Efeito da fotobiomodulação simultânea dos lasers vermelho e infravermelho na cicatrização por segunda intenção de feridas cutâneas
Ano de defesa: | 2023 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , , , |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso embargado |
Idioma: | por |
Instituição de defesa: |
Universidade Estadual da Paraíba
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Odontologia - PPGO
|
Departamento: |
Pró-Reitoria de Pós-Graduação e Pesquisa - PRPGP
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://tede.bc.uepb.edu.br/jspui/handle/tede/4698 |
Resumo: | The management of skin wounds requires strategies that can accelerate the repair of injured tissue. In this regard, the use of photobiomodulation therapy (PBMT) with the use of Light Amplification by Stimulated Emission of Radiation (Laser) in the red and near infrared wavelengths stands out. Therefore, the objective of this study was to evaluate the effect of photobiomodulation with isolated and simultaneous use of red and infrared lasers in healing by secondary intention of cutaneous wounds. The sample consisted of 60 Wistar rats, aged between 70 and 90 days, weighing between 250 and 350 grams, randomly divided into 4 groups: Control (CTRL) (n=15), Red Laser (RED) (n=15) , Infrared Laser (INFRA) (n=15) and simultaneous Red and Infrared Laser (SIM) (n=15), with subgroups (n=5) for each time of euthanasia (3, 7 and 14 days). Irradiation with Red Laser ( 630±10nm, 100mW, 10.16 J/cm2 per point, for 10 seconds), Infrared Laser ( 810±10nm, 100mW, 10.16 J/cm2 per point, for 10 seconds) and simultaneous Red and Infrared Lasers ( 630±10nm and 810±10nm, 100mW for each of the lights, 10.16 J/cm2 per point, being 5.08 J/cm2 for each of the lights, for 10 seconds ), were performed immediately after wound induction, in a punctual manner, at four equidistant points around the wound and a central point, and were repeated every 48 hours, with the exception of euthanasia days. The clinical aspects of the skin wounds were evaluated and, after the euthanasia of the animals, the Wound Retraction Index (WRI) was evaluated. After removing the specimens, histological sections stained with hematoxylin and eosin were evaluated for re-epithelialization (×100) and inflammatory infiltrate (×1000) under light microscopy. The immunoexpression of TNF-α and TGF-β1 was evaluated in cells from the connective tissue and from the epithelial lining of the wounds, establishing the percentage of positive cells for each of the antibodies (×400). VERM, at 7 days, induced better re-epithelialization and a higher IRF than the other treated groups. SIM and VERM animals showed better clinical healing of the wound at 14 days. The association of lasers seems to increase the acute inflammatory response, with greater expression of TNF-α, followed by a substantial reduction in the inflammatory infiltrate. At 3 and 7 days, INFRA showed higher expression of TGF-β1 in epithelial tissue, with a statistically significant difference in relation to CTRL (p=0.01), at 3 days. The SIM and VERM groups showed a lower expression of TGF-β1, especially in the last days of the healing process. In conclusion, PBMT with the use of lasers can stimulate wound contraction, re-epithelialization and modulate inflammation, with the infrared laser inducing a greater expression of TGF-β1, in the acute phase, and the red laser and the association of two lengths waveform, presenting itself as a therapeutic possibility in the reduction of tissue fibrosis. |
