Identificação molecular e perfil sorológico de Leptospira spp. isolada de gambás-de-orelha-branca (Didelphis albiventris) no sul do Brasil
Ano de defesa: | 2009 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Pelotas
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Veterinária
|
Departamento: |
Veterinária
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufpel.edu.br/handle/ri/2539 |
Resumo: | Leptospirosis is a zoonotic disease that occurs all over the world, particularly in developing countries, caused by bacteria of the genus Leptospira. Several marsupial species are considered susceptible to infection caused by a wide variety of Leptospira serovars, acting as reservoirs. In this work were collected serum and urine samples of 33 White-eared opossum, trapped within distinct locations of Capão do Leão and Pelotas cities, in the South of Brazil, aiming to detect antibodies and to isolate leptospires. Serum samples were screened against a panel of 58 Leptospira spp and Leptonema illini serovars using the microscopic agglutination test (MAT). To attempt isolation, urine samples from all animals were inoculated in culture medium.EMJH enriched with 10% of supplement Difco® One pathogenic serovar was isolated after two months of inoculation, and is first isolate the country for this animal species and was characterized by PCR using primers for 16S and Lipl32 genes. The technique of partial sequencing of the rpoB gene was used to identify the genomic specie. The isolated strain belongs to the specie L. borgpetersenii, which along with L .interrogans are the main cause disease in humans. The isolate, was tested on MAT using dog, cattle and human sera. Cut off titer of 50 was used for domestic animal sera and 25 for human and opossum sera. Both human and animal sera presented agglutination on MAT to isolated strain corresponding to 3.3% (2/60), 28.33% (17/60) and 1.67% (1/60) in human, dog and cattle sera, respectively. The opossum sera presented 36.36% (12/33) of reaction. These findings suggest a probable white-eared opossum role in the maintenance of pathogenic Leptospira on the environment since low titer sera and urine elimination were observed. These animals could be important reservoirs of Leptospira serovars for human and domestic animals. |
id |
UFPL_72369c8107738ae43fe29ec5ae1cf6e0 |
---|---|
oai_identifier_str |
oai:guaiaca.ufpel.edu.br:123456789/2539 |
network_acronym_str |
UFPL |
network_name_str |
Repositório Institucional da UFPel - Guaiaca |
repository_id_str |
|
spelling |
2014-08-20T14:37:58Z2009-10-272014-08-20T14:37:58Z2009-07-20JORGE, Sérgio. Molecular identification and serological profile of Leptospira spp. isolated of white-eared-opossum (Didelphis albiventris) in South of Brazil. 2009. 57 f. Dissertação (Mestrado em Veterinária) - Universidade Federal de Pelotas, Pelotas, 2009.http://repositorio.ufpel.edu.br/handle/ri/2539Leptospirosis is a zoonotic disease that occurs all over the world, particularly in developing countries, caused by bacteria of the genus Leptospira. Several marsupial species are considered susceptible to infection caused by a wide variety of Leptospira serovars, acting as reservoirs. In this work were collected serum and urine samples of 33 White-eared opossum, trapped within distinct locations of Capão do Leão and Pelotas cities, in the South of Brazil, aiming to detect antibodies and to isolate leptospires. Serum samples were screened against a panel of 58 Leptospira spp and Leptonema illini serovars using the microscopic agglutination test (MAT). To attempt isolation, urine samples from all animals were inoculated in culture medium.EMJH enriched with 10% of supplement Difco® One pathogenic serovar was isolated after two months of inoculation, and is first isolate the country for this animal species and was characterized by PCR using primers for 16S and Lipl32 genes. The technique of partial sequencing