Extrato dos frutos de Spondias purpurea L. como princ?pio ativo para formula??o fitocosm?tica fotoprotetora

Detalhes bibliográficos
Ano de defesa: 2015
Autor(a) principal: Silva, Renata Vieira lattes
Orientador(a): Branco, Alexsandro
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Estadual de Feira de Santana
Programa de Pós-Graduação: Mestrado Acad?mico em Biotecnologia
Departamento: DEPARTAMENTO DE CI?NCIAS BIOL?GICAS
País: Brasil
Palavras-chave em Português:
Spondias purpurea L
Fotoprotetor
CLAE-ESI-EMn
Antioxidante
UVA/UVB
Antimicrobiano
Palavras-chave em Inglês:
Sunscreen
HPLC-ESI-EMn
Antioxidant
Antimicrobial
Spondias purpurea L.
Área do conhecimento CNPq:
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
Link de acesso: http://localhost:8080/tede/handle/tede/149
Resumo: Fruits of Spondias purpurea L. (Anacardeacea), popularly known as seriguela,are rich with phenolic compounds, which can be applied in the production of phytocosmetic. This study aimed to analyze the chemical constituents of the metanolic crude extract of S. purpurea (EBMSP) by high performance liquid chromatography coupled with mass spectrometry (HPLC-ESI-MSn) and to evaluate this extract as principle active in photoprotective formulations. In addition, the flavonoid and phenolic content, sequestration activity of free radicals by DPPH method and antimicrobial activity by the inhibition zone method, were investigated. HPLC-ESI-MSnanalyses were possible to characterize five phenolic compounds, including phenolic acids, tannins and flavonols. The extract showed phenolic content and flavonoid values at 28.68 ? 0.046 mg gallic acid equivalent/g and at 2.64 ? 0.005 mg of flavonoid quercetin equivalent/g of extract, respectively. In vitro antioxidant activity showed percentage of inhibition of 74.41 to 250 ?g/mL, a high value compared with the rutin. The EBMSP demonstrated sunscreen activity, with solar protection factor (SPF) values> 6.0 at low dilutions and UVA protection equal to the reference standards benzophenone-3 and rutin. The best results among formulations 5, 10, 20 and 30% for UVA and UVB sunscreens profile corresponded to lotion 30%, however, no formulations showed synergism with the standard benzophenone-3. To evaluate the antimicrobial activity, suggests that the EBMSP has activity against Gram-negative micro-organisms Pseudomonas aeruginosa and Salmonella dublin, without action to strains of Escherichia coli.
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spelling Branco, Alexsandro02730209514http://lattes.cnpq.br/3082409710995616Silva, Renata Vieira2015-08-03T22:07:54Z2015-03-19SILVA, Renata Vieira. Extrato dos frutos de Spondias purpurea L. como princ?pio ativo para formula??o fitocosm?tica fotoprotetora. 2015. 80 f. Disserta??o (Mestrado Acad?mico em Biotecnologia)- Universidade Estadual de Feira de Santana, Feira de Santana, 2015.http://localhost:8080/tede/handle/tede/149Fruits of Spondias purpurea L. (Anacardeacea), popularly known as seriguela,are rich with phenolic compounds, which can be applied in the production of phytocosmetic. This study aimed to analyze the chemical constituents of the metanolic crude extract of S. purpurea (EBMSP) by high performance liquid chromatography coupled with mass spectrometry (HPLC-ESI-MSn) and to evaluate this extract as principle active in photoprotective formulations. In addition, the flavonoid and phenolic content, sequestration activity of free radicals by DPPH method and antimicrobial activity by the inhibition zone method, were investigated. HPLC-ESI-MSnanalyses were possible to characterize five phenolic compounds, including phenolic acids, tannins and flavonols. The extract showed phenolic content and flavonoid values at 28.68 ? 0.046 mg gallic acid equivalent/g and at 2.64 ? 