Avaliação da suplementação com vitamina E, na forma natural ou sintética, em mulheres no pós-parto imediato e sua concentração no colostro
Ano de defesa: | 2013 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal do Rio Grande do Norte
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Bioquímica
|
Departamento: |
Bioquímica; Biologia Molecular
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | https://repositorio.ufrn.br/jspui/handle/123456789/12617 |
Resumo: | The Vitamin E consists of eight chemically homologous forms, designated alpha, beta, gamma and delta tocopherols and tocotrienols. Biologically, the alpha-tocopherol (α-TOH) is the most important. Commercially, are found two types of α-TOH a natural (RRR-alpha-tocopherol) and another synthetic (all-rac-alpha-tocopherol). Both forms are absorbed in the intestine, the liver is a preference in favor of forms 2R, due to transfer protein α-TOH. It has higher affinity to these stereoisomers. Newborns are considered high risk for vitamin E deficiency, mainly premature, these have breast milk as a food source for maintenance of serum α-TOH. Clinical signs such as thrombocytosis, hemolytic anemia, retrolental fibroplasia, intraventricular hemorrhage, bronchopulmonary dysplasia and spinocerebellar degeneration can be found in case of a low intake of α-TOH. Thus, maternal supplementation on postpartum with α-TOH can be an efficient way to increase levels of vitamin E in breast milk and thus the consequently increase the supply of micronutrient for the newborn. However, most studies with vitamin E supplementation have been conducted in animals and little is known about the effect of maternal supplementation in humans, as well as on its efficiency to increase levels of α-TOH in human milk, depending on the shape natural or synthetic. The study included 109 women, divided into three groups: control without supplementation (GC) (n=36), supplemented with natural capsule (GNAT) (n=40) and the synthetic capsule (GSINT) (n=33). Blood samples were collected for determination of maternal nutritional status, and colostrums at initial contact and after 24 hours post-supplementation. Analyses were performed by High Performance Liquid Chromatography. Values of α-TOH in serum below 499.6mg/dL were considered deficient. We used the Kruskal-Wallis test and Tukey test to confirm the increase of alpha-tocopherol in milk and efficiency of administered capsules. Daily consumption of α-TOH was based on daily intake of 500 mL of colostrum by the newborn and compared with the nutritional requirement for children from 0 to 6 months of age, 4 mg / day. The mothers had mean concentration of serum α-TOH in 1016 ± 52, 1236 ± 51 and 1083 ± 61 mg / dL, in CG, GNAT and GSINT respectively. There were no women with deficiiency. The GC did not change the concentrations of α-TOH in colostrum. While women supplemented with natural and synthetic forms increased concentrations of α-TOH colostrum in 57.6% and 39%, respectively. By comparing supplemented groups, it was observed a significant difference (p=0.04), the natural capsule more efficient than the synthetic, approximately 49.6%. Individually, 21.1% of the women provided below 4mg/day of α-TOH, after supplementation for this index declined4.1%. Thus, maternal supplementation postpartum raised the levels of alpha-tocopherol in colostrum, and increased efficiency was observed with the natural form |
