Efeito do resveratrol complexado à hidroxipropil-β-ciclodextrina sobre parâmetros do estresse oxidativo em ratos normais e hiperglicêmicos
Ano de defesa: | 2014 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Franciscana
|
Programa de Pós-Graduação: |
Mestrado Acadêmico em Nanociências
|
Departamento: |
Biociências e Nanomateriais
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/229 http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/309 |
Resumo: | The use of nanoparticles as carriers of drugs has been widely studied. These include cyclodextrins (CDs), cyclic oligosaccharides that allow the formation of inclusion complexes with lipohilic substances such as resveratrol (RSV). RSV is a polyphenol with antioxidant characteristics; when consumed, a great part is excreted, while the remainder is quickly metabolized in the body. The anti-inflammatory, antioxidant and preventive of metabolic diseased properties such as diabetes, stimulate the complexation of RSV to the hydroxypropyl-beta-cyclodextrin (HP-β-CD), thus enabling the increase of the bioavailability of an orally RSV. Hyperglycemia, a marker by which diabetes is diagnosed, promotes increased production of species reactive to oxygen by various routes that lead to cell damage and potentially to cell death. The oxidative stress parameters can be determined through certain techniques. The reduction in the amount or in the activity of endogenous antioxidant defenses is and indicative of oxidant injury and may, therefore, is considered as an excellent marker of oxidative stress. In this context, nanotechnology can contribute to the development of new therapeutic approaches for various diseases, including diabetes. The objective of this study is to investigate the in vivo effects of free and complex resveratrol to HP-β-CD on parameters of oxidative stress, based on the measurements of GSH, TBARS, total content of carbonyls, activity of SOD, CAT and GPx enzymes and determination of total protein in the cerebral cortex, liver, kidney and heart of normal and hyperglycemic rats. For this study, 70 male Wistar rats were used, form the vivarium of the Federal University of Santa Maria, randomly divided into five diabetic groups induced by streptozotocin (STZ), and five non-diabetic groups with seven animals per group. The hyperglycemic and normoglycemic groups daily received, through intragastric gavage, the following substances: water (control groups), free RSV in concentration of 1mg/kg (RSV groups), concentration of HP- β-CD equivalent to that present in complex (HP- β-CD groups), RSV complexed to the cyclodextrin at a concentration of 1 mg/kg body weight (complex groups) and a solution of 15% ethanol (ethanol group). After two months of treatment and 12 hours fasting, the animals were killed by decapitation. According to the data obtained, it was found that treatment with HP- β-CD caused a decrease of endogenous antioxidant molecules. When complexed to RSV, this decrease was lower; indicating a protective effect by RSV to the damage caused by the medium, in this case HP- β-CD. In the measurements of TBARS it was observed that there was a decrease in the RSV group and HP- β-CD, indicating a protective behavior to lipid peroxidation. |
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Fernandes, Liana da SilvaCPF:00140712070http://lattes.cnpq.br/0573570242395130Rech, Virginia CieloCPF:58768211015CPF:56768443020http://lattes.cnpq.br/9719396661436116 56768443020Pinto, Claudia Grigolo2018-06-27T18:56:14Z2014-03-31PINTO, Claudia Grigolo. Efeito do resveratrol complexado à hidroxipropil-β-ciclodextrina sobre parâmetros do estresse oxidativo em ratos normais e hiperglicêmicos. 2014. 71 f. Dissertação (Mestrado em Biociências e Nanomateriais) - Universidade Franciscana, Santa Maria, 2014.http://tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/229http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/309The use of nanoparticles as carriers of drugs has been widely studied. These include cyclodextrins (CDs), cyclic oligosaccharides that allow the formation of inclusion complexes with lipohilic substances such as resveratrol (RSV). RSV is a polyphenol with antioxidant characteristics; when consumed, a great part is excreted, while the remainder is quickly metabolized in the body. The anti-inflammatory, antioxidant and preventive of metabolic diseased properties such as diabetes, stimulate the complexation of RSV to the hydroxypropyl-beta-cyclodextrin (HP-β-CD), thus enabling the increase of the bioavailability of an orally RSV. Hyperglycemia, a marker by which diabetes