Protease monocomponente obtida a partir de Bacillus licheniformis PWD-1 em dietas com diferentes valorizações nutricionaispara frangos de corte

Detalhes bibliográficos
Ano de defesa: 2017
Autor(a) principal: Carneiro, André Luis Machado lattes
Orientador(a): Stringhini, José Henrique lattes
Banca de defesa: Stringhini, José Henrique, Laboissiere, Michele, Leandro, Nadja Susana Mogyca
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Goiás
Programa de Pós-Graduação: Programa de Pós-graduação em Zootecnia (EVZ)
Departamento: Escola de Veterinária e Zootecnia - EVZ (RG)
País: Brasil
Palavras-chave em Português:
Palavras-chave em Inglês:
Área do conhecimento CNPq:
Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tede/7054
Resumo: The objective of this study was to evaluate the effect of adding the protease enzyme 0.05% (Cibenza DP 100) in diets for broilers based on corn and soybean meal with two nutritional matrices. Two experiments were conducted with 5 treatments with a basal corn and soybean meal diet: T1 – basal diet with valued protease matrix twice without the enzyme, T2 – basal diet with valued protease nutritional matrix once without enzyme, T3 – basal diet, T4 – basal diet with valued protease nutritional matrix twice with enzyme and T5 – basal diet with valued protease nutritional matrix once with enzyme. The first experiment was carried out with 1280 chicks Cobb500 strain of 1 to 42 days and the second experiment was carried out with 120 chicks housed in the first experiment, and the chicks were housed in battery cages from 17 to 21 days for digestibility study. The experimental design of the experiments was a randomized block, being in the first experiment the row in the experimental industrial shed the criteria for distribution of the blocks, with 5 treatments and 8 repetitions each containing 32 broilers per box. In the second experiment the floor of the cage was the criteria distribution of the blocks, with 5 treatments and 6 repetitions each containing 4 broilers per cage. The analyzed variables were: digestibility, carcass yield, rest, thighs, wings, abdominal fat and performance variables. In all treatments the data were subjected to analysis of variance, evaluated by the R program, and the means were compared by Tukey test at 10% significance. In the first experiment, it was observed a statistical difference (P <0.10) in the final mean weight, weight gain and feed intake in the pre-initial phase with beneficial results with the enzyme supplementation with valued protease nutritional matrix once. In the period from 1 to 21 days, there was a statistical difference (P <0.10) in feed intake and feed conversion, but with positive results for the control treatment. In the cut yield, there was no statistical difference (P> 0.10) in the most used cuts, similar results between the treatments and the control. Statistically different result (P <0.10) was found with the lowest proportion of abdominal fat in the diet fed with diet supplemented with the enzyme and nutritional matrix valued at one time. In the second experiment, statistical difference (P <0.10) was observed for nitrogen balance, which was positive, resulting in higher retention of nitrogen with lower excretion in the environment. The dry matter retention also showed a statistically different result (P <0.10), with a positive result for the supplementation with the enzyme together with greater metabolizability of the dry matter. Based on this study, the protease enzyme supplementation is recommended along with a valued protease nutritional matrix once, in the pre-initial phase of life of the birds, in order to have a better final weight, better weight gain, lower feed intake, lower proportion of abdominal fat, positive nitrogen balance and higher dry matter retention. Based on this work, it is recommended to supplement the protease enzyme with a valued protease nutritional matrix, especially in the pre-initial phase, in order to have a better final weight, better weight gain, lower feed intake, lower abdominal fat ratio, positive nitrogen balance and higher dry matter retention. In the other stages of life of the birds (initial, growth and final) the enzyme supplementation with a valued protease nutritional matrix was also beneficial, since the results in performance, cut yield and metabolizability were equivalent to control. The treatment diets are reduced in nutritional levels if compared to control diet with ideal dietary level for the broilers, resulting in less expense with the nutrients and maintaining a high yield.
