Caracterização filogenética de isolados do vírus dengue em Goiânia, Goiás
| Ano de defesa: | 2015 |
|---|---|
| Autor(a) principal: |
Cunha, Marielton dos Passos
 |
| Orientador(a): |
Fiaccadori, Fabíola Souza
|
| Banca de defesa: | , , |
| Tipo de documento: | Dissertação |
| Tipo de acesso: | Acesso aberto |
| Idioma: | por |
| Instituição de defesa: |
Universidade Federal de Goiás
|
| Programa de Pós-Graduação: |
Programa de Pós-graduação em Biologia das Interações PH (IPTSP)
|
| Departamento: |
Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
|
| País: |
Brasil
|
| Palavras-chave em Português: | |
| Palavras-chave em Inglês: | |
| Área do conhecimento CNPq: | |
| Link de acesso: | http://repositorio.bc.ufg.br/tede/handle/tede/5162 |
Resumo: | Dengue viruses (DENV) serotypes 1, 2, 3, and 4 have been causing yearly outbreaks in Brazil. Nevertheless, the population structure of the viruses transmitted in Goiás state is not well understood. In this study, we investigated the phylogenetic pattern of DENV samples identified in Goiânia, Goiás, Brazil during the 2012/2013 epidemic. Therefore, the entire region of the gene of the envelope (E) protein (1485bp) of 16 DENV-1 samples as well as partial region of this gene (363bp) of seven DENV-4 samples were sequenced. Phylogenetic analysis showed that DENV-1 belongs to the genotype V and presents itself divided into two clades, suggesting co-circulation of two distinct lineages in this region. Still, the molecular analyzes indicated a significant change in the amino acid level E348 position. For DENV-4, the sequences were segregate in a monophyletic group and are classified as genotype II American subclade. The molecular and phylogenetic analysis showed that the region suffered multiple introductions by dengue virus. This is the first report of co-circulation of two lineages of DENV-1, and the circulation of DENV-4 in Goiás state. |
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Fiaccadori, Fabíola Souzahttp://lattes.cnpq.br/0993842409303174Fiaccadori, Fabíola SouzaCoelho, Alexandre Siqueira GuedesCardoso, Divina das Dôres de Paulahttp://lattes.cnpq.br/8889654250506994Cunha, Marielton dos Passos2016-01-29T11:01:41Z2015-04-17CUNHA, M. P. Caracterização filogenética de isolados do vírus dengue em Goiânia, Goiás. 2015. 84 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2015.http://repositorio.bc.ufg.br/tede/handle/tede/5162Dengue viruses (DENV) serotypes 1, 2, 3, and 4 have been causing yearly outbreaks in Brazil. Nevertheless, the population structure of the viruses transmitted in Goiás state is not well understood. In this study, we investigated the phylogenetic pattern of DENV samples identified in Goiânia, Goiás, Brazil during the 2012/2013 epidemic. Therefore, the entire region of the gene of the envelope (E) protein (1485bp) of 16 DENV-1 samples as well as partial region of this gene (363bp) of seven DENV-4 samples were sequenced. Phylogenetic analysis showed that DENV-1 belongs to the genotype V and presents itself divided into two clades, suggesting co-circulation of two distinct lineages in this region. Still, the molecular analyzes indicated a significant change in the amino acid level E348 position. For DENV-4, the sequences were segregate in a monophyletic group and are classified as genotype II American subclade. The molecular and phylogenetic analysis showed that the region suffered multiple introductions by dengue virus. This is the first report of co-circulation of two lineages of DENV-1, and the circulation of DENV-4 in Goiás state.Os vírus dengue (DENV) sorotipos 1, 2, 3 e 4 tem causado surtos anuais no Brasil. No entanto, a estrutura populacional dos vírus transmitidos em Goiás não é bem compreendida. Neste estudo, investigamos o padrão filogenético de amostras do DENV identificadas em Goiânia, Goiás, Brasil, durante a epidemia de 2012/2013. Para isso, a região completa do gene codificante da proteína do envelope (E) (1485pb) de 16 amostras DENV-1 assim como a região parcial deste gene (363pb) de sete amostras DENV-4, foram sequenciadas. A análise filogenética mostrou que o DENV-1 pertence ao genótipo V e apresenta-se dividido em dois clados, sugerindo a cocirculação de duas linhagens distintas na região. Ainda, as análises moleculares indicaram uma alteração significativa na posição do aminoácido E348. Para DENV-4, as sequências segregaram em um grupo monofilético, sendo classificadas como genótipo II subclado americano. As análises moleculares e filogenéticas indicaram que a região sofreu múltiplas introduções pelo DENV. Este é o primeiro relato de cocirculação de duas linhagens de DENV-1, assim como circulação do DENV-4 no estado de Goiás.Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2016-01-29T10:59:58Z No. of bitstreams: 2 Dissertação - Marielton dos Passos Cunha - 2015.pdf: 3794305 bytes, checksum: 63dd946c15b0a01f4a247e2bd60fa1e2 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2016-01-29T11:01:41Z (GMT) No. of bitstreams: 2 Dissertação - Marielton dos Passos Cunha - 2015.pdf: 3794305 bytes, checksum: 63dd946c15b0a01f4a247e2bd60fa1e2 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Made available in DSpace on 2016-01-29T11:01:41Z (GMT). No. of bitstreams: 2 Dissertação - Marielton dos Passos Cunha - 2015.pdf: 3794305 bytes, checksum: 63dd946c15b0a01f4a247e2bd60fa1e2 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2015-04-17Fundação de Amparo à Pesquisa do Estado de Goiás - FAPEGapplication/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Biologia das Interações PH (IPTSP)UFGBrasilInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessDengueLinhagensAnálise filogenéticaDengue virusLineagesPhylogenetic analysisCIENCIAS BIOLOGICAS::PARASITOLOGIACaracterização filogenética de isolados do vírus dengue em Goiânia, GoiásPhylogenetic characterization of isolates of dengue virus in Goiânia, Goiásinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis6367159433410056917600600600600-7769011444564556288-4544576747271574306-961409807440757778reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; 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| dc.title.por.fl_str_mv |
Caracterização filogenética de isolados do vírus dengue em Goiânia, Goiás |
| dc.title.alternative.eng.fl_str_mv |
Phylogenetic characterization of isolates of dengue virus in Goiânia, Goiás |
| title |
Caracterização filogenética de isolados do vírus dengue em Goiânia, Goiás |
| spellingShingle |
Caracterização filogenética de isolados do vírus dengue em Goiânia, Goiás Cunha, Marielton dos Passos Dengue Linhagens Análise filogenética Dengue virus Lineages Phylogenetic analysis CIENCIAS BIOLOGICAS::PARASITOLOGIA |
| title_short |
Caracterização filogenética de isolados do vírus dengue em Goiânia, Goiás |
| title_full |
Caracterização filogenética de isolados do vírus dengue em Goiânia, Goiás |
| title_fullStr |
Caracterização filogenética de isolados do vírus dengue em Goiânia, Goiás |
| title_full_unstemmed |
Caracterização filogenética de isolados do vírus dengue em Goiânia, Goiás |
| title_sort |
Caracterização filogenética de isolados do vírus dengue em Goiânia, Goiás |
| author |
Cunha, Marielton dos Passos |
| author_facet |
Cunha, Marielton dos Passos |
| author_role |
author |
| dc.contributor.advisor1.fl_str_mv |
Fiaccadori, Fabíola Souza |
| dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/0993842409303174 |
| dc.contributor.referee1.fl_str_mv |
Fiaccadori, Fabíola Souza |
| dc.contributor.referee2.fl_str_mv |
Coelho, Alexandre Siqueira Guedes |
| dc.contributor.referee3.fl_str_mv |
Cardoso, Divina das Dôres de Paula |
| dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/8889654250506994 |
| dc.contributor.author.fl_str_mv |
Cunha, Marielton dos Passos |
| contributor_str_mv |
Fiaccadori, Fabíola Souza Fiaccadori, Fabíola Souza Coelho, Alexandre Siqueira Guedes Cardoso, Divina das Dôres de Paula |
| dc.subject.por.fl_str_mv |
Dengue Linhagens Análise filogenética |
| topic |
Dengue Linhagens Análise filogenética Dengue virus Lineages Phylogenetic analysis CIENCIAS BIOLOGICAS::PARASITOLOGIA |
| dc.subject.eng.fl_str_mv |
Dengue virus Lineages Phylogenetic analysis |
| dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::PARASITOLOGIA |
| description |
Dengue viruses (DENV) serotypes 1, 2, 3, and 4 have been causing yearly outbreaks in Brazil. Nevertheless, the population structure of the viruses transmitted in Goiás state is not well understood. In this study, we investigated the phylogenetic pattern of DENV samples identified in Goiânia, Goiás, Brazil during the 2012/2013 epidemic. Therefore, the entire region of the gene of the envelope (E) protein (1485bp) of 16 DENV-1 samples as well as partial region of this gene (363bp) of seven DENV-4 samples were sequenced. Phylogenetic analysis showed that DENV-1 belongs to the genotype V and presents itself divided into two clades, suggesting co-circulation of two distinct lineages in this region. Still, the molecular analyzes indicated a significant change in the amino acid level E348 position. For DENV-4, the sequences were segregate in a monophyletic group and are classified as genotype II American subclade. The molecular and phylogenetic analysis showed that the region suffered multiple introductions by dengue virus. This is the first report of co-circulation of two lineages of DENV-1, and the circulation of DENV-4 in Goiás state. |
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2015 |
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2015-04-17 |
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2016-01-29T11:01:41Z |
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CUNHA, M. P. Caracterização filogenética de isolados do vírus dengue em Goiânia, Goiás. 2015. 84 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2015. |
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http://repositorio.bc.ufg.br/tede/handle/tede/5162 |
| identifier_str_mv |
CUNHA, M. P. Caracterização filogenética de isolados do vírus dengue em Goiânia, Goiás. 2015. 84 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2015. |
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Universidade Federal de Goiás |
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