Associações binárias de timol, carvacrol e eugenol para o controle de fases imaturas de vida livre de Amblyomma sculptum

Detalhes bibliográficos
Ano de defesa: 2020
Autor(a) principal: Silva, Francisca Letícia Vale da lattes
Orientador(a): Monteiro, Caio Márcio de Oliveira lattes
Banca de defesa: Monteiro, Caio Márcio de Oliveira, Lopes, Welber Daniel Zanetti, Ferreira, Lorena Lopes
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Goiás
Programa de Pós-Graduação: Programa de Pós-graduação em Ciência Animal (EVZ)
Departamento: Escola de Veterinária e Zootecnia - EVZ (RG)
País: Brasil
Palavras-chave em Português:
Palavras-chave em Inglês:
Área do conhecimento CNPq:
Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tede/10935
Resumo: His study aimed to evaluate whether the associations between thymol, carvacrol and eugenol on the immature life stages of Amblyomma sculptum have a synergistic effect, by means of in vitro test and under semi field conditions, as well as to evaluate the cost-benefit ratio of formulations. To assess the synergism, the substances were tested in vitro in isolation and associated at concentrations of 0.3125; 0.625; 1.25; 2.5; 5.0 and 10.0 mg/mL on non-fed larvae; and 2.5; 3.75; 5.0; 7.5; 10.0; 15.0 and 20 mg/mL on non-fed nymphs. For the test in semi field conditions, the two associations that obtained the best efficacy in in vitro tests were selected for application on plots of Panicum maximum artificially infested with A. sculptum larvae. In in vitro tests with larvae, the association between thymol and eugenol demonstrated synergistic effect at all tested concentrations; treatments associating thymol + carvacrol and eugenol + carvacrol demonstrated synergistic effect at a concentration of 5.0 mg/mL. In in vitro tests with nymphs, the association between thymol + eugenol showed synergistic effect at a concentration of 5.0 mg/mL; the association between thymol + carvacrol resulted in a synergistic effect at a concentration of 10.0 mg/mL while treatments with the association of carvacrol + eugenol showed moderate synergism at concentrations of 5.0; 7.5 and 10.0 mg/mL. The association of the substances allowed lower use to achieve mortality rates higher than 95% in in vitro tests, resulting in cost reduction. In semi field conditions the efficacy of the associations was 63.2% for thymol + eugenol association and 42.6% for carvacrol + eugenol association. Thus, depending on concentration, synergistic effects of binary associations between thymol, carvacrol and eugenol were observed for immature stages of A. sculptum, in addition to allowing cost reduction with the active principle for formulation development.
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spelling Monteiro, Caio Márcio de Oliveirahttp://lattes.cnpq.br/249641930639465Fernandes, Éverton Kort Kamphttp://lattes.cnpq.br/213554173234115Monteiro, Caio Márcio de OliveiraLopes, Welber Daniel ZanettiFerreira, Lorena Lopeshttp://lattes.cnpq.br/3386333475650462Silva, Francisca Letícia Vale da2020-11-17T11:48:25Z2020-11-17T11:48:25Z2020-08-17SILVA, F. L. V. Associações binárias de timol, carvacrol e eugenol para o controle de fases imaturas de vida livre de Amblyomma sculptum. 2020. 71 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2020.http://repositorio.bc.ufg.br/tede/handle/tede/10935His study aimed to evaluate whether the associations between thymol, carvacrol and eugenol on the immature life stages of Amblyomma sculptum have a synergistic effect, by means of in vitro test and under semi field conditions, as well as to evaluate the cost-benefit ratio of formulations. To assess the synergism, the substances were tested in vitro in isolation and associated at concentrations of 0.3125; 0.625; 1.25; 2.5; 5.0 and 10.0 mg/mL on non-fed larvae; and 2.5; 3.75; 5.0; 7.5; 10.0; 15.0 and 20 mg/mL on non-fed nymphs. For the test in semi field conditions, the two associations that obtained the best efficacy in in vitro tests were selected for application on plots of Panicum maximum artificially