Dietas hiperlipídicas alteram citocinas inflamatórias, adipocinas, tecido adiposo e fígado em camundongos

• Ano de defesa: 2011
• Autor(a) principal: Pereira, Mariana Catta-preta Caiado
• Orientador(a): Lacerda, Márcia Barbosa Águila Mandarim de
• Banca de defesa: Mandarim-de-Lacerda, Carlos Alberto , Brunini, Tatiana Marlowe Cunha , Vianna, Lúcia Marques Alves , Dôres, Sílvia Maria Custódio das
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade do Estado do Rio de Janeiro
• Programa de Pós-Graduação: Programa de Pós-Graduação em Biologia Humana e Experimental
• Departamento: Centro Biomédico::Instituto de Biologia Roberto Alcantara Gomes
• País: BR
• Palavras-chave em Português: Dieta hiperlipídica; Óleo de canola; Óleo de oliva; Óleo de girassol; Banha de porco; Lipídios Metabolismo; Gorduras na dieta Metabolismo; Gorduras na dieta Administração e dosagem; Óleos vegetais; Fígado Metabolismo
• Palavras-chave em Inglês: High-fat diet; Canola oil; Olive oil; Sunflower oil; Lard
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::MORFOLOGIA
• Link de acesso: http://www.bdtd.uerj.br/handle/1/7773

Diets rich in saturated fat have a deleterious effect on glucose metabolism, adipokine release and inflammation. However, other lipid sources may modulate these parameters differently. Thus, the aim of the present work was to investigate the effect of high-fat diets from different sources on carbohydrate and lipid metabolism, adipose tissue and liver. Male C57BL/6 mice were allocated into 5 groups (n=10/group): standard chow fed mice (SC, 10% lipid, control group), and mice fed with high-fat diets (HF, 60% lipids) as follow: lard (HF-L), olive oil (HF-O), sunflower oil (HF-S), and canola oil (HF-C). Diets were offered for 10 weeks. Food intake was recorded daily and body mass weekly. One week before euthanasia, fasting glucose and intraperitoneal insulin tolerance test (IPITT) were assessed. At euthanasia, blood was collected, and the liver and adipose tissue were weighed. Insulin, leptin, adiponectin, resistin, tumoral necrosis factor alpha (TNFα), interlekin-6 (IL-6), monocyte chemoattractant protein-1 (MCP-1), and plasminogen activator inhibitor-1 (PAI-1) were measured by an Elisa assay. The HOMA-IR index was calculated based on fasting glucose and insulin. Insulin, resistin, leptin and HOMA-IR were higher in HF-L and HF-O groups compared with the remainder groups (P < 0.0001). IL-6 was higher in the HF-L group compared with the other groups (P < 0.0005), whereas adiponectin levels were the lowest (P < 0.0001). The amount of both subcutaneous and visceral fat depots was the highest in the HF-L group, and they also had increased adipocyte diameter. However, visceral to subcutaneous fat ratio was higher in HF-L and HF-O groups compared with the remainder groups. Additionally, HF-L and HF-O groups showed increased hepatic triglyceride content and steatosis. Our data allow us to conclude that the type of lipid instead of its amount is important for determining adipose tissue distribution, carbohydrate metabolism, hepatic triglyceride accumulation and steatosis in mice fed a high-fat diet.