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Catão, Maria Helena Chaves de Vasconceloshttps://lattes.cnpq.br/2133065990110383Pereira, Joabe dos Santoshttps://lattes.cnpq.br/6228325319211685Fernandes Neto, José de Alencarhttps://lattes.cnpq.br/4308979906059382Gomes, Daliana Queiroga de Castrohttps://lattes.cnpq.br/5687380520458665Nonaka, Cassiano Francisco Weegehttps://lattes.cnpq.br/0224522010734716Catão, Maria Helena Chaves de Vasconceloshttps://lattes.cnpq.br/2133065990110383https://lattes.cnpq.br/4339479357451457Albuquerque, Thamyres Maria Silva Simões de2023-07-28T10:54:38Z2999-12-312023-06-12ALBUQUERQUE, Thamyres Maria Silva Simões de. Efeito da fotobiomodulação simultânea dos lasers vermelho e infravermelho na cicatrização por segunda intenção de feridas cutâneas. 2023. 101 f. Tese (Programa de Pós-Graduação em Odontologia - PPGO) - Universidade Estadual da Paraíba, Campina Grande-PB, 2024.http://tede.bc.uepb.edu.br/jspui/handle/tede/4698The management of skin wounds requires strategies that can accelerate the repair of injured tissue. In this regard, the use of photobiomodulation therapy (PBMT) with the use of Light Amplification by Stimulated Emission of Radiation (Laser) in the red and near infrared wavelengths stands out. Therefore, the objective of this study was to evaluate the effect of photobiomodulation with isolated and simultaneous use of red and infrared lasers in healing by secondary intention of cutaneous wounds. The sample consisted of 60 Wistar rats, aged between 70 and 90 days, weighing between 250 and 350 grams, randomly divided into 4 groups: Control (CTRL) (n=15), Red Laser (RED) (n=15) , Infrared Laser (INFRA) (n=15) and simultaneous Red and Infrared Laser (SIM) (n=15), with subgroups (n=5) for each time of euthanasia (3, 7 and 14 days). Irradiation with Red Laser ( 630±10nm, 100mW, 10.16 J/cm2 per point, for 10 seconds), Infrared Laser ( 810±10nm, 100mW, 10.16 J/cm2 per point, for 10 seconds) and simultaneous Red and Infrared Lasers ( 630±10nm and 810±10nm, 100mW for each of the lights, 10.16 J/cm2 per point, being 5.08 J/cm2 for each of the lights, for 10 seconds ), were performed immediately after wound induction, in a punctual manner, at four equidistant points around the wound and a central point, and were repeated every 48 hours, with the exception of euthanasia days. The clinical aspects of the skin wounds were evaluated and, after the euthanasia of the animals, the Wound Retraction Index (WRI) was evaluated. After removing the specimens, histological sections stained with hematoxylin and eosin were evaluated for re-epithelialization (×100) and inflammatory infiltrate (×1000) under light microscopy. The immunoexpression of TNF-α and TGF-β1 was evaluated in cells from the connective tissue and from the epithelial lining of the wounds, establishing the percentage of positive cells for each of the antibodies (×400). VERM, at 7 days, induced better re-epithelialization and a higher IRF than the other treated groups. SIM and VERM animals showed better clinical healing of the wound at 14 days. The association of lasers seems to increase the acute inflammatory response, with greater expression of TNF-α, followed by a substantial reduction in the inflammatory infiltrate. At 3 and 7 days, INFRA showed higher expression of TGF-β1 in epithelial tissue, with a statistically significant difference in relation to CTRL (p=0.01), at 3 days. The SIM and VERM groups showed a lower expression of TGF-β1, especially in the last days of the healing process. In conclusion, PBMT with the use of lasers can stimulate wound contraction, re-epithelialization and modulate inflammation, with the infrared laser inducing a greater expression of TGF-β1, in the acute phase, and the red laser and the association of two lengths waveform, presenting itself as a therapeutic possibility in the reduction of tissue fibrosis.O manejo de feridas cutâneas requer estratégias que possam acelerar o reparo do tecido lesado. Neste aspecto, destaca-se o uso da terapia de fotobiomodulação (TFBM) com o uso do Light Amplification by Stimulated Emission of Radiation (Laser) nos comprimentos de onda vermelho e infravermelho próximo. Diante disso, o objetivo deste estudo foi avaliar o efeito da fotobiomodulação com uso isolado e simultâneo dos lasers vermelho e infravermelho na cicatrização por segunda intenção de feridas cutâneas. A amostra foi composta por 60 ratos Wistar, com idade entre 70 e 90 dias, massa entre 250 e 350 gramas, divididos aleatoriamente em 4 grupos: Controle (CTRL) (n=15), Laser Vermelho (VERM) (n=15), Laser Infravermelho (INFRA) (n=15) e Laser Vermelho e