Expressão de variantes de aquaporinas em algodão submetido a estresse salino
Ano de defesa: | 2019 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Estadual da Paraíba
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Ciências Agrárias - PPGCA
|
Departamento: |
Pró-Reitoria de Pós-Graduação e Pesquisa - PRPGP
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://tede.bc.uepb.edu.br/jspui/handle/tede/3996 |
Resumo: | Salinization leads to several damages to agricultural regions at worldwide, especially in semiarid climate, reducing the crop yields. Cotton (Gossypium hirsutum L.) is a Malvaceae with wide genetic variability to salt stress. The identification of salinity tolerant genotypes in a breeding program is a dynamic target, due to selection is often based on plant phenotypes. Molecular markers are reliable tools to aid in these selection processes. It is known that aquaporins (AQPs) are channel proteins with a fundamental role in water relations and tolerance to environmental stresses. The plants have fine regulation of water transport through the activity of AQPs. In this work three AQP subtypes were adopted in order to evaluate the tolerance to saline stress, based on the expression of transcripts by qPCR. Additionally, physiological descriptors were adopted to validate the molecular findings. Seven cotton genotypes were subjected to saline stress (190 mM NaCl), initiated at V3 stage (21 days after emergence), during 72 h. At the end of stress, root tissues were collected for total RNA extraction, followed by cDNA synthesis and qPCR analyzes. At the same time, full expanded leaves were used to record the gas exchange, using IRGA, and measuring the relative water content. The dry matter of the shoots was also evaluated. Three sets of specific primers were used, drawn from NCBI sequences of GhPIP1;1, GhTIP2;1 and GhSIP1;3. The GhPP2A gene was used as a reference. From the expression pattern of the AQPs transcripts, we found that the genotypes adapted to semiarid (BRS Seridó, 7MH, CNPA MT 2009 152 and BRS 416) revealed down regulation of AQP subtypes, mainly GhPIP1;1 and GhTIP2;1 whose action are characterized as tolerance to salinity. The results of gas exchanges, relative water content and dry matter were consistent with the molecular ones, which are supported to confirm that GhPIP1;1 and GhTIP2;1 located at plasma membrane and vacuoles, respectively, could be adopted as markers for identification of cotton genotypes with tolerance to salinity. |
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Santos, Roseane Cavalcanti dos17469333487Lima, Liziane Maria de73747211453Santos, Igor Luiz Vieira de Lima0315658940309988559445Braz, Luana Camilla Cordeiro2022-01-04T13:27:22Z2019-02-28BRAZ, Luana Camilla Cordeiro. Expressão de variantes de aquaporinas em algodão submetido a estresse salino. 2019. 48 f. Dissertação (Programa de Pós-Graduação em Ciências Agrárias - PPGCA) - Universidade Estadual da Paraíba, Campina Grande, 2021.http://tede.bc.uepb.edu.br/jspui/handle/tede/3996Salinization leads to several damages to agricultural regions at worldwide, especially in semiarid climate, reducing the crop yields. Cotton (Gossypium hirsutum L.) is a Malvaceae with wide genetic variability to salt stress. The identification of salinity tolerant genotypes in a breeding program is a dynamic target, due to selection is often based on plant phenotypes. Molecular markers are reliable tools to aid in these selection processes. It is known that aquaporins (AQPs) are channel proteins with a fundamental role