Investigação microbiológica e análise qualitativa de achados bacteriológicos e micológicos em placas de cultivo de embriões em laboratório de reprodução humana

• Ano de defesa: 2010
• Autor(a) principal: RIBEIRO, Barbara Rosa Foizer
• Orientador(a): AMARAL, Waldemar Naves do
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Goiás
• Programa de Pós-Graduação: Mestrado em Ciências da Saúde
• Departamento: Ciências da Saúde - Medicina
• País: BR
• Palavras-chave em Português: Reprodução humana; Contaminação; Microbiológico; Laboratório; Placas de Cultivo; Embriões
• Palavras-chave em Inglês: Reproduction; Microbial; Contamination; Laboratory; Embryos; Culture Dishes
• Área do conhecimento CNPq: CNPQ::CIENCIAS DA SAUDE
• Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tde/1706

Introduction: Laboratories in Human Reproduction, quality control is crucial to the success of procedures. The correct implementation of procedures directly influence the results, especially because the vagina, the follicular fluid and semen can not be sterilized. A high degree of hygiene, cleanliness and disposal of the material must be observed to avoid contamination in the culture media and equipment. The main causes of this contamination has been associated with infection in male and female genital tract and subsequent contamination of oocytes and embryos of patients. Can also come from contamination of air, machinery and materials such as culture dishes. Objectives: of this study were the presence of market info bacteriological contamination and mycological culture dishes of human embryos and to identify the genus level. Methodology: 125 samples were collected from culture dishes human embryos after transfer to the uterus, in three laboratories of human reproduction in the city of Goiania-GO, in the period 2009 to first half of 2010. The culture media were inoculated in BHI broth and incubated in the greenhouse. Samples that clouded (positivist) were isolated and identified. Results: showed a prevalence of 4.8% of contamination and micro-organisms found were Escherichia coli (50%), Klebsiella sp (16.6%), Pseudomonas sp (16.6%), yeast (16.6%). The E. coli bacteria were of the highest incidence was found in three samples. Although the culture media provide the antibiotics penicillin G (IVF) or gentamicin (HTF), resistant Gram-negative rods were found. Conclusion: Results with a prevalence of 4.8% of contamination, and the microorganisms isolated and their amount per sample: Escherichia coli (3), Klebsiella sp (1), Pseudomonas sp (1), yeast (1).