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spelling Stringhini, José Henriquehttp://lattes.cnpq.br/8505634095383289Café, Marcos Barcelloshttp://lattes.cnpq.br/9860968235125158Carvalho, Fabyola Barros dehttp://lattes.cnpq.br/4936451314440305Stringhini, José HenriqueLaboissiere, MicheleLeandro, Nadja Susana Mogycahttp://lattes.cnpq.br/6471995708399791Carneiro, André Luis Machado2017-04-03T11:55:20Z2017-01-25CARNEIRO, A. L. M. Protease monocomponente obtida a partir de Bacillus licheniformis PWD-1 em dietas com diferentes valorizações nutricionaispara frangos de corte. 2017.59 f. Dissertação ( Mestrado em Zootecnia) - Universidade Federal de Goiás, Goiânia, 2017.http://repositorio.bc.ufg.br/tede/handle/tede/7054The objective of this study was to evaluate the effect of adding the protease enzyme 0.05% (Cibenza DP 100) in diets for broilers based on corn and soybean meal with two nutritional matrices. Two experiments were conducted with 5 treatments with a basal corn and soybean meal diet: T1 – basal diet with valued protease matrix twice without the enzyme, T2 – basal diet with valued protease nutritional matrix once without enzyme, T3 – basal diet, T4 – basal diet with valued protease nutritional matrix twice with enzyme and T5 – basal diet with valued protease nutritional matrix once with enzyme. The first experiment was carried out with 1280 chicks Cobb500 strain of 1 to 42 days and the second experiment was carried out with 120 chicks housed in the first experiment, and the chicks were housed in battery cages from 17 to 21 days for digestibility study. The experimental design of the experiments was a randomized block, being in the first experiment the row in the experimental industrial shed the criteria for distribution of the blocks, with 5 treatments and 8 repetitions each containing 32 broilers per box. In the second experiment the floor of the cage was the criteria distribution of the blocks, with 5 treatments and 6 repetitions each containing 4 broilers per cage. The analyzed variables were: digestibility, carcass yield, rest, thighs, wings, abdominal fat and performance variables. In all treatments the data were subjected to analysis of variance, evaluated by the R program, and the means were compared by Tukey test at 10% significance. In the first experiment, it was observed a statistical difference (P <0.10) in the final mean weight, weight gain and feed intake in the pre-initial phase with beneficial results with the enzyme supplementation with valued protease nutritional matrix once. In the period from 1 to 21 days, there was a statistical difference (P <0.10) in feed intake and feed conversion, but with positive results for the control treatment. In the cut yield, there was no statistical difference (P> 0.10) in the most used cuts, similar results between the treatments and the control. Statistically different result (P <0.10) was found with the lowest proportion of abdominal fat in the diet fed with diet supplemented with the enzyme and nutritional matrix valued at one time. In the second experiment, statistical difference (P <0.10) was observed for nitrogen balance, which was positive, resulting in higher retention of nitrogen with lower excretion in the environment. The dry matter retention also showed a statistically different result (P <0.10), with a positive result for the supplementation with the enzyme together with greater metabolizability of the dry matter. Based on this study, the protease enzyme supplementation is recommended along with a valued protease nutritional matrix once, in the pre-initial phase of life of the birds, in order to have a better final weight, better weight gain, lower feed intake, lower proportion of abdominal fat, positive nitrogen balance and higher dry matter retention. Based on this work, it is recommended to supplement the protease enzyme with a valued protease nutritional matrix, especially in the pre-initial phase, in order to have a better final weight, better weight gain, lower feed intake, lower abdominal fat ratio, positive nitrogen balance and higher dry matter retention. In the other stages of life of the birds (initial, growth and final) the enzyme supplementation with a valued protease nutritional matrix was also beneficial, since the results in performance, cut yield and metabolizability were equivalent to control. The treatment diets are reduced in nutritional levels if compared to control diet with ideal dietary level for the