Efeito de vacinas alopática e homeopática frente a Mycobacterium spp em diferentes modelos animais

Detalhes bibliográficos
Ano de defesa: 2013
Autor(a) principal: Cavalcanti, Marcos Antônio Rocha lattes
Orientador(a): Junqueira-Kipnis, Ana Paula lattes
Banca de defesa: Junqueira-Kipnis, Ana Paula, Alves Jr, José Roberto Ferreira, Resi, Michelle Guerreiro dos, Fioravanti, Maria Clorinda Soares, Martins, Márcio Eduardo Pereira
Tipo de documento: Tese
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Goiás
Programa de Pós-Graduação: Programa de Pós-graduação em Ciência Animal (EVZ)
Departamento: Escola de Veterinária e Zootecnia - EVZ (RG)
País: Brasil
Palavras-chave em Português:
Alopatia
Homeopatia
Imunidade vacinal
Vacina recombinate
Palavras-chave em Inglês:
Allopathy
Homeopathy
Immunity vaccine
Recombinant vaccine
Área do conhecimento CNPq:
MEDICINA VETERINARIA PREVENTIVA::SAUDE ANIMAL (PROGRAMAS SANITARIOS)
Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tede/3148
Resumo: The development of new vaccines in the control of various diseases in cattle has been increasing the marketing of animals and animal products. Thus we tested two vaccines, a biotherapy homeopathicvaccine and other recombinantallopathic vaccine,using mycobacteria in their formulations that were subsequently tested in mice and cattle. In order to study the prophylactic effect of homeopathic vaccine and the potency to be used as a vaccine, we used a model of immunizations and infections. To this end, we used mice female of BALB / c lineage which were distributed in 15 groups of three animals each. To assess the possible immune mechanisms involved in homeopathic biotherapy vaccinations we used Mycobacterium massiliense. The biotherapics were prepared from mycobacterial M. massiliense. After infections and immunizations, the animals were euthanized and their livers and spleens were harvested, macerated, homogenized, plated and incubated at 37 ° C for five days. Then we did the counting of colony forming units (CFU) of bacteria recovered from organs and according to the results obtained were selected the potencies11cH and 19cH to be tested as vaccine, because they have shown more homogeneous results. In the animals that were immunized with 19 cHthere were induction of IgG2a class antibodies specific to M. massiliense similar to (0.18 ± 0.07) infections alone (0.19 ± .02). To assess allopathic vaccine protection was used mycobacterium (Mycobacterium smegmatis mc2155 with PLA71/Fusion) in cattle. After allopathic vaccinations, blood was collected and serum was removed for ELISA test. Animals that received the recombinant live vaccine expressing protein of fusion showed greater levels of specific antibodies (p <0.01). This study evaluated the effect ofhomeopathic biotherapy vaccine composed of M. massiliense and allopathicvaccine formulated with M.smegmatis recombinant in different animal models, thus concluding that both vaccines and vaccines homeopathic and allopathic using different kinds of mycobacteria can induce humoral immune response in an animal model.
