Efeito vasorelaxante da estrona sobre aorta torácica de ratos: contribuição ao estudo do mecanismo de ação
Ano de defesa: | 2014 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Goiás
|
Programa de Pós-Graduação: |
Programa de Pós-graduação em Biologia (ICB)
|
Departamento: |
Instituto de Ciências Biológicas - ICB (RG)
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.bc.ufg.br/tede/handle/tede/4871 |
Resumo: | Endogenous estrogens have been associated with greater vascular protection in premenopausal women, and the increased risk of cardiovascular diseases in postmenopausal women can be associated to the decrease in plasma estrogen levels. Furthermore recently studies showed that the use of estrogens, like estrone, exhibits remarkable vascular effect when used on isolated arteries, however any investigation was made to elucidate the mechanism of action of this compound. So, the present study was designed to investigate the ability of estrone to induce vascular relaxation and modulate NO-dependent signaling pathway and analyzed the role of estrogens receptor on estrone-mediated vascular relaxation, compared with the effects promoted by 17β-estradiol. 12 week-old male Wistar rats were used to the vascular reactivity, which was performed in an organ bath study for an isometric tension recording. To the experimental protocols, concentration-response curves (0.1 - 100μM) to estrone or 17β - estradiol were performed. The mechanism contributing to estrone-induced effects were determined comparing with the vascular effects induced by 17β - estradiol that have its effect vascular well characterized. It was observed that the vascular relaxation promoted by estrone is dependent on the endothelium and the estrogen receptor. The vasorelaxant effect promoted by estrone was significantly altered in the presence of the inhibitor of PI3K signaling pathway (wortmannin) and the Ca2+-CaM complex inhibitor (calmidazolium), showing the involvement of PI3K/Ca2+-CaM signaling pathways. This study demonstrate that estrone promoted vasorelaxant effect on rat thoracic aortic on endotheliumdependent manner and its effect depends on the estrogen receptors that activate the PI3K pathway and the Ca2+-calmodulin complex which subsequently activates the NO/cGMP pathway. These results contribute to the better understanding of the role of estrone in the conjugated equine estrogen (CEE) which could be associated to the benefits effects of estrogens in the CEE therapy. |
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Ghedini, Paulo Césarhttp://lattes.cnpq.br/5789550234984454Filgueira, Fernando Paranaibahttp://lattes.cnpq.br/5898311625525147Ghedini, Paulo CésarFilgueira, Fernando ParanaibaKhalil, Najeh MaissarLira, Cláudio André Barbosa dehttp://lattes.cnpq.br/2413066343825025Oliveira, Thiago Sardinha de2015-11-12T09:26:51Z2014-09-19OLIVEIRA, T. S. Efeito vasorelaxante da estrona sobre aorta torácica de ratos: contribuição ao estudo do mecanismo de ação. 2014. 52 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Goiás, Goiânia, 2014.http://repositorio.bc.ufg.br/tede/handle/tede/4871Endogenous estrogens have been associated with greater vascular protection in premenopausal women, and the increased risk of cardiovascular diseases in postmenopausal women can be associated to the decrease in plasma estrogen levels. Furthermore recently studies showed that the use of estrogens, like estrone, exhibits remarkable vascular effect when used on isolated arteries, however any investigation was made to elucidate the mechanism of action of this compound. So, the present study was designed to investigate the ability of estrone to induce vascular relaxation and modulate NO-dependent signaling pathway and analyzed the role of estrogens receptor on estrone-mediated vascular relaxation, compared with the effects promoted by 17β-estradiol. 12 week-old male Wistar rats were used to the vascular reactivity, which was performed in an organ bath study for an isometric tension recording. To the experimental protocols, concentration-response curves (0.1 - 100μM) to estrone or 17β - estradiol were performed. The mechanism contributing to estrone-induced effects were determined comparing with the vascular effects induced by 17β - estradiol that have its effect vascular well characterized. It was observed that the vascular relaxation promoted by estrone is dependent on the endothelium and the estrogen receptor. The vasorelaxant effect promoted by estrone was significantly altered in the presence of the inhibitor of PI3K signaling pathway (wortmannin) and the Ca2+-CaM complex inhibitor (calmidazolium), showing the involvement of PI3K/Ca2+-CaM signaling pathways. This study demonstrate that estrone promoted vasorelaxant effect on rat thoracic aortic on endotheliumdependent manner and its effect depends on the estrogen receptors that activate the PI3K pathway and the Ca2+-calmodulin complex which subsequently activates the NO/cGMP pathway. These results contribute to the better understanding of the role of estrone in the conjugated