of the rpoB gene was used to identify the genomic specie. The isolated strain belongs to the specie L. borgpetersenii, which along with L .interrogans are the main cause disease in humans. The isolate, was tested on MAT using dog, cattle and human sera. Cut off titer of 50 was used for domestic animal sera and 25 for human and opossum sera. Both human and animal sera presented agglutination on MAT to isolated strain corresponding to 3.3% (2/60), 28.33% (17/60) and 1.67% (1/60) in human, dog and cattle sera, respectively. The opossum sera presented 36.36% (12/33) of reaction. These findings suggest a probable white-eared opossum role in the maintenance of pathogenic Leptospira on the environment since low titer sera and urine elimination were observed. These animals could be important reservoirs of Leptospira serovars for human and domestic animals.A Leptospirose é uma antropozoonose de ocorrência mundial, particularmente nos países em desenvolvimento, causada por bactérias do gênero Leptospira. Várias espécies de marsupiais e didelfídeos são consideradas suscetíveis a infecção causada por uma grande variedade de sorovares patogênicos de Leptospira spp. tendo sido considerados possíveis hospedeiros deste agente. Neste trabalho foram coletadas amostras de soro e urina de 33 gambás-de-orelha-branca (Didelphis albiventris), capturados em diferentes regiões dos municípios do Capão do Leão e Pelotas, no sul do Brasil, com o objetivo de detectar anticorpos e isolar leptospiras. As amostras de soro foram testadas por aglutinação microscópica (MAT), utilizando 58 sorovares de Leptospira spp. e 1 de Leptonema. Para o isolamento, amostras de urina foram inoculadas em meio de cultura EMJH enriquecido com 10% de suplemento Difco®. Um sorovar patogênico foi obtido da urina, sendo o primeiro isolado do país para esta espécie, e foi caracterizado quanto ao gênero e patogenicidade por PCR utilizando primers para os genes 16S rDNA e Lipl32. A técnica de seqüenciamento parcial do gene rpoB foi utilizada para identificar a espécie genômica. A cepa isolada pertence à espécie L. borgpertersenii,, que juntamente com L. interrogans são as principais causadoras de doença em humanos. Anticorpos anti isolado de gambá foram avaliados em amostras sorológicas de 60 cães, 60 bovinos, 60 humanos e dos 33 gambás capturados através da MAT. Os soros humanos apresentaram reação de 3,3% (2/60), 28,33% (17/60) em soros caninos, 1,67% (1/60) em soros bovinos e 36,36% (12/33) em soros dos gambás capturados no estudo. Esses dados sugerem o envolvimento do gambá-de-orelha-branca na manutenção de leptospiras no ambiente, uma vez que estando infectados por sorovares patogênicos podem eliminar o agente pela urina e infectar direta ou indiretamente animais domésticos e conseqüentemente o homem.application/pdfporUniversidade Federal de PelotasPrograma de Pós-Graduação em VeterináriaUFPelBRVeterináriaZoonosesLeptospiroseAnimais silvestresIsolamentoDiagnóstico sorológicoGambás-de-orelha brancaDidelphis albiventrisLeptospirosisWhite-eared opossumDidelphis albiventrisZoonosisPCRrpoB sequencingCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAIdentificação molecular e perfil sorológico de Leptospira spp. isolada de gambás-de-orelha-branca (Didelphis albiventris) no sul do BrasilMolecular identification and serological profile of Leptospira spp. isolated of white-eared-opossum (Didelphis albiventris) in South of Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttp://lattes.cnpq.br/4952257308264821http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780308T3Fernandes, Cláudia Pinho Hartlebenlattes.cnpq.br/8784620034013411Brod, Claudiomar SoaresJorge, Sérgioinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPel - Guaiacainstname:Universidade Federal de Pelotas (UFPEL)instacron:UFPELORIGINALdissertacao_sergio_jorge.pdfapplication/pdf980348http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2539/1/dissertacao_sergio_jorge.pdf9e56cf9be902454868ace419c988ca33MD51open