in water relations and tolerance to environmental stresses. The plants have fine regulation of water transport through the activity of AQPs. In this work three AQP subtypes were adopted in order to evaluate the tolerance to saline stress, based on the expression of transcripts by qPCR. Additionally, physiological descriptors were adopted to validate the molecular findings. Seven cotton genotypes were subjected to saline stress (190 mM NaCl), initiated at V3 stage (21 days after emergence), during 72 h. At the end of stress, root tissues were collected for total RNA extraction, followed by cDNA synthesis and qPCR analyzes. At the same time, full expanded leaves were used to record the gas exchange, using IRGA, and measuring the relative water content. The dry matter of the shoots was also evaluated. Three sets of specific primers were used, drawn from NCBI sequences of GhPIP1;1, GhTIP2;1 and GhSIP1;3. The GhPP2A gene was used as a reference. From the expression pattern of the AQPs transcripts, we found that the genotypes adapted to semiarid (BRS Seridó, 7MH, CNPA MT 2009 152 and BRS 416) revealed down regulation of AQP subtypes, mainly GhPIP1;1 and GhTIP2;1 whose action are characterized as tolerance to salinity. The results of gas exchanges, relative water content and dry matter were consistent with the molecular ones, which are supported to confirm that GhPIP1;1 and GhTIP2;1 located at plasma membrane and vacuoles, respectively, could be adopted as markers for identification of cotton genotypes with tolerance to salinity.A salinização provoca sérios danos a várias regiões agrícolas no mundo, principalmente as de clima semiárido, prejudicando a produtividade das culturas. O algodão (Gossypium hirsutum L.) é uma Malvaceae com grande variabilidade genética na resposta ao estresse salino. A identificação de genótipos tolerantes a salinidade é um alvo dinâmico nos trabalhos de melhoramento e a seleção é frequentemente baseada no fenótipo das plantas. Marcadores moleculares se constituem em ferramentas confiáveis para auxiliar nesses processos de seleção. Sabe-se que as aquaporinas (AQPs) são proteínas-canal com papel fundamental nas relações hídricas e na tolerância a estresses ambientais. As plantas apresentam fina regulação do transporte de água por meio da atividade das AQPs. Neste trabalho adotou-se três variantes de AQPs com objetivo de avaliar a tolerância ao estresse salino, baseando-se na expressão de transcritos por qPCR. Adicionalmente, descritores fisiológicos foram adotados para validar os achados moleculares. Sete genótipos de algodão foram submetidos a estresse salino (NaCl 190 mM), iniciado no estágio V3 (21 dias após emergência), por 72 h. Ao final do estresse, tecidos radiculares foram coletados para extração de RNA total, seguido de síntese de cDNA e análises de qPCR. Paralelamente, foram usadas folhas completamente expandidas para registro das trocas gasosas, com uso do IRGA (Analisador de gás por infravermelho) e mensuração do conteúdo relativo de água. A massa seca da parte aérea também foi avaliada. Três conjuntos de primers específicos foram usados, desenhados a partir de sequências depositadas no NCBI de GhPIP1;1, GhTIP2;1 e GhSIP1;3. O gene GhPP2A foi usado como referência. A partir do padrão de expressão dos transcritos de AQPs, foi verificado que os genótipos adaptados ao semiárido (BRS Seridó, 7MH, CNPA MT 2009 152 e BRS 416) revelaram repressão das AQPs, especialmente de GhPIP1;1 e GhTIP2;1, cuja ação é caracterizada como tolerância a salinidade. Os resultados de trocas