Métodos eletroanalíticos e ensaios de atividade antioxidante no controle de qualidade de mel
Ano de defesa: | 2015 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Goiás
|
Programa de Pós-Graduação: |
Programa de Pós-graduação em Ciências Farmacêuticas (FF)
|
Departamento: |
Faculdade Farmácia - FF (RG)
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.bc.ufg.br/tede/handle/tede/4996 |
Resumo: | The honey is a concentrated source of nutrients, it is used by animals and exploited as a natural resource for homes since antiquity. Many studies have tried to characterize the honey and its quality, but the complex natural process of production, involve that any research is not able to obtain fully satisfactory results. In this work 24 samples. Were studied 24 samples from honey producing regions located around Goiania - GO and some foreign samples (Portugual, USA and New Zelândias), those donated by the Beekeeping Association of the State of Goiás and these purchased in specialty stores in their respective countries . The physical and chemical tests as indicative of deterioration, hydroxymethylfurfural (HMF) and diastase activity (AD), followed parameters proposed by Normative Instruction N. 11/2000 of the Ministry of Agriculture, Livestock and Supply (MAPA). The antioxidant components analysis was used for traditional spectroscopic methods Folin-Ciocalteu (FC), Ferric Reducing Antioxidant Potential (FRAP), 2,2-diphenyl-1-picryl-hydrazyl (DPPH●); and electroanalytical calculated from the oxidation peaks voltammetric differential pulse (DPV) by Electrochemical Index (EI). While mid-infrared method (IR) and methods of multivariate statistical analysis were used for identification and classification of honey from different floral sources and/or regions. As for the deterioration analysis test (HMF and AD) 50% of the samples applied in these tests, for the control of physical and chemical quality, were at odds with the Normative Instruction N. 11/2000 of MAPA. All samples showed the presence of antioxidant compounds exposed by methods FC, FRAP, DPPH● and EI; and the method of FRAP was the one closest to the EI and the DPPH● was inversely proportional to EI, which may conclude that the electroanalytical by DPV is suitable for analysis of antioxidant compounds in honey. The infrared results coupled with chemometric tool, principal component analysis (PCA) and hierarchical cluster analysis (HCA), were not satisfactory for sample groups of the same floral origin and/or region, but suggested trend in results. |
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Gil, Eric de Souzahttp://lattes.cnpq.br/3218622824233303Garcia, Telma Alveshttp://lattes.cnpq.br/6340261924930737Gil, Eric de SouzaOliveira, Severino Carlos BezerraSantos, Wallans Torres Pio doshttp://lattes.cnpq.br/8713436663797736Rezende, Stefani Garcia2015-12-04T08:43:59Z2015-03-30REZENDE, S. G. Métodos eletroanalíticos e ensaios de atividade antioxidante no controle de qualidade de mel. 2015. 53 f. Dissertação (Mestrado em Ciências Farmacêuticas) - Universidade Federal de Goiás, Goiânia, 2015.http://repositorio.bc.ufg.br/tede/handle/tede/4996The honey is a concentrated source of nutrients, it is used by animals and exploited as a natural resource for homes since antiquity. Many studies have tried to characterize the honey and its quality, but the complex natural process of production, involve that any research is not able to obtain fully satisfactory results. In this work 24 samples. Were studied 24 samples from honey producing regions located around Goiania - GO and some foreign samples (Portugual, USA and New Zelândias), those donated by the Beekeeping Association of the State of Goiás and these purchased in specialty stores in their respective countries . The physical and chemical tests as indicative of deterioration, hydroxymethylfurfural (HMF) and diastase activity (AD), followed parameters proposed by Normative Instruction N. 11/2000 of the Ministry of Agriculture, Livestock and Supply (MAPA). The antioxidant components analysis was used for traditional spectroscopic methods Folin-Ciocalteu (FC), Ferric Reducing Antioxidant Potential (FRAP), 2,2-diphenyl-1-picryl-hydrazyl (DPPH●); and electroanalytical calculated from the oxidation peaks voltammetric differential pulse (DPV) by Electrochemical Index (EI). While mid-infrared method (IR) and methods of multivariate statistical analysis were used for identification and classification of honey from different floral sources and/or regions. As for the deterioration analysis test (HMF and AD) 50% of the samples applied in these tests, for the control of physical and chemical quality, were at odds with the Normative Instruction N. 11/2000 of MAPA. All samples showed the presence of antioxidant compounds exposed by methods FC, FRAP, DPPH● and EI; and the method of FRAP was the one closest to the EI and the DPPH● was inversely proportional to EI, which may conclude that the electroanalytical by DPV is suitable for analysis of antioxidant compounds in honey. The infrared results coupled with chemometric tool, principal component analysis (PCA) and hierarchical cluster analysis (HCA), were not satisfactory for sample groups of the same floral origin and/or