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Clemente, Heleni Aireshttp://lattes.cnpq.br/2608192490586369http://lattes.cnpq.br/8520928220989866Lima, João Paulo Matos Santoshttp://lattes.cnpq.br/3289758851760692Cerqueira, Mônica Maria Osório dehttp://lattes.cnpq.br/96713754613917652014-12-17T14:03:42Z2013-10-212014-12-17T14:03:42Z2013-06-10CLEMENTE, Heleni Aires. Avaliação da suplementação com vitamina E, na forma natural ou sintética, em mulheres no pós-parto imediato e sua concentração no colostro. 2013. 81 f. Dissertação (Mestrado em Bioquímica; Biologia Molecular) - Universidade Federal do Rio Grande do Norte, Natal, 2013.https://repositorio.ufrn.br/jspui/handle/123456789/12617The Vitamin E consists of eight chemically homologous forms, designated alpha, beta, gamma and delta tocopherols and tocotrienols. Biologically, the alpha-tocopherol (α-TOH) is the most important. Commercially, are found two types of α-TOH a natural (RRR-alpha-tocopherol) and another synthetic (all-rac-alpha-tocopherol). Both forms are absorbed in the intestine, the liver is a preference in favor of forms 2R, due to transfer protein α-TOH. It has higher affinity to these stereoisomers. Newborns are considered high risk for vitamin E deficiency, mainly premature, these have breast milk as a food source for maintenance of serum α-TOH. Clinical signs such as thrombocytosis, hemolytic anemia, retrolental fibroplasia, intraventricular hemorrhage, bronchopulmonary dysplasia and spinocerebellar degeneration can be found in case of a low intake of α-TOH. Thus, maternal supplementation on postpartum with α-TOH can be an efficient way to increase levels of vitamin E in breast milk and thus the consequently increase the supply of micronutrient for the newborn. However, most studies with vitamin E supplementation have been conducted in animals and little is known about the effect of maternal supplementation in humans, as well as on its efficiency to increase levels of α-TOH in human milk, depending on the shape natural or synthetic. The study included 109 women, divided into three groups: control without supplementation (GC) (n=36), supplemented with natural capsule (GNAT) (n=40) and the synthetic capsule (GSINT) (n=33). Blood samples were collected for determination of maternal nutritional status, and colostrums at initial contact and after 24 hours post-supplementation. Analyses were performed by High Performance Liquid Chromatography. Values of α-TOH in serum below 499.6mg/dL were considered deficient. We used the Kruskal-Wallis test and Tukey test to confirm the increase of alpha-tocopherol in milk and efficiency of administered capsules. Daily consumption of α-TOH was based on daily intake of 500 mL of colostrum by the newborn and compared with the nutritional requirement for children from 0 to 6 months of age, 4 mg / day. The mothers had mean concentration of serum α-TOH in 1016 ± 52, 1236 ± 51 and 1083 ± 61 mg / dL, in CG, GNAT and GSINT respectively. There were no women with deficiiency. The GC did not change the concentrations of α-TOH in colostrum. While women supplemented with natural and synthetic forms increased concentrations of α-TOH colostrum in 57.6% and 39%, respectively. By comparing supplemented groups, it was observed a significant difference (p=0.04), the natural capsule more efficient than the synthetic, approximately 49.6%. Individually, 21.1% of the women provided below 4mg/day of α-TOH, after supplementation for this index declined4.1%. Thus, maternal supplementation postpartum raised the levels of alpha-tocopherol in colostrum, and increased efficiency was observed with the natural formA vitamina E consiste em oito formas quimicamente homólogas, denominadas, alfa, beta, gama e delta, tocoferóis e tocotrienóis. Biologicamente, o alfa-tocoferol (α-TOH) é o mais importante. Comercialmente são encontradas duas formas de α-TOH, uma natural (RRR-alfa-tocoferol) e outra sintética (all-rac-alfa-tocoferol). Ambas as formas são absorvidas no intestino, entretanto, no fígado ocorre uma preferência em favor das formas 2R, devido à proteína de transferência de α-TOH, apresentar maior afinidade a estes estereoisômeros. Os neonatos são considerados grupo de risco para deficiência de vitamina E, principalmente os pré-maturos, estes têm o leite materno como a fonte alimentar para manutenção dos níveis séricos de α-TOH. Sinais clínicos como trombocitose, anemia