equine estrogen (CEE) which could be associated to the benefits effects of estrogens in the CEE therapy.Os estrogênios endógenos têm sido associados com uma maior proteção do sistema vascular em mulheres na pré-menopausa, uma vez que os riscos de doenças cardiovasculares em mulheres na pós-menopausa são maiores, alterações estas que se devem à diminuição nos níveis plasmáticos de estrogênios. Além disso, estudos recentes mostraram que o uso de estrogênios, como a estrona, apresenta notável efeito vasorelaxante quando avaliado seu efeito em artérias isoladas, no entanto, nenhuma investigação foi realizada para elucidar o mecanismo de acção deste composto. Assim, o presente estudo procurou investigar o efeito da estrona em aorta de ratos, verificando seu efeito em induzir o relaxamento vascular e modular a via de sinalização dependente do óxido nítrico (NO), e ainda o papel dos receptores de estrogênios, comparando com os efeitos promovidos pelo 17β- estradiol. Os animais utilizados neste estudo foram ratos Wistar com 12 semanas de idade, os quais foram utilizados para realização da reatividade vascular em banho de órgãos isolados. Para os protocolos experimentais, curvas de concentraçãoresposta (0,1-100μM) foram feitas para a estrona ou para o 17β-estradiol e as tensões isométricas gravadas. Os mecanismos envolvidos no efeito induzido pela estrona foram determinados através da incubação de inibidores farmacológicos e comparado ao efeito do 17β-estradiol, que tem seu efeito vascular bem caracterizado. Observou-se que a estrona promove efeito vasorelaxante em aorta torácica de ratos, e que o relaxamento vascular promovido por ela é dependente do endotélio e do receptor de estrogênios. Após ativação do receptor de estrogênios, este ativa as vias de sinalização PI3K e Ca2+-CaM que posteriormente ativam a via NO/GMPc. Estes resultados contribuem para o melhor entendimento do papel da estrona em preparações de estrogênios conjugados equinos (CEE), que pode estar associado aos efeitos de benefícios dos estrogênios na terapia CEE.Submitted by Cláudia Bueno (claudiamoura18@gmail.com) on 2015-11-11T18:59:23Z No. of bitstreams: 2 Dissertação - Thiago Sardinha de Oliveira - 2014.pdf: 1412743 bytes, checksum: d9f93cb6e80442a38393a204842830c9 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2015-11-12T09:26:51Z (GMT) No. of bitstreams: 2 Dissertação - Thiago Sardinha de Oliveira - 2014.pdf: 1412743 bytes, checksum: d9f93cb6e80442a38393a204842830c9 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Made available in DSpace on 2015-11-12T09:26:51Z (GMT). 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dc.title.por.fl_str_mv |
Efeito vasorelaxante da estrona sobre aorta torácica de ratos: contribuição ao estudo do mecanismo de ação |
dc.title.alternative.eng.fl_str_mv |
Vasorelaxant effect of estrone on rat thoracic aorta: contribution to the mechanism of action study |
title |
Efeito vasorelaxante da estrona sobre aorta torácica de ratos: contribuição ao estudo do mecanismo de ação |
spellingShingle |
Efeito vasorelaxante da estrona sobre aorta torácica de ratos: contribuição ao estudo do mecanismo de ação Oliveira, Thiago Sardinha de Estrogênios Estrona Endotélio vascular Óxido nítrico Efeito vasorelaxante Estrogens Estrone Vascular endothelium Nitric oxide Vasorelaxant effect BIOQUIMICA::BIOLOGIA MOLECULAR CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
title_short |
Efeito vasorelaxante da estrona sobre aorta torácica de ratos: contribuição ao estudo do mecanismo de ação |
title_full |
Efeito vasorelaxante da estrona sobre aorta torácica de ratos: contribuição ao estudo do mecanismo de ação |
title_fullStr |
Efeito vasorelaxante da estrona sobre aorta torácica de ratos: contribuição ao estudo do mecanismo de ação |
title_full_unstemmed |
Efeito vasorelaxante da estrona sobre aorta torácica de ratos: contribuição ao estudo do mecanismo de ação |
title_sort |
Efeito vasorelaxante da estrona sobre aorta torácica de ratos: contribuição ao estudo do mecanismo de ação |
author |
Oliveira, Thiago Sardinha de |
author_facet |
Oliveira, Thiago Sardinha de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Ghedini, Paulo César |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/5789550234984454 |
dc.contributor.advisor-co1.fl_str_mv |
Filgueira, Fernando Paranaiba |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/5898311625525147 |
dc.contributor.referee1.fl_str_mv |
Ghedini, Paulo César |
dc.contributor.referee2.fl_str_mv |
Filgueira, Fernando Paranaiba |
dc.contributor.referee3.fl_str_mv |
Khalil, Najeh Maissar |
dc.contributor.referee4.fl_str_mv |
Lira, Cláudio André Barbosa de |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2413066343825025 |
dc.contributor.author.fl_str_mv |
Oliveira, Thiago Sardinha de |
contributor_str_mv |
Ghedini, Paulo César Filgueira, Fernando Paranaiba Ghedini, Paulo César Filgueira, Fernando Paranaiba Khalil, Najeh Maissar Lira, Cláudio André Barbosa de |
dc.subject.por.fl_str_mv |
Estrogênios Estrona Endotélio vascular Óxido nítrico Efeito vasorelaxante |
