Caracteriza??o de estirpes de Bradyrhizobium isoladas de Centrolobium paraense

Detalhes bibliográficos
Ano de defesa: 2017
Autor(a) principal: Michel, Daniele Cabral lattes
Orientador(a): Zilli, Jerri ?dson
Banca de defesa: Zilli, Jerri ?dson, Schultz, Nivaldo, Botelho, Gl?ria Regina, Coelho, Irene da Silva
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal Rural do Rio de Janeiro
Programa de Pós-Graduação: Programa de P?s-Gradua??o em Agronomia - Ci?ncia do Solo
Departamento: Instituto de Agronomia
País: Brasil
Palavras-chave em Português:
FBN
Taxonomia
Filogenia
Palavras-chave em Inglês:
BNF
Taxonomy
Phylogeny
Área do conhecimento CNPq:
Agronomia
Link de acesso: https://tede.ufrrj.br/jspui/handle/jspui/2234
Resumo: Centrolobium paraense Tul. popularly known as "pau-rainha", is a naturally occurring legume species from the northern state of Roraima (Brazil) to Panama. It has economic, social and environmental importance for local populations, being a nitrogen fixing species in association with bacteria of the rhizobia group. Thirteen Gram-negative, aerobic, motile with polar flagella, rods shaped bacteria previously isolated from root nodules of C. paraense grown in soils from the Roraima State were submitted to a polyphasic approach characterization. The study involved the carbon source utilization, enzymatic reaction, antibody, pH and NaCl tolerance, 16S rRNA, nodC, nifH and multi locus sequence phylogenetic analysis (MLSA with dnaK, glnII, recA, gyrB and rpoB), G+C content and Average Nucleotide Identity (ANI). Growth of strains was observed at temperature range 20- 36?C (optimal 28?C), pH ranges 5-11 (optimal 6.0-7.0) and 0.1-0.5%NaCl (optimal 0.1- 0.3%). Analysis of 16S rRNA gene placed the strains into two groups within Bradyrhizobium. Closest neighbouring species (98.8%) for group I was B. neotropicale while for group II were twelve species with more than 99% of similarity. MLSA confirmed B. neotropicale BR 10247T as the closest type strain for the group I and B. elkanii USDA 76T and B. pachyrhizi PAC 48T for group II. ANI differentiated group I from the B. neotropicale BR 10247T (79.6%), and group II from B. elkanii USDA 76T and B. pachyrhizi PAC 48T (88.1% and 87.9%, respectively). Fatty acid profiles (majority C16:0 and Summed feature 8 (18:1?6c/18:1?7c) for both groups), DNA G+C content and carbon compound utilization supported the placement of the novel strains in the genus Bradyrhizobium. Gene nodC and nifH of the new strains have in general low similarity with other Bradyrhizobium species. Both groups nodulated plants from the tribes Crotalarieae, Dalbergiae, Genisteae and Phaseoleae. Based on the result obtained, two novel species which the names Bradyrhizobium centrolobii and Bradyrhizobium macuxiense are proposed.