hemolítica, fibroplasia retrolental, hemorragia intraventricular, displasia bronco pulmonar e degeneração espinocerebelar podem ser encontrados caso ocorra uma baixa ingestão de α-TOH. Sendo assim, a suplementação materna nos pós-parto com α-TOH pode ser uma forma eficiente de aumentar os níveis de vitamina E no leite materno e, consequentemente aumento no fornecimento do micronutriente para o recém-nascido. Entretanto, a maioria dos estudos com suplementação de vitamina E foram realizados em animais e são escassos os conhecimentos de sua suplementação em humanos, bem como, sobre sua eficiência para aumentar os níveis de α-TOH no leite humano, em função da forma natural ou sintética. Participaram do estudo 109 mulheres, distribuídas em três grupos: controle sem suplementação (GC) (n=36), suplementadas com a cápsula natural (GNAT) (n=40) e com a cápsula sintética (GSINT) (n=33). Foram coletadas amostras de sangue para determinação do estado nutricional materno, e de colostro no contato inicial e após 24 horas pós-suplementação. As análises foram realizadas por Cromatografia Líquida de Alta Eficiência. Valores de α-TOH no soro inferiores a 499,6 μg/dL foram considerados como deficientes. Foram utilizados o teste de Kruskal Wallis e teste de Tukey para confirmar o aumento de alfa-tocoferol no leite e a eficiência das cápsulas administradas. O consumo diário de α-TOH foi baseado na ingestão diária de 500 mL de colostro pelo recém-nascido e comparada com o requerimento nutricional para crianças de 0 à 6 meses de idade, 4 mg/dia. As parturientes apresentaram concentração média de α-TOH no soro de 1016 ± 52, 1236 ± 51 e 1083 ± 61 μg/dL, nos grupos GC, GNat e GSINT, respectivamente. Não foram encontradas mulheres com deficiência. O GC não apresentou variação nas concentrações de α-TOH no colostro. Enquanto mullheres suplementadas com as formas natural e sintética aumentaram as concentrações de α-TOH no colostro em 57,6% e 39%, respectivamente. Ao comparar os grupos suplementados foi observado uma diferença significativa (p=0,04), sendo a cápsula natural mais eficiente que a sintética em aproximadamente 49,6%. Individualmente 21,1% das mulheres forneceram valores inferiores as 4mg/dia de α-TOH, após a suplementação este índice declinou para 4,1%. Sendo assim, a suplementação materna no pós-parto elevou os níveis de alfa-tocoferol no colostro e maior eficiência foi observada com a forma naturalConselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal do Rio Grande do NortePrograma de Pós-Graduação em BioquímicaUFRNBRBioquímica; Biologia MolecularAlfa-tocoferolDeficiência de vitamina ELactantesRequerimento nutricionalCLAEAlpha-tocopherolVitamin E deficiencyLactatingNutritional RequirementHPLCCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAAvaliação da suplementação com vitamina E, na forma natural ou sintética, em mulheres no pós-parto imediato e sua concentração no colostroinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALAvaliacaoSuplementacaoVitaminaE_Clemente_2013.pdfapplication/pdf2512013https://repositorio.ufrn.br/bitstream/123456789/12617/1/AvaliacaoSuplementacaoVitaminaE_Clemente_2013.pdfe69e375e25ebb38b43385a02d5ecc6c7MD51TEXTHeleniAC_DISSERT.pdf.txtHeleniAC_DISSERT.pdf.txtExtracted texttext/plain125563https://repositorio.ufrn.br/bitstream/123456789/12617/6/HeleniAC_DISSERT.pdf.txt1845a7d896352140901a6d4e6148682bMD56AvaliacaoSuplementacaoVitaminaE_Clemente_2013.pdf.txtAvaliacaoSuplementacaoVitaminaE_Clemente_2013.pdf.txtExtracted texttext/plain125563https://repositorio.ufrn.br/bitstream/123456789/12617/8/AvaliacaoSuplementacaoVitaminaE_Clemente_2013.pdf.txt1845a7d896352140901a6d4e6148682bMD58THUMBNAILHeleniAC_DISSERT.pdf.jpgHeleniAC_DISSERT.pdf.jpgIM Thumbnailimage/jpeg3318https://repositorio.ufrn.br/bitstream/123456789/12617/7/HeleniAC_DISSERT.pdf.jpgb55a1ce4e0363a400a1fecd9574c6d87MD57AvaliacaoSuplementacaoVitaminaE_Clemente_2013.pdf.jpgAvaliacaoSuplementacaoVitaminaE_Clemente_2013.pdf.jpgIM Thumbnailimage/jpeg3318https://repositorio.ufrn.br/bitstream/123456789/12617/9/AvaliacaoSuplementacaoVitaminaE_Clemente_2013.pdf.jpgb55a1ce4e0363a400a1fecd9574c6d87MD59123456789/126172019-01-30 06:19:31.483oai:https://repositorio.ufrn.br:123456789/12617Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2019-01-30T09:19:31Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.por.fl_str_mv |
