Efeitos benéficos do tratamento com óleo de peixe em camundongos c57bl/6 alimentados com dieta hiperlipídica e rica em sacarose

• Ano de defesa: 2008
• Autor(a) principal: Silva, Sandra Barbosa da
• Orientador(a): Lacerda, Márcia Barbosa Águila Mandarim de
• Banca de defesa: Mulder, Alessandra da Rocha Pinheiro , Dôres, Sílvia Maria Custódio das
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade do Estado do Rio de Janeiro
• Programa de Pós-Graduação: Programa de Pós-Graduação em Biologia Humana e Experimental
• Departamento: Centro Biomédico::Instituto de Biologia Roberto Alcantara Gomes
• País: BR
• Palavras-chave em Português: Óleo de peixe; Obesidade; Dieta hiperlipídica; Dieta rica em sacarose; Camundongos C57BL/6
• Palavras-chave em Inglês: Fish oil; Obesity; High fat diet; High sucrose diet; Mice C57BL/6
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::MORFOLOGIA
• Link de acesso: http://www.bdtd.uerj.br/handle/1/7820

To evaluate the fish oil treatment upon morphological and metabolic changes in the pancreas and adipose tissue of C57BL/6 mice fed high-fat-high-sucrose (HFHS) diet. Male C57BL/6 mice were fed HFHS chow or standard chow (SC). At 3 mo-old, HFHS mice were separated into untreated group (HFHS) or treated with fish oil (HFHS-Fo, 1.5 g/kg/day). At 4-mo-old animals were sacrificed. HFHS had increase in body mass (BM) and in total body fat, but HFHS-Fo had smaller BM and total body fat in relation to HFHS. Plasma glucose and insulin levels were not affected among the groups, but HFHS and HFHS-Fo had higher HOMA-IR ratio. HFHS and HFHS-FO had increased plasma total cholesterol and LDL-C, but HFHS-Fo increased plasma HDL-C and decreased triglycerides levels. The adipocytes size were greater in HFHS, when compared to SC and HFHS-Fo groups. HFHS-Fo had smaller pancreas mass and HFHS presented higher islet pancreatic diameter, when compared to SC group. SC group showed strong expression for insulin, glucagon and GLUT-2 in all pancreatic islets, and HFHS presented lesser expression for GLUT-2. However, HFHS-Fo presented increase of GLUT-2 expression. Fish oil treatment was able to reduce body mass gain and plasma TG, reduce fat pad adiposity as well as adipocyte and pancreatic islet hypertrophy, prevent decrease GLUT-2 in C57BL/6.