Estudo integrado do potencial fitorremediador da Eichhornia crassipes em ambientes naturais e sua utilização para obtenção de extratos proteicos
Ano de defesa: | 2014 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal do Rio Grande do Norte
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Química
|
Departamento: |
Físico-Química; Química
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | https://repositorio.ufrn.br/jspui/handle/123456789/17791 |
Resumo: | This study aimed to analyze the phytoremediation potential of Eichhornia crassipes in natural environments, optimize the extraction process of crude protein from plant tissue and, obtain and characterize this process in order to determine its viability of use instead of the protein sources of animal and/or human feed. For this, it has been determined in Apodi/Mossoró river water the concentration of ammonium ions, nitrite, nitrate, calcium, magnesium, potassium, iron, copper, manganese, zinc, nickel, cobalt, sodium, aluminum, cádmium, lead, and total chromium; It was determined in plant tissue of aquatic macrophytes of Eichhornia crassipes species present in Apodi/Mossoró River the moisture content, ash, calcium, magnesium, potassium, iron, copper, manganese, zinc, nickel, cobalt, sodium, aluminum, cadmium, lead, total chromium, total nitrogen and crude protein. It was also determined the translocation factor and bioaccumulation of all the quantified elements; It was developed and optimized the extraction procedure of crude protein based on the isoelectric method and a factorial design 24 with repetition; It was extracted and characterized the extract obtained by determining the moisture content, ash, magnesium, potassium, iron, copper, manganese, zinc, nickel, cobalt, sodium, cadmium, total nitrogen and crude protein. And finally, it was also characterized the protein extract using Thermogravimetric Analysis (TG), Derived Thermogravimetric (DTG), Differential Scanning Calorimetry (DSC), Infrared Spectroscopy (FT-IR) and jelly-like electrophoresis of polyacrylamide (SDS -PAGE) to assess the their molecular weights/mass. Thus, from the results obtained for the translocation and bioaccumulation factors was found that the same can be used as phytoremediation agent in natural environments of all quantified elements. It was also found that the developed method of extraction and protein precipitation was satisfactory for the purpose of the work, which gave the best conditions of extraction and precipitation of proteins as: pH extraction equal to 13.0, extraction temperature equals 60 ° C, reaction time equals to 30 minutes, and pH precipitation equals to 4.0. As for the extract obtained, the total nitrogen and crude protein were quantified higher than those found in the plant, increasing the crude protein content approximately 116.88% in relation to the quantified contente in the vegetal tissue of macrophyte. The levels of nickel and cadmium were the unique that were found below the detection limit of used the equipment. The electrophoretic analysis allowed us to observe that the protein extract obtained is composed of low polypeptide chains by the molecular and phytochelatins, with 6 and 15 kDa bands. Analysis of TG, DTG, DSC and FT-IR showed similarities in protein content of the obtained extracts based on different collection points and 9 parts of the plant under study, as well as commercial soy protein and casein. Finally, based on all these findings, it was concluded that the obtained extract in this work can be used instead of the protein sources of animal feed should, before that, test its digestibility. As human supplementation, it is necessary to conduct more tests associated with the optimization process in the sense of removing undesirable components and constant monitoring of the water body and the raw material used |