Avaliação da suplementação com vitamina E, na forma natural ou sintética, em mulheres no pós-parto imediato e sua concentração no colostro |
title |
Avaliação da suplementação com vitamina E, na forma natural ou sintética, em mulheres no pós-parto imediato e sua concentração no colostro |
spellingShingle |
Avaliação da suplementação com vitamina E, na forma natural ou sintética, em mulheres no pós-parto imediato e sua concentração no colostro Clemente, Heleni Aires Alfa-tocoferol Deficiência de vitamina E Lactantes Requerimento nutricional CLAE Alpha-tocopherol Vitamin E deficiency Lactating Nutritional Requirement HPLC CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Avaliação da suplementação com vitamina E, na forma natural ou sintética, em mulheres no pós-parto imediato e sua concentração no colostro |
title_full |
Avaliação da suplementação com vitamina E, na forma natural ou sintética, em mulheres no pós-parto imediato e sua concentração no colostro |
title_fullStr |
Avaliação da suplementação com vitamina E, na forma natural ou sintética, em mulheres no pós-parto imediato e sua concentração no colostro |
title_full_unstemmed |
Avaliação da suplementação com vitamina E, na forma natural ou sintética, em mulheres no pós-parto imediato e sua concentração no colostro |
title_sort |
Avaliação da suplementação com vitamina E, na forma natural ou sintética, em mulheres no pós-parto imediato e sua concentração no colostro |
author |
Clemente, Heleni Aires |
author_facet |
Clemente, Heleni Aires |
author_role |
author |
dc.contributor.authorID.por.fl_str_mv |
|
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/2608192490586369 |
dc.contributor.advisorID.por.fl_str_mv |
|
dc.contributor.advisorLattes.por.fl_str_mv |
http://lattes.cnpq.br/8520928220989866 |
dc.contributor.referees1.pt_BR.fl_str_mv |
Lima, João Paulo Matos Santos |
dc.contributor.referees1ID.por.fl_str_mv |
|
dc.contributor.referees1Lattes.por.fl_str_mv |
http://lattes.cnpq.br/3289758851760692 |
dc.contributor.referees2.pt_BR.fl_str_mv |
Cerqueira, Mônica Maria Osório de |
dc.contributor.referees2ID.por.fl_str_mv |
|
dc.contributor.referees2Lattes.por.fl_str_mv |
http://lattes.cnpq.br/9671375461391765 |
dc.contributor.author.fl_str_mv |
Clemente, Heleni Aires |
dc.subject.por.fl_str_mv |
Alfa-tocoferol Deficiência de vitamina E Lactantes Requerimento nutricional CLAE |
topic |
Alfa-tocoferol Deficiência de vitamina E Lactantes Requerimento nutricional CLAE Alpha-tocopherol Vitamin E deficiency Lactating Nutritional Requirement HPLC CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.eng.fl_str_mv |
Alpha-tocopherol Vitamin E deficiency Lactating Nutritional Requirement HPLC |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
The Vitamin E consists of eight chemically homologous forms, designated alpha, beta, gamma and delta tocopherols and tocotrienols. Biologically, the alpha-tocopherol (α-TOH) is the most important. Commercially, are found two types of α-TOH a natural (RRR-alpha-tocopherol) and another synthetic (all-rac-alpha-tocopherol). Both forms are absorbed in the intestine, the liver is a preference in favor of forms 2R, due to transfer protein α-TOH. It has higher affinity to these stereoisomers. Newborns are considered high risk for vitamin E deficiency, mainly premature, these have breast milk as a food source for maintenance of serum α-TOH. Clinical signs such as thrombocytosis, hemolytic anemia, retrolental fibroplasia, intraventricular