Influência do laser vermelho de baixa potência no processo de reparo de queimaduras de segundo grau

• Ano de defesa: 2015
• Autor(a) principal: Trajano, Eduardo Tavares Lima
• Orientador(a): Costa, Andréa Monte Alto
• Banca de defesa: Parizotto, Nivaldo Antônio , Paoli, Flávia de , Pôrto, Luís Cristóvão de Moraes Sobrino , Souza, Bruna Romana de
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade do Estado do Rio de Janeiro
• Programa de Pós-Graduação: Programa de Pós-Graduação em Biologia Humana e Experimental
• Departamento: Centro Biomédico::Instituto de Biologia Roberto Alcantara Gomes
• País: BR
• Palavras-chave em Português: Laser terapêutico de baixa potência; Queimadura; Cicatrização de ferida; Reparo de DNA; Cicatrização de feridas; Terapia a Laser de Baixa Potência; QueimaduraS
• Palavras-chave em Inglês: Low-level red laser; Burn; Wound healing; DNA repair
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR
• Link de acesso: http://www.bdtd.uerj.br/handle/1/7794

The aim of this study was to investigate the effects of low-level red laser on tissue repair in rats submitted to second degree burn, evaluating if the timing of laser treatment influence on the healing process and investigate the expression of the genes involved with DNA repair by base excision and nucleotide excision pathways. The animals had their backs shaved and divided as follows: control group (n = 12) - rats burned and not irradiated, early laser group (n = 12) - rats burned and irradiated from day 1 after injury for 5 consecutive days, and late laser group (n = 14) - rats burned and irradiated from day 4 after injury for 5 consecutive days. Laser irradiation was according to a clinical protocol (20 J/cm2, 100 mW, continuous wave emission mode, 660 nm) as recommended by the laser device manufacturer. Half of the animals were sacrificed 10 days after burn and the other animals were sacrificed 21 days after burn. Late laser group accelerated wound contraction 10 and 21 days after burn. Late laser group accelerated reepithelialization 18 days after burn. Late laser group increased the granulation tissue 10 and 21 days after burn. Both irradiated groups increased type III collagen expression and TGF-β 21 days after burn. Both irradiated groups increased macrophages and myofibroblasts number 10 days after burn, and decreased 21 after days. Low-level red laser exposure reduced the expression of APE1 messenger (mRNA), increased the expression of OGG1, reduced the expression of XPC and increased the expression of XPA in both irradiated groups laser and later laser groups. Low-level red laser exposure contributes to the process of tissue repair of second degree burns, suggesting that the intervention during proliferative phase is crucial in the final outcome of the repair process. Moreover, the therapeutic laser was capable of altering the expression of genes involved in DNA repair.