Desenvolvimento de metodologias para a determinação da atividade biogênica de bactérias redutoras de sulfato

• Ano de defesa: 2015
• Autor(a) principal: Queiroz, Juliana Cristina de
• Orientador(a): Costa, Antonio Carlos Augusto da
• Banca de defesa: Rio, Mônica Aquino Pires do , Oliveira, Estela Maria de , Marques, Mônica Regina da Costa , Merçon, Fábio
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade do Estado do Rio de Janeiro
• Programa de Pós-Graduação: Programa de Pós-Graduação em Química
• Departamento: Centro de Tecnologia e Ciências::Instituto de Química
• País: BR
• Palavras-chave em Português: Água de formação; bactérias redutoras de sulfato; sulfeto; cinética de crescimento; biocorrosão; radiorespirometria
• Palavras-chave em Inglês: Formation water; sulfate-reducing bacteria; sulfide; growth kinetics; biocorrosion; radiorespirometry
• Área do conhecimento CNPq: CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA
• Link de acesso: http://www.bdtd.uerj.br/handle/1/15638

Corrosion caused by biogenic H2S often results in extensive damage, being one of the main problems of petroleum industry. The objective of the present work was to evaluate microbial growth parameters and apply methodologies for sulfide detection by spectrometric at visible light and radiorespirometric techniques for estimate the metabolic activity, correlating with population of Sulfate Reducing Bacteria, through the More Probable Number (MPN) technique. Samples of formation water and SBR consortium were evaluated through drag of stable sulfides biogenically produced and quantified by spectrometry. The calculations of instant and specific rates of sulfide production allow evaluating how some microbial growth parameters may affect the SRB metabolism. The detection of trace concentrations of biogenic sulfides, undetectable by spectrometry technique, may be realized by radiorespirometric assays. For this step, serial dilutions of synthetic seawater with three distinct samples were evaluated. The realized test indicates that increasing the time of incubation of a mixed anaerobic microbial culture contributed to an increase in the capabilities of sulfate reduction, as well as the amount of carbon source. Both techniques proved to be a rapid test for the detection of biogenic sulfides, particularly those associated to corrosion products, being an useful tool for monitoring and controlling oil/water storage tanks, petroleum continental platforms and several types of reservoirs. The present work provides the continuous of the experiments, using a bigger universe of samples of petroleum industry and less spaced measuring of spectrometric technique, further the radiorespirometric evaluation in continuous mode, avoiding the H2S inhibitory effects