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spelling Junqueira-Kipnis, Ana Paulahttp://lattes.cnpq.br/1252262903952987Nunes, Romão da CunhaRezende, Cintia Silva Minafra eJunqueira-Kipnis, Ana PaulaAlves Jr, José Roberto FerreiraResi, Michelle Guerreiro dosFioravanti, Maria Clorinda SoaresMartins, Márcio Eduardo Pereirahttp://lattes.cnpq.br/4488627171099521Cavalcanti, Marcos Antônio Rocha2014-09-23T20:48:47Z2013-03-26CAVALCANTI, Marcos Antônio Rocha. Efeito de vacinas alopática e homeopática frente a Mycobacterium spp em diferentes modelos animais. 2013. 74 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2013.http://repositorio.bc.ufg.br/tede/handle/tede/3148The development of new vaccines in the control of various diseases in cattle has been increasing the marketing of animals and animal products. Thus we tested two vaccines, a biotherapy homeopathicvaccine and other recombinantallopathic vaccine,using mycobacteria in their formulations that were subsequently tested in mice and cattle. In order to study the prophylactic effect of homeopathic vaccine and the potency to be used as a vaccine, we used a model of immunizations and infections. To this end, we used mice female of BALB / c lineage which were distributed in 15 groups of three animals each. To assess the possible immune mechanisms involved in homeopathic biotherapy vaccinations we used Mycobacterium massiliense. The biotherapics were prepared from mycobacterial M. massiliense. After infections and immunizations, the animals were euthanized and their livers and spleens were harvested, macerated, homogenized, plated and incubated at 37 ° C for five days. Then we did the counting of colony forming units (CFU) of bacteria recovered from organs and according to the results obtained were selected the potencies11cH and 19cH to be tested as vaccine, because they have shown more homogeneous results. In the animals that were immunized with 19 cHthere were induction of IgG2a class antibodies specific to M. massiliense similar to (0.18 ± 0.07) infections alone (0.19 ± .02). To assess allopathic vaccine protection was used mycobacterium (Mycobacterium smegmatis mc2155 with PLA71/Fusion) in cattle. After allopathic vaccinations, blood was collected and serum was removed for ELISA test. Animals that received the recombinant live vaccine expressing protein of fusion showed greater levels of specific antibodies (p <0.01). This study evaluated the effect ofhomeopathic biotherapy vaccine composed of M. massiliense and allopathicvaccine formulated with M.smegmatis recombinant in different animal models, thus concluding that both vaccines and vaccines homeopathic and allopathic using different kinds of mycobacteria can induce humoral immune response in an animal model.O desenvolvimento de novas vacinas no controle de várias doenças na bovinocultura vem incrementando a comercialização de animais e produtos de origem animal. Com isso testaram-se duas vacinas, uma vacina bioterápica homeopática e outra vacina alopática recombinante, utilizando micobacterias em suas formulações que posteriormente foram testadas em camundongos e bovinos. Com o objetivo de estudar o efeito profilático da vacina homeopática e a potência a ser utilizada como vacina, foi empregado um modelo de imunizações e infecções. Para tal, utilizou-se camundongos fêmeas da linhagem BALB/c as quais foram distribuídas em 15 grupos com três animais cada. Para avaliar os possíveis mecanismos imunológicos envolvidos nas vacinações bioterápicas homeopáticas utilizou-se Mycobacterium massiliense. Os bioterápicos foram preparados a partir de micobactérias M. massiliense. Após as imunizações e infecções, os animais foram eutanaziados e deles colheram-se os fígados e baços, os quais foram macerados, homogeneizados, plaqueados e incubados a 37ºC durante 5 dias. Em seguida, fez-se a contagem de unidades formadoras de colônias (UFC) das bactérias recuperadas dos órgãos e de acordo com os resultados obtidos foram selecionadas as potências 11cH e 19cH para serem testadas como vacina, por apresentarem resultados mais homogêneos. Nos animais imunizados com 19 cH houve indução da produção de anticorpos da classe IgG2a específicos para M. massiliense semelhantes à (0,18 ± 0,07) infecção sozinha (0,19 ± 0,02). Para avaliar a proteção da vacina alopática, foi utilizada a micobactéria (Mycobacterium smegmatis mc2155 com PLA71/Fusão), em bovinos. Após as vacinações alopáticas foi coletado sangue e retirou-se o soro para o teste de ELISA. Animais que receberam a vacina viva recombinante expressando a proteína de fusão apresentaram níveis maiores de anticorpos específicos (p< 0,01). Com este estudo avaliou-se os efeitos da vacina bioterápica homeopática composta de M. massiliense e de vacina alopática formulada com M. smegmatis recombinante em diferentes modelos animais, concluindo assim que tanto as vacinas homeopáticas e vacinas alopáticas usando diferentes tipos de micobactérias podem induzir resposta imune humoral em modelo animal.Submitted by Erika Demachki (erikademachki@gmail.com) on 2014-09-23T20:38:26Z No. of bitstreams: 2 Cavalcanti, Marcos Antonio Rocha - Tese - 2013.pdf: 964919 bytes, checksum: 063182e6e38ba2cb24520dc287625492 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Approved for entry into archive by Jaqueline Silva (jtas29@gmail.com) on 2014-09-23T20:48:47Z (GMT) No. of bitstreams: 2 Cavalcanti, Marcos Antonio Rocha - Tese - 2013.pdf: 964919 bytes, checksum: 063182e6e38ba2cb24520dc287625492 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Made available in DSpace on 2014-09-23T20:48:47Z (GMT). No. of bitstreams: 2 Cavalcanti, Marcos Antonio Rocha - Tese - 2013.pdf: 964919 bytes, checksum: 063182e6e38ba2cb24520dc287625492 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2013-03-26Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfhttp://repositorio.bc.ufg.br/tede/retrieve/8347/Cavalcanti%2c%20Marcos%20Antonio%20Rocha%20-%20Tese%20-%202013.pdf.jpgporUniversidade Federal de GoiásPrograma de Pós-graduação em Ciência Animal (EVZ)UFGBrasilEscola de Veterinária e Zootecnia - EVZ (RG)Almeida, C.M.C.; Júnior Vasconcelos, A.C.; Kipnis, A.; Andrade, A.L.; Junqueira- Kipnis, A.P. Humoral Immune Response of Tuberculosis Patients in Brazil Indicate Recognition of Mycobacterium tuberculosis MPT-51 and GlcB. Clin Vac Immunol. 2008 15 (3): 579- 581. Ameni, G.; Vordermeier, M.; Aseffa, A.; Young, D.B.; Hewinson, R.G. 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Zhang, H.; Peng, P.; Miao, S.; Zhao, Y.; Mao, F.; Wang, L.; Bai, Y.; Xu, Z.; Wei, S.; Shi, C. Recombinant Mycobacterium smegmatis expressing an ESAT6-CFP10 fusion protein induces anti- mycobacterial immune responses and protects against Mycobacterium tuberculosis challenge in mice. Scand. J. 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dc.title.por.fl_str_mv Efeito de vacinas alopática e homeopática frente a Mycobacterium spp em diferentes modelos animais
dc.title.alternative.eng.fl_str_mv Effect of vaccines and homeophatic Allopathic Mycobacterium spp front in different animal models
title Efeito de vacinas alopática e homeopática frente a Mycobacterium spp em diferentes modelos animais
spellingShingle Efeito de vacinas alopática e homeopática frente a Mycobacterium spp em diferentes modelos animais
Cavalcanti, Marcos Antônio Rocha
Alopatia
Homeopatia
Imunidade vacinal
Vacina recombinate
Allopathy
Homeopathy
Immunity vaccine
Recombinant vaccine
MEDICINA VETERINARIA PREVENTIVA::SAUDE ANIMAL (PROGRAMAS SANITARIOS)
title_short Efeito de vacinas alopática e homeopática frente a Mycobacterium spp em diferentes modelos animais
title_full Efeito de vacinas alopática e homeopática frente a Mycobacterium spp em diferentes modelos animais
title_fullStr Efeito de vacinas alopática e homeopática frente a Mycobacterium spp em diferentes modelos animais
title_full_unstemmed Efeito de vacinas alopática e homeopática frente a Mycobacterium spp em diferentes modelos animais
title_sort Efeito de vacinas alopática e homeopática frente a Mycobacterium spp em diferentes modelos animais
author Cavalcanti, Marcos Antônio Rocha
author_facet Cavalcanti, Marcos Antônio Rocha
author_role author
dc.contributor.advisor1.fl_str_mv Junqueira-Kipnis, Ana Paula
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/1252262903952987
dc.contributor.advisor-co1.fl_str_mv Nunes, Romão da Cunha
dc.contributor.advisor-co2.fl_str_mv Rezende, Cintia Silva Minafra e
dc.contributor.referee1.fl_str_mv Junqueira-Kipnis, Ana Paula