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Martins, Daniel Freitas Freirehttp://lattes.cnpq.br/1834691679987332http://lattes.cnpq.br/2959800336802498Santos, Klecia Morais doshttp://lattes.cnpq.br/7924455214362992Souza, Luiz Dihttp://lattes.cnpq.br/0589648773241934Fernandes, Nedja Suelyhttp://lattes.cnpq.br/9563490368583906Batista, Rafael Oliveirahttp://lattes.cnpq.br/6747258062404427Castro, Suely Souza Leal dehttp://lattes.cnpq.br/9860381828526444Moura, Maria de Fátima Vitória de2014-12-17T15:42:29Z2014-07-212014-12-17T15:42:29Z2014-02-24MARTINS, Daniel Freitas Freire. Estudo integrado do potencial fitorremediador da Eichhornia crassipes em ambientes naturais e sua utilização para obtenção de extratos proteicos. 2014. 164 f. Tese (Doutorado em Físico-Química; Química) - Universidade Federal do Rio Grande do Norte, Natal, 2014.https://repositorio.ufrn.br/jspui/handle/123456789/17791This study aimed to analyze the phytoremediation potential of Eichhornia crassipes in natural environments, optimize the extraction process of crude protein from plant tissue and, obtain and characterize this process in order to determine its viability of use instead of the protein sources of animal and/or human feed. For this, it has been determined in Apodi/Mossoró river water the concentration of ammonium ions, nitrite, nitrate, calcium, magnesium, potassium, iron, copper, manganese, zinc, nickel, cobalt, sodium, aluminum, cádmium, lead, and total chromium; It was determined in plant tissue of aquatic macrophytes of Eichhornia crassipes species present in Apodi/Mossoró River the moisture content, ash, calcium, magnesium, potassium, iron, copper, manganese, zinc, nickel, cobalt, sodium, aluminum, cadmium, lead, total chromium, total nitrogen and crude protein. It was also determined the translocation factor and bioaccumulation of all the quantified elements; It was developed and optimized the extraction procedure of crude protein based on the isoelectric method and a factorial design 24 with repetition; It was extracted and characterized the extract obtained by determining the moisture content, ash, magnesium, potassium, iron, copper, manganese, zinc, nickel, cobalt, sodium, cadmium, total nitrogen and crude protein. And finally, it was also characterized the protein extract using Thermogravimetric Analysis (TG), Derived Thermogravimetric (DTG), Differential Scanning Calorimetry (DSC), Infrared Spectroscopy (FT-IR) and jelly-like electrophoresis of polyacrylamide (SDS -PAGE) to assess the their molecular weights/mass. Thus, from the results obtained for the translocation and bioaccumulation factors was found that the same can be used as phytoremediation agent in natural environments of all quantified elements. It was also found that the developed method of extraction and protein precipitation was satisfactory for the purpose of the work, which gave the best conditions of extraction and precipitation of proteins as: pH extraction equal to 13.0, extraction temperature equals 60 ° C, reaction time equals to 30 minutes, and pH precipitation equals to 4.0. As for the extract obtained, the total nitrogen and crude protein were quantified higher than those found in the plant, increasing the crude protein content approximately 116.88% in relation to the quantified contente in the vegetal tissue of macrophyte. The levels of nickel and cadmium were the unique that were found below the detection limit of used the equipment. The electrophoretic analysis allowed us to observe that the protein extract obtained is composed of low polypeptide chains by the molecular and phytochelatins, with 6 and 15 kDa bands. Analysis of TG, DTG, DSC and FT-IR showed similarities in protein content of the obtained extracts based on different collection points and 9 parts of the plant under study, as well as commercial soy protein and casein. Finally, based on all these findings, it was concluded that the obtained extract in this work can be used instead of the protein sources of animal feed should, before that, test its digestibility. As human supplementation, it is necessary to conduct more tests associated with the optimization process in the sense of removing undesirable components and constant monitoring of the water body and the raw material usedEste trabalho analisou o potencial fitorremediador da Eichhornia crassipes em ambientes naturais, otimizou o processo de extração da proteína bruta do tecido vegetal e obteve e caracterizou a mesma, determinando sua viabilidade de uso em substituição as fontes protéicas de rações animais e/ou humana. Para isso, determinou-se na água do Rio Apodi/Mossoró