Avaliação da atividade antifúngica e mecanismo de ação de compostos naturais e sintéticos em leveduras do complexo Cryptococcus neoformans
Ano de defesa: | 2014 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Goiás
|
Programa de Pós-Graduação: |
Programa de Pós-graduação em Biologia das Interações PH (IPTSP)
|
Departamento: |
Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.bc.ufg.br/tede/handle/tede/4180 |
Resumo: | Fungal infections caused by yeasts of Cryptococcus neoformans complex have increased considerably among immunosuppressed especially in patients infected with human immunodeficiency virus (HIV). Antifungal drugs available to treat these infections have a wide spectrum of action, but the high costs, side effects, besides the acquisition of resistance of fungi make their limited effectiveness. In these circumstances, the search for new drugs is needed. In the present study, we evaluated the antifungal activity of tamoxifen, bisabolol, indinavir, didanosine, UFMG, Labiocon 241, solasodine, Labiocon 237, Clonazepam, semicarbonado Benzaldehyde, Cardanol, AB 36 and three natural extracts of Psychotria spp, Sclerolobium paniculatum, Hymenaea courbaril and cell viability and mechanism of action of the compound showed antifungal activity of 20 isolates of Cryptococcus neoformans. To evaluate the antifungal activity , we used the in vitro susceptibility to yeast broth microdilution protocol M27 - A3 ( CLSI 2008) and to verify the mechanism of action, flow cytometry was performed with the cellular marker Propidium Iodide ( PI ) and cell viability assay with MTT salt. Among the compounds analyzed, only tamoxifen and olanzapine showed inhibitory action on fungal samples at concentrations 128-256 μg/mL. Analysis by flow cytometry was performed with tamoxifen and showed that the compound did not alter the cell membrane of Cryptococcus neoformans ATCC 28957 , however, the cell viability test showed that tamoxifen was able to inhibit the metabolism of the fungus in different concentrations ranging 4-1024 μg/mL. |
id |
UFG-2_3f87de5a81157f82bd49113da3129a3e |
---|---|
oai_identifier_str |
oai:repositorio.bc.ufg.br:tede/4180 |
network_acronym_str |
UFG-2 |
network_name_str |
Repositório Institucional da UFG |
repository_id_str |
|
spelling |
Fernandes, Orionalda Fatima Lisboahttp://lattes.cnpq.br/9826992750728262Silva, Maria do Rosário RodriguesPaula, José Realino dehttp://lattes.cnpq.br/8450766384125001Silva, Kamila Pereira da2015-02-20T10:55:13Z2014-05-14SILVA, K. P. Avaliação da atividade antifúngica e mecanismo de ação de compostos naturais e sintéticos em leveduras do complexo Cryptococcus neoformans. 2014. 74 f. Dissertação (Mestrado em Biologia das Relações Parasito-Hospedeiro)–Universidade Federal de Goiás, Goiânia, 2014.http://repositorio.bc.ufg.br/tede/handle/tede/4180Fungal infections caused by yeasts of Cryptococcus neoformans complex have increased considerably among immunosuppressed especially in patients infected with human immunodeficiency virus (HIV). Antifungal drugs available to treat these infections have a wide spectrum of action, but the high costs, side effects, besides the acquisition of resistance of fungi make their limited effectiveness. In these circumstances, the search for new drugs is needed. In the present study, we evaluated the antifungal activity of tamoxifen, bisabolol, indinavir, didanosine, UFMG, Labiocon 241, solasodine, Labiocon 237, Clonazepam, semicarbonado Benzaldehyde, Cardanol, AB 36 and three natural extracts of Psychotria spp, Sclerolobium paniculatum, Hymenaea courbaril and cell viability and mechanism of action of the compound showed antifungal activity of 20 isolates of Cryptococcus neoformans. To evaluate the antifungal activity , we used the in vitro susceptibility to yeast broth microdilution protocol M27 - A3 ( CLSI 2008) and to verify the mechanism of action, flow cytometry was performed with the cellular marker Propidium Iodide ( PI ) and cell viability assay with MTT salt. Among the compounds analyzed, only tamoxifen and olanzapine showed inhibitory action on fungal