Efeito da deficiência de tiamina sobre a inflamação, estresse oxidante e migração celular em modelo experimental de sepse

• Ano de defesa: 2012
• Autor(a) principal: Andrade, José Antenor Araújo de
• Orientador(a): Cunha, Sérgio da
• Banca de defesa: Lacerda, Márcia Barbosa águila Mandarim de , Japiassu, Andre Miguel
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade do Estado do Rio de Janeiro
• Programa de Pós-Graduação: Programa de Pós-Graduação em Biologia Humana e Experimental
• Departamento: Centro Biomédico::Instituto de Biologia Roberto Alcantara Gomes
• País: BR
• Palavras-chave em Português: Tiamina; Sepse; Estresse oxidante; Inflamação; Modelo de sepse
• Palavras-chave em Inglês: Thiamine; Sepsis; Oxidative stress; Inflammation; Sepsis model
• Área do conhecimento CNPq: CNPQ::CIENCIAS DA SAUDE::MEDICINA
• Link de acesso: http://www.bdtd.uerj.br/handle/1/7836

Sepsis is a prevalent condition in critically ill patients and may be associated with thiamine deficiency (TD). Since thiamine is important for energy production, redactor power recovering and ribosis and desoxirribosis synthesis, the aim of this study was to evaluate TD effect in inflammation, oxidative stress and cell recruitment in a sepsis model. Thiamine pyrophosphate (TPP) normal concentration was determined in blood of c57bl6 mice fed with AIN93G chow and also in animals fed with similar AIN93G chow without thiamine in order to determine the time required to produce TD. Another experiment was carried out with cecal ligation and puncture (CLP) as the sepsis model with four groups: SHAM with AIN93G complete chow, SHAM with TD chow, CLP with AIN93G complete chow and CLP with TD chow. TPP blood concentrations were determined by HPLC/Fluorescence. Blood and peritoneal liquid TNF-alpha, IL-1, IL-6, KC and MCP-1/CCL2 determinations were performed by ELISA. The expression of 4-HNE was performed by Western Blot. Blood and peritoneal liquid leukocytes counting were performed by optical microscopy. Peritoneal liquid bacterial concentration was determined. Blood TPP mean concentration in AIN93G complete chow was 287.9 ± 27.8 nM and TD occurred in 10 days in TD chow group and blood concentration in this group was 12.5 ± 2.4 nM. Peritoneal liquid TNF-alpha and MCP-1 concentrations were significantly greater in CLP with TD chow group than the other groups. Blood IL1-β was lower in the same group. Liver 4-HNE expression was highest in TD groups. Blood mononuclear number was greater in CLP TD chow group the others. Peritoneal liquid leukocites, mononuclears and neutrophils numbers were detected in greater number in the same group. However, peritoneal liquid bacterial concentration was significantly lower in the same group. In coclusion TD was associated with greater bacterial killing in peritoneal liquid, greater oxidative stress and change in inflammatory response.