PRODUÇÃO, CARACTERIZAÇÃO E ESTUDOS IN VITRO E IN VIVO DE VESÍCULAS LIPOSSOMAIS CONTENDO α-ARBUTIN, VISANDO APLICAÇÃO NO TRATAMENTO DE MELASMA

• Ano de defesa: 2021
• Autor(a) principal: Poleze, Thatyana Cassol
• Orientador(a): Mortari, Sérgio Roberto
• Banca de defesa: Fontanive, Tiago Oselane, Silva, Ivana Zanella da
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso embargado
• Idioma: por
• Instituição de defesa: Universidade Franciscana
• Programa de Pós-Graduação: Programa de Pós-Graduação em Nanociências
• Departamento: Biociências e Nanomateriais
• País: Brasil
• Palavras-chave em Português: Ativo; nanotecnologia; pele; hidratação; permeação.
• Palavras-chave em Inglês: Active; nanotechnology; skin; hydration; permeation.
• Área do conhecimento CNPq: Nanociências
• Link de acesso: http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/999

Melasma is easy to diagnose, but difficult to cure. It affects women of childbearing age most, affecting only 10% of men. There are already several treatments on the market, but none that are 100% efficient since it has a high recurrence rate. The appearance of dark spots on the skin (melasma), in a way, interferes with the quality of life, since this condition affects the person's self-esteem. In the search for the efficiency of a treatment with less side effects, the use of nanotechnology has presented formulations that meet these needs. Thus, producing new formulations, on a nanoscale, can be an alternative to increase efficiency in treatment, since it improves hydration, skin permeation and can also reduce side effects, also increasing the effectiveness of the active ingredient. In this sense, this work aimed to produce, characterize and carry out studies, in vitro and in vivo, of a liposomal source containing α-arbutin, for application in the treatment of melasma. The formulations were supplied by the ethanol injection method and remained stable for up to 60 days when stored at room temperature and under refrigeration. The liposomal vesicles had an average diameter between 140 nm to 175 nm, polydispersion index below 0.20, zeta potential between -26.0 to -10.0 mV, and pH between 5.0-5.5, simulating the pH values of human skin. For skin biometrics studies, liposomal information, containing α-arbutin was incorporated in a gel-cream, showing stability is up to 60 days when stored at room temperature or under refrigeration. In vitro studies, carried out on keratinocytes, have shown that they do not alter the viability of normal skin cells, thus showing good safety for topical application. The results of skin evaluation, with women between 18 and 50 years old, demonstrated the voluntary safety for the use of the liposomal diet containing α-arbutin, incorporated in gel-cream, since none of the women arouses an adverse reaction to the nanotechnological product, in the period of proposed progress.