Caracterização da resistência do algodoeiro a Ramularia areola e variabilidade molecular do patógeno
Ano de defesa: | 2007 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal do Rio Grande do Norte
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Genética e Biologia Molecular
|
Departamento: |
Genética e Biologia Molecular
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | https://repositorio.ufrn.br/jspui/handle/123456789/16781 |
Resumo: | This research was conducted with the aim to study the genetic and pathogenic structure of Ramularia areola isolates collected in Brazil and to characterize the resistance response in cotton plants to ramularia spot. The genetic variability of 28 isolates of R. areola was studied using RAPD markers. The pathogenicity evaluation was realized by the inoculation of 6 isolates on cotton varieties Guazuncho-2 (Gossypium hirsutum) and VH8-4602 (Gossypium barbadense). The inheritance of disease resistance was studied using an artificially inoculated population of F2 individuals derived from the intercross of Guazuncho-2 (susceptible variety) end VH8-4602 (resistant variety), and also the parents and F1 individuals. Molecular polymorphism between the G. hisutum varieties DeltaOpal (suscetible) and CNPA CO-11612 (resistant) was estimated by 118 SSR and 24 AFLP markers. The parental genotypes Guazuncho-2 and VH8-4602 were selected for mapping, and then Recombinant Inbred Lines (RIL´s) derived from this crossing were evaluated with SSR 12 markers. The analysis of population structure of R. areola revealed that the three subpopulations were genetically simillar (Gst=0.18), and the isolates from Goiás and Minas Gerais were more similar to each other (0,92). This probability can be related to the relatively high gene flow among the three subpopulations (Nm=2.20). The isolates R. areola 9.1, from Minas Gerais State and 8.1 and 8.3 from Goiás State were the most aggressive ones to the susceptible variety Guazuncho-2. The variety VH8-4602 presented high level of resistance to ramularia spot. No differential interaction was observed between the pathogens and the analyzed varieties, and the resistance was classified as horizontal. The quantification of disease by number of necrotic lesions and number of spores in individual plants of F1 and F2 generations from the crossing between the varieties Guazuncho-2 and VH8-4602 presented continuous distribution, suggesting polygenic resistance. The resistance is probabilly recessive, since necrotic lesions and sporulation were observed on F1 plants. The molecular polymorphism between DeltaOpal e CNPA CO-11612 lineages was low (6%), then would be difficult to accomplish molecular mapping of disease resistance using this intercross. With the genotyping of the RIL s it was verified that 25% of the markers segregated in the proportions proposed by Mendel s Law and 75% of the studied markers presented segregation distortion in favor to the parental G. hirsutum. Both the low genetic variability of the pathogen and the number of resistance genes suggest that durable genetic resitance may be achieved |
id |
UFRN_2ef4c368d017c70f1a4e3bddf225a176 |
---|---|
oai_identifier_str |
oai:https://repositorio.ufrn.br:123456789/16781 |
network_acronym_str |
UFRN |
network_name_str |
Repositório Institucional da UFRN |
repository_id_str |
|
spelling |
Lucena, Valeska Silvahttp://lattes.cnpq.br/4490260435452349http://lattes.cnpq.br/1552220402943995Suassuna, Nelson Diashttp://lattes.cnpq.br/4344026717541727Giband, Marchttp://lattes.cnpq.br/0551184684899446Hoffmann, Lúcia Vieira2014-12-17T15:18:13Z2007-07-182014-12-17T15:18:13Z2007-02-23LUCENA, Valeska Silva. Characterization of cotton resistance to Ramularia areola and molecular variability of the pathogen. 2007. 78 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal do Rio Grande do Norte, Natal, 2007.https://repositorio.ufrn.br/jspui/handle/123456789/16781This research was conducted with the aim to study the genetic and pathogenic structure of Ramularia areola isolates collected in Brazil and to characterize the resistance response in cotton plants to ramularia spot. The genetic variability of 28 isolates of R. areola was studied using RAPD markers. The pathogenicity evaluation was realized by the inoculation of 6 isolates on cotton varieties Guazuncho-2 (Gossypium hirsutum) and VH8-4602 (Gossypium barbadense). The inheritance of disease resistance was studied using an artificially inoculated