accessTEXTdissertacao_sergio_jorge.pdf.txtdissertacao_sergio_jorge.pdf.txtExtracted Texttext/plain77787http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2539/2/dissertacao_sergio_jorge.pdf.txt4e9cc8a7d5c5d3a4297ed9c116076933MD52open accessTHUMBNAILdissertacao_sergio_jorge.pdf.jpgdissertacao_sergio_jorge.pdf.jpgGenerated Thumbnailimage/jpeg1768http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2539/3/dissertacao_sergio_jorge.pdf.jpg011437cd7f5bc918938491dca2e665dfMD53open access123456789/25392019-09-25 11:30:37.006open accessoai:guaiaca.ufpel.edu.br:123456789/2539Repositório InstitucionalPUBhttp://repositorio.ufpel.edu.br/oai/requestrippel@ufpel.edu.br || repositorio@ufpel.edu.br || aline.batista@ufpel.edu.bropendoar:2019-09-25T14:30:37Repositório Institucional da UFPel - Guaiaca - Universidade Federal de Pelotas (UFPEL)false |
dc.title.por.fl_str_mv |
Identificação molecular e perfil sorológico de Leptospira spp. isolada de gambás-de-orelha-branca (Didelphis albiventris) no sul do Brasil |
dc.title.alternative.eng.fl_str_mv |
Molecular identification and serological profile of Leptospira spp. isolated of white-eared-opossum (Didelphis albiventris) in South of Brazil |
title |
Identificação molecular e perfil sorológico de Leptospira spp. isolada de gambás-de-orelha-branca (Didelphis albiventris) no sul do Brasil |
spellingShingle |
Identificação molecular e perfil sorológico de Leptospira spp. isolada de gambás-de-orelha-branca (Didelphis albiventris) no sul do Brasil Jorge, Sérgio Zoonoses Leptospirose Animais silvestres Isolamento Diagnóstico sorológico Gambás-de-orelha branca Didelphis albiventris Leptospirosis White-eared opossum Didelphis albiventris Zoonosis PCR rpoB sequencing CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Identificação molecular e perfil sorológico de Leptospira spp. isolada de gambás-de-orelha-branca (Didelphis albiventris) no sul do Brasil |
title_full |
Identificação molecular e perfil sorológico de Leptospira spp. isolada de gambás-de-orelha-branca (Didelphis albiventris) no sul do Brasil |
title_fullStr |
Identificação molecular e perfil sorológico de Leptospira spp. isolada de gambás-de-orelha-branca (Didelphis albiventris) no sul do Brasil |
title_full_unstemmed |
Identificação molecular e perfil sorológico de Leptospira spp. isolada de gambás-de-orelha-branca (Didelphis albiventris) no sul do Brasil |
title_sort |
Identificação molecular e perfil sorológico de Leptospira spp. isolada de gambás-de-orelha-branca (Didelphis albiventris) no sul do Brasil |
author |
Jorge, Sérgio |
author_facet |
Jorge, Sérgio |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/4952257308264821 |
dc.contributor.advisorLattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780308T3 |
dc.contributor.advisor-co1.fl_str_mv |
Fernandes, Cláudia Pinho Hartleben |
dc.contributor.advisor-co1Lattes.fl_str_mv |
lattes.cnpq.br/8784620034013411 |
dc.contributor.advisor1.fl_str_mv |
Brod, Claudiomar Soares |
dc.contributor.author.fl_str_mv |
Jorge, Sérgio |
contributor_str_mv |
Fernandes, Cláudia Pinho Hartleben Brod, Claudiomar Soares |
dc.subject.por.fl_str_mv |
Zoonoses Leptospirose Animais silvestres Isolamento Diagnóstico sorológico Gambás-de-orelha branca Didelphis albiventris |
topic |
Zoonoses Leptospirose Animais silvestres Isolamento Diagnóstico sorológico Gambás-de-orelha branca Didelphis albiventris Leptospirosis White-eared opossum Didelphis albiventris Zoonosis PCR rpoB sequencing CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
Leptospirosis White-eared opossum Didelphis albiventris Zoonosis PCR rpoB sequencing |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