samples at concentrations 128-256 μg/mL. Analysis by flow cytometry was performed with tamoxifen and showed that the compound did not alter the cell membrane of Cryptococcus neoformans ATCC 28957 , however, the cell viability test showed that tamoxifen was able to inhibit the metabolism of the fungus in different concentrations ranging 4-1024 μg/mL.As infecções fúngicas causadas por leveduras do complexo Cryptococcus neoformans tem sido reduzida entre os imunossuprimidos principalmente nos pacientes infectados pelo vírus da imunodeficiência humana (HIV). Os antifúngicos disponíveis para o tratamento destas infecções apresentam um amplo espectro de ação, porém os altos custos, efeitos colaterais, além da aquisição de resistência dos fungos tornam sua eficácia limitada. Nestas circunstâncias, a busca por novos fármacos é necessária. No presente trabalho, foi avaliada a atividade antifúngica de tamoxifeno, bisabolol, indinavir, didanosina, Olanzapina, UFMG, Labiocon 241, Solasodina, Labiocon 237, Clonazepam, Benzaldeído semicarbonado, Cardanol, AB 36 e três extratos naturais obtidos de Psychotria spp, Sclerolobium paniculatum, Hymenaea courbaril. A viabilidade celular e o mecanismo de ação do composto que apresentou ação antifúngica sobre 20 isolados de Cryptococcus neoformans formam determinados. A atividade antifúngica foi realizada utilizando-se o método microdiluição em caldo para leveduras. Para verificar do mecanismo de ação, foi realizada a citometria de fluxo com o marcador celular Iodeto de Propídio (PI), enquanto que o teste da viabilidade celular foi obtido usando-se o sal MTT. Dentre os compostos analisados, apenas o tamoxifeno e olanzapina apresentaram ação inibitória sobre as amostras fúngicas, em concentrações de 128 a 256 μg/mL. Pela análise por citometria de fluxo com o tamoxifeno não se verificou alteração da membrana celular de Cryptococcus neoformans ATCC 28957. Entretanto, o tamoxifeno inibiu o metabolismo do fungo em diferentes concentrações, variando de 4 a 1024 μg/mL verificado pelo teste da viabilidade celular.Submitted by Luanna Matias (lua_matias@yahoo.com.br) on 2015-02-09T11:45:00Z No. of bitstreams: 2 Dissertação - Kamila Pereira da Silva - 2014.pdf: 1336089 bytes, checksum: 2658c99debc59b350392edc7239568d9 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2015-02-20T10:55:13Z (GMT) No. of bitstreams: 2 Dissertação - Kamila Pereira da Silva - 2014.pdf: 1336089 bytes, checksum: 2658c99debc59b350392edc7239568d9 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Made available in DSpace on 2015-02-20T10:55:13Z (GMT). No. of bitstreams: 2 Dissertação - Kamila Pereira da Silva - 2014.pdf: 1336089 bytes, checksum: 2658c99debc59b350392edc7239568d9 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2014-05-14application/pdfhttp://repositorio.bc.ufg.br/tede/retrieve/16942/Disserta%c3%a7%c3%a3o%20-%20Kamila%20Pereira%20da%20Silva%20-%202014.pdf.jpgporUniversidade Federal de GoiásPrograma de Pós-graduação em Biologia das Interações PH (IPTSP)UFGBrasilInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessAntifúngicoCryptococcus neoformansSuscetibilidade in vitroCitometria de fluxoAntifungalIn vitro susceptibilityFlow cytometryCIENCIAS BIOLOGICAS::PARASITOLOGIAAvaliação da atividade antifúngica e mecanismo de ação de compostos naturais e sintéticos em leveduras do complexo Cryptococcus neoformansEvaluation of antifungal activity and mechanism of action of natural and synthetic compounds in yeast Cryptococcus neoformans complexinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis6367159433410056917600600600-7769011444564556288-4544576747271574306reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://repositorio.bc.ufg.br/tede/bitstreams/586f5232-2758-4f88-b476-764fa9ff3f2e/downloadbd3efa91386c1718a7f26a329fdcb468MD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://repositorio.bc.ufg.br/tede/bitstreams/c39a8f62-6d1a-41b2-be33-ed083cf9437c/download4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-822901http://repositorio.bc.ufg.br/tede/bitstreams/f69d6629-0194-49f1-9bd6-389c343d9917/download29b9d5e95be03707f9d4a2e110421c11MD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-823148http://repositorio.bc.ufg.br/tede/bitstreams/e66f3490-d10c-44ce-b940-9ab0d254020d/download9da0b6dfac957114c6a7714714b86306MD54ORIGINALDissertação - Kamila Pereira da Silva - 2014.pdfDissertação - Kamila Pereira da Silva - 2014.pdfapplication/pdf1336089http://repositorio.bc.ufg.br/tede/bitstreams/d7716bc8-d704-4e7b-8161-7e9743133d5d/download2658c99debc59b350392edc7239568d9MD55TEXTDissertação - Kamila Pereira da Silva - 2014.pdf.txtDissertação - Kamila Pereira da Silva - 2014.pdf.txtExtracted Texttext/plain114780http://repositorio.bc.ufg.br/tede/bitstreams/bc1dac92-fc5f-46fc-a863-e48cce8aba4b/downloadc4bac65c81472994c5eda5c3cd137760MD56THUMBNAILDissertação - Kamila Pereira da Silva - 2014.pdf.jpgDissertação - Kamila Pereira da Silva - 2014.pdf.jpgGenerated Thumbnailimage/jpeg2420http://repositorio.bc.ufg.br/tede/bitstreams/eca027b4-cba3-455a-9447-d5143b781951/download1ccad6033ff7882b6e198a6d473c53e6MD57tede/41802015-03-05 10:55:00.045http://creativecommons.org/licenses/by-nc-nd/4.0/Acesso Abertoopen.accessoai:repositorio.bc.ufg.br:tede/4180http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttp://repositorio.bc.ufg.br/oai/requesttasesdissertacoes.bc@ufg.bropendoar:2015-03-05T13:55Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)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 |
dc.title.eng.fl_str_mv |
Avaliação da atividade antifúngica e mecanismo de ação de compostos naturais e sintéticos em leveduras do complexo Cryptococcus neoformans |
dc.title.alternative.eng.fl_str_mv |
Evaluation of antifungal activity and mechanism of action of natural and synthetic compounds in yeast Cryptococcus neoformans complex |
title |
Avaliação da atividade antifúngica e mecanismo de ação de compostos naturais e sintéticos em leveduras do complexo Cryptococcus neoformans |
spellingShingle |
Avaliação da atividade antifúngica e mecanismo de ação de compostos naturais e sintéticos em leveduras do complexo Cryptococcus neoformans Silva, Kamila Pereira da Antifúngico Cryptococcus neoformans Suscetibilidade in vitro Citometria de fluxo Antifungal In vitro susceptibility Flow cytometry CIENCIAS BIOLOGICAS::PARASITOLOGIA |
title_short |
Avaliação da atividade antifúngica e mecanismo de ação de compostos naturais e sintéticos em leveduras do complexo Cryptococcus neoformans |
title_full |
Avaliação da atividade antifúngica e mecanismo de ação de compostos naturais e sintéticos em leveduras do complexo Cryptococcus neoformans |
title_fullStr |
Avaliação da atividade antifúngica e mecanismo de ação de compostos naturais e sintéticos em leveduras do complexo Cryptococcus neoformans |
title_full_unstemmed |
Avaliação da atividade antifúngica e mecanismo de ação de compostos naturais e sintéticos em leveduras do complexo Cryptococcus neoformans |
title_sort |
Avaliação da atividade antifúngica e mecanismo de ação de compostos naturais e sintéticos em leveduras do complexo Cryptococcus neoformans |
author |
Silva, Kamila Pereira da |
author_facet |
Silva, Kamila Pereira da |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Fernandes, Orionalda Fatima Lisboa |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/9826992750728262 |
dc.contributor.referee1.fl_str_mv |
Silva, Maria do Rosário Rodrigues |
dc.contributor.referee2.fl_str_mv |
Paula, José Realino de |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/8450766384125001 |
dc.contributor.author.fl_str_mv |
Silva, Kamila Pereira da |
contributor_str_mv |
Fernandes, Orionalda Fatima Lisboa Silva, Maria do Rosário Rodrigues Paula, José Realino de |
dc.subject.por.fl_str_mv |
Antifúngico Cryptococcus neoformans Suscetibilidade in vitro Citometria de fluxo |
topic |
Antifúngico Cryptococcus neoformans Suscetibilidade in vitro Citometria de fluxo Antifungal In vitro susceptibility Flow cytometry CIENCIAS BIOLOGICAS::PARASITOLOGIA |
dc.subject.eng.fl_str_mv |
Antifungal In vitro susceptibility Flow cytometry |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::PARASITOLOGIA |
description |
