Metabolismo Antioxidativo para a classificação de lotes de sementes quanto ao vigor
Ano de defesa: | 2013 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Pelotas
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Fisiologia Vegetal
|
Departamento: |
Biologia
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufpel.edu.br/handle/ri/1998 |
Resumo: | The increased demand for high quality seeds led seed technology companies to improve their techniques aiming to associate germination and vigor with the responses of the seeds in the field. However, such responses may suffer several influences during storage and maturation process, bringing damage to the membranes' systems, leading to reactive oxygen species (ROS), which can diminish seed vigor. Thus, the antioxidative system enzymes may become an enriching alternative to classify seeds lots according to their vigor. Most works analyzing those enzymes activity report their responses related to the abiotic stress influence in different crops. In such cases, the decrease in the enzymatic activity point to seeds and seedlings with less vigor and viability. However, in this work, where the seeds were not submitted to any kind of stress, those enzymes higher activity expressed a higher ROS production, and that points to the beginning of the deterioration process, featuring lesser seeds vigor. Considering this, the work aimed to distinguish seeds lots of maize, beans and soybean through the antioxidative enzymes activity (SOD superoxide dismutase, APX ascorbate peroxidase and CAT - catalase), and relate them to viability and vigor standard test to verify if those enzymes detect differences in vigor of seeds lots. For this, the seeds of the three crops were submitted to the physiological quality standard tests, such as germination, first count and germination speed index, length and dry mass of roots and shoots, electrical conductivity and antioxidative enzymes. Through the obtained results, it was possible to verify that in beans and soybean seeds lots, which presented high heterogeneity between them, the antioxidative system enzymes activity was not efficient to separate the lots. However, in the maize seeds lots that presented high homogeneity for the physiological quality standard tests, it was possible identifying differences in the enzymes activity in the seedlings derived from those seeds lots, allowing to classify them into different vigor levels. |
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2014-08-20T13:59:06Z2013-07-052014-08-20T13:59:06Z2013-05-24BORBA, Isabel Cristina Gouvêa de. Antioxidative metabolism to classify cultivated species seeds lots according to vigor. 2013. 80 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Pelotas, Pelotas, 2013.http://repositorio.ufpel.edu.br/handle/ri/1998The increased demand for high quality seeds led seed technology companies to improve their techniques aiming to associate germination and vigor with the responses of the seeds in the field. However, such responses may suffer several influences during storage and maturation process, bringing damage to the membranes' systems, leading to reactive oxygen species (ROS), which can diminish seed vigor. Thus, the antioxidative system enzymes may become an enriching alternative to classify seeds lots according to their vigor. Most works analyzing those enzymes activity report their responses related to the abiotic stress influence in different crops. In such cases, the decrease in the enzymatic activity point to seeds and seedlings with less vigor and viability. However, in this work, where the seeds were not submitted to any kind of stress, those enzymes higher activity expressed a higher ROS production, and that points to the beginning of the deterioration process, featuring lesser seeds vigor. Considering this, the work aimed to distinguish seeds lots of maize, beans and soybean through the antioxidative enzymes activity (SOD superoxide dismutase, APX ascorbate peroxidase and CAT - catalase), and relate them to viability and vigor standard test to verify if those enzymes detect differences in vigor of seeds lots. For this, the seeds of the three crops were submitted to the physiological quality standard tests, such as germination, first count and germination speed index, length and dry mass of roots and shoots, electrical conductivity and antioxidative enzymes. Through the obtained results, it was possible to verify that in beans and soybean seeds lots, which presented high heterogeneity between them, the antioxidative system enzymes activity was not efficient to separate the lots. However, in the maize seeds lots that presented high homogeneity for the physiological quality standard tests, it was possible identifying differences in the enzymes activity in the seedlings derived from those seeds lots, allowing to classify them into different vigor levels.O aumento da demanda por