Efeito crônico da administração de esteróide anabólico androgênico na próstata de ratos

• Ano de defesa: 2013
• Autor(a) principal: Vargas, Rafael Arêas
• Orientador(a): Favorito, Luciano Alves
• Banca de defesa: Costa, Waldemar Silva , Gregório, Bianca Martins , Javaroni, Valter
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade do Estado do Rio de Janeiro
• Programa de Pós-Graduação: Programa de Pós-Graduação em Fisiopatologia e Ciências Cirúrgicas
• Departamento: Centro Biomédico::Faculdade de Ciências Médicas
• País: BR
• Palavras-chave em Português: Próstata; Anabolizantes esteróides; Nandrolona; Morfometria; Próstata - Efeito de drogas; Esteróides anabólicos; Nandrolona - Efeitos adversos; Exercícios - Aspectos fisiológicos; Anabolizantes - Efeitos adversos
• Palavras-chave em Inglês: Prostate; Anabolic-steroid; Nandrolone; Morphometry; Prostate - Effect of drugs; Anabolic steroids; Nandrolone - Adverse effects; Exercise - Physiological aspects; Steroids - Adverse effects
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::MORFOLOGIA
• Link de acesso: http://www.bdtd.uerj.br/handle/1/12349

Anabolic-androgenic steroids (AAS) are used by professional and amateur athletes with the goal of improving athletic ability, appearance and muscle mass. Many adverse effects have been associated with AAS abuse, including disorders of the urogenital tract. This study aimed to assess the morphological modifications in the prostate ventral lobe of pubertal and adult rats chronically treated with supraphysiological doses of AAS using morphometric methods. We studied 39 male Wistar rats weighing between 400 and 550 g. The rats was assigned into four groups: (a) control rats, with 105 days of age (C105) (n = 7); (b) control rats with 65 days of age (C65) (n = 9), injected only with the vehicle (peanut oil); (c) treated rats, with 105 days of age (T105) (n = 10) and (d) treated rats with 65 days of age (T65) (n = 13). The treated rats were injected with nandrolone decanoate at a dose of 10 mg.Kg-1 body weight. The steroid hormone and the vehicle were administered by intramuscular injection once a week for eight weeks. The rats were killed at 161 days of age (C105 and T105) and 121 days of age (C65 and T65) and ventral lobe of the prostate was dissected and processed for histology. The height of the acinar epithelium was measured in micrometers in photomicrographs of 400x magnification. The surface densities of the lumen, epithelium and stroma were calculated by the point intercepts method, with a grid of 100 points superimposed on 200x magnified images. All analyses were performed with the ImageJ software. Student's-t-test and One-way ANOVA was used for mean comparisons. For all cases, significance was set at 0.05. The weight and volume of the prostate ventral lobe showed differences between groups C65, T65, C105 and T105. The epithelium height showed no difference between groups C65 and T65 (p=0.8509), but the T105 group showed an increase of 32% compared to C105 (p=0.0089). The lumen surface density presented no difference between C65 and T65 (p=0.9031) but a decrease of 19% in T105 animals compared to C105 (p=0.0061). There was no difference in epithelium surface density between C65 and T65 (p=0.7375), but was group T105 had a 51% increase (p=0.0065) in comparison to C105. Regarding stroma surface density, there were no differences between C65 and T65 or between C105 and T105. Finally, there was no difference in collagen pattern between C105 and T105, but T65 showed a predominance of collagen fibers type I compared to C65. The use of anabolic androgenic steroids in rats promotes structural changes in the prostate. We observed structural changes in the weight, volume and epithelium height of the prostate ventral lobe and a predominance of collagen fibers.