population of F2 individuals derived from the intercross of Guazuncho-2 (susceptible variety) end VH8-4602 (resistant variety), and also the parents and F1 individuals. Molecular polymorphism between the G. hisutum varieties DeltaOpal (suscetible) and CNPA CO-11612 (resistant) was estimated by 118 SSR and 24 AFLP markers. The parental genotypes Guazuncho-2 and VH8-4602 were selected for mapping, and then Recombinant Inbred Lines (RIL´s) derived from this crossing were evaluated with SSR 12 markers. The analysis of population structure of R. areola revealed that the three subpopulations were genetically simillar (Gst=0.18), and the isolates from Goiás and Minas Gerais were more similar to each other (0,92). This probability can be related to the relatively high gene flow among the three subpopulations (Nm=2.20). The isolates R. areola 9.1, from Minas Gerais State and 8.1 and 8.3 from Goiás State were the most aggressive ones to the susceptible variety Guazuncho-2. The variety VH8-4602 presented high level of resistance to ramularia spot. No differential interaction was observed between the pathogens and the analyzed varieties, and the resistance was classified as horizontal. The quantification of disease by number of necrotic lesions and number of spores in individual plants of F1 and F2 generations from the crossing between the varieties Guazuncho-2 and VH8-4602 presented continuous distribution, suggesting polygenic resistance. The resistance is probabilly recessive, since necrotic lesions and sporulation were observed on F1 plants. The molecular polymorphism between DeltaOpal e CNPA CO-11612 lineages was low (6%), then would be difficult to accomplish molecular mapping of disease resistance using this intercross. With the genotyping of the RIL s it was verified that 25% of the markers segregated in the proportions proposed by Mendel s Law and 75% of the studied markers presented segregation distortion in favor to the parental G. hirsutum. Both the low genetic variability of the pathogen and the number of resistance genes suggest that durable genetic resitance may be achievedEste estudo teve como objetivos estudar a estrutura genética e patogênica de isolados de Ramularia areola do Brasil e caracterizar a resposta de resistência no algodoeiro à mancha-de-ramulária. Foi avaliada a variabilidade genética de 28 isolados do patógeno, utilizando-se marcadores RAPD. A avaliação da patogenicidade de 6 desses isolados foi realizada após a inoculação nas variedades Guazuncho-2 (Gossypium hirsutum) suscetível e VH8-4602 (Gossypium barbadense) resistente à doença. A herança da resistência no algodoeiro à doença foi caracterizada pela medição de intensidade da doença nas variedades Guazuncho-2 (G. hirsutum), suscetível, e VH8-4602 (G. barbadense), resistente à doença, e nas gerações F1 e F2 provenientes do cruzamento entre elas. O polimorfismo molecular entre as linhagens de G. hirsutum DeltaOpal e CNPA CO11612, contrastantes fenotipicamente quanto à resistência à doença foi avaliado utilizando-se 118 marcadores SSR e 24 AFLP. Para mapeamento molecular dos genes de resistência, linhagens recombinantes RIL s derivadas do cruzamento entre Guazuncho-2 e VH8-4602 foram genotipadas utilizando marcadores SSR. A análise da estrutura da população de R. areola revelou que as três subpopulações foram semelhantes geneticamente (Gst=0.18). Os isolados de Goiás e Minas Gerais obtiveram maior similaridade genética (0,92), que pode estar associada com o alto fluxo genético existente entre as subpopulações que foi de (Nm=2.20). Quanto à patogenicidade, os isolados de R. areola 9.1, coletado no estado de Minas Gerais, 8.2 e 8.3 de Goiás, foram mais agressivos na variedade suscetível Guazuncho-2. A variedade VH8-4602 apresentou alto nível de resistência à doença. Não foi observada interação diferencial entre os patógenos e as variedades analisadas, sendo a resistência classificada como horizontal. Na análise dos indivíduos das gerações F1 e F2, provenientes do cruzamento entre Guazuncho-2 e VH8-4602, a quantificação de sintomas da doença através da contagem de manchas necróticas e pelo número de esporos mostrou variação contínua da resistência à doença nos indivíduos da geração F2, indicando que a herança é poligênica. O aparecimento de sintomas e esporulação nos indivíduos F1 mostrou que a resistência é recessiva. O polimorfismo molecular entre as linhagens de G. hirsutum, DeltaOpal e CNPA CO11612 foi de 6%, portanto baixo, para