region, but suggested trend in results.O mel é uma fonte concentrada de alimento, é usada pelos animais e aproveitada como recurso natural pelos homens desde a antiguidade. Muitos são os estudos que trataram de caracterizar os méis e sua qualidade, mas o complexo processo de produção natural pelas abelhas faz que nenhuma pesquisa obtenha resultados plenamente satisfatórios. Neste trabalho foram estudadas 24 amostras provenientes de regiões produtoras de mel localizadas no entorno de Goiânia – GO e algumas amostras estrangeiras (Portugal, Estados Unidos e Nova Zelândia). Os testes físico-químicos empregados com indicativos de deterioração foram (de acordo com a Instrução Normativa nº 11/2000 do Ministério da Agricultura, Pecuária e Abastecimento - MAPA): o método do hidroximetilfurfural (HMF) e atividade diastásica (AD). Alem da avaliação da qualidade, foi analisada a capacidade antioxidante mediante: métodos espectrofotométricos tradicionais (Folin-Ciocalteau - FC, Ferric Reducing Antioxidant Potential - FRAP; e 2,2-difenil-1-picril-hidrazil - DPPH●); e métodos eletroanalíticos (Índice Eletroquímico - IE, calculado a partir dos picos de oxidação da voltametria de pulso diferencial - DPV). Os resultados obtidos por os diferentes métodos foram tratados usando técnicas de estatística descritiva e quimiometría como a análise de componentes principais (PCA) ou análise hierárquica (HCA), com a finalidade de classificar e identificar as méis a partir das fontes florais e/ou regiões. Os resultado das análises de deterioração (HMF e AD), 50% das amostras estavam em desacordo com a citada Norma. Respeito à atividade antioxidante, em todas as amostras, contrastou-se uma correlação entre os diferentes métodos de analises (FC, FRAP, DPPH● e IE). O método do FRAP foi o que mais se aproximou do IE e o DPPH● foi inversamente proporcional ao IE, o que pode concluir que a técnica eletroanalítica DPV é satisfatória para análise de compostos antioxidantes em mel.Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2015-12-04T08:42:21Z No. of bitstreams: 2 Dissertação - Stefani Garcia Rezende - 2015.pdf: 1241735 bytes, checksum: dafaf794ccbc058198ef0f2dd475ae74 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2015-12-04T08:43:59Z (GMT) No. of bitstreams: 2 Dissertação - Stefani Garcia Rezende - 2015.pdf: 1241735 bytes, checksum: dafaf794ccbc058198ef0f2dd475ae74 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Made available in DSpace on 2015-12-04T08:43:59Z (GMT). No. of bitstreams: 2 Dissertação - Stefani Garcia Rezende - 2015.pdf: 1241735 bytes, checksum: dafaf794ccbc058198ef0f2dd475ae74 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2015-03-30Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqapplication/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Ciências Farmacêuticas (FF)UFGBrasilFaculdade Farmácia - FF (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessAntioxidante em melVoltametria de pulso diferencialHidroximetilfurfuralAtividade diastásicaQuimiometriaAntioxidant in honeyDifferential pulse voltammetryHidroxymethtlfurfuralDiastatic activityChemometricsCIENCIAS BIOLOGICAS::FARMACOLOGIAMétodos eletroanalíticos e ensaios de atividade antioxidante no controle de qualidade de melinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis8249369881961524126006006006006010281161524209375700814650651154363-2555911436985713659reponame:Biblioteca Digital de Teses e Dissertações da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGORIGINALDissertação - Stefani Garcia Rezende - 2015.pdfDissertação - Stefani Garcia Rezende - 2015.pdfapplication/pdf1241735http://repositorio.bc.ufg.br/tede/bitstreams/63afbf33-047f-4ad1-ae87-b445abf66b03/downloaddafaf794ccbc058198ef0f2dd475ae74MD55LICENSElicense.txtlicense.txttext/plain; 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dc.title.por.fl_str_mv |
Métodos eletroanalíticos e ensaios de atividade antioxidante no controle de qualidade de mel |
title |
Métodos eletroanalíticos e ensaios de atividade antioxidante no controle de qualidade de mel |
spellingShingle |
Métodos eletroanalíticos e ensaios de atividade antioxidante no controle de qualidade de mel Rezende, Stefani Garcia Antioxidante em mel Voltametria de pulso diferencial Hidroximetilfurfural Atividade diastásica Quimiometria Antioxidant in honey Differential pulse voltammetry Hidroxymethtlfurfural Diastatic activity Chemometrics CIENCIAS BIOLOGICAS::FARMACOLOGIA |
title_short |
Métodos eletroanalíticos e ensaios de atividade antioxidante no controle de qualidade de mel |
title_full |
Métodos eletroanalíticos e ensaios de atividade antioxidante no controle de qualidade de mel |
title_fullStr |
Métodos eletroanalíticos e ensaios de atividade antioxidante no controle de qualidade de mel |
title_full_unstemmed |
Métodos eletroanalíticos e ensaios de atividade antioxidante no controle de qualidade de mel |
title_sort |
Métodos eletroanalíticos e ensaios de atividade antioxidante no controle de qualidade de mel |
author |
Rezende, Stefani Garcia |
author_facet |
Rezende, Stefani Garcia |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Gil, Eric de Souza |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3218622824233303 |
dc.contributor.advisor-co1.fl_str_mv |
