Relação entre a presença de macrófagos no microambiente tumoral e fatores prognósticos em câncer de mama

• Ano de defesa: 2018
• Autor(a) principal: Labre, Luciana Vieira Queiroz
• Orientador(a): Santos, Silvia Helena Rabelo dos
• Banca de defesa: Santos, Silvia Helena Rabelo, Freitas Júnior, Ruffo de, Batista, Aline Carvalho, Celes, Mara Rúbia Nunes, Costa, Nádia do Lago
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Goiás
• Programa de Pós-Graduação: Programa de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP)
• Departamento: Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
• País: Brasil
• Palavras-chave em Português: Câncer de mama; Macrófagos; Biomarcadores; Fatores prognósticos
• Palavras-chave em Inglês: Breast cancer; Macrophages; Biomarkers; Prognostic factors
• Área do conhecimento CNPq: SAUDE COLETIVA::SAUDE PUBLICA
• Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tede/9706

Introduction: The analysis of possible biomarkers associated with the more aggressive behavior of breast carcinomas, linked to clinical and prognostic information, can provide important data in the treatment of patients with breast cancer. There are indications that the presence of tumor associated macrophages (TAMs) in the tumor microenvironment is associated with a worse prognosis for women with breast cancer. Objective: To evaluate the importance of the presence of macrophages in the tumor microenvironment in breast neoplasms. Methods: This retrospective cross-sectional study included 93 cases of women with invasive ductal breast cancer and submitted to immunohistochemical analysis of the markers for CD68 (M1 / M2) and CD163 (M2) macrophages. The counts of CD68 + and CD163 + cells were performed in a peritumoral region, considering hot spot fields, and the cases under study were classified as low infiltration or high infiltration using semiquantitative evaluation of cell marking. Statistical analysis was performed using the Stata 13.0 program (Stata Corp., College Station, TX, United States), with bivariate analysis, considering the level of significance of p <0.05, and multivariate analysis where the variables with p <0.2 were submitted to the logistic regression test. The Kaplan-Meier method and the log-rank test were used in the analysis of survival curves. Results: High levels of TAMs (CD68 +) were significantly correlated with: patient age, presence of distant metastasis, more advanced tumor staging, absence of estrogen receptors (ER) and progesterone (PR), and triple negative breast cancer (TN). The reduction of the overall survival rate in the cases of women with breast cancer studied was significantly associated with high infiltration of TAMs (CD68 +). The high infiltration of TAMs phenotype M2 (CD163 +) was significantly correlated with the independent variables presence of distant metastasis, absence of RE and TN breast cancer. Conclusions: The association of the analysis of TAM biomarkers with clinical and prognostic information showed that the high presence of macrophagic infiltrate has a significant prognostic value, adding information on the relationship between the presence of macrophages in the tumor microenvironment of cases of women with carcinoma and the clinical-pathological characteristics of the tumor. In addition, TAMs appear as potential prognostic markers for breast cancer.