Farinha do caroço de açaí (Euterpe oleracea Mart.): caracterização e efeitos da adição desta farinha em modelo murino de obesidade induzida por dieta

• Ano de defesa: 2016
• Autor(a) principal: Silva, Renata Cristina da
• Orientador(a): Resende, Angela de Castro
• Banca de defesa: Boaventura, Gilson Teles , Souza-mello, Vanessa de
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade do Estado do Rio de Janeiro
• Programa de Pós-Graduação: Programa de Pós-Graduação em Biociências
• Departamento: Centro Biomédico::Instituto de Biologia Roberto Alcantara Gomes
• País: BR
• Palavras-chave em Português: Açaí; Compostos bioativos; Obesidade; Açaí Uso terapêutico; Nutrição Metabolismo
• Palavras-chave em Inglês: Açaí; Bioactive compounds; Obesity
• Área do conhecimento CNPq: CNPQ::CIENCIAS DA SAUDE::NUTRICAO
• Link de acesso: http://www.bdtd.uerj.br/handle/1/16295

Obesity is a major public health problem, mainly by being associated with the appearance of different comorbidities. Several foods have been used as a strategy to minimize the deleterious effects of obesity. Açai, a typical Brazilian fruit, has shown great potential with beneficial health effects. Part of the fruit, about 85%, is composed of the stone that is discarded causing substantial environmental impact. The aim of this study was to characterize the açai seed flour (ASF) and investigate the effects of dietary addition of this flour in the diet-induced obesity in mice. Male mice C57BL/6 of 21 days-old were divided into five groups: standard diet (CG); standard diet with ASF (CA30, standard diet containing 30% ASF); high fat diet (HFD); high fat diets with ASF (HFA15 and HFA30, fat diet containing 15% or 30% ASF, respectively) for 12 weeks. Characterization of ASF was performed and were assessed food intake, weight gain, biochemical effects of nutritional markers status and toxicity, effect on lipid metabolism, proteins expression of lipogenesis, cholesterol metabolism and morphology of fat and liver tissues. For analysis of variance was used one way ANOVA or Kruskall-Wallis with statistical significance level of p < 0.05. ASF contains 91.1% carbohydrate, 6.7% protein, 2.2% lipids, and 9.6 % fiber (9.3% insoluble fibers and soluble fibers 0.3%). Total polyphenols of ASF was 19.95 ± 2.7 mg GAE.g-1. Animals receiving the high fat diet were overweight, with larger amount of fat mass, negative effects on biochemical parameters of nutritional status and toxic, higher concentrations of glucose, insulin, leptin and increased insulin resistance index, altered lipid profile, presence of hepatic steatosis and increased hypertrophic obesity. ASF showed protective effect upon weight gain and acted preventing insulin resistance and improves the lipid profile of animals, which had lower concentrations of glucose, insulin and leptin. Thus, ASF reduced lipogenesis preventing the development of fatty liver and hypertrophic obesity. It was also observed influence of consumption of ASF in the modulation of proteins involved in synthesis and excretion of cholesterol. The high fecal weight in the ASF group demonstrates a significant action of fibers in the excretion of bile acids and cholesterol. Consumption of ASF also showed important results in CA30 group, indicating that its use brings benefits in the low lipids diet. Biochemical parameters of nutritional status and toxicity were not altered with the ASF use, suggesting no toxic or harmful effects to the nutritional status in the animals and that the preventive use of FCA in development of obesity and its comorbidities include action in both groups.