Estudo da composição química, de atividades biológicas e microencapsulação do óleo essencial dos frutos de Pterodon emarginatus Vogel - Fabaceae ("sucupira")

• Ano de defesa: 2012
• Autor(a) principal: ALVES, Suzana Ferreira
• Orientador(a): BARA, Maria Teresa Freitas
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Goiás
• Programa de Pós-Graduação: Mestrado em Ciências Farmacêuticas
• Departamento: Ciências da Saúde - Farmácia
• País: BR
• Palavras-chave em Português: Pterodon emarginatus; Variabilidade química; Microencapsulação; Spray drying; Validação analítica; β -cariofileno; 1. Óleos essenciais; 2. Sucupira; 3. Plantas medicinais
• Palavras-chave em Inglês: Pterodon emarginatus; Chemical variability; Microencapsulation; Spray drying; Analytical validation; β -caryophyllene
• Área do conhecimento CNPq: CNPQ::CIENCIAS DA SAUDE::FARMACIA
• Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tde/2112

The present work aimed to do the study of chemical variability, evaluating biological activities and microencapsulate the essential oil of the fruits of Pterodon emarginatus Vogel (Fabaceae). Chapter 1 presents the study of the chemical variability of essential oil from fruits of sucupira of 11 individual of the Brazilian savanna, collected from five different populations, and were identified as its chemical composition by gas chromatography coupled to mass spectrometry (GC/MS), used for data analysis to multivariate statistical tool that indicated compounds β caryophyllene and α copaene as the main discriminant of the samples studied, both of which are of greater significance. Chapter 2 describes the antimicrobial activity and chemical composition of total oil of the fruits of sucupira, four samples being analyzed, three of the city of Jussara GO and one of region of Jaciara MT. The oils tested showed good antimicrobial activity against bacteria Gram positive (Gram (+)) and moderate activity against Enterobacter aerogenes ATCC 13048. Chapter 3 describes the process of obtaining microcapsules containg essential oil (OE) of sucupira employing the technique of spray drying and the development and validation methodology for quantification of the compound β caryophyllene. Were used gum Arabic and maltodextrin as wall material and prepared five different dispersions (Emulsion 1-E1, Emulsion 2-E2, Emulsion 3-E3, Emulsion 4-E4 and Emulsion 5-E5) which then were atomized in spray dryer. The results show that the drying condition most appropriate was sprinkler beak of 1,2 mm of diameter, power flow the emulsion in system of 4 mL/min, inlet temperature of 160ºC, air flow of 40 L/min e and pressure of 60 psi. Among the emulsions, E2 was standardized with adequate proportion of essential oil and wall materials, by presenting microcapsules (MOE) for having thick-walled, with a pronounced retention of essential oil, spherical morphology and low hygroscopicity. The method developed and validated proved to be linear, precise, acuurate and robust. Chapter 4 presents the evaluation of biological activities of antimicrobial essential oil of PES-01 employing the technique of broth microdilution and antinociceptive and anti-inflamatory activities performed to the microcapsules with the essential oil. The models of pain induced by formalin and hot plate were used to assess the antinociceptive activity and the model of carrageenan-induced pleurisy and Evans blue for evaluation of anti-inflamatory activity. The OE has weak antimicrobial activity (500 μg mL-1) against bacteria Gram (+) and against the fungi of the genus Cryptococcus was inactive against S. aureus ATCC 25923, against bacteria Gram (-) and against the fungi of the genus Candida. OE reduced by 61,54% (300 mg kg-¹) of the reactivity to pain. The MOE reduced 40,87% (1,0 g kg-¹) and 41,57% (2,0 g kg-¹) in first phase of the test formalin-induced pain, suggesting, anti-nociceptive activity. The 2nd phase of the test, the essential oil inhibited 52,35% of the pain related to inflammatory mediators and the microcapsules decreased from 25,86% - 55,60% of the pain. MOE at a dose of 1,0 g kg-¹ showed anti-nociceptive activity in hot plate model, suggestion central analgesic activity. In the arrageenaninduced pleurisy the MOE reduced 25,44% of the complete migration of leukocytes and significantly decreased the concentration of Evans blue in 24,18%, which demonstrates important anti-inflamatory activity.