hemorrhage, bronchopulmonary dysplasia and spinocerebellar degeneration can be found in case of a low intake of α-TOH. Thus, maternal supplementation on postpartum with α-TOH can be an efficient way to increase levels of vitamin E in breast milk and thus the consequently increase the supply of micronutrient for the newborn. However, most studies with vitamin E supplementation have been conducted in animals and little is known about the effect of maternal supplementation in humans, as well as on its efficiency to increase levels of α-TOH in human milk, depending on the shape natural or synthetic. The study included 109 women, divided into three groups: control without supplementation (GC) (n=36), supplemented with natural capsule (GNAT) (n=40) and the synthetic capsule (GSINT) (n=33). Blood samples were collected for determination of maternal nutritional status, and colostrums at initial contact and after 24 hours post-supplementation. Analyses were performed by High Performance Liquid Chromatography. Values of α-TOH in serum below 499.6mg/dL were considered deficient. We used the Kruskal-Wallis test and Tukey test to confirm the increase of alpha-tocopherol in milk and efficiency of administered capsules. Daily consumption of α-TOH was based on daily intake of 500 mL of colostrum by the newborn and compared with the nutritional requirement for children from 0 to 6 months of age, 4 mg / day. The mothers had mean concentration of serum α-TOH in 1016 ± 52, 1236 ± 51 and 1083 ± 61 mg / dL, in CG, GNAT and GSINT respectively. There were no women with deficiiency. The GC did not change the concentrations of α-TOH in colostrum. While women supplemented with natural and synthetic forms increased concentrations of α-TOH colostrum in 57.6% and 39%, respectively. By comparing supplemented groups, it was observed a significant difference (p=0.04), the natural capsule more efficient than the synthetic, approximately 49.6%. Individually, 21.1% of the women provided below 4mg/day of α-TOH, after supplementation for this index declined4.1%. Thus, maternal supplementation postpartum raised the levels of alpha-tocopherol in colostrum, and increased efficiency was observed with the natural form |
publishDate |
2013 |
dc.date.available.fl_str_mv |
2013-10-21 2014-12-17T14:03:42Z |
dc.date.issued.fl_str_mv |
2013-06-10 |
dc.date.accessioned.fl_str_mv |
2014-12-17T14:03:42Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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publishedVersion |
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CLEMENTE, Heleni Aires. Avaliação da suplementação com vitamina E, na forma natural ou sintética, em mulheres no pós-parto imediato e sua concentração no colostro. 2013. 81 f. Dissertação (Mestrado em Bioquímica; Biologia Molecular) - Universidade Federal do Rio Grande do Norte, Natal, 2013. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/jspui/handle/123456789/12617 |
identifier_str_mv |
CLEMENTE, Heleni Aires. Avaliação da suplementação com vitamina E, na forma natural ou sintética, em mulheres no pós-parto imediato e sua concentração no colostro. 2013. 81 f. Dissertação (Mestrado em Bioquímica; Biologia Molecular) - Universidade Federal do Rio Grande do Norte, Natal, 2013. |
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https://repositorio.ufrn.br/jspui/handle/123456789/12617 |
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Universidade Federal do Rio Grande do Norte |
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Programa de Pós-Graduação em Bioquímica |
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UFRN |
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BR |
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Bioquímica; Biologia Molecular |
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Universidade Federal do Rio Grande do Norte |
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