broilers, resulting in less expense with the nutrients and maintaining a high yield.Objetivou-se com a presente pesquisa avaliar o efeito da adição da enzima protease 0,05% (Cibenza DP 100) em dietas para frangos de corte a base de milho e farelo de soja. Foram conduzidos dois experimentos com 5 tratamentos cada. Todas as rações foram elaboradas com milho e farelo de soja, diferenciando no seguinte: T1 - ração basal com a matriz da protease valorizada em duas vezes sem a enzima, T2 - ração basal com a matriz da protease valorizada em uma vez sem a enzima, T3 - ração basal, T4 - ração basal com a matriz da protease valorizada em duas vezes com a enzima e T5 – ração basal com a matriz da protease valorizada em uma vez com a enzima. O primeiro experimento foi realizado com 1280 pintos da linhagem Cobb500 de 1 a 42 dias e o segundo com 120 pintos alojados em baterias de gaiolas de 17 a 21 dias para ensaio de digestibilidade. O delineamento experimental foi em blocos casualizados, sendo no experimento 1 a localização da parcela experimental dentro do galpão, com cinco tratamentos e oito repetições cada, contendo 32 aves por box. No experimento 2 o andar da gaiola foi o critério de distribuição dos blocos, com 5 tratamentos e 6 repetições cada, contendo 4 aves por gaiola. As variáveis analisadas nos experimentos 1 e 2 foram: digestibilidade, rendimento de carcaça, peito, coxas, asas, gordura abdominal e variáveis de desempenho. Os dados foram submetidos a analise de variância, avaliados por meio do programa R, e as médias comparadas pelo teste de Tukey a 10% de significância. No primeiro experimento, observou-se diferença estatística (P<0,10) no peso médio final, ganho de peso e consumo de ração na fase pré-inicial com resultados benéficos com a suplementação da enzima com valorização em uma vez da matriz nutricional. Já no período de 1 a 21 dias, houve diferença estatística (P<0,10) no consumo de ração e conversão alimentar, porém com resultados positivos para o tratamento controle. No rendimento de cortes, não houve nenhuma diferença estatística (P>0,10) nos cortes mais utilizados, resultados semelhantes entre os tratamentos e o controle. Resultado diferente estatisticamente (P<0,10) foi encontrado com a menor proporção de gordura abdominal na parcela alimentada com dieta suplementada com a enzima e matriz nutricional valorizada em uma vez. No segundo experimento, observou-se diferença estatística (P<0,10) para balanço de nitrogênio sendo este positivo, resultando em maior retenção de nitrogênio com menor excreção no ambiente. A retenção de matéria seca também mostrou um resultado diferente estatisticamente (P<0,10), com resultado positivo para a suplementação com a enzima juntamente com maior metabolizabilidade da matéria seca. Baseado nesse trabalho recomenda-se a suplementação da enzima protease juntamente com uma valorização da matriz nutricional, principalmente na fase pré-inicial, para que se tenha melhor peso médio final, melhor ganho de peso, menor consumo de ração, menor proporção de gordura abdominal, balanço positivo de nitrogênio e maior retenção de matéria seca. Nas outras fases de vida das aves (inicial, crescimento e final) a suplementação da enzima com a valorização da matriz nutricional também se mostrou benéfica, visto que os resultados em desempenho, rendimento de cortes e metabolizabilidade foram equivalentes ao do tratamento controle. As dietas tratamento são reduzidas em níveis nutricionais se comparadas com a dieta controle com o nível dietético ideal para as aves, isso acaba resultando em menor gasto com os nutrientes e mantendo uma alta produção.Submitted by Erika Demachki (erikademachki@gmail.com) on 2017-03-31T17:47:17Z No. of bitstreams: 2 Dissertação - André Luis Machado Carneiro - 2017.pdf: 930852 bytes, checksum: cf82f8587e94d0d2d10b45c2e242f65c (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2017-04-03T11:55:20Z (GMT) No. of bitstreams: 2 Dissertação - André Luis Machado Carneiro - 2017.pdf: 930852 bytes, checksum: cf82f8587e94d0d2d10b45c2e242f65c (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2017-04-03T11:55:20Z (GMT). No. of bitstreams: 2 Dissertação - André Luis Machado Carneiro - 2017.pdf: 930852 bytes, checksum: cf82f8587e94d0d2d10b45c2e242f65c (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2017-01-25Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Zootecnia (EVZ)UFGBrasilEscola de Veterinária e Zootecnia - EVZ (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessRendimentoEnzimaAviculturaDesempenhoPerformanceYield cutsAvicultureEnzymeZOOTECNIA::PRODUCAO ANIMALProtease monocomponente obtida a partir de Bacillus licheniformis PWD-1 em dietas com diferentes valorizações nutricionaispara frangos de corteMonocomponent protease obtained from Bacillus licheniformis PWD-1 in diets with different nutritional variations for broilersinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-8695879823182088786600600600600-6217552114249094582-75137796603725949922075167498588264571reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; 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dc.title.por.fl_str_mv Protease monocomponente obtida a partir de Bacillus licheniformis PWD-1 em dietas com diferentes valorizações nutricionaispara frangos de corte
dc.title.alternative.eng.fl_str_mv Monocomponent protease obtained from Bacillus licheniformis PWD-1 in diets with different nutritional variations for broilers
title Protease monocomponente obtida a partir de Bacillus licheniformis PWD-1 em dietas com diferentes valorizações nutricionaispara frangos de corte
spellingShingle Protease monocomponente obtida a partir de Bacillus licheniformis PWD-1 em dietas com diferentes valorizações nutricionaispara frangos de corte
Carneiro, André Luis Machado
Rendimento
Enzima
Avicultura
Desempenho
Performance
Yield cuts
Aviculture
Enzyme
ZOOTECNIA::PRODUCAO ANIMAL
title_short Protease monocomponente obtida a partir de Bacillus licheniformis PWD-1 em dietas com diferentes valorizações nutricionaispara frangos de corte
title_full Protease monocomponente obtida a partir de Bacillus licheniformis PWD-1 em dietas com diferentes valorizações nutricionaispara frangos de corte
title_fullStr Protease monocomponente obtida a partir de Bacillus licheniformis PWD-1 em dietas com diferentes valorizações nutricionaispara frangos de corte
title_full_unstemmed Protease monocomponente obtida a partir de Bacillus licheniformis PWD-1 em dietas com diferentes valorizações nutricionaispara frangos de corte
title_sort Protease monocomponente obtida a partir de Bacillus licheniformis PWD-1 em dietas com diferentes valorizações nutricionaispara frangos de corte
author Carneiro, André Luis Machado
author_facet Carneiro, André Luis Machado
author_role author
dc.contributor.advisor1.fl_str_mv Stringhini, José Henrique
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/8505634095383289
dc.contributor.advisor-co1.fl_str_mv Café, Marcos Barcellos
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/9860968235125158
dc.contributor.advisor-co2.fl_str_mv Carvalho, Fabyola Barros de
dc.contributor.advisor-co2Lattes.fl_str_mv http://lattes.cnpq.br/4936451314440305
dc.contributor.referee1.fl_str_mv Stringhini, José Henrique
dc.contributor.referee2.fl_str_mv Laboissiere, Michele
dc.contributor.referee3.fl_str_mv Leandro, Nadja Susana Mogyca
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/6471995708399791
dc.contributor.author.fl_str_mv Carneiro, André Luis Machado
contributor_str_mv Stringhini, José Henrique
Café, Marcos Barcellos
Carvalho, Fabyola Barros de
Stringhini, José Henrique
Laboissiere, Michele
Leandro, Nadja Susana Mogyca
dc.subject.por.fl_str_mv Rendimento
Enzima
Avicultura
Desempenho
topic Rendimento
Enzima
Avicultura
Desempenho
Performance
Yield cuts
Aviculture
Enzyme
ZOOTECNIA::PRODUCAO ANIMAL
dc.subject.eng.fl_str_mv Performance
Yield cuts
Aviculture
Enzyme
dc.subject.cnpq.fl_str_mv ZOOTECNIA::PRODUCAO ANIMAL
description The objective of this study was to evaluate the effect of adding the protease enzyme 0.05% (Cibenza DP 100) in diets for broilers based on corn and soybean meal with two nutritional matrices. Two experiments were conducted with 5 treatments with a basal corn and soybean meal diet: T1 – basal diet with valued protease matrix twice without the enzyme, T2 – basal diet with valued protease nutritional matrix once without enzyme, T3 – basal diet, T4 – basal diet with valued protease nutritional matrix twice with enzyme and T5 – basal diet with valued protease nutritional matrix once with enzyme. The first experiment was carried out with 1280 chicks Cobb500 strain of 1 to 42 days and the second experiment was carried out with 120 chicks housed in the first experiment, and the chicks were housed in battery cages from 17 to 21 days for digestibility study. The experimental design of the experiments was a randomized block, being in the first experiment the row in the