a concentração de íons amônio, nitrito, nitrato, cálcio, magnésio, potássio, ferro, cobre, manganês, zinco, níquel, cobalto, sódio, alumínio, cádmio, chumbo e cromo total; Determinou-se no tecido vegetal das macrófitas aquáticas da espécie Eichhornia crassipes presentes no Rio Apodi/Mossoró o teor de umidade, cinzas, nitrogênio total e proteína bruta e os mesmos metais determinados na água. Calculou-se o fator de translocação e bioacumulação de todos os elementos quantificados; Desenvolveu-se e otimizou-se o procedimento de extração da proteína bruta utilizando como base o método isoelétrico e um planejamento fatorial 24 com repetição; Extraiu-se e caracterizou-se o extrato obtido através da determinação o teor de umidade, cinzas, nitrogênio total e proteína bruta e os mesmos metais determinados na água. E, por fim, caracterizou-se também o extrato proteico utilizando Análise Termogravimétrica (TG), Termogravimetria derivada (DTG), Calorimetria Exploratória Diferencial (DSC), Espectroscopia de Infravermelho (FT-IR) e Eletroforese em gel de poliacrilamida (SDS-PAGE) para avaliar as suas massas molares. Desta forma, a partir dos resultados obtidos para os fatores de translocação e bioacumulação constatou-se que a mesma pode ser utilizada como agente fitorremediador em ambientes naturais de todos os elementos quantificados. Constatou-se ainda que o método desenvolvido de extração e precipitação de proteínas foi satisfatório para o objetivo do trabalho, onde obteve-se as melhores condições de extração e precipitação das proteínas como sendo: pH de extração igual a 13,0; temperatura de extração igual a 60°C; tempo de reação igual a 30 minutos; e pH de precipitação igual a 4,0. Quanto ao extrato obtido, os teores de nitrogênio total e proteína bruta quantificados foram superiores aos encontrados na planta, chegando a aumentar o teor de proteína bruta cerca de 116,88% em relação ao teor quantificado no tecido vegetal da macrófita. Os teores de níquel e o cádmio foram os únicos que se apresentaram abaixo do limite de detecção do equipamento utilizado. A análise eletroforética permitiu observar que o extrato proteico obtido é constituído de cadeias polipeptídicas de baixo peso molecular e fitoquelatinas, com bandas de 6 e 15 kDa. As análises de TG, DTG, DSC e FT-IR permitiram constatar semelhanças existentes no conteúdo proteico dos extratos obtidos a partir de diferentes pontos de coleta e partes da planta em estudo, bem como da proteína de soja 7 comercial e da caseína. Por fim, com base em todas essas constatações, conclui-se que o extrato obtido no presente trabalho pode ser utilizado em substituição às fontes proteicas de rações animais devendo, antes disso, ser testado a sua digestibilidade. Quanto a suplementação humana, é preciso a realização de mais testes associados a otimização do processo no sentido de remoção dos componentes indesejáveis e constante monitoramento do corpo hídrico e da matéria-prima utilizadaConselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal do Rio Grande do NortePrograma de Pós-Graduação em QuímicaUFRNBRFísico-Química; QuímicaExtrato proteico. Macrófita. Fitorremediação. Rio Apodi/Mossoró.Protein extract. Macrophyte. Phytoremediation. River Apodi/Mossoró.CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICAEstudo integrado do potencial fitorremediador da Eichhornia crassipes em ambientes naturais e sua utilização para obtenção de extratos proteicosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALDanielFFM_TESE.pdfapplication/pdf2565491https://repositorio.ufrn.br/bitstream/123456789/17791/1/DanielFFM_TESE.pdf91650e117d4deece934978a16f9a23d9MD51TEXTDanielFFM_TESE.pdf.txtDanielFFM_TESE.pdf.txtExtracted texttext/plain247259https://repositorio.ufrn.br/bitstream/123456789/17791/6/DanielFFM_TESE.pdf.txt8e5b84311d34ebc81fb4e2d7aafd0c93MD56THUMBNAILDanielFFM_TESE.pdf.jpgDanielFFM_TESE.pdf.jpgIM Thumbnailimage/jpeg5621https://repositorio.ufrn.br/bitstream/123456789/17791/7/DanielFFM_TESE.pdf.jpg2eadd74bcbdddc9ec0a01ae9f9a4760dMD57123456789/177912017-11-04 15:27:59.639oai:https://repositorio.ufrn.br:123456789/17791Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2017-11-04T18:27:59Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.por.fl_str_mv |