gasosas, conteúdo relativo de água e massa seca consubstanciaram os resultados moleculares, o que fornece suporte para atestar que as variantes GhPIP1;1, GhTIP2;1, localizadas na membrana plasmática e vacúolos, respectivamente, podem ser adotadas como marcadores confiáveis para a identificação de genótipos de algodão tolerantes ao estresse salino.Submitted by Jean Medeiros (jeanletras@uepb.edu.br) on 2021-11-19T12:39:25Z No. of bitstreams: 1 PDF - Luana Camilla Cordeiro Braz.pdf: 2334977 bytes, checksum: 0dcfeab3c0a7b4db8cc83f9c22c35c95 (MD5)Approved for entry into archive by Rosalvo Andrade (rosalvo_andrade@servidor.uepb.edu.br) on 2021-12-14T19:46:19Z (GMT) No. of bitstreams: 1 PDF - Luana Camilla Cordeiro Braz.pdf: 2334977 bytes, checksum: 0dcfeab3c0a7b4db8cc83f9c22c35c95 (MD5)Made available in DSpace on 2022-01-04T13:27:22Z (GMT). No. of bitstreams: 1 PDF - Luana Camilla Cordeiro Braz.pdf: 2334977 bytes, checksum: 0dcfeab3c0a7b4db8cc83f9c22c35c95 (MD5) Previous issue date: 2019-02-28application/pdfporUniversidade Estadual da ParaíbaPrograma de Pós-Graduação em Ciências Agrárias - PPGCAUEPBBrasilPró-Reitoria de Pós-Graduação e Pesquisa - PRPGPGossypium hirsutum L.SalinidadeCotoniculturaAquaporinas vegetaisSalt stressGossypium hirsutum L.CIENCIAS AGRARIASExpressão de variantes de aquaporinas em algodão submetido a estresse salinoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-35491671506732494836006006005248714503811102787828424726906663919info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEPBinstname:Universidade Estadual da Paraíba (UEPB)instacron:UEPBORIGINALPDF - Luana Camilla Cordeiro Braz.pdfPDF - Luana Camilla Cordeiro Braz.pdfapplication/pdf2334977http://tede.bc.uepb.edu.br/jspui/bitstream/tede/3996/2/PDF+-+Luana+Camilla+Cordeiro+Braz.pdf0dcfeab3c0a7b4db8cc83f9c22c35c95MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81960http://tede.bc.uepb.edu.br/jspui/bitstream/tede/3996/1/license.txt6052ae61e77222b2086e666b7ae213ceMD51tede/39962022-01-04 10:27:22.388oai:tede.bc.uepb.edu.br:tede/3996TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkNvbSBhIGFwcmVzZW50YcOnw6NvIGRlc3RhIGxpY2Vuw6dhLCB2b2PDqiAobyBhdXRvciAoZXMpIG91IG8gdGl0dWxhciBkb3MgZGlyZWl0b3MgZGUgYXV0b3IpIGNvbmNlZGUgw6AgVW5pdmVyc2lkYWRlIApFc3RhZHVhbCBkYSBQYXJhw61iYSBvIGRpcmVpdG8gbsOjby1leGNsdXNpdm8gZGUgcmVwcm9kdXppciwgIHRyYWR1emlyIChjb25mb3JtZSBkZWZpbmlkbyBhYmFpeG8pLCBlL291IApkaXN0cmlidWlyIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyAoaW5jbHVpbmRvIG8gcmVzdW1vKSBwb3IgdG9kbyBvIG11bmRvIG5vIGZvcm1hdG8gaW1wcmVzc28gZSBlbGV0csO0bmljbyBlIAplbSBxdWFscXVlciBtZWlvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3Mgw6F1ZGlvIG91IHbDrWRlby4KClZvY8OqIGNvbmNvcmRhIHF1ZSBhIFVFUEIgcG9kZSwgc2VtIGFsdGVyYXIgbyBjb250ZcO6ZG8sIHRyYW5zcG9yIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyAKcGFyYSBxdWFscXVlciBtZWlvIG91IGZvcm1hdG8gcGFyYSBmaW5zIGRlIHByZXNlcnZhw6fDo28uCgpWb2PDqiB0YW1iw6ltIGNvbmNvcmRhIHF1ZSBhIFVFUEIgcG9kZSBtYW50ZXIgbWFpcyBkZSB1bWEgY8OzcGlhIGRhIHN1YSB0ZXNlIG91IApkaXNzZXJ0YcOnw6NvIHBhcmEgZmlucyBkZSBzZWd1cmFuw6dhLCBiYWNrdXAgZSBwcmVzZXJ2YcOnw6NvLgoKVm9jw6ogZGVjbGFyYSBxdWUgYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIMOpIG9yaWdpbmFsIGUgcXVlIHZvY8OqIHRlbSBvIHBvZGVyIGRlIGNvbmNlZGVyIG9zIGRpcmVpdG9zIGNvbnRpZG9zIApuZXN0YSBsaWNlbsOnYS4gVm9jw6ogdGFtYsOpbSBkZWNsYXJhIHF1ZSBvIGRlcMOzc2l0byBkYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIG7Do28sIHF1ZSBzZWphIGRlIHNldSAKY29uaGVjaW1lbnRvLCBpbmZyaW5nZSBkaXJlaXRvcyBhdXRvcmFpcyBkZSBuaW5ndcOpbS4KCkNhc28gYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIGNvbnRlbmhhIG1hdGVyaWFsIHF1ZSB2b2PDqiBuw6NvIHBvc3N1aSBhIHRpdHVsYXJpZGFkZSBkb3MgZGlyZWl0b3MgYXV0b3JhaXMsIHZvY8OqIApkZWNsYXJhIHF1ZSBvYnRldmUgYSBwZXJtaXNzw6NvIGlycmVzdHJpdGEgZG8gZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzIHBhcmEgY29uY2VkZXIgw6AgVUVQQiAKb3MgZGlyZWl0b3MgYXByZXNlbnRhZG9zIG5lc3RhIGxpY2Vuw6dhLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIAppZGVudGlmaWNhZG8gZSByZWNvbmhlY2lkbyBubyB0ZXh0byBvdSBubyBjb250ZcO6ZG8gZGEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIG9yYSBkZXBvc2l0YWRhLgoKQ0FTTyBBIFRFU0UgT1UgRElTU0VSVEHDh8ODTyBPUkEgREVQT1NJVEFEQSBURU5IQSBTSURPIFJFU1VMVEFETyBERSBVTSBQQVRST0PDjU5JTyBPVSAKQVBPSU8gREUgVU1BIEFHw4pOQ0lBIERFIEZPTUVOVE8gT1UgT1VUUk8gT1JHQU5JU01PIFFVRSBOw4NPIFNFSkEgQSBVRVBCLCAKVk9Dw4ogREVDTEFSQSBRVUUgUkVTUEVJVE9VIFRPRE9TIEUgUVVBSVNRVUVSIERJUkVJVE9TIERFIFJFVklTw4NPIENPTU8gVEFNQsOJTSBBUyAKREVNQUlTIE9CUklHQcOHw5VFUyBFWElHSURBUyBQT1IgQ09OVFJBVE8gT1UgQUNPUkRPLgoKQSBVRVBCIHNlIGNvbXByb21ldGUgYSBpZGVudGlmaWNhciBjbGFyYW1lbnRlIG8gc2V1IG5vbWUgKHMpIG91IG8ocykgbm9tZShzKSBkbyhzKSAKZGV0ZW50b3IoZXMpIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBkYSB0ZXNlIG91IGRpc3NlcnRhw6fDo28sIGUgbsOjbyBmYXLDoSBxdWFscXVlciBhbHRlcmHDp8OjbywgYWzDqW0gZGFxdWVsYXMgCmNvbmNlZGlkYXMgcG9yIGVzdGEgbGljZW7Dp2EuCg==Biblioteca Digital de Teses e Dissertaçõeshttp://tede.bc.uepb.edu.br/jspui/PUBhttp://tede.bc.uepb.edu.br/oai/requestbc@uepb.edu.br||opendoar:2022-01-04T13:27:22Biblioteca Digital de Teses e Dissertações da UEPB - Universidade Estadual da Paraíba (UEPB)false |
dc.title.por.fl_str_mv |
Expressão de variantes de aquaporinas em algodão submetido a estresse salino |
title |
Expressão de variantes de aquaporinas em algodão submetido a estresse salino |
spellingShingle |
Expressão de variantes de aquaporinas em algodão submetido a estresse salino Braz, Luana Camilla Cordeiro Gossypium hirsutum L. Salinidade Cotonicultura Aquaporinas vegetais Salt stress Gossypium hirsutum L. CIENCIAS AGRARIAS |
title_short |
Expressão de variantes de aquaporinas em algodão submetido a estresse salino |
title_full |
Expressão de variantes de aquaporinas em algodão submetido a estresse salino |
title_fullStr |
Expressão de variantes de aquaporinas em algodão submetido a estresse salino |
title_full_unstemmed |
Expressão de variantes de aquaporinas em algodão submetido a estresse salino |
title_sort |
Expressão de variantes de aquaporinas em algodão submetido a estresse salino |
author |
Braz, Luana Camilla Cordeiro |
author_facet |
Braz, Luana Camilla Cordeiro |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Santos, Roseane Cavalcanti dos |
dc.contributor.advisor1ID.fl_str_mv |
17469333487 |
dc.contributor.referee1.fl_str_mv |
Lima, Liziane Maria de |
dc.contributor.referee1ID.fl_str_mv |
73747211453 |
dc.contributor.referee2.fl_str_mv |
Santos, Igor Luiz Vieira de Lima |
dc.contributor.referee2ID.fl_str_mv |
03156589403 |
dc.contributor.authorID.fl_str_mv |
09988559445 |
dc.contributor.author.fl_str_mv |
Braz, Luana Camilla Cordeiro |
contributor_str_mv |
Santos, Roseane Cavalcanti dos Lima, Liziane Maria de Santos, Igor Luiz Vieira de Lima |
dc.subject.por.fl_str_mv |
Gossypium hirsutum L. Salinidade Cotonicultura Aquaporinas vegetais |
topic |
Gossypium hirsutum L. Salinidade Cotonicultura Aquaporinas vegetais Salt stress Gossypium hirsutum L. CIENCIAS AGRARIAS |
dc.subject.eng.fl_str_mv |
Salt stress Gossypium hirsutum L. |
dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS |
description |