realizar o mapeamento. Durante genotipagem das RIL´s foi verificado que 25% dos marcadores segregaram de acordo com as proporções das leis de Mendel, e 75% desses marcadores apresentaram distorção de segregação, com aumento da proporção de genes do parental G. hirsutum. A baixa variabilidade do patógeno e número de genes de resistência sugere que a resistência genética ao patógeno tenha alta durabilidadeapplication/pdfporUniversidade Federal do Rio Grande do NortePrograma de Pós-Graduação em Genética e Biologia MolecularUFRNBRGenética e Biologia MolecularGossypiumResistência genéticaMarcadores molecularesGossypium, Resistance geneticMarkers molecularCNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOSCaracterização da resistência do algodoeiro a Ramularia areola e variabilidade molecular do patógenoCharacterization of cotton resistance to Ramularia areola and molecular variability of the pathogeninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALValeskaSL.pdfapplication/pdf346083https://repositorio.ufrn.br/bitstream/123456789/16781/1/ValeskaSL.pdf77d65411fbea9e85070d5c90e9b52fefMD51TEXTValeskaSL.pdf.txtValeskaSL.pdf.txtExtracted texttext/plain129541https://repositorio.ufrn.br/bitstream/123456789/16781/6/ValeskaSL.pdf.txtaa6755d86009c1384199b7e0bced7452MD56THUMBNAILValeskaSL.pdf.jpgValeskaSL.pdf.jpgIM Thumbnailimage/jpeg3440https://repositorio.ufrn.br/bitstream/123456789/16781/7/ValeskaSL.pdf.jpg7e6fbb47b277ce252782092be8a7d3b9MD57123456789/167812017-11-04 05:51:04.522oai:https://repositorio.ufrn.br:123456789/16781Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2017-11-04T08:51:04Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.por.fl_str_mv |
Caracterização da resistência do algodoeiro a Ramularia areola e variabilidade molecular do patógeno |
dc.title.alternative.eng.fl_str_mv |
Characterization of cotton resistance to Ramularia areola and molecular variability of the pathogen |
title |
Caracterização da resistência do algodoeiro a Ramularia areola e variabilidade molecular do patógeno |
spellingShingle |
Caracterização da resistência do algodoeiro a Ramularia areola e variabilidade molecular do patógeno Lucena, Valeska Silva Gossypium Resistência genética Marcadores moleculares Gossypium, Resistance genetic Markers molecular CNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOS |
title_short |
Caracterização da resistência do algodoeiro a Ramularia areola e variabilidade molecular do patógeno |
title_full |
Caracterização da resistência do algodoeiro a Ramularia areola e variabilidade molecular do patógeno |
title_fullStr |
Caracterização da resistência do algodoeiro a Ramularia areola e variabilidade molecular do patógeno |
title_full_unstemmed |
Caracterização da resistência do algodoeiro a Ramularia areola e variabilidade molecular do patógeno |
title_sort |
Caracterização da resistência do algodoeiro a Ramularia areola e variabilidade molecular do patógeno |
author |
Lucena, Valeska Silva |
author_facet |
Lucena, Valeska Silva |
author_role |
author |
dc.contributor.authorID.por.fl_str_mv |
|
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/4490260435452349 |
dc.contributor.advisorID.por.fl_str_mv |
|
dc.contributor.advisorLattes.por.fl_str_mv |
http://lattes.cnpq.br/1552220402943995 |
dc.contributor.referees1.pt_BR.fl_str_mv |
Suassuna, Nelson Dias |
dc.contributor.referees1ID.por.fl_str_mv |
|
dc.contributor.referees1Lattes.por.fl_str_mv |
http://lattes.cnpq.br/4344026717541727 |
dc.contributor.referees2.pt_BR.fl_str_mv |
Giband, Marc |
dc.contributor.referees2ID.por.fl_str_mv |
|
dc.contributor.referees2Lattes.por.fl_str_mv |
http://lattes.cnpq.br/0551184684899446 |
dc.contributor.author.fl_str_mv |
Lucena, Valeska Silva |
dc.contributor.advisor1.fl_str_mv |
Hoffmann, Lúcia Vieira |
contributor_str_mv |
Hoffmann, Lúcia Vieira |
dc.subject.por.fl_str_mv |
Gossypium Resistência genética Marcadores moleculares |
topic |
Gossypium Resistência genética Marcadores moleculares Gossypium, Resistance genetic Markers molecular CNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOS |
dc.subject.eng.fl_str_mv |
Gossypium, Resistance genetic Markers molecular |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOS |
description |