Estudo integrado do potencial fitorremediador da Eichhornia crassipes em ambientes naturais e sua utilização para obtenção de extratos proteicos |
title |
Estudo integrado do potencial fitorremediador da Eichhornia crassipes em ambientes naturais e sua utilização para obtenção de extratos proteicos |
spellingShingle |
Estudo integrado do potencial fitorremediador da Eichhornia crassipes em ambientes naturais e sua utilização para obtenção de extratos proteicos Martins, Daniel Freitas Freire Extrato proteico. Macrófita. Fitorremediação. Rio Apodi/Mossoró. Protein extract. Macrophyte. Phytoremediation. River Apodi/Mossoró. CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
title_short |
Estudo integrado do potencial fitorremediador da Eichhornia crassipes em ambientes naturais e sua utilização para obtenção de extratos proteicos |
title_full |
Estudo integrado do potencial fitorremediador da Eichhornia crassipes em ambientes naturais e sua utilização para obtenção de extratos proteicos |
title_fullStr |
Estudo integrado do potencial fitorremediador da Eichhornia crassipes em ambientes naturais e sua utilização para obtenção de extratos proteicos |
title_full_unstemmed |
Estudo integrado do potencial fitorremediador da Eichhornia crassipes em ambientes naturais e sua utilização para obtenção de extratos proteicos |
title_sort |
Estudo integrado do potencial fitorremediador da Eichhornia crassipes em ambientes naturais e sua utilização para obtenção de extratos proteicos |
author |
Martins, Daniel Freitas Freire |
author_facet |
Martins, Daniel Freitas Freire |
author_role |
author |
dc.contributor.authorID.por.fl_str_mv |
|
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/1834691679987332 |
dc.contributor.advisorID.por.fl_str_mv |
|
dc.contributor.advisorLattes.por.fl_str_mv |
http://lattes.cnpq.br/2959800336802498 |
dc.contributor.referees1.pt_BR.fl_str_mv |
Santos, Klecia Morais dos |
dc.contributor.referees1ID.por.fl_str_mv |
|
dc.contributor.referees1Lattes.por.fl_str_mv |
http://lattes.cnpq.br/7924455214362992 |
dc.contributor.referees2.pt_BR.fl_str_mv |
Souza, Luiz Di |
dc.contributor.referees2ID.por.fl_str_mv |
|
dc.contributor.referees2Lattes.por.fl_str_mv |
http://lattes.cnpq.br/0589648773241934 |
dc.contributor.referees3.pt_BR.fl_str_mv |
Fernandes, Nedja Suely |
dc.contributor.referees3ID.por.fl_str_mv |
|
dc.contributor.referees3Lattes.por.fl_str_mv |
http://lattes.cnpq.br/9563490368583906 |
dc.contributor.referees4.pt_BR.fl_str_mv |
Batista, Rafael Oliveira |
dc.contributor.referees4ID.por.fl_str_mv |
|
dc.contributor.referees4Lattes.por.fl_str_mv |
http://lattes.cnpq.br/6747258062404427 |
dc.contributor.referees5.pt_BR.fl_str_mv |
Castro, Suely Souza Leal de |
dc.contributor.referees5ID.por.fl_str_mv |
|
dc.contributor.referees5Lattes.por.fl_str_mv |
http://lattes.cnpq.br/9860381828526444 |
dc.contributor.author.fl_str_mv |
Martins, Daniel Freitas Freire |
dc.contributor.advisor1.fl_str_mv |
Moura, Maria de Fátima Vitória de |
contributor_str_mv |
Moura, Maria de Fátima Vitória de |
dc.subject.por.fl_str_mv |
Extrato proteico. Macrófita. Fitorremediação. Rio Apodi/Mossoró. |
topic |
Extrato proteico. Macrófita. Fitorremediação. Rio Apodi/Mossoró. Protein extract. Macrophyte. Phytoremediation. River Apodi/Mossoró. CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
dc.subject.eng.fl_str_mv |
Protein extract. Macrophyte. Phytoremediation. River Apodi/Mossoró. |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
description |
This study aimed to analyze the phytoremediation potential of Eichhornia crassipes in natural environments, optimize the extraction process of crude protein from plant tissue and, obtain and characterize this process in order to determine its viability of use instead of the protein sources of animal and/or human feed. For this, it has been determined in Apodi/Mossoró river water the concentration of ammonium ions, nitrite, nitrate, calcium, magnesium, potassium, iron, copper, manganese, zinc, nickel, cobalt, sodium, aluminum, cádmium, lead, and total chromium; It was determined in plant tissue of aquatic macrophytes of Eichhornia crassipes species present in Apodi/Mossoró River the moisture