0.005 mg of flavonoid quercetin equivalent/g of extract, respectively. In vitro antioxidant activity showed percentage of inhibition of 74.41 to 250 ?g/mL, a high value compared with the rutin. The EBMSP demonstrated sunscreen activity, with solar protection factor (SPF) values> 6.0 at low dilutions and UVA protection equal to the reference standards benzophenone-3 and rutin. The best results among formulations 5, 10, 20 and 30% for UVA and UVB sunscreens profile corresponded to lotion 30%, however, no formulations showed synergism with the standard benzophenone-3. To evaluate the antimicrobial activity, suggests that the EBMSP has activity against Gram-negative micro-organisms Pseudomonas aeruginosa and Salmonella dublin, without action to strains of Escherichia coli.Os frutos de Spondias purpurea L. (Anacardeacea), conhecidos popularmente como seriguela, s?o ricos em compostos fen?licos, que podem ser aplicados na produ??o de fitocosm?ticos. Este trabalho teve como objetivo analisar os constituintes qu?micos do extrato bruto metan?lico de S. purpurea(EBMSP) por cromatografia l?quida de alta efici?ncia acoplada ? espectrometria de massas (CLAE-ESI-EMn), avaliando sua utiliza??o como princ?pio ativo em formula??es fotoprotetoras. Em adi??o, o teor de fen?licos e de flavonoides, bem como a atividade de sequestro de radicais livres pelo m?todo de DPPH e atividade antimicrobiana pelo m?todo do halo de inibi??o, tamb?m foram verificados. A an?lise por CLAE-ESI-EMn possibilitou caracterizar cinco compostos fen?licos, entre eles ?cidos fen?licos, taninos e flavon?is. O extrato apresentou valores de teor de fen?licos e flavonoides de 28,68 ? 0,046 mg de equivalente a ?cido g?lico/g e 2,64 ? 0,005 mg de flavonoide equivalente a quercetina/g de extrato, respectivamente. A atividade antioxidante in vitro demonstrou percentual de inibi??o de 74,41 a 250?g/mL, valor elevadoquando comparado com o padr?o de rutina. A avalia??o do potencial fotoprotetor in vitro do EBMSP demonstrou atividade fotoprotetora, com valores de FPS>6,0 em baixas dilui??es, e prote??o UVA igual aos padr?es de benzofenona-3 e rutina. Os melhores resultados entre as formula??es de 5, 10, 20 e 30%, para o perfil fotoprotetor UVA e UVB, corresponderam ? lo??o de 30%, entretanto, nenhuma das formula??es obteve sinergismo com o padr?o de benzofenona-3. Para avalia??o da atividade antimicrobiana, sugere-se que o EBMSP possui atividade contra os micro-organismos Gram-negativos Pseudomonas aeruginosa e Salmonella dublin, sem a??o para cepas de Escherichia coli.Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2015-08-03T22:07:54Z No. of bitstreams: 1 DISSERTA??O - RENATA VIEIRA SILVA.pdf: 1258695 bytes, checksum: 8741b07320d6f4c024d94e4aa2fd913a (MD5)Made available in DSpace on 2015-08-03T22:07:54Z (GMT). No. of bitstreams: 1 DISSERTA??O - RENATA VIEIRA SILVA.pdf: 1258695 bytes, checksum: 8741b07320d6f4c024d94e4aa2fd913a (MD5) Previous issue date: 2015-03-19application/pdfporUniversidade Estadual de Feira de SantanaMestrado Acad?mico em BiotecnologiaUEFSBrasilDEPARTAMENTO DE CI?NCIAS BIOL?GICASSpondias purpurea LFotoprotetorCLAE-ESI-EMnAntioxidanteUVA/UVBAntimicrobianoSunscreenHPLC-ESI-EMnAntioxidantAntimicrobialSpondias purpurea L.CIENCIAS BIOLOGICAS::BIOLOGIA GERALExtrato dos frutos de Spondias purpurea L. como princ?pio ativo para formula??o fitocosm?tica fotoprotetorainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis7701973706309601282600600600-6971480722008537872-1634559385931244697info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEFSinstname:Universidade Estadual de Feira de Santana (UEFS)instacron:UEFSORIGINALDISSERTA??O - RENATA VIEIRA SILVA.pdfDISSERTA??O - RENATA VIEIRA SILVA.pdfapplication/pdf763199http://tede2.uefs.br:8080/bitstream/tede/149/2/DISSERTA%C3%87%C3%83O+-+RENATA+VIEIRA+SILVA.pdf66961f744a9b2beb9c3c99fb269c3266MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://tede2.uefs.br:8080/bitstream/tede/149/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/1492015-10-09 21:44:51.654oai:tede2.uefs.br:8080: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.uefs.br:8080/PUBhttp://tede2.uefs.br:8080/oai/requestbcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.bropendoar:2015-10-10T00:44:51Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS)false
dc.title.por.fl_str_mv Extrato dos frutos de Spondias purpurea L. como princ?pio ativo para formula??o fitocosm?tica fotoprotetora
title Extrato dos frutos de Spondias purpurea L. como princ?pio ativo para formula??o fitocosm?tica fotoprotetora
spellingShingle Extrato dos frutos de Spondias purpurea L. como princ?pio ativo para formula??o fitocosm?tica fotoprotetora
Silva, Renata Vieira
Spondias purpurea L
Fotoprotetor
CLAE-ESI-EMn
Antioxidante
UVA/UVB
Antimicrobiano
Sunscreen
HPLC-ESI-EMn
Antioxidant
Antimicrobial
Spondias purpurea L.
