Avaliação dos marcadores de estresse oxidativo no modelo de programação pelo óleo de linhaça durante a lactação

• Ano de defesa: 2016
• Autor(a) principal: Soares, Patrícia Novaes
• Orientador(a): Figueiredo, Mariana Sarto
• Banca de defesa: Lopes, Gustavo Casimiro , Torsoni, Adriana Souza , Torsoni, Marcio Alberto
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade do Estado do Rio de Janeiro
• Programa de Pós-Graduação: Programa de Pós-Graduação em Fisiopatologia Clínica e Experimental
• Departamento: Centro Biomédico::Faculdade de Ciências Médicas
• País: BR
• Palavras-chave em Português: Óleo de semente do linho; Ácidos Graxos Ômega-3; Lactação; Estresse oxidativo
• Palavras-chave em Inglês: Linseed Oil; Fatty acids; Omega-3; Lactation; Oxidative stress
• Área do conhecimento CNPq: CNPQ::CIENCIAS DA SAUDE::MEDICINA::CLINICA MEDICA::ENDOCRINOLOGIA
• Link de acesso: http://www.bdtd.uerj.br/handle/1/12704

The continuous search for anti-obesity therapies shows that rich sources of ω-3 have relevant impact on weight loss. Due to the health beneficial effect, lactating women may increase the ingestion of ω-3, without scientific evidence of its safety during this critical period. Thus, we aimed to evaluate the long-term effects of maternal intake of two hyperlipidic diets during lactation on redox balance in the offspring at 21 (PN21) and 180 (PN180) days old. Thirty female Wistar rats (3 months old), were mated and after birth each litter was adjusted to 8 pups (four male and four female) and randomly assigned to one of the following groups (n=10): control (C), receiving a diet with 7% of soybean oil; hyperlipidic soybean oil (HS), receiving a diet with 19% of soybean oil; and hyperlipidic flaxseed oil (HF), receiving a diet with 17% of flaxseed oil + 2% soybean oil. The redox balance was analyzed in plasma, liver and adipose tissue from dams and offspring at PN21 and PN180. The HS dams had higher malondialdehyde (MDA) in plasma and HF dams had higher plasma MDA and lower plasma superoxide dismutase (SOD), with higher SOD and protein carbonylation in visceral adipose tissue (VAT). At PN21, male HS offspring showed higher protein carbonylation in plasma and liver and male HF had higher plasma glutathione peroxidase (GPx). At PN180, male HS offspring had higher liver protein carbonylation and MDA and male HF showed higher catalase (CAT) in plasma and higher protein carbonylation, MDA and CAT in VAT. At PN21 females showed lower plasma CAT with higher liver protein carbonylation in HS group, while plasma CAT was higher in HF group. At PN180, only plasma CAT was higher in HS group. Thus, both hyperlipidic diets can program for different deleterious effects, when used during lactation. HS males and females offspring had higher deleterious effects in liver, while HF male had higher oxidative damage in VAT.