Infravermelho simultâneos (SIM) (n=15), com subgrupos (n=5) para cada tempo de eutanásia (3, 7 e 14 dias). As irradiações com o Laser Vermelho ( 630±10nm, 100mW, 10,16 J/cm2 por ponto, durante 10 segundos), Laser Infravermelho ( 810±10nm, 100mW, 10,16 J/cm2 por ponto, durante 10 segundos) e Lasers Vermelho e Infravermelho simultâneos ( 630±10nm e 810±10nm, 100mW para cada uma das luzes, 10,16 J/cm2 por ponto, sendo 5,08 J/cm2 para cada uma das luzes, durante 10 segundos), foram realizadas imediatamente após a indução das feridas, de forma pontual, em quatro pontos equidistantes ao redor da ferida e um ponto central, e se repetiram a cada 48 horas, com exceção dos dias de eutanásia. Foram avaliados os aspectos clínicos das feridas cutâneas e, após a eutanásia dos animais, foi realizada a avaliação do Índice de Retração da Ferida (IRF). Após a remoção dos espécimes, cortes histológicos corados em hematoxilina e eosina foram avaliados quanto à reepitelização (×100) e ao infiltrado inflamatório (×1000), sob microscopia de luz. A imunoexpressão de TNF-α e TGF-β1 foi avaliada em células do tecido conjuntivo e do revestimento epitelial das feridas, estabelecendo-se o percentual de células positivas para cada um dos anticorpos (×400). O VERM, aos 7 dias, induziu uma melhor reepitelização e um IRF superior aos demais grupos tratados. Os animais do SIM e VERM apresentaram uma melhor cicatrização clínica da ferida, aos 14 dias. A associação dos lasers parece aumentar a resposta inflamatória aguda, com maior expressão de TNF-α, seguida de redução substancial do infiltrado inflamatório. Aos 3 e 7 dias, o INFRA demonstrou maior expressão de TGF-β1 em tecido epitelial, com diferença estatisticamente significativa em relação ao CTRL (p=0,01), aos 3 dias. O grupo SIM e VERM apresentaram uma menor expressão de TGF-β1, principalmente nos últimos dias do processo de cicatrização. Em conclusão, a TFBM com uso dos lasers pode estimular a contração da ferida, a reepitelização e modular a inflamação, com o laser infravermelho induzindo uma maior expressão de TGF-β1, na fase aguda, e o laser vermelho e a associação de dois comprimentos de onda, apresentando-se como possibilidade terapêutica na redução da fibrose tecidual.Submitted by Thamyres Maria Silva Simões de Albuquerque (thamyres.simoes@aluno.uepb.edu.br) on 2023-07-12T13:30:00Z No. of bitstreams: 2 TS Thamyres Maria Silva Simões de Albuquerque.pdf: 2681632 bytes, checksum: a953c1bc962ba61f2075ef5f6b37464a (MD5) Termo de Depósito BDTD.pdf: 78306 bytes, checksum: bd74edf56fa0920682484f14bc021f5b (MD5)Approved for entry into archive by Jean Medeiros (jeanletras@uepb.edu.br) on 2023-07-13T14:05:33Z (GMT) No. of bitstreams: 2 TS Thamyres Maria Silva Simões de Albuquerque.pdf: 2681632 bytes, checksum: a953c1bc962ba61f2075ef5f6b37464a (MD5) Termo de Depósito BDTD.pdf: 78306 bytes, checksum: bd74edf56fa0920682484f14bc021f5b (MD5)Made available in DSpace on 2023-07-28T10:54:38Z (GMT). No. of bitstreams: 2 TS Thamyres Maria Silva Simões de Albuquerque.pdf: 2681632 bytes, checksum: a953c1bc962ba61f2075ef5f6b37464a (MD5) Termo de Depósito BDTD.pdf: 78306 bytes, checksum: bd74edf56fa0920682484f14bc021f5b (MD5) Previous issue date: 2023-06-12Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Estadual da ParaíbaPrograma de Pós-Graduação em Odontologia - PPGOUEPBBrasilPró-Reitoria de Pós-Graduação e Pesquisa - PRPGPFototerapiaCicatrizaçãoFotobiomodulaçãoWound healingPhotobiomodulationCIENCIAS DA SAUDE::ODONTOLOGIAEfeito da fotobiomodulação simultânea dos lasers vermelho e infravermelho na cicatrização por segunda intenção de feridas cutâneasinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis188746850794111162600600600524871450381110278-2070498469879244349info:eu-repo/semantics/embargoedAccessreponame:Biblioteca Digital de Teses e Dissertações da UEPBinstname:Universidade Estadual da Paraíba (UEPB)instacron:UEPBORIGINALTS - Thamyres Maria Silva Simões de Albuquerque.pdfTS - Thamyres Maria Silva Simões de