Garcia, Telma Alves |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/6340261924930737 |
dc.contributor.referee1.fl_str_mv |
Gil, Eric de Souza |
dc.contributor.referee2.fl_str_mv |
Oliveira, Severino Carlos Bezerra |
dc.contributor.referee3.fl_str_mv |
Santos, Wallans Torres Pio dos |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/8713436663797736 |
dc.contributor.author.fl_str_mv |
Rezende, Stefani Garcia |
contributor_str_mv |
Gil, Eric de Souza Garcia, Telma Alves Gil, Eric de Souza Oliveira, Severino Carlos Bezerra Santos, Wallans Torres Pio dos |
dc.subject.por.fl_str_mv |
Antioxidante em mel Voltametria de pulso diferencial Hidroximetilfurfural Atividade diastásica Quimiometria |
topic |
Antioxidante em mel Voltametria de pulso diferencial Hidroximetilfurfural Atividade diastásica Quimiometria Antioxidant in honey Differential pulse voltammetry Hidroxymethtlfurfural Diastatic activity Chemometrics CIENCIAS BIOLOGICAS::FARMACOLOGIA |
dc.subject.eng.fl_str_mv |
Antioxidant in honey Differential pulse voltammetry Hidroxymethtlfurfural Diastatic activity Chemometrics |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::FARMACOLOGIA |
description |
The honey is a concentrated source of nutrients, it is used by animals and exploited as a natural resource for homes since antiquity. Many studies have tried to characterize the honey and its quality, but the complex natural process of production, involve that any research is not able to obtain fully satisfactory results. In this work 24 samples. Were studied 24 samples from honey producing regions located around Goiania - GO and some foreign samples (Portugual, USA and New Zelândias), those donated by the Beekeeping Association of the State of Goiás and these purchased in specialty stores in their respective countries . The physical and chemical tests as indicative of deterioration, hydroxymethylfurfural (HMF) and diastase activity (AD), followed parameters proposed by Normative Instruction N. 11/2000 of the Ministry of Agriculture, Livestock and Supply (MAPA). The antioxidant components analysis was used for traditional spectroscopic methods Folin-Ciocalteu (FC), Ferric Reducing Antioxidant Potential (FRAP), 2,2-diphenyl-1-picryl-hydrazyl (DPPH●); and electroanalytical calculated from the oxidation peaks voltammetric differential pulse (DPV) by Electrochemical Index (EI). While mid-infrared method (IR) and methods of multivariate statistical analysis were used for identification and classification of honey from different floral sources and/or regions. As for the deterioration analysis test (HMF and AD) 50% of the samples applied in these tests, for the control of physical and chemical quality, were at odds with the Normative Instruction N. 11/2000 of MAPA. All samples showed the presence of antioxidant compounds exposed by methods FC, FRAP, DPPH● and EI; and the method of FRAP was the one closest to the EI and the DPPH● was inversely proportional to EI, which may conclude that the electroanalytical by DPV is suitable for analysis of antioxidant compounds in honey. The infrared results coupled with chemometric tool, principal component analysis (PCA) and hierarchical cluster analysis (HCA), were not satisfactory for sample groups of the same floral origin and/or region, but suggested trend in results. |
publishDate |
2015 |
dc.date.accessioned.fl_str_mv |
2015-12-04T08:43:59Z |
dc.date.issued.fl_str_mv |
2015-03-30 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
REZENDE, S. G. Métodos eletroanalíticos e ensaios de atividade antioxidante no controle de qualidade de mel. 2015. 53 f. Dissertação (Mestrado em Ciências Farmacêuticas) - Universidade Federal de Goiás, Goiânia, 2015. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/4996 |
identifier_str_mv |
REZENDE, S. G. Métodos eletroanalíticos e ensaios de atividade antioxidante no controle de qualidade de mel. 2015. 53 f. Dissertação (Mestrado em Ciências Farmacêuticas) - Universidade Federal de Goiás, Goiânia, 2015. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/4996 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
824936988196152412 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 |
dc.relation.department.fl_str_mv |
6010281161524209375 |
dc.relation.cnpq.fl_str_mv |
700814650651154363 |
dc.relation.sponsorship.fl_str_mv |
-2555911436985713659 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Ciências Farmacêuticas (FF) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Faculdade Farmácia - FF (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
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institution |
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collection |
Biblioteca Digital de Teses e Dissertações da UFG |
bitstream.url.fl_str_mv |
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bitstream.checksum.fl_str_mv |
dafaf794ccbc058198ef0f2dd475ae74 bd3efa91386c1718a7f26a329fdcb468 4afdbb8c545fd630ea7db775da747b2f ae2fe251842ade1134c5d9bb99b6eefe 9da0b6dfac957114c6a7714714b86306 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UFG - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
tesesdissertacoes.bc@ufg.br ||tesesdissertacoes.bc@ufg.br |
_version_ |
1797047707728084992 |