:8080/tede/handle/tede/277 |
identifier_str_mv |
BORGES, B?rbara Paula dos Santos. Regenera??o in vitro de Vellozia sincorana Ayensu & Smith. 2015. 70 f. Disserta??o (Mestrado Acad?mico em Recursos Gen?ticos Vegetais) - Universidade Estadual de Feira de Santana, Feira de Santana, 2015. |
url |
http://localhost:8080/tede/handle/tede/277 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
2441563959449389152 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 600 |
dc.relation.department.fl_str_mv |
-6971480722008537872 |
dc.relation.cnpq.fl_str_mv |
-289993050269505198 -5518144268585252051 |
dc.relation.sponsorship.fl_str_mv |
4767858349021390776 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Feira de Santana |
dc.publisher.program.fl_str_mv |
Mestrado Acad?mico em Recursos Gen?ticos Vegetais |
dc.publisher.initials.fl_str_mv |
UEFS |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
DEPARTAMENTO DE CI?NCIAS BIOL?GICAS |
publisher.none.fl_str_mv |
Universidade Estadual de Feira de Santana |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UEFS instname:Universidade Estadual de Feira de Santana (UEFS) instacron:UEFS |
instname_str |
Universidade Estadual de Feira de Santana (UEFS) |
instacron_str |
UEFS |
institution |
UEFS |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UEFS |
collection |
Biblioteca Digital de Teses e Dissertações da UEFS |
bitstream.url.fl_str_mv |
http://tede2.uefs.br:8080/bitstream/tede/277/2/v.final+DISSERTA%C3%87%C3%83O+B%C3%81RBARA+PAULA+DOS+SANTOS+BORGES+DOS+SANTOS+BORGES.pdf http://tede2.uefs.br:8080/bitstream/tede/277/1/license.txt |
bitstream.checksum.fl_str_mv |
b385afcd4779d54bd2a324ab4f1f5b5f bd3efa91386c1718a7f26a329fdcb468 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS) |
repository.mail.fl_str_mv |
bcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.br |
_version_ |
1796793631462391808 |