Albuquerque.pdfapplication/pdf2681632http://tede.bc.uepb.edu.br/jspui/bitstream/tede/4698/2/TS+-+Thamyres+Maria+Silva+Sim%C3%B5es+de+Albuquerque.pdfa953c1bc962ba61f2075ef5f6b37464aMD52Termo de Depósito BDTD.pdfTermo de Depósito BDTD.pdfapplication/pdf78306http://tede.bc.uepb.edu.br/jspui/bitstream/tede/4698/3/Termo+de+Dep%C3%B3sito+BDTD.pdfbd74edf56fa0920682484f14bc021f5bMD53LICENSElicense.txtlicense.txttext/plain; charset=utf-81960http://tede.bc.uepb.edu.br/jspui/bitstream/tede/4698/1/license.txt6052ae61e77222b2086e666b7ae213ceMD51tede/46982023-07-28 07:55:13.69oai:tede.bc.uepb.edu.br: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede.bc.uepb.edu.br/jspui/PUBhttp://tede.bc.uepb.edu.br/oai/requestbc@uepb.edu.br||opendoar:2023-07-28T10:55:13Biblioteca Digital de Teses e Dissertações da UEPB - Universidade Estadual da Paraíba (UEPB)false |
dc.title.por.fl_str_mv |
Efeito da fotobiomodulação simultânea dos lasers vermelho e infravermelho na cicatrização por segunda intenção de feridas cutâneas |
title |
Efeito da fotobiomodulação simultânea dos lasers vermelho e infravermelho na cicatrização por segunda intenção de feridas cutâneas |
spellingShingle |
Efeito da fotobiomodulação simultânea dos lasers vermelho e infravermelho na cicatrização por segunda intenção de feridas cutâneas Albuquerque, Thamyres Maria Silva Simões de Fototerapia Cicatrização Fotobiomodulação Wound healing Photobiomodulation CIENCIAS DA SAUDE::ODONTOLOGIA |
title_short |
Efeito da fotobiomodulação simultânea dos lasers vermelho e infravermelho na cicatrização por segunda intenção de feridas cutâneas |
title_full |
Efeito da fotobiomodulação simultânea dos lasers vermelho e infravermelho na cicatrização por segunda intenção de feridas cutâneas |
title_fullStr |
Efeito da fotobiomodulação simultânea dos lasers vermelho e infravermelho na cicatrização por segunda intenção de feridas cutâneas |
title_full_unstemmed |
Efeito da fotobiomodulação simultânea dos lasers vermelho e infravermelho na cicatrização por segunda intenção de feridas cutâneas |
title_sort |
Efeito da fotobiomodulação simultânea dos lasers vermelho e infravermelho na cicatrização por segunda intenção de feridas cutâneas |
author |
Albuquerque, Thamyres Maria Silva Simões de |
author_facet |
Albuquerque, Thamyres Maria Silva Simões de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Catão, Maria Helena Chaves de Vasconcelos |
dc.contributor.advisor1Lattes.fl_str_mv |
https://lattes.cnpq.br/2133065990110383 |
dc.contributor.referee1.fl_str_mv |
Pereira, Joabe dos Santos |
dc.contributor.referee1Lattes.fl_str_mv |
https://lattes.cnpq.br/6228325319211685 |
dc.contributor.referee2.fl_str_mv |
Fernandes Neto, José de Alencar |
dc.contributor.referee2Lattes.fl_str_mv |
https://lattes.cnpq.br/4308979906059382 |
dc.contributor.referee3.fl_str_mv |
Gomes, Daliana Queiroga de Castro |
dc.contributor.referee3Lattes.fl_str_mv |
https://lattes.cnpq.br/5687380520458665 |
dc.contributor.referee4.fl_str_mv |
Nonaka, Cassiano Francisco Weege |
dc.contributor.referee4Lattes.fl_str_mv |
https://lattes.cnpq.br/0224522010734716 |
dc.contributor.referee5.fl_str_mv |
Catão, Maria Helena Chaves de Vasconcelos |
dc.contributor.referee5Lattes.fl_str_mv |
https://lattes.cnpq.br/2133065990110383 |
dc.contributor.authorLattes.fl_str_mv |
https://lattes.cnpq.br/4339479357451457 |
dc.contributor.author.fl_str_mv |
Albuquerque, Thamyres Maria Silva Simões de |
contributor_str_mv |
Catão, Maria Helena Chaves de Vasconcelos Pereira, Joabe dos Santos Fernandes Neto, José de Alencar Gomes, Daliana Queiroga de Castro Nonaka, Cassiano Francisco Weege Catão, Maria Helena Chaves de Vasconcelos |
dc.subject.por.fl_str_mv |
Fototerapia Cicatrização Fotobiomodulação |
topic |
Fototerapia Cicatrização Fotobiomodulação Wound healing Photobiomodulation CIENCIAS DA SAUDE::ODONTOLOGIA |
dc.subject.eng.fl_str_mv |
Wound healing Photobiomodulation |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE::ODONTOLOGIA |
description |