is diagnosed, promotes increased production of species reactive to oxygen by various routes that lead to cell damage and potentially to cell death. The oxidative stress parameters can be determined through certain techniques. The reduction in the amount or in the activity of endogenous antioxidant defenses is and indicative of oxidant injury and may, therefore, is considered as an excellent marker of oxidative stress. In this context, nanotechnology can contribute to the development of new therapeutic approaches for various diseases, including diabetes. The objective of this study is to investigate the in vivo effects of free and complex resveratrol to HP-β-CD on parameters of oxidative stress, based on the measurements of GSH, TBARS, total content of carbonyls, activity of SOD, CAT and GPx enzymes and determination of total protein in the cerebral cortex, liver, kidney and heart of normal and hyperglycemic rats. For this study, 70 male Wistar rats were used, form the vivarium of the Federal University of Santa Maria, randomly divided into five diabetic groups induced by streptozotocin (STZ), and five non-diabetic groups with seven animals per group. The hyperglycemic and normoglycemic groups daily received, through intragastric gavage, the following substances: water (control groups), free RSV in concentration of 1mg/kg (RSV groups), concentration of HP- β-CD equivalent to that present in complex (HP- β-CD groups), RSV complexed to the cyclodextrin at a concentration of 1 mg/kg body weight (complex groups) and a solution of 15% ethanol (ethanol group). After two months of treatment and 12 hours fasting, the animals were killed by decapitation. According to the data obtained, it was found that treatment with HP- β-CD caused a decrease of endogenous antioxidant molecules. When complexed to RSV, this decrease was lower; indicating a protective effect by RSV to the damage caused by the medium, in this case HP- β-CD. In the measurements of TBARS it was observed that there was a decrease in the RSV group and HP- β-CD, indicating a protective behavior to lipid peroxidation.O emprego de nanopartículas como carreadores de fármacos vem sendo vastamente estudado. Entre elas, estão as ciclodextrinas (CDs), que são oligossacarídeos cíclicos que permitem a formação de complexos de inclusão com substâncias lipofílicas, como o resveratrol (RSV). O RSV é um polifenol com características antioxidantes; quando consumido, há uma grande parte excretada, enquanto o restante é rapidamente metabolizado no organismo. As propriedades antioxidantes, anti-inflamatórias e preventivas de doenças metabólicas, como o diabetes, estimulam a complexação do RSV à hidróxi-propil-beta-ciclodextrina (HP--CD), possibilitando, dessa forma, o aumento da biodisponibilidade do RSV por via oral. A hiperglicemia, marcador por meio do qual se diagnostica o diabetes, promove aumento na produção de espécies reativas de oxigênio por diversas rotas, que levam a lesões celulares e potencialmente à morte celular. Os parâmetros de estresse oxidativo podem ser determinados através de diversas técnicas. A redução da quantidade ou da atividade das defesas antioxidantes endógenas é indicativa de lesão oxidante, podendo, portanto, ser considerada como excelente marcador de estresse oxidativo. Nesse contexto, a nanotecnologia pode contribuir para o desenvolvimento de novas abordagens terapêuticas para diversas doenças, entre elas o diabetes. O objetivo deste trabalho é investigar o efeito in vivo do resveratrol livre e complexado à HP-β-CD sobre parâmetros do estresse oxidativo, com base nas medidas de Glutationa reduzida (GSH), espécies reativas ao ácido tiobarbitúrico (TBARS), conteúdo total de carbonilas, atividade das enzimas superóxido dismutase (SOD), catalase (CAT), glutationa peroxidase (GPx) e dosagem de proteínas totais, no córtex cerebral, no fígado, nos rins e no coração de ratos normais e hiperglicêmicos. Para este trabalho, foram utilizados 70 ratos Wistar machos, provenientes do biotério da Universidade Federal de Santa Maria, separados aleatoriamente em cinco grupos diabéticos, induzidos por estreptozotocina (STZ) e cinco grupos não diabéticos, com sete animais por grupo. Os grupos normoglicêmicos e hiperglicêmicos receberam diariamente, por gavagem intragástrica, as seguintes substâncias: grupos controles receberam água, os grupos RSV receberam 1mg/kg de massa corporal, os grupos HP-β-CD receberam concentração de HP-β-CD equivalente àquela presente no complexo, os grupos complexo (RCD) receberam 1mg/kg e os grupos etanol (E) receberam solução de etanol a 15%. Os