Estudo do potencial de remoção de substâncias húmicas na água com aplicação de enzimas fúngicas

• Ano de defesa: 2011
• Autor(a) principal: CAMPOS, Rykelly Faria
• Orientador(a): SANTIAGO, Mariângela Fontes
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Goiás
• Programa de Pós-Graduação: Mestrado em Engenharia do Meio Ambiente
• Departamento: Engenharias
• País: BR
• Palavras-chave em Português: ácidos húmico; ácidos fúlvico; fungos de decomposição branca
• Palavras-chave em Inglês: humic acid; fulvic acid; white decomposition by fungi
• Área do conhecimento CNPq: CNPQ::ENGENHARIAS::ENGENHARIA SANITARIA::SANEAMENTO BASICO
• Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tde/623

The humic substance (SHs), organic matter in different stages of degradation are formed during the decomposition of plant and animal residues in the environment, it is important to remove them, as they can give undesirable characteristics to the water distributed. The use of microorganisms such as fungi, for this removal are extremely important, since their role in sanitation is to act on processing organic waste, where they function as recyclers of matter in different ecosystems. This work aimed to study the production of phenol oxidase enzymes, peroxidases white decomposition by fungi (Trametes villosa and Pycnoporus sanguineus) for the removal of HSs in the water. The work was divided into parts: extracted, purified and characterized the humic and fulvic acids, was placed just after the HSs in water and we used the fungal enzymes for their removal, tests were also performed in the Jar Test. Analysis of the composition of HSs (characterization) and verification of degradation was performed by spectrophotometry in the ultraviolet and visible region, and infrared spectroscopy, by determining the enzyme production. The extraction method used was effective in the separation of humic and fulvic acids, the spectra of extracted humic acids showed carboxylic groups, alkyl, aromatic, alcohol, phenol and carbohydrates. The predominant enzyme in all fungal species used was laccase (Lcc); analyzed samples of Trametes villosa showed the largest decrease in absorbance at several wavelengths in the ultraviolet / visible (UVVis) spectra, an indirect measure of degradation. The samples containing the fungus Trametes villosa were also those that had a higher laccase enzyme production in most trials, reaching a production of 3.48 mL-1 with humic acid from Sigma-Aldrich® brand and 2.61 U.mL-1 for humic acid extracted and getting a percentage rate of removal of 56% and 42% respectively of these acids. In tests with fulvic acid and fungi at different pHs (3.0, 5.0 and 7.0), all has decreased range of UV/Vis with percentage removal rates of 92%. T villosa showed the higher rates of adsorption evidenced byUV-Vis spectrum, albeit to a lesser extent than those treated withenzymes, their highest percentage of adsorption was 86%. Fungi in this study have great potential for degradation and adsorption of humic substances present in water.