Salinization leads to several damages to agricultural regions at worldwide, especially in semiarid climate, reducing the crop yields. Cotton (Gossypium hirsutum L.) is a Malvaceae with wide genetic variability to salt stress. The identification of salinity tolerant genotypes in a breeding program is a dynamic target, due to selection is often based on plant phenotypes. Molecular markers are reliable tools to aid in these selection processes. It is known that aquaporins (AQPs) are channel proteins with a fundamental role in water relations and tolerance to environmental stresses. The plants have fine regulation of water transport through the activity of AQPs. In this work three AQP subtypes were adopted in order to evaluate the tolerance to saline stress, based on the expression of transcripts by qPCR. Additionally, physiological descriptors were adopted to validate the molecular findings. Seven cotton genotypes were subjected to saline stress (190 mM NaCl), initiated at V3 stage (21 days after emergence), during 72 h. At the end of stress, root tissues were collected for total RNA extraction, followed by cDNA synthesis and qPCR analyzes. At the same time, full expanded leaves were used to record the gas exchange, using IRGA, and measuring the relative water content. The dry matter of the shoots was also evaluated. Three sets of specific primers were used, drawn from NCBI sequences of GhPIP1;1, GhTIP2;1 and GhSIP1;3. The GhPP2A gene was used as a reference. From the expression pattern of the AQPs transcripts, we found that the genotypes adapted to semiarid (BRS Seridó, 7MH, CNPA MT 2009 152 and BRS 416) revealed down regulation of AQP subtypes, mainly GhPIP1;1 and GhTIP2;1 whose action are characterized as tolerance to salinity. The results of gas exchanges, relative water content and dry matter were consistent with the molecular ones, which are supported to confirm that GhPIP1;1 and GhTIP2;1 located at plasma membrane and vacuoles, respectively, could be adopted as markers for identification of cotton genotypes with tolerance to salinity. |
publishDate |
2019 |
dc.date.issued.fl_str_mv |
2019-02-28 |
dc.date.accessioned.fl_str_mv |
2022-01-04T13:27:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BRAZ, Luana Camilla Cordeiro. Expressão de variantes de aquaporinas em algodão submetido a estresse salino. 2019. 48 f. Dissertação (Programa de Pós-Graduação em Ciências Agrárias - PPGCA) - Universidade Estadual da Paraíba, Campina Grande, 2021. |
dc.identifier.uri.fl_str_mv |
http://tede.bc.uepb.edu.br/jspui/handle/tede/3996 |
identifier_str_mv |
BRAZ, Luana Camilla Cordeiro. Expressão de variantes de aquaporinas em algodão submetido a estresse salino. 2019. 48 f. Dissertação (Programa de Pós-Graduação em Ciências Agrárias - PPGCA) - Universidade Estadual da Paraíba, Campina Grande, 2021. |
url |
http://tede.bc.uepb.edu.br/jspui/handle/tede/3996 |
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por |
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por |
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600 600 600 |
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524871450381110278 |
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7828424726906663919 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade Estadual da Paraíba |
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UEPB |
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Brasil |
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Pró-Reitoria de Pós-Graduação e Pesquisa - PRPGP |
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Universidade Estadual da Paraíba |
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