Consumo alimentar e expressão do miR-375 de pacientes com carcinoma espinocelular de cabeça e pescoço

• Ano de defesa: 2019
• Autor(a) principal: Ferreira, Tathiany Jéssica
• Orientador(a): Horst, Maria Aderuza
• Banca de defesa: Horst, Maria Aderuza, Saddi, Vera Aparecida, Vilela, Ana Amélia Freitas
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Goiás
• Programa de Pós-Graduação: Programa de Pós-graduação em Nutrição e Saúde (FANUT)
• Departamento: Faculdade de Nutrição - FANUT (RG)
• País: Brasil
• Palavras-chave em Português: Neoplasias de cabeça e pescoço; Carcinoma espinocelular; MicroRNA-375; Consumo alimentar; Nutrientes; Enxágue oral
• Palavras-chave em Inglês: Head and neck neoplasias; Squamous cell carcinoma; Food consumption; Nutrients; Oral rinse
• Área do conhecimento CNPq: CIENCIAS DA SAUDE::NUTRICAO::ANALISE NUTRICIONAL DE POPULACAO
• Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tede/12284

Objective: To determine the association between the miR-375 expression pattern and the dietary intake of patients in pre-treatment head and neck squamous cell carcinoma (HNSCC). Material and methods: This is an observational cross-sectional study type. Were included 67 patients with a diagnosis of (HNSCC), both sexes, from 19 to 80 years. The variables investigated were: age, sex, profession and ethnicity; use of alcohol and tobacco, food consumption; tumor staging and anatomical site. The instrument used to evaluate food intake was the food frequency questionnaire (FFQ) from the ELSA-BRAZIL study. The intake of energy, macronutrients and micronutrients, as well as food groups, were evaluated. The expression of miR-375 was evaluated by reverse transcription followed by real-time polymerase chain reaction (RT-qPCR), in an oral rinse sample. Results: The sample comprised 43.3% of the patients with the neoplasia in the oropharynx cavity; 37.3% in the oral cavity, and 19.4% in the hypopharynx / larynx. Between the patients evaluated by anatomical sites, there was no significant difference between dietary intake of energy, nutrientes, and food groups. Also, there was no difference in miR-375 expression profile between anatomical sites. In addition, the expression of miR-375 was positively associated with the consumption of energy, iron, magnesium, selenium, vitamin B1, vitamin E, and alcohol. However, it was negatively associated with the intake of carbohydrates, cholesterol, phosphorus, and folate. Conclusions: The findings indicate a possible association between feeding and expression of miR-375, considered a tumor suppressor in head and neck neoplasms.