Biorremediação de azul de indigotina por lacase imobilizada em goma polissacarídeos purificados de Anadenanthera colubrina

• Ano de defesa: 2019
• Autor(a) principal: Bezerra, Alyne Gonçalves
• Orientador(a): Santos, Pierre Alexandre dos
• Banca de defesa: Santos, Pierre Alexandre dos, Fiuza, Tatiana de Sousa, Bara, Maria Teresa Freitas, Garcia, Telma Alves
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Goiás
• Programa de Pós-Graduação: Programa de Pós-graduação em Ciências Farmacêuticas (FF)
• Departamento: Faculdade Farmácia - FF (RG)
• País: Brasil
• Palavras-chave em Português: Química verde; Indutores naturais da lacase; Piptadenia macrocarpa Benth
• Palavras-chave em Inglês: Green chemistry; Natural inducers of laccase; Piptadenia macrocarpa Benth
• Área do conhecimento CNPq: CIENCIAS DA SAUDE::FARMACIA
• Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tede/10711

Lacasses are capable of oxidizing various pollutants, suggesting their potential in the treatment of effluents with dyes. It was tried to produce beads with Anadenanthera colubrina gum and polysaccharides to immobilize the laccase and to use it for the bioremediation of indigotine blue dye. The extraction of polysaccharides from A. colubrina was optimized by factorial planning using as variables the pH, temperature and concentration of the gum. Pycnoporus sanguineus was used to produce laccase being the residue of the bark of Theobroma cacao as the substrate. The beads were optimized to immobilize the laccase produced by varying the concentration of the structural reagents. Immobilized laccase beads were used to bioremediate the indigotin blue dye 10 mg/L, compared to the use of the free enzyme. The optimized extraction condition of the polysaccharides were pH 5.75, temperature 30 ° C with yield of 83,01%. The laccase produced with the T. cacao residue had better enzymatic activity on day 6 of 3,000 U.mL-1. The beads optimized with the immobilized enzyme were the 16P formulation, therefore the purified polysaccharides were better used for the immobilization of the laccase in the beads. The laccase was efficient in bioremediation 94% of the dye, and the beads with immobilized enzyme bioremediated 68%.