This research was conducted with the aim to study the genetic and pathogenic structure of Ramularia areola isolates collected in Brazil and to characterize the resistance response in cotton plants to ramularia spot. The genetic variability of 28 isolates of R. areola was studied using RAPD markers. The pathogenicity evaluation was realized by the inoculation of 6 isolates on cotton varieties Guazuncho-2 (Gossypium hirsutum) and VH8-4602 (Gossypium barbadense). The inheritance of disease resistance was studied using an artificially inoculated population of F2 individuals derived from the intercross of Guazuncho-2 (susceptible variety) end VH8-4602 (resistant variety), and also the parents and F1 individuals. Molecular polymorphism between the G. hisutum varieties DeltaOpal (suscetible) and CNPA CO-11612 (resistant) was estimated by 118 SSR and 24 AFLP markers. The parental genotypes Guazuncho-2 and VH8-4602 were selected for mapping, and then Recombinant Inbred Lines (RIL´s) derived from this crossing were evaluated with SSR 12 markers. The analysis of population structure of R. areola revealed that the three subpopulations were genetically simillar (Gst=0.18), and the isolates from Goiás and Minas Gerais were more similar to each other (0,92). This probability can be related to the relatively high gene flow among the three subpopulations (Nm=2.20). The isolates R. areola 9.1, from Minas Gerais State and 8.1 and 8.3 from Goiás State were the most aggressive ones to the susceptible variety Guazuncho-2. The variety VH8-4602 presented high level of resistance to ramularia spot. No differential interaction was observed between the pathogens and the analyzed varieties, and the resistance was classified as horizontal. The quantification of disease by number of necrotic lesions and number of spores in individual plants of F1 and F2 generations from the crossing between the varieties Guazuncho-2 and VH8-4602 presented continuous distribution, suggesting polygenic resistance. The resistance is probabilly recessive, since necrotic lesions and sporulation were observed on F1 plants. The molecular polymorphism between DeltaOpal e CNPA CO-11612 lineages was low (6%), then would be difficult to accomplish molecular mapping of disease resistance using this intercross. With the genotyping of the RIL s it was verified that 25% of the markers segregated in the proportions proposed by Mendel s Law and 75% of the studied markers presented segregation distortion in favor to the parental G. hirsutum. Both the low genetic variability of the pathogen and the number of resistance genes suggest that durable genetic resitance may be achieved |
publishDate |
2007 |
dc.date.available.fl_str_mv |
2007-07-18 2014-12-17T15:18:13Z |
dc.date.issued.fl_str_mv |
2007-02-23 |
dc.date.accessioned.fl_str_mv |
2014-12-17T15:18:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
LUCENA, Valeska Silva. Characterization of cotton resistance to Ramularia areola and molecular variability of the pathogen. 2007. 78 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal do Rio Grande do Norte, Natal, 2007. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/jspui/handle/123456789/16781 |
identifier_str_mv |
LUCENA, Valeska Silva. Characterization of cotton resistance to Ramularia areola and molecular variability of the pathogen. 2007. 78 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal do Rio Grande do Norte, Natal, 2007. |
url |
https://repositorio.ufrn.br/jspui/handle/123456789/16781 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal do Rio Grande do Norte |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Genética e Biologia Molecular |
dc.publisher.initials.fl_str_mv |
UFRN |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Genética e Biologia Molecular |
publisher.none.fl_str_mv |
Universidade Federal do Rio Grande do Norte |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFRN instname:Universidade Federal do Rio Grande do Norte (UFRN) instacron:UFRN |
instname_str |
Universidade Federal do Rio Grande do Norte (UFRN) |
instacron_str |
UFRN |
institution |
UFRN |
reponame_str |
Repositório Institucional da UFRN |
collection |
Repositório Institucional da UFRN |
bitstream.url.fl_str_mv |
https://repositorio.ufrn.br/bitstream/123456789/16781/1/ValeskaSL.pdf https://repositorio.ufrn.br/bitstream/123456789/16781/6/ValeskaSL.pdf.txt https://repositorio.ufrn.br/bitstream/123456789/16781/7/ValeskaSL.pdf.jpg |
bitstream.checksum.fl_str_mv |
77d65411fbea9e85070d5c90e9b52fef aa6755d86009c1384199b7e0bced7452 7e6fbb47b277ce252782092be8a7d3b9 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN) |
repository.mail.fl_str_mv |
|
_version_ |
1796767055286894592 |