Efeitos de Morus nigra L. (amoreira negra) sobre a resposta inflamatória e modulação de neutrófilos: estudos in vivo e in vitro

• Ano de defesa: 2016
• Autor(a) principal: CASTRO, Ahirlan Silva de
• Orientador(a): BORGES, Antônio Carlos Romão
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Maranhão
• Programa de Pós-Graduação: PROGRAMA DE PÓS-GRADUAÇÃO EM BIOTECNOLOGIA - RENORBIO/CCBS
• Departamento: DEPARTAMENTO DE BIOLOGIA/CCBS
• País: Brasil
• Palavras-chave em Português: Morus nigra L.; Inflamação aguda; Neutrófilos; Anti-inflamatório; Acute inflammation
• Palavras-chave em Inglês: Rats; Neutrophils; Anti-inflammation
• Área do conhecimento CNPq: Farmacognosia; Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPQ)
• Link de acesso: http://tedebc.ufma.br:8080/jspui/handle/tede/1501

Inflammation comprises a biological mechanism of the body's defense in response to injury by exogenous or endogenous agents and aims to restore homeostasis. Morus nigra L., commonly known as black mulberry tree is traditionally used to treat inflammatory diseases. Thus, the aim of this study was to evaluate the pharmacological potential of the hydroalcoholic extract and ethyl acetate fraction of Morus nigra leaves and the likely mechanisms of action in models of inflammation in vivo and in vitro involving neutrophils and mediators of the inflammatory process. Therefore, the dried leaves were sprayed and soaked in 70% ethanol to obtain the hydroalcoholic extract (EHA). EHA was subjected to partitioning with hexane (FH), chloroform (FC) and ethyl acetate (FAE). Quantification of phenolic and total flavonoid compounds showed higher levels of these secondary metabolites in the EHA and FAE. Analysis by HPLC-UV showed the presence of chlorogenic acid, rutin, quercetin and kaempferol in EHA and FAE of Morus nigra. In the acute toxicology studies, treatment of male and female rats with EHA (2000 mg / kg, o.v.) in a single dose produced no death, nor change in consciousness, motor and autonomic and central nervous system; body weight and organs; consumption of water and food; haematological and biochemical parameters; when compared to the control group. EHA and FAE of Morus nigra presented antiedematogenic and anti-inflammatory activity by reducing the paw edema induced by carrageenan. EHA and FAE decreased migration of leukocytes and neutrophils for subcutaneous air pouch inflammatory stimulation by carrageenan, inhibiting TNF-α production and MPO. EHA and FAE also promoted decreased migration of leukocytes and neutrophils into the peritoneal cavity of rats stimulated with LPS, decreasing the production of NO, TNF- α, IL-1β and IL-8. Further, the EHA and FAE of Morus nigra decreased neutrophils chemotaxis in Boyden chamber assay modified front of chemotactic agents fMLP and IL-8. Together the data presented demonstrate that the EHA and FAE of Morus nigra had significant anti-inflammatory activity and can thus be used to treat inflammatory processes.