dc.contributor.referee2.fl_str_mv Alves Jr, José Roberto Ferreira
dc.contributor.referee3.fl_str_mv Resi, Michelle Guerreiro dos
dc.contributor.referee4.fl_str_mv Fioravanti, Maria Clorinda Soares
dc.contributor.referee5.fl_str_mv Martins, Márcio Eduardo Pereira
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/4488627171099521
dc.contributor.author.fl_str_mv Cavalcanti, Marcos Antônio Rocha
contributor_str_mv Junqueira-Kipnis, Ana Paula
Nunes, Romão da Cunha
Rezende, Cintia Silva Minafra e
Junqueira-Kipnis, Ana Paula
Alves Jr, José Roberto Ferreira
Resi, Michelle Guerreiro dos
Fioravanti, Maria Clorinda Soares
Martins, Márcio Eduardo Pereira
dc.subject.por.fl_str_mv Alopatia
Homeopatia
Imunidade vacinal
Vacina recombinate
topic Alopatia
Homeopatia
Imunidade vacinal
Vacina recombinate
Allopathy
Homeopathy
Immunity vaccine
Recombinant vaccine
MEDICINA VETERINARIA PREVENTIVA::SAUDE ANIMAL (PROGRAMAS SANITARIOS)
dc.subject.eng.fl_str_mv Allopathy
Homeopathy
Immunity vaccine
Recombinant vaccine
dc.subject.cnpq.fl_str_mv MEDICINA VETERINARIA PREVENTIVA::SAUDE ANIMAL (PROGRAMAS SANITARIOS)
description The development of new vaccines in the control of various diseases in cattle has been increasing the marketing of animals and animal products. Thus we tested two vaccines, a biotherapy homeopathicvaccine and other recombinantallopathic vaccine,using mycobacteria in their formulations that were subsequently tested in mice and cattle. In order to study the prophylactic effect of homeopathic vaccine and the potency to be used as a vaccine, we used a model of immunizations and infections. To this end, we used mice female of BALB / c lineage which were distributed in 15 groups of three animals each. To assess the possible immune mechanisms involved in homeopathic biotherapy vaccinations we used Mycobacterium massiliense. The biotherapics were prepared from mycobacterial M. massiliense. After infections and immunizations, the animals were euthanized and their livers and spleens were harvested, macerated, homogenized, plated and incubated at 37 ° C for five days. Then we did the counting of colony forming units (CFU) of bacteria recovered from organs and according to the results obtained were selected the potencies11cH and 19cH to be tested as vaccine, because they have shown more homogeneous results. In the animals that were immunized with 19 cHthere were induction of IgG2a class antibodies specific to M. massiliense similar to (0.18 ± 0.07) infections alone (0.19 ± .02). To assess allopathic vaccine protection was used mycobacterium (Mycobacterium smegmatis mc2155 with PLA71/Fusion) in cattle. After allopathic vaccinations, blood was collected and serum was removed for ELISA test. Animals that received the recombinant live vaccine expressing protein of fusion showed greater levels of specific antibodies (p <0.01). This study evaluated the effect ofhomeopathic biotherapy vaccine composed of M. massiliense and allopathicvaccine formulated with M.smegmatis recombinant in different animal models, thus concluding that both vaccines and vaccines homeopathic and allopathic using different kinds of mycobacteria can induce humoral immune response in an animal model.
publishDate 2013
dc.date.issued.fl_str_mv 2013-03-26
dc.date.accessioned.fl_str_mv 2014-09-23T20:48:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv CAVALCANTI, Marcos Antônio Rocha. Efeito de vacinas alopática e homeopática frente a Mycobacterium spp em diferentes modelos animais. 2013. 74 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2013.
dc.identifier.uri.fl_str_mv http://repositorio.bc.ufg.br/tede/handle/tede/3148
identifier_str_mv CAVALCANTI, Marcos Antônio Rocha. Efeito de vacinas alopática e homeopática frente a Mycobacterium spp em diferentes modelos animais. 2013. 74 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2013.
url http://repositorio.bc.ufg.br/tede/handle/tede/3148
dc.language.iso.fl_str_mv por
language por
dc.relation.program.fl_str_mv 4581960685150189167
dc.relation.confidence.fl_str_mv 600
600
600
600
dc.relation.department.fl_str_mv -6217552114249094582
dc.relation.cnpq.fl_str_mv 6702961356132007056
dc.relation.sponsorship.fl_str_mv 2075167498588264571
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