content, ash, calcium, magnesium, potassium, iron, copper, manganese, zinc, nickel, cobalt, sodium, aluminum, cadmium, lead, total chromium, total nitrogen and crude protein. It was also determined the translocation factor and bioaccumulation of all the quantified elements; It was developed and optimized the extraction procedure of crude protein based on the isoelectric method and a factorial design 24 with repetition; It was extracted and characterized the extract obtained by determining the moisture content, ash, magnesium, potassium, iron, copper, manganese, zinc, nickel, cobalt, sodium, cadmium, total nitrogen and crude protein. And finally, it was also characterized the protein extract using Thermogravimetric Analysis (TG), Derived Thermogravimetric (DTG), Differential Scanning Calorimetry (DSC), Infrared Spectroscopy (FT-IR) and jelly-like electrophoresis of polyacrylamide (SDS -PAGE) to assess the their molecular weights/mass. Thus, from the results obtained for the translocation and bioaccumulation factors was found that the same can be used as phytoremediation agent in natural environments of all quantified elements. It was also found that the developed method of extraction and protein precipitation was satisfactory for the purpose of the work, which gave the best conditions of extraction and precipitation of proteins as: pH extraction equal to 13.0, extraction temperature equals 60 ° C, reaction time equals to 30 minutes, and pH precipitation equals to 4.0. As for the extract obtained, the total nitrogen and crude protein were quantified higher than those found in the plant, increasing the crude protein content approximately 116.88% in relation to the quantified contente in the vegetal tissue of macrophyte. The levels of nickel and cadmium were the unique that were found below the detection limit of used the equipment. The electrophoretic analysis allowed us to observe that the protein extract obtained is composed of low polypeptide chains by the molecular and phytochelatins, with 6 and 15 kDa bands. Analysis of TG, DTG, DSC and FT-IR showed similarities in protein content of the obtained extracts based on different collection points and 9 parts of the plant under study, as well as commercial soy protein and casein. Finally, based on all these findings, it was concluded that the obtained extract in this work can be used instead of the protein sources of animal feed should, before that, test its digestibility. As human supplementation, it is necessary to conduct more tests associated with the optimization process in the sense of removing undesirable components and constant monitoring of the water body and the raw material used |
publishDate |
2014 |
dc.date.accessioned.fl_str_mv |
2014-12-17T15:42:29Z |
dc.date.available.fl_str_mv |
2014-07-21 2014-12-17T15:42:29Z |
dc.date.issued.fl_str_mv |
2014-02-24 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MARTINS, Daniel Freitas Freire. Estudo integrado do potencial fitorremediador da Eichhornia crassipes em ambientes naturais e sua utilização para obtenção de extratos proteicos. 2014. 164 f. Tese (Doutorado em Físico-Química; Química) - Universidade Federal do Rio Grande do Norte, Natal, 2014. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/jspui/handle/123456789/17791 |
identifier_str_mv |
MARTINS, Daniel Freitas Freire. Estudo integrado do potencial fitorremediador da Eichhornia crassipes em ambientes naturais e sua utilização para obtenção de extratos proteicos. 2014. 164 f. Tese (Doutorado em Físico-Química; Química) - Universidade Federal do Rio Grande do Norte, Natal, 2014. |
url |
https://repositorio.ufrn.br/jspui/handle/123456789/17791 |
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por |
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Universidade Federal do Rio Grande do Norte |
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Programa de Pós-Graduação em Química |
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UFRN |
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BR |
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Físico-Química; Química |
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Universidade Federal do Rio Grande do Norte |
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