experimental industrial shed the criteria for distribution of the blocks, with 5 treatments and 8 repetitions each containing 32 broilers per box. In the second experiment the floor of the cage was the criteria distribution of the blocks, with 5 treatments and 6 repetitions each containing 4 broilers per cage. The analyzed variables were: digestibility, carcass yield, rest, thighs, wings, abdominal fat and performance variables. In all treatments the data were subjected to analysis of variance, evaluated by the R program, and the means were compared by Tukey test at 10% significance. In the first experiment, it was observed a statistical difference (P <0.10) in the final mean weight, weight gain and feed intake in the pre-initial phase with beneficial results with the enzyme supplementation with valued protease nutritional matrix once. In the period from 1 to 21 days, there was a statistical difference (P <0.10) in feed intake and feed conversion, but with positive results for the control treatment. In the cut yield, there was no statistical difference (P> 0.10) in the most used cuts, similar results between the treatments and the control. Statistically different result (P <0.10) was found with the lowest proportion of abdominal fat in the diet fed with diet supplemented with the enzyme and nutritional matrix valued at one time. In the second experiment, statistical difference (P <0.10) was observed for nitrogen balance, which was positive, resulting in higher retention of nitrogen with lower excretion in the environment. The dry matter retention also showed a statistically different result (P <0.10), with a positive result for the supplementation with the enzyme together with greater metabolizability of the dry matter. Based on this study, the protease enzyme supplementation is recommended along with a valued protease nutritional matrix once, in the pre-initial phase of life of the birds, in order to have a better final weight, better weight gain, lower feed intake, lower proportion of abdominal fat, positive nitrogen balance and higher dry matter retention. Based on this work, it is recommended to supplement the protease enzyme with a valued protease nutritional matrix, especially in the pre-initial phase, in order to have a better final weight, better weight gain, lower feed intake, lower abdominal fat ratio, positive nitrogen balance and higher dry matter retention. In the other stages of life of the birds (initial, growth and final) the enzyme supplementation with a valued protease nutritional matrix was also beneficial, since the results in performance, cut yield and metabolizability were equivalent to control. The treatment diets are reduced in nutritional levels if compared to control diet with ideal dietary level for the broilers, resulting in less expense with the nutrients and maintaining a high yield.
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-04-03T11:55:20Z
dc.date.issued.fl_str_mv 2017-01-25
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
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dc.identifier.citation.fl_str_mv CARNEIRO, A. L. M. Protease monocomponente obtida a partir de Bacillus licheniformis PWD-1 em dietas com diferentes valorizações nutricionaispara frangos de corte. 2017.59 f. Dissertação ( Mestrado em Zootecnia) - Universidade Federal de Goiás, Goiânia, 2017.
dc.identifier.uri.fl_str_mv http://repositorio.bc.ufg.br/tede/handle/tede/7054
identifier_str_mv CARNEIRO, A. L. M. Protease monocomponente obtida a partir de Bacillus licheniformis PWD-1 em dietas com diferentes valorizações nutricionaispara frangos de corte. 2017.59 f. Dissertação ( Mestrado em Zootecnia) - Universidade Federal de Goiás, Goiânia, 2017.
url http://repositorio.bc.ufg.br/tede/handle/tede/7054
dc.language.iso.fl_str_mv por
language por
dc.relation.program.fl_str_mv -8695879823182088786
dc.relation.confidence.fl_str_mv 600
600
600
600
dc.relation.department.fl_str_mv -6217552114249094582
dc.relation.cnpq.fl_str_mv -7513779660372594992
dc.relation.sponsorship.fl_str_mv 2075167498588264571
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Universidade Federal de Goiás
dc.publisher.program.fl_str_mv Programa de Pós-graduação em Zootecnia (EVZ)
dc.publisher.initials.fl_str_mv UFG
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Escola de Veterinária e Zootecnia - EVZ (RG)
publisher.none.fl_str_mv Universidade Federal de Goiás
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