animais foram mortos, após dois meses de tratamento e com 12 horas em jejum, por decapitação, sem anestesia. De acordo com os dados obtidos, concluiu-se que o tratamento com HP-β-CD provocou uma queda das moléculas antioxidantes endógenas. Quando complexada ao RSV, tal queda foi menor, indicando uma proteção exercida pelo RSV ao dano causado pelo veículo, neste caso a HP-β-CD. Nas medidas de TBARS, observou-se que ocorreu uma queda nos grupos RSV e HP-β-CD o que indica um comportamento de proteção à lipoperoxidação.Made available in DSpace on 2018-06-27T18:56:14Z (GMT). No. of bitstreams: 3 Claudia Grigolo Pinto.pdf: 1176064 bytes, checksum: 59a91895834e923c2658353b7e595164 (MD5) Claudia Grigolo Pinto.pdf.txt: 107937 bytes, checksum: 1b0f8213d3d5c275ed204303dd4db6c4 (MD5) Claudia Grigolo Pinto.pdf.jpg: 3006 bytes, checksum: 39d1ff3d9bf0d2f9de94bcb4adf42377 (MD5) Previous issue date: 2014-03-31Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfhttp://tede.universidadefranciscana.edu.br:8080/retrieve/464/Claudia%20Grigolo%20Pinto.pdf.jpgporUniversidade FranciscanaMestrado Acadêmico em NanociênciasUFNBRBiociências e NanomateriaisNanotecnologiaciclodextrinasresveratrolestresse oxidativoNanotechnologyciclodextrinsresveratroloxidative stressCNPQ::ENGENHARIASEfeito do resveratrol complexado à hidroxipropil-β-ciclodextrina sobre parâmetros do estresse oxidativo em ratos normais e hiperglicêmicosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional Universidade Franciscanainstname:Universidade Franciscana (UFN)instacron:UFNORIGINALClaudia Grigolo Pinto.pdfapplication/pdf1176064http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/309/1/Claudia+Grigolo+Pinto.pdf59a91895834e923c2658353b7e595164MD51TEXTClaudia Grigolo Pinto.pdf.txttext/plain107937http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/309/2/Claudia+Grigolo+Pinto.pdf.txt1b0f8213d3d5c275ed204303dd4db6c4MD52THUMBNAILClaudia Grigolo Pinto.pdf.jpgimage/jpeg3006http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/309/3/Claudia+Grigolo+Pinto.pdf.jpg39d1ff3d9bf0d2f9de94bcb4adf42377MD53UFN-BDTD/3092018-06-27 15:56:14.889oai:tede.universidadefranciscana.edu.br:UFN-BDTD/309Repositório de Publicaçõeshttp://www.tede.universidadefranciscana.edu.br:8080/http://www.tede.universidadefranciscana.edu.br:8080/oai/requestopendoar:2018-06-27T18:56:14Repositório Institucional Universidade Franciscana - Universidade Franciscana (UFN)false |
dc.title.por.fl_str_mv |
Efeito do resveratrol complexado à hidroxipropil-β-ciclodextrina sobre parâmetros do estresse oxidativo em ratos normais e hiperglicêmicos |
title |
Efeito do resveratrol complexado à hidroxipropil-β-ciclodextrina sobre parâmetros do estresse oxidativo em ratos normais e hiperglicêmicos |
spellingShingle |
Efeito do resveratrol complexado à hidroxipropil-β-ciclodextrina sobre parâmetros do estresse oxidativo em ratos normais e hiperglicêmicos Pinto, Claudia Grigolo Nanotecnologia ciclodextrinas resveratrol estresse oxidativo Nanotechnology ciclodextrins resveratrol oxidative stress CNPQ::ENGENHARIAS |
title_short |
Efeito do resveratrol complexado à hidroxipropil-β-ciclodextrina sobre parâmetros do estresse oxidativo em ratos normais e hiperglicêmicos |
title_full |
Efeito do resveratrol complexado à hidroxipropil-β-ciclodextrina sobre parâmetros do estresse oxidativo em ratos normais e hiperglicêmicos |
title_fullStr |
Efeito do resveratrol complexado à hidroxipropil-β-ciclodextrina sobre parâmetros do estresse oxidativo em ratos normais e hiperglicêmicos |
title_full_unstemmed |
Efeito do resveratrol complexado à hidroxipropil-β-ciclodextrina sobre parâmetros do estresse oxidativo em ratos normais e hiperglicêmicos |
title_sort |
Efeito do resveratrol complexado à hidroxipropil-β-ciclodextrina sobre parâmetros do estresse oxidativo em ratos normais e hiperglicêmicos |
author |
Pinto, Claudia Grigolo |
author_facet |
Pinto, Claudia Grigolo |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Fernandes, Liana da Silva |
dc.contributor.advisor1ID.fl_str_mv |
CPF:00140712070 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/0573570242395130 |
dc.contributor.advisor-co1.fl_str_mv |
Rech, Virginia Cielo |
dc.contributor.advisor-co1ID.fl_str_mv |
CPF:58768211015 |
dc.contributor.authorID.fl_str_mv |
CPF:56768443020 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/9719396661436116 56768443020 |
dc.contributor.author.fl_str_mv |
Pinto, Claudia Grigolo |
contributor_str_mv |
Fernandes, Liana da Silva Rech, Virginia Cielo |
dc.subject.por.fl_str_mv |
Nanotecnologia ciclodextrinas resveratrol estresse oxidativo |
topic |