sementes de alta qualidade levou as empresas de tecnologia de sementes a aprimorarem suas técnicas visando associar os testes de geminação e vigor com as respostas das sementes no campo. Contudo, tais respostas podem ser influenciadas durante o processo de maturação e armazenamento, acarretando danos aos sistemas de membranas e levando a formação de espécies reativas de oxigênio (EROs), as quais podem levar a diminuição do vigor das sementes. Deste modo, a avaliação das enzimas do sistema antioxidativo pode se tornar uma alternativa enriquecedora para classificar lotes de sementes quanto ao vigor. A maioria dos trabalhos que analisam a atividade dessas enzimas relacionam suas respostas frente a influência de estresses abióticos em diferentes culturas, nesses casos o decréscimo da atividade enzimática remete sementes e plântulas de menor viabilidade e vigor, contudo, neste trabalho onde as sementes não foram submetidas a nenhum tipo de estresse a maior atividade dessas enzimas representaria a maior produção de EROs que, consequentemente, evidencia o início do processo deteriorativo, caracterizando o menor vigor das sementes. Diante disso, neste trabalho o objetivo neste trabalho foi diferenciar lotes de sementes de milho, feijão e soja por meio da atividade das enzimas antioxidantes (SOD superóxido dismutase, APX ascorbato peroxidase e CAT catalase) e verificar se estas enzimas detectam pequenas diferenças no vigor das sementes. Para isso, as sementes das três culturas foram submetidas aos testes padrão de qualidade fisiológica, como germinação, primeira contagem e índice de velocidade de germinação, comprimento e massa seca da parte aérea e raízes, condutividade elétrica e posteriormente, submetidas à análise da atividade de enzimas antioxidantes. Através dos resultados obtidos foi possível verificar que nos lotes das sementes de feijão e soja, que apresentaram alta heterogeneidade entre eles a atividade das enzimas do sistema antioxidativo não foi eficiente na separação dos lotes, contudo, nos lotes de sementes de milho que apresentaram elevada homogeneidade pelos testes padrão de qualidade fisiológica foi possível evidenciar diferença na atividade das enzimas das plântulas oriundas desses lotes de sementes, permitindo classificar os mesmos em diferentes níveis de vigor.application/pdfporUniversidade Federal de PelotasPrograma de Pós-Graduação em Fisiologia VegetalUFPelBRBiologiaZea mays L.Phaseolus vulgaris L.Glycine max L.Enzimas antioxidantesQualidade fisiológicaZea mays L.Phaseolus vulgaris L.Glycine max L.Antioxidant enzymesPhysiological qualityCNPQ::CIENCIAS BIOLOGICAS::BOTANICA::FISIOLOGIA VEGETALMetabolismo Antioxidativo para a classificação de lotes de sementes quanto ao vigorAntioxidative metabolism to classify cultivated species seeds lots according to vigorinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttp://lattes.cnpq.br/0233191758488472http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787491U0Moraes, Dario Munt deBorba, Isabel Cristina Gouvêa deinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPel - Guaiacainstname:Universidade Federal de Pelotas (UFPEL)instacron:UFPELORIGINALdissertacao_isabel_borba.pdfapplication/pdf1492179http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1998/1/dissertacao_isabel_borba.pdf14d2b7301ebf251a8ecda34f71400f7aMD51open accessTEXTdissertacao_isabel_borba.pdf.txtdissertacao_isabel_borba.pdf.txtExtracted Texttext/plain122294http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1998/2/dissertacao_isabel_borba.pdf.txt361bbdbf2815beb0c97d1b1af47da304MD52open accessTHUMBNAILdissertacao_isabel_borba.pdf.jpgdissertacao_isabel_borba.pdf.jpgGenerated Thumbnailimage/jpeg1382http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1998/3/dissertacao_isabel_borba.pdf.jpgab64de7ce50e99e73e514a9ceaab73ffMD53open access123456789/19982019-09-19 08:56:16.9open accessoai:guaiaca.ufpel.edu.br:123456789/1998Repositório InstitucionalPUBhttp://repositorio.ufpel.edu.br/oai/requestrippel@ufpel.edu.br || repositorio@ufpel.edu.br || aline.batista@ufpel.edu.bropendoar:2019-09-19T11:56:16Repositório Institucional da UFPel - Guaiaca - Universidade Federal de Pelotas (UFPEL)false |
dc.title.por.fl_str_mv |
Metabolismo Antioxidativo para a classificação de lotes de sementes quanto ao vigor |
dc.title.alternative.eng.fl_str_mv |
Antioxidative metabolism to classify cultivated species seeds lots according to vigor |
title |
Metabolismo Antioxidativo para a classificação de lotes de sementes quanto ao vigor |
spellingShingle |
Metabolismo Antioxidativo para a classificação de lotes de sementes quanto ao vigor Borba, Isabel Cristina Gouvêa de Zea mays L. Phaseolus vulgaris L. Glycine max L. Enzimas antioxidantes Qualidade fisiológica Zea mays L. Phaseolus vulgaris L. Glycine max L. Antioxidant enzymes Physiological quality CNPQ::CIENCIAS BIOLOGICAS::BOTANICA::FISIOLOGIA VEGETAL |
title_short |