Leptospirosis is a zoonotic disease that occurs all over the world, particularly in developing countries, caused by bacteria of the genus Leptospira. Several marsupial species are considered susceptible to infection caused by a wide variety of Leptospira serovars, acting as reservoirs. In this work were collected serum and urine samples of 33 White-eared opossum, trapped within distinct locations of Capão do Leão and Pelotas cities, in the South of Brazil, aiming to detect antibodies and to isolate leptospires. Serum samples were screened against a panel of 58 Leptospira spp and Leptonema illini serovars using the microscopic agglutination test (MAT). To attempt isolation, urine samples from all animals were inoculated in culture medium.EMJH enriched with 10% of supplement Difco® One pathogenic serovar was isolated after two months of inoculation, and is first isolate the country for this animal species and was characterized by PCR using primers for 16S and Lipl32 genes. The technique of partial sequencing of the rpoB gene was used to identify the genomic specie. The isolated strain belongs to the specie L. borgpetersenii, which along with L .interrogans are the main cause disease in humans. The isolate, was tested on MAT using dog, cattle and human sera. Cut off titer of 50 was used for domestic animal sera and 25 for human and opossum sera. Both human and animal sera presented agglutination on MAT to isolated strain corresponding to 3.3% (2/60), 28.33% (17/60) and 1.67% (1/60) in human, dog and cattle sera, respectively. The opossum sera presented 36.36% (12/33) of reaction. These findings suggest a probable white-eared opossum role in the maintenance of pathogenic Leptospira on the environment since low titer sera and urine elimination were observed. These animals could be important reservoirs of Leptospira serovars for human and domestic animals. |
publishDate |
2009 |
dc.date.available.fl_str_mv |
2009-10-27 2014-08-20T14:37:58Z |
dc.date.issued.fl_str_mv |
2009-07-20 |
dc.date.accessioned.fl_str_mv |
2014-08-20T14:37:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
JORGE, Sérgio. Molecular identification and serological profile of Leptospira spp. isolated of white-eared-opossum (Didelphis albiventris) in South of Brazil. 2009. 57 f. Dissertação (Mestrado em Veterinária) - Universidade Federal de Pelotas, Pelotas, 2009. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufpel.edu.br/handle/ri/2539 |
identifier_str_mv |
JORGE, Sérgio. Molecular identification and serological profile of Leptospira spp. isolated of white-eared-opossum (Didelphis albiventris) in South of Brazil. 2009. 57 f. Dissertação (Mestrado em Veterinária) - Universidade Federal de Pelotas, Pelotas, 2009. |
url |
http://repositorio.ufpel.edu.br/handle/ri/2539 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Pelotas |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Veterinária |
dc.publisher.initials.fl_str_mv |
UFPel |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Pelotas |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFPel - Guaiaca instname:Universidade Federal de Pelotas (UFPEL) instacron:UFPEL |
instname_str |
Universidade Federal de Pelotas (UFPEL) |
instacron_str |
UFPEL |
institution |
UFPEL |
reponame_str |
Repositório Institucional da UFPel - Guaiaca |
collection |
Repositório Institucional da UFPel - Guaiaca |
bitstream.url.fl_str_mv |
http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2539/1/dissertacao_sergio_jorge.pdf http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2539/2/dissertacao_sergio_jorge.pdf.txt http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2539/3/dissertacao_sergio_jorge.pdf.jpg |
bitstream.checksum.fl_str_mv |
9e56cf9be902454868ace419c988ca33 4e9cc8a7d5c5d3a4297ed9c116076933 011437cd7f5bc918938491dca2e665df |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFPel - Guaiaca - Universidade Federal de Pelotas (UFPEL) |
repository.mail.fl_str_mv |
rippel@ufpel.edu.br || repositorio@ufpel.edu.br || aline.batista@ufpel.edu.br |
_version_ |
1797770016972603392 |