Fungal infections caused by yeasts of Cryptococcus neoformans complex have increased considerably among immunosuppressed especially in patients infected with human immunodeficiency virus (HIV). Antifungal drugs available to treat these infections have a wide spectrum of action, but the high costs, side effects, besides the acquisition of resistance of fungi make their limited effectiveness. In these circumstances, the search for new drugs is needed. In the present study, we evaluated the antifungal activity of tamoxifen, bisabolol, indinavir, didanosine, UFMG, Labiocon 241, solasodine, Labiocon 237, Clonazepam, semicarbonado Benzaldehyde, Cardanol, AB 36 and three natural extracts of Psychotria spp, Sclerolobium paniculatum, Hymenaea courbaril and cell viability and mechanism of action of the compound showed antifungal activity of 20 isolates of Cryptococcus neoformans. To evaluate the antifungal activity , we used the in vitro susceptibility to yeast broth microdilution protocol M27 - A3 ( CLSI 2008) and to verify the mechanism of action, flow cytometry was performed with the cellular marker Propidium Iodide ( PI ) and cell viability assay with MTT salt. Among the compounds analyzed, only tamoxifen and olanzapine showed inhibitory action on fungal samples at concentrations 128-256 μg/mL. Analysis by flow cytometry was performed with tamoxifen and showed that the compound did not alter the cell membrane of Cryptococcus neoformans ATCC 28957 , however, the cell viability test showed that tamoxifen was able to inhibit the metabolism of the fungus in different concentrations ranging 4-1024 μg/mL. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-05-14 |
dc.date.accessioned.fl_str_mv |
2015-02-20T10:55:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA, K. P. Avaliação da atividade antifúngica e mecanismo de ação de compostos naturais e sintéticos em leveduras do complexo Cryptococcus neoformans. 2014. 74 f. Dissertação (Mestrado em Biologia das Relações Parasito-Hospedeiro)–Universidade Federal de Goiás, Goiânia, 2014. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/4180 |
identifier_str_mv |
SILVA, K. P. Avaliação da atividade antifúngica e mecanismo de ação de compostos naturais e sintéticos em leveduras do complexo Cryptococcus neoformans. 2014. 74 f. Dissertação (Mestrado em Biologia das Relações Parasito-Hospedeiro)–Universidade Federal de Goiás, Goiânia, 2014. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/4180 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
6367159433410056917 |
dc.relation.confidence.fl_str_mv |
600 600 600 |
dc.relation.department.fl_str_mv |
-7769011444564556288 |
dc.relation.cnpq.fl_str_mv |
-4544576747271574306 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Biologia das Interações PH (IPTSP) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFG instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Repositório Institucional da UFG |
collection |
Repositório Institucional da UFG |
bitstream.url.fl_str_mv |
http://repositorio.bc.ufg.br/tede/bitstreams/586f5232-2758-4f88-b476-764fa9ff3f2e/download http://repositorio.bc.ufg.br/tede/bitstreams/c39a8f62-6d1a-41b2-be33-ed083cf9437c/download http://repositorio.bc.ufg.br/tede/bitstreams/f69d6629-0194-49f1-9bd6-389c343d9917/download http://repositorio.bc.ufg.br/tede/bitstreams/e66f3490-d10c-44ce-b940-9ab0d254020d/download http://repositorio.bc.ufg.br/tede/bitstreams/d7716bc8-d704-4e7b-8161-7e9743133d5d/download http://repositorio.bc.ufg.br/tede/bitstreams/bc1dac92-fc5f-46fc-a863-e48cce8aba4b/download http://repositorio.bc.ufg.br/tede/bitstreams/eca027b4-cba3-455a-9447-d5143b781951/download |
bitstream.checksum.fl_str_mv |
bd3efa91386c1718a7f26a329fdcb468 4afdbb8c545fd630ea7db775da747b2f 29b9d5e95be03707f9d4a2e110421c11 9da0b6dfac957114c6a7714714b86306 2658c99debc59b350392edc7239568d9 c4bac65c81472994c5eda5c3cd137760 1ccad6033ff7882b6e198a6d473c53e6 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFG - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
tasesdissertacoes.bc@ufg.br |
_version_ |
1793965711226306560 |