Nanotecnologia ciclodextrinas resveratrol estresse oxidativo Nanotechnology ciclodextrins resveratrol oxidative stress CNPQ::ENGENHARIAS |
dc.subject.eng.fl_str_mv |
Nanotechnology ciclodextrins resveratrol oxidative stress |
dc.subject.cnpq.fl_str_mv |
CNPQ::ENGENHARIAS |
description |
The use of nanoparticles as carriers of drugs has been widely studied. These include cyclodextrins (CDs), cyclic oligosaccharides that allow the formation of inclusion complexes with lipohilic substances such as resveratrol (RSV). RSV is a polyphenol with antioxidant characteristics; when consumed, a great part is excreted, while the remainder is quickly metabolized in the body. The anti-inflammatory, antioxidant and preventive of metabolic diseased properties such as diabetes, stimulate the complexation of RSV to the hydroxypropyl-beta-cyclodextrin (HP-β-CD), thus enabling the increase of the bioavailability of an orally RSV. Hyperglycemia, a marker by which diabetes is diagnosed, promotes increased production of species reactive to oxygen by various routes that lead to cell damage and potentially to cell death. The oxidative stress parameters can be determined through certain techniques. The reduction in the amount or in the activity of endogenous antioxidant defenses is and indicative of oxidant injury and may, therefore, is considered as an excellent marker of oxidative stress. In this context, nanotechnology can contribute to the development of new therapeutic approaches for various diseases, including diabetes. The objective of this study is to investigate the in vivo effects of free and complex resveratrol to HP-β-CD on parameters of oxidative stress, based on the measurements of GSH, TBARS, total content of carbonyls, activity of SOD, CAT and GPx enzymes and determination of total protein in the cerebral cortex, liver, kidney and heart of normal and hyperglycemic rats. For this study, 70 male Wistar rats were used, form the vivarium of the Federal University of Santa Maria, randomly divided into five diabetic groups induced by streptozotocin (STZ), and five non-diabetic groups with seven animals per group. The hyperglycemic and normoglycemic groups daily received, through intragastric gavage, the following substances: water (control groups), free RSV in concentration of 1mg/kg (RSV groups), concentration of HP- β-CD equivalent to that present in complex (HP- β-CD groups), RSV complexed to the cyclodextrin at a concentration of 1 mg/kg body weight (complex groups) and a solution of 15% ethanol (ethanol group). After two months of treatment and 12 hours fasting, the animals were killed by decapitation. According to the data obtained, it was found that treatment with HP- β-CD caused a decrease of endogenous antioxidant molecules. When complexed to RSV, this decrease was lower; indicating a protective effect by RSV to the damage caused by the medium, in this case HP- β-CD. In the measurements of TBARS it was observed that there was a decrease in the RSV group and HP- β-CD, indicating a protective behavior to lipid peroxidation. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-03-31 |
dc.date.accessioned.fl_str_mv |
2018-06-27T18:56:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
PINTO, Claudia Grigolo. Efeito do resveratrol complexado à hidroxipropil-β-ciclodextrina sobre parâmetros do estresse oxidativo em ratos normais e hiperglicêmicos. 2014. 71 f. Dissertação (Mestrado em Biociências e Nanomateriais) - Universidade Franciscana, Santa Maria, 2014. |
dc.identifier.uri.fl_str_mv |
http://tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/229 http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/309 |
identifier_str_mv |
PINTO, Claudia Grigolo. Efeito do resveratrol complexado à hidroxipropil-β-ciclodextrina sobre parâmetros do estresse oxidativo em ratos normais e hiperglicêmicos. 2014. 71 f. Dissertação (Mestrado em Biociências e Nanomateriais) - Universidade Franciscana, Santa Maria, 2014. |
url |
http://tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/229 http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/309 |
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Universidade Franciscana |
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Mestrado Acadêmico em Nanociências |
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UFN |
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BR |
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Biociências e Nanomateriais |
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Universidade Franciscana |
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