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
title_short Extrato dos frutos de Spondias purpurea L. como princ?pio ativo para formula??o fitocosm?tica fotoprotetora
title_full Extrato dos frutos de Spondias purpurea L. como princ?pio ativo para formula??o fitocosm?tica fotoprotetora
title_fullStr Extrato dos frutos de Spondias purpurea L. como princ?pio ativo para formula??o fitocosm?tica fotoprotetora
title_full_unstemmed Extrato dos frutos de Spondias purpurea L. como princ?pio ativo para formula??o fitocosm?tica fotoprotetora
title_sort Extrato dos frutos de Spondias purpurea L. como princ?pio ativo para formula??o fitocosm?tica fotoprotetora
author Silva, Renata Vieira
author_facet Silva, Renata Vieira
author_role author
dc.contributor.advisor1.fl_str_mv Branco, Alexsandro
dc.contributor.authorID.fl_str_mv 02730209514
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/3082409710995616
dc.contributor.author.fl_str_mv Silva, Renata Vieira
contributor_str_mv Branco, Alexsandro
dc.subject.por.fl_str_mv Spondias purpurea L
Fotoprotetor
CLAE-ESI-EMn
Antioxidante
UVA/UVB
Antimicrobiano
topic Spondias purpurea L
Fotoprotetor
CLAE-ESI-EMn
Antioxidante
UVA/UVB
Antimicrobiano
Sunscreen
HPLC-ESI-EMn
Antioxidant
Antimicrobial
Spondias purpurea L.
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
dc.subject.eng.fl_str_mv Sunscreen
HPLC-ESI-EMn
Antioxidant
Antimicrobial
Spondias purpurea L.
dc.subject.cnpq.fl_str_mv CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
description Fruits of Spondias purpurea L. (Anacardeacea), popularly known as seriguela,are rich with phenolic compounds, which can be applied in the production of phytocosmetic. This study aimed to analyze the chemical constituents of the metanolic crude extract of S. purpurea (EBMSP) by high performance liquid chromatography coupled with mass spectrometry (HPLC-ESI-MSn) and to evaluate this extract as principle active in photoprotective formulations. In addition, the flavonoid and phenolic content, sequestration activity of free radicals by DPPH method and antimicrobial activity by the inhibition zone method, were investigated. HPLC-ESI-MSnanalyses were possible to characterize five phenolic compounds, including phenolic acids, tannins and flavonols. The extract showed phenolic content and flavonoid values at 28.68 ? 0.046 mg gallic acid equivalent/g and at 2.64 ? 0.005 mg of flavonoid quercetin equivalent/g of extract, respectively. In vitro antioxidant activity showed percentage of inhibition of 74.41 to 250 ?g/mL, a high value compared with the rutin. The EBMSP demonstrated sunscreen activity, with solar protection factor (SPF) values> 6.0 at low dilutions and UVA protection equal to the reference standards benzophenone-3 and rutin. The best results among formulations 5, 10, 20 and 30% for UVA and UVB sunscreens profile corresponded to lotion 30%, however, no formulations showed synergism with the standard benzophenone-3. To evaluate the antimicrobial activity, suggests that the EBMSP has activity against Gram-negative micro-organisms Pseudomonas aeruginosa and Salmonella dublin, without action to strains of Escherichia coli.
publishDate 2015
dc.date.accessioned.fl_str_mv 2015-08-03T22:07:54Z
dc.date.issued.fl_str_mv 2015-03-19
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, Renata Vieira. Extrato dos frutos de Spondias purpurea L. como princ?pio ativo para formula??o fitocosm?tica fotoprotetora. 2015. 80 f. Disserta??o (Mestrado Acad?mico em Biotecnologia)- Universidade Estadual de Feira de Santana, Feira de Santana, 2015.
dc.identifier.uri.fl_str_mv http://localhost:8080/tede/handle/tede/149
identifier_str_mv SILVA, Renata Vieira. Extrato dos frutos de Spondias purpurea L. como princ?pio ativo para formula??o fitocosm?tica fotoprotetora. 2015. 80 f. Disserta??o (Mestrado Acad?mico em Biotecnologia)- Universidade Estadual de Feira de Santana, Feira de Santana, 2015.
url http://localhost:8080/tede/handle/tede/149
dc.language.iso.fl_str_mv por
language por
dc.relation.program.fl_str_mv 7701973706309601282
dc.relation.confidence.fl_str_mv 600
600
600
dc.relation.department.fl_str_mv -6971480722008537872
dc.relation.cnpq.fl_str_mv -1634559385931244697
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual de Feira de Santana
dc.publisher.program.fl_str_mv Mestrado Acad?mico em Biotecnologia
dc.publisher.initials.fl_str_mv UEFS
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv DEPARTAMENTO DE CI?NCIAS BIOL?GICAS
publisher.none.fl_str_mv Universidade Estadual de Feira de Santana
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da UEFS
instname:Universidade Estadual de Feira de Santana (UEFS)
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collection Biblioteca Digital de Teses e Dissertações da UEFS
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