infested with A. sculptum larvae. In in vitro tests with larvae, the association between thymol and eugenol demonstrated synergistic effect at all tested concentrations; treatments associating thymol + carvacrol and eugenol + carvacrol demonstrated synergistic effect at a concentration of 5.0 mg/mL. In in vitro tests with nymphs, the association between thymol + eugenol showed synergistic effect at a concentration of 5.0 mg/mL; the association between thymol + carvacrol resulted in a synergistic effect at a concentration of 10.0 mg/mL while treatments with the association of carvacrol + eugenol showed moderate synergism at concentrations of 5.0; 7.5 and 10.0 mg/mL. The association of the substances allowed lower use to achieve mortality rates higher than 95% in in vitro tests, resulting in cost reduction. In semi field conditions the efficacy of the associations was 63.2% for thymol + eugenol association and 42.6% for carvacrol + eugenol association. Thus, depending on concentration, synergistic effects of binary associations between thymol, carvacrol and eugenol were observed for immature stages of A. sculptum, in addition to allowing cost reduction with the active principle for formulation development.Este estudo teve como objetivo avaliar se as associações binárias entre timol, carvacrol e eugenol possuem efeito sinérgico sobre os estágios de vida imatura de Amblyomma sculptum. Para isso realizou-se teste in vitro para avaliação da mortalidade de larvas e ninfas e também teste de semi-campo para avaliar a eficácia dessas. Também foi realizada análise custo para produção de uma formulação que cause 95% de mortalidade. As substâncias foram testadas in vitro de forma isolada e associadas nas concentrações de 0,3125; 0,625; 1,25; 2,5; 5,0 e 10,0 mg/mL, sobre larvas não alimentadas; e nas concentrações de 2,5; 3,75; 5,0; 7,5; 10,0; 15,0 e 20 mg/mL, sobre ninfas não alimentadas. Para realização do teste em condições de semi-campo foram selecionadas as duas associações que obtiveram melhor eficácia nos testes in vitro para aplicação em parcelas de Panicum maximum, infestadas artificialmente com larvas de A. sculptum. Nos testes in vitro, com larvas, a associação entre timol e eugenol, demonstrou efeito sinérgico em todas as concentrações testadas; os tratamentos associando timol + carvacrol e eugenol + carvacrol resultou em efeito sinérgico a partir da concentração de 5,0 mg/mL. Nos testes in vitro com ninfas, a associação entre timol + eugenol apresentou efeito sinérgico a partir da concentração de 5,0 mg/mL; a associação entre timol + carvacrol resultou em efeito sinérgico na concentração de 10,0 mg/mL, enquanto nos tratamentos com a associação de carvacrol + eugenol, foi observado sinergismo moderado nas concentrações de 5,0; 7,5 e 10,0 mg/mL. A associação das substâncias possibilitou menor uso para alcançar mortalidade superior a 95% nos testes in vitro, resultando em redução custo. Em condições de semi-campo a eficácia das associações foi de 63,2% para associação timol + eugenol e 42,6% na associação de carvacrol + eugenol. Assim, concluímos que dependendo da concentração, as associações binárias entre timol, carvacrol e eugenol apresentam efeito sinérgico sobre estágios imaturos de A. sculptum, propiciando redução de concentrações e de custo. As associações timol + eugenol e carvacrol + eugenol também foram capazes de reduzir o número de larvas na vegetação.Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2020-11-16T12:22:49Z No. of bitstreams: 2 license_rdf: 805 bytes, checksum: 4460e5956bc1d1639be9ae6146a50347 (MD5) Dissertação - Francisca Letícia Vale da Silva - 2020.pdf: 2386784 bytes, checksum: 3fbe37cc16dc062530cb8dd51dcfd110 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2020-11-17T11:48:25Z (GMT) No. of bitstreams: 2 license_rdf: 805 bytes, checksum: 4460e5956bc1d1639be9ae6146a50347 (MD5) Dissertação - Francisca Letícia Vale da Silva - 2020.pdf: 2386784 bytes, checksum: 3fbe37cc16dc062530cb8dd51dcfd110 (MD5)Made available in DSpace on 2020-11-17T11:48:25Z (GMT). No. of bitstreams: 2 license_rdf: 805 bytes, checksum: 4460e5956bc1d1639be9ae6146a50347 (MD5) Dissertação - Francisca Letícia Vale da Silva - 2020.pdf: 2386784 bytes, checksum: 3fbe37cc16dc062530cb8dd51dcfd110 (MD5) Previous issue date: 2020-08-17porUniversidade Federal de GoiásPrograma de Pós-graduação em Ciência Animal (EVZ)UFGBrasilEscola de Veterinária e Zootecnia - EVZ (RG)Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessComplexo Amblyomma cajennenseÓleos essenciaisCarrapaticidas botânicosMonoterpenoFenilpropanoide Amblyomma cajennense complexBotanical acaricidesEssential oilsMonoterpenePhenylpropanoidCIENCIAS BIOLOGICAS::PARASITOLOGIAAssociações binárias de timol, carvacrol e eugenol para o controle de fases imaturas de vida livre de Amblyomma sculptumBinary combinations of thymol, carvacrol and eugenol for the control of immature phases of free life of Amblyomma sculptuminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis185005005006170reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.bc.ufg.br/tede/bitstreams/dc80fa5b-e76f-4502-8377-3c9e50f3aab3/download8a4605be74aa9ea9d79846c1fba20a33MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.bc.ufg.br/tede/bitstreams/83b87de5-8b33-4368-bc29-012841619368/download4460e5956bc1d1639be9ae6146a50347MD52ORIGINALDissertação - Francisca Letícia Vale da Silva - 2020.pdfDissertação - Francisca Letícia Vale da Silva - 2020.pdfapplication/pdf2386784http://repositorio.bc.ufg.br/tede/bitstreams/779448ea-6147-495b-9360-17d4c2e21f0d/download3fbe37cc16dc062530cb8dd51dcfd110MD53tede/109352020-11-17 08:48:26.287http://creativecommons.org/licenses/by-nc-nd/4.0/Attribution-NonCommercial-NoDerivatives 4.0 Internationalopen.accessoai:repositorio.bc.ufg.br:tede/10935http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttp://repositorio.bc.ufg.br/oai/requesttasesdissertacoes.bc@ufg.bropendoar:2020-11-17T11:48:26Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)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
dc.title.pt_BR.fl_str_mv Associações binárias de timol, carvacrol e eugenol para o controle de fases imaturas de vida livre de Amblyomma sculptum
dc.title.alternative.eng.fl_str_mv Binary combinations of thymol, carvacrol and eugenol for the control of immature phases of free life of Amblyomma sculptum
title Associações binárias de timol, carvacrol e eugenol para o controle de fases imaturas de vida livre de Amblyomma sculptum
spellingShingle Associações binárias de timol, carvacrol e eugenol para o controle de fases imaturas de vida livre de Amblyomma sculptum
Silva, Francisca Letícia Vale da
Complexo Amblyomma cajennense
Óleos essenciais
Carrapaticidas botânicos
Monoterpeno
Fenilpropanoide Amblyomma cajennense complex
Botanical acaricides
Essential oils
Monoterpene
Phenylpropanoid
CIENCIAS BIOLOGICAS::PARASITOLOGIA
title_short Associações binárias de timol, carvacrol e eugenol para o controle de fases imaturas de vida livre de Amblyomma sculptum
title_full Associações binárias de timol, carvacrol e eugenol para o controle de fases imaturas de vida livre de Amblyomma sculptum
title_fullStr Associações binárias de timol, carvacrol e eugenol para o controle de fases imaturas de vida livre de Amblyomma sculptum
title_full_unstemmed Associações binárias de timol, carvacrol e eugenol para o controle de fases imaturas de vida livre de Amblyomma sculptum
title_sort Associações binárias de timol, carvacrol e eugenol para o controle de fases imaturas de vida livre de Amblyomma sculptum
author Silva, Francisca Letícia Vale da
author_facet Silva, Francisca Letícia Vale da
author_role author
dc.contributor.advisor1.fl_str_mv Monteiro, Caio Márcio de Oliveira
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/249641930639465
dc.contributor.advisor-co1.fl_str_mv Fernandes, Éverton Kort Kamp
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/213554173234115
dc.contributor.referee1.fl_str_mv Monteiro, Caio Márcio de Oliveira
dc.contributor.referee2.fl_str_mv Lopes, Welber Daniel Zanetti