topic |
Estrogênios Estrona Endotélio vascular Óxido nítrico Efeito vasorelaxante Estrogens Estrone Vascular endothelium Nitric oxide Vasorelaxant effect BIOQUIMICA::BIOLOGIA MOLECULAR CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
dc.subject.eng.fl_str_mv |
Estrogens Estrone Vascular endothelium Nitric oxide Vasorelaxant effect |
dc.subject.cnpq.fl_str_mv |
BIOQUIMICA::BIOLOGIA MOLECULAR CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
description |
Endogenous estrogens have been associated with greater vascular protection in premenopausal women, and the increased risk of cardiovascular diseases in postmenopausal women can be associated to the decrease in plasma estrogen levels. Furthermore recently studies showed that the use of estrogens, like estrone, exhibits remarkable vascular effect when used on isolated arteries, however any investigation was made to elucidate the mechanism of action of this compound. So, the present study was designed to investigate the ability of estrone to induce vascular relaxation and modulate NO-dependent signaling pathway and analyzed the role of estrogens receptor on estrone-mediated vascular relaxation, compared with the effects promoted by 17β-estradiol. 12 week-old male Wistar rats were used to the vascular reactivity, which was performed in an organ bath study for an isometric tension recording. To the experimental protocols, concentration-response curves (0.1 - 100μM) to estrone or 17β - estradiol were performed. The mechanism contributing to estrone-induced effects were determined comparing with the vascular effects induced by 17β - estradiol that have its effect vascular well characterized. It was observed that the vascular relaxation promoted by estrone is dependent on the endothelium and the estrogen receptor. The vasorelaxant effect promoted by estrone was significantly altered in the presence of the inhibitor of PI3K signaling pathway (wortmannin) and the Ca2+-CaM complex inhibitor (calmidazolium), showing the involvement of PI3K/Ca2+-CaM signaling pathways. This study demonstrate that estrone promoted vasorelaxant effect on rat thoracic aortic on endotheliumdependent manner and its effect depends on the estrogen receptors that activate the PI3K pathway and the Ca2+-calmodulin complex which subsequently activates the NO/cGMP pathway. These results contribute to the better understanding of the role of estrone in the conjugated equine estrogen (CEE) which could be associated to the benefits effects of estrogens in the CEE therapy. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-09-19 |
dc.date.accessioned.fl_str_mv |
2015-11-12T09:26:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
OLIVEIRA, T. S. Efeito vasorelaxante da estrona sobre aorta torácica de ratos: contribuição ao estudo do mecanismo de ação. 2014. 52 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Goiás, Goiânia, 2014. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/4871 |
identifier_str_mv |
OLIVEIRA, T. S. Efeito vasorelaxante da estrona sobre aorta torácica de ratos: contribuição ao estudo do mecanismo de ação. 2014. 52 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Goiás, Goiânia, 2014. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/4871 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
6883982777473437920 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 |
dc.relation.department.fl_str_mv |
-3872772117827373404 |
dc.relation.cnpq.fl_str_mv |
3962143990328052072 -1634559385931244697 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Biologia (ICB) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Instituto de Ciências Biológicas - ICB (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFG instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
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UFG |
institution |
UFG |
reponame_str |
Repositório Institucional da UFG |
collection |
Repositório Institucional da UFG |
bitstream.url.fl_str_mv |
http://repositorio.bc.ufg.br/tede/bitstreams/bb5cdcc8-31de-44b4-84d4-1f092adc1a48/download http://repositorio.bc.ufg.br/tede/bitstreams/a4a8644b-1834-440a-857b-51bbc4512d27/download http://repositorio.bc.ufg.br/tede/bitstreams/c5c2b262-141c-4135-b611-d607205c07e0/download http://repositorio.bc.ufg.br/tede/bitstreams/f7c42e70-9e6a-4b7f-af49-d592e6850049/download http://repositorio.bc.ufg.br/tede/bitstreams/7baf5494-9f19-4c02-bda8-c249a56a07ff/download http://repositorio.bc.ufg.br/tede/bitstreams/b341ac22-f161-4b4c-844e-6aa0454c734c/download http://repositorio.bc.ufg.br/tede/bitstreams/6d7252eb-5962-4693-8b0d-e5537601425d/download |
bitstream.checksum.fl_str_mv |
bd3efa91386c1718a7f26a329fdcb468 4afdbb8c545fd630ea7db775da747b2f ae2fe251842ade1134c5d9bb99b6eefe 9da0b6dfac957114c6a7714714b86306 d9f93cb6e80442a38393a204842830c9 5bc09d70ad9f1c50558a748ba9375622 50d6bd347497b0a0b57f514e48708043 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFG - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
tasesdissertacoes.bc@ufg.br |
_version_ |
1798045030726762496 |