Metabolismo Antioxidativo para a classificação de lotes de sementes quanto ao vigor |
title_full |
Metabolismo Antioxidativo para a classificação de lotes de sementes quanto ao vigor |
title_fullStr |
Metabolismo Antioxidativo para a classificação de lotes de sementes quanto ao vigor |
title_full_unstemmed |
Metabolismo Antioxidativo para a classificação de lotes de sementes quanto ao vigor |
title_sort |
Metabolismo Antioxidativo para a classificação de lotes de sementes quanto ao vigor |
author |
Borba, Isabel Cristina Gouvêa de |
author_facet |
Borba, Isabel Cristina Gouvêa de |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/0233191758488472 |
dc.contributor.advisorLattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787491U0 |
dc.contributor.advisor1.fl_str_mv |
Moraes, Dario Munt de |
dc.contributor.author.fl_str_mv |
Borba, Isabel Cristina Gouvêa de |
contributor_str_mv |
Moraes, Dario Munt de |
dc.subject.por.fl_str_mv |
Zea mays L. Phaseolus vulgaris L. Glycine max L. Enzimas antioxidantes Qualidade fisiológica |
topic |
Zea mays L. Phaseolus vulgaris L. Glycine max L. Enzimas antioxidantes Qualidade fisiológica Zea mays L. Phaseolus vulgaris L. Glycine max L. Antioxidant enzymes Physiological quality CNPQ::CIENCIAS BIOLOGICAS::BOTANICA::FISIOLOGIA VEGETAL |
dc.subject.eng.fl_str_mv |
Zea mays L. Phaseolus vulgaris L. Glycine max L. Antioxidant enzymes Physiological quality |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BOTANICA::FISIOLOGIA VEGETAL |
description |
The increased demand for high quality seeds led seed technology companies to improve their techniques aiming to associate germination and vigor with the responses of the seeds in the field. However, such responses may suffer several influences during storage and maturation process, bringing damage to the membranes' systems, leading to reactive oxygen species (ROS), which can diminish seed vigor. Thus, the antioxidative system enzymes may become an enriching alternative to classify seeds lots according to their vigor. Most works analyzing those enzymes activity report their responses related to the abiotic stress influence in different crops. In such cases, the decrease in the enzymatic activity point to seeds and seedlings with less vigor and viability. However, in this work, where the seeds were not submitted to any kind of stress, those enzymes higher activity expressed a higher ROS production, and that points to the beginning of the deterioration process, featuring lesser seeds vigor. Considering this, the work aimed to distinguish seeds lots of maize, beans and soybean through the antioxidative enzymes activity (SOD superoxide dismutase, APX ascorbate peroxidase and CAT - catalase), and relate them to viability and vigor standard test to verify if those enzymes detect differences in vigor of seeds lots. For this, the seeds of the three crops were submitted to the physiological quality standard tests, such as germination, first count and germination speed index, length and dry mass of roots and shoots, electrical conductivity and antioxidative enzymes. Through the obtained results, it was possible to verify that in beans and soybean seeds lots, which presented high heterogeneity between them, the antioxidative system enzymes activity was not efficient to separate the lots. However, in the maize seeds lots that presented high homogeneity for the physiological quality standard tests, it was possible identifying differences in the enzymes activity in the seedlings derived from those seeds lots, allowing to classify them into different vigor levels. |
publishDate |
2013 |
dc.date.available.fl_str_mv |
2013-07-05 2014-08-20T13:59:06Z |
dc.date.issued.fl_str_mv |
2013-05-24 |
dc.date.accessioned.fl_str_mv |
2014-08-20T13:59:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BORBA, Isabel Cristina Gouvêa de. Antioxidative metabolism to classify cultivated species seeds lots according to vigor. 2013. 80 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Pelotas, Pelotas, 2013. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufpel.edu.br/handle/ri/1998 |
identifier_str_mv |
BORBA, Isabel Cristina Gouvêa de. Antioxidative metabolism to classify cultivated species seeds lots according to vigor. 2013. 80 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Pelotas, Pelotas, 2013. |
url |
http://repositorio.ufpel.edu.br/handle/ri/1998 |
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Universidade Federal de Pelotas |
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Programa de Pós-Graduação em Fisiologia Vegetal |
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UFPel |
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BR |
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Biologia |
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Universidade Federal de Pelotas |
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