The management of skin wounds requires strategies that can accelerate the repair of injured tissue. In this regard, the use of photobiomodulation therapy (PBMT) with the use of Light Amplification by Stimulated Emission of Radiation (Laser) in the red and near infrared wavelengths stands out. Therefore, the objective of this study was to evaluate the effect of photobiomodulation with isolated and simultaneous use of red and infrared lasers in healing by secondary intention of cutaneous wounds. The sample consisted of 60 Wistar rats, aged between 70 and 90 days, weighing between 250 and 350 grams, randomly divided into 4 groups: Control (CTRL) (n=15), Red Laser (RED) (n=15) , Infrared Laser (INFRA) (n=15) and simultaneous Red and Infrared Laser (SIM) (n=15), with subgroups (n=5) for each time of euthanasia (3, 7 and 14 days). Irradiation with Red Laser ( 630±10nm, 100mW, 10.16 J/cm2 per point, for 10 seconds), Infrared Laser ( 810±10nm, 100mW, 10.16 J/cm2 per point, for 10 seconds) and simultaneous Red and Infrared Lasers ( 630±10nm and 810±10nm, 100mW for each of the lights, 10.16 J/cm2 per point, being 5.08 J/cm2 for each of the lights, for 10 seconds ), were performed immediately after wound induction, in a punctual manner, at four equidistant points around the wound and a central point, and were repeated every 48 hours, with the exception of euthanasia days. The clinical aspects of the skin wounds were evaluated and, after the euthanasia of the animals, the Wound Retraction Index (WRI) was evaluated. After removing the specimens, histological sections stained with hematoxylin and eosin were evaluated for re-epithelialization (×100) and inflammatory infiltrate (×1000) under light microscopy. The immunoexpression of TNF-α and TGF-β1 was evaluated in cells from the connective tissue and from the epithelial lining of the wounds, establishing the percentage of positive cells for each of the antibodies (×400). VERM, at 7 days, induced better re-epithelialization and a higher IRF than the other treated groups. SIM and VERM animals showed better clinical healing of the wound at 14 days. The association of lasers seems to increase the acute inflammatory response, with greater expression of TNF-α, followed by a substantial reduction in the inflammatory infiltrate. At 3 and 7 days, INFRA showed higher expression of TGF-β1 in epithelial tissue, with a statistically significant difference in relation to CTRL (p=0.01), at 3 days. The SIM and VERM groups showed a lower expression of TGF-β1, especially in the last days of the healing process. In conclusion, PBMT with the use of lasers can stimulate wound contraction, re-epithelialization and modulate inflammation, with the infrared laser inducing a greater expression of TGF-β1, in the acute phase, and the red laser and the association of two lengths waveform, presenting itself as a therapeutic possibility in the reduction of tissue fibrosis. |
publishDate |
2023 |
dc.date.accessioned.fl_str_mv |
2023-07-28T10:54:38Z |
dc.date.issued.fl_str_mv |
2023-06-12 |
dc.date.available.fl_str_mv |
2999-12-31 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
ALBUQUERQUE, Thamyres Maria Silva Simões de. Efeito da fotobiomodulação simultânea dos lasers vermelho e infravermelho na cicatrização por segunda intenção de feridas cutâneas. 2023. 101 f. Tese (Programa de Pós-Graduação em Odontologia - PPGO) - Universidade Estadual da Paraíba, Campina Grande-PB, 2024. |
dc.identifier.uri.fl_str_mv |
http://tede.bc.uepb.edu.br/jspui/handle/tede/4698 |
identifier_str_mv |
ALBUQUERQUE, Thamyres Maria Silva Simões de. Efeito da fotobiomodulação simultânea dos lasers vermelho e infravermelho na cicatrização por segunda intenção de feridas cutâneas. 2023. 101 f. Tese (Programa de Pós-Graduação em Odontologia - PPGO) - Universidade Estadual da Paraíba, Campina Grande-PB, 2024. |
url |
http://tede.bc.uepb.edu.br/jspui/handle/tede/4698 |
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Universidade Estadual da Paraíba |
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UEPB |
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Pró-Reitoria de Pós-Graduação e Pesquisa - PRPGP |
publisher.none.fl_str_mv |
Universidade Estadual da Paraíba |
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