dc.contributor.referee3.fl_str_mv Ferreira, Lorena Lopes
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/3386333475650462
dc.contributor.author.fl_str_mv Silva, Francisca Letícia Vale da
contributor_str_mv Monteiro, Caio Márcio de Oliveira
Fernandes, Éverton Kort Kamp
Monteiro, Caio Márcio de Oliveira
Lopes, Welber Daniel Zanetti
Ferreira, Lorena Lopes
dc.subject.por.fl_str_mv Complexo Amblyomma cajennense
Óleos essenciais
Carrapaticidas botânicos
Monoterpeno
topic Complexo Amblyomma cajennense
Óleos essenciais
Carrapaticidas botânicos
Monoterpeno
Fenilpropanoide Amblyomma cajennense complex
Botanical acaricides
Essential oils
Monoterpene
Phenylpropanoid
CIENCIAS BIOLOGICAS::PARASITOLOGIA
dc.subject.eng.fl_str_mv Fenilpropanoide Amblyomma cajennense complex
Botanical acaricides
Essential oils
Monoterpene
Phenylpropanoid
dc.subject.cnpq.fl_str_mv CIENCIAS BIOLOGICAS::PARASITOLOGIA
description His study aimed to evaluate whether the associations between thymol, carvacrol and eugenol on the immature life stages of Amblyomma sculptum have a synergistic effect, by means of in vitro test and under semi field conditions, as well as to evaluate the cost-benefit ratio of formulations. To assess the synergism, the substances were tested in vitro in isolation and associated at concentrations of 0.3125; 0.625; 1.25; 2.5; 5.0 and 10.0 mg/mL on non-fed larvae; and 2.5; 3.75; 5.0; 7.5; 10.0; 15.0 and 20 mg/mL on non-fed nymphs. For the test in semi field conditions, the two associations that obtained the best efficacy in in vitro tests were selected for application on plots of Panicum maximum artificially infested with A. sculptum larvae. In in vitro tests with larvae, the association between thymol and eugenol demonstrated synergistic effect at all tested concentrations; treatments associating thymol + carvacrol and eugenol + carvacrol demonstrated synergistic effect at a concentration of 5.0 mg/mL. In in vitro tests with nymphs, the association between thymol + eugenol showed synergistic effect at a concentration of 5.0 mg/mL; the association between thymol + carvacrol resulted in a synergistic effect at a concentration of 10.0 mg/mL while treatments with the association of carvacrol + eugenol showed moderate synergism at concentrations of 5.0; 7.5 and 10.0 mg/mL. The association of the substances allowed lower use to achieve mortality rates higher than 95% in in vitro tests, resulting in cost reduction. In semi field conditions the efficacy of the associations was 63.2% for thymol + eugenol association and 42.6% for carvacrol + eugenol association. Thus, depending on concentration, synergistic effects of binary associations between thymol, carvacrol and eugenol were observed for immature stages of A. sculptum, in addition to allowing cost reduction with the active principle for formulation development.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-11-17T11:48:25Z
dc.date.available.fl_str_mv 2020-11-17T11:48:25Z
dc.date.issued.fl_str_mv 2020-08-17
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.citation.fl_str_mv SILVA, F. L. V. Associações binárias de timol, carvacrol e eugenol para o controle de fases imaturas de vida livre de Amblyomma sculptum. 2020. 71 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2020.
dc.identifier.uri.fl_str_mv http://repositorio.bc.ufg.br/tede/handle/tede/10935
identifier_str_mv SILVA, F. L. V. Associações binárias de timol, carvacrol e eugenol para o controle de fases imaturas de vida livre de Amblyomma sculptum. 2020. 71 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2020.
url http://repositorio.bc.ufg.br/tede/handle/tede/10935
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dc.publisher.none.fl_str_mv Universidade Federal de Goiás
dc.publisher.program.fl_str_mv Programa de Pós-graduação em Ciência Animal (EVZ)
dc.publisher.initials.fl_str_mv UFG
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Escola de Veterinária e Zootecnia - EVZ (RG)
publisher.none.fl_str_mv Universidade Federal de Goiás
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