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spelling Zilli, Jerri ?dson927.881.579-91Zilli, Jerri ?dsonSchultz, NivaldoBotelho, Gl?ria ReginaCoelho, Irene da Silva112.148.507-31http://lattes.cnpq.br/5016121544662212Michel, Daniele Cabral2018-03-23T19:09:07Z2017-02-14MICHEL, Daniele Cabral. Caracteriza??o de estirpes de Bradyrhizobium isoladas de Centrolobium paraense. 2017. 67 f. Disserta??o (Mestrado em Agronomia - Ci?ncia do Solo). Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ, 2017.https://tede.ufrrj.br/jspui/handle/jspui/2234Centrolobium paraense Tul. popularly known as "pau-rainha", is a naturally occurring legume species from the northern state of Roraima (Brazil) to Panama. It has economic, social and environmental importance for local populations, being a nitrogen fixing species in association with bacteria of the rhizobia group. Thirteen Gram-negative, aerobic, motile with polar flagella, rods shaped bacteria previously isolated from root nodules of C. paraense grown in soils from the Roraima State were submitted to a polyphasic approach characterization. The study involved the carbon source utilization, enzymatic reaction, antibody, pH and NaCl tolerance, 16S rRNA, nodC, nifH and multi locus sequence phylogenetic analysis (MLSA with dnaK, glnII, recA, gyrB and rpoB), G+C content and Average Nucleotide Identity (ANI). Growth of strains was observed at temperature range 20- 36?C (optimal 28?C), pH ranges 5-11 (optimal 6.0-7.0) and 0.1-0.5%NaCl (optimal 0.1- 0.3%). Analysis of 16S rRNA gene placed the strains into two groups within Bradyrhizobium. Closest neighbouring species (98.8%) for group I was B. neotropicale while for group II were twelve species with more than 99% of similarity. MLSA confirmed B. neotropicale BR 10247T as the closest type strain for the group I and B. elkanii USDA 76T and B. pachyrhizi PAC 48T for group II. ANI differentiated group I from the B. neotropicale BR 10247T (79.6%), and group II from B. elkanii USDA 76T and B. pachyrhizi PAC 48T (88.1% and 87.9%, respectively). Fatty acid profiles (majority C16:0 and Summed feature 8 (18:1?6c/18:1?7c) for both groups), DNA G+C content and carbon compound utilization supported the placement of the novel strains in the genus Bradyrhizobium. Gene nodC and nifH of the new strains have in general low similarity with other Bradyrhizobium species. Both groups nodulated plants from the tribes Crotalarieae, Dalbergiae, Genisteae and Phaseoleae. Based on the result obtained, two novel species which the names Bradyrhizobium centrolobii and Bradyrhizobium macuxiense are proposed.Centrolobium paraense Tul. popularmente conhecido como ?pau-rainha?, ? uma esp?cie de leguminosa que ocorre naturalmente no norte do estado de Roraima (Brasil) ao Panam?. Tem import?ncia econ?mica, social e ambiental para popula??es locais, sendo uma esp?cie fixadora de nitrog?nio (N) em associa??o com bact?rias do grupo dos riz?bios. Treze bact?rias gram-negativas, aer?bicas, com mobilidade atrav?s de flagelos polares e em forma de bastonetes previamente isoladas de n?dulos de ra?zes de Centrolobium paraense cultivadas em solos do estado de Roraima foram submetidas a caracteriza??o com abordagem polif?sica. O estudo envolveu a utiliza??o de fontes de carbono, rea??es enzim?ticas, composi??o de ?cidos graxos das c?lulas, antibi?ticos, pH e toler?ncia a NaCl,16S rRNA, an?lise filogen?tica dos genes nodC, nifH e de sequ?ncias multilocus (MLSA com dnaK, glnII, recA, gyrB e rpoB), conte?do de G+C e identidade m?dia de nucleot?deos (ANI). O crescimento das estirpes foi observado num intervalo de temperatura 20-36?C (?tima 28?C), intervalo de pH de 5-11 (?timo 6,0-7,0) e 0,1-0,5% NaCl (?timo 0,1-0,3%). A an?lise do gene 16S rRNA posicionou as estirpes em dois grupos dentro de Bradyrhizobium. A esp?cie mais pr?xima (98,8%) para o grupo I foi B. neotropicale enquanto para o grupo II foram outras doze esp?cies com mais de 99% de similaridade. MLSA confirmou B. neotropicale BR 10247T como a estirpe tipo mais pr?xima do grupo I e B. elkanii USDA 76T e B. pachyrhizi PAC 48T do grupo II. O ANI diferenciou o grupo I da B. neotropicale BR 10247T (79,6%) e o grupo II da B. elkanii USDA 76T e B. pachyrhizi PAC 48T (88,1 e 87,9 respectivamente. O perfil de ?cidos graxos (predomin?ncia de C16:0 e caracterist?ca somada 8 (18:1?6c/18:1?7c) para ambos os grupos, ANI, o conte?do de G+C e a utiliza??o de compostos de carbono deram suporte ao posicionamento das novas estirpes no g?nero Bradyrhizobium. Os genes nodC e nifH t?m em geral baixa similaridade com outras esp?cies de Bradyrhizobium. Ambos os grupos nodularam plantas de diferentes tribos.Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2018-03-23T19:09:07Z No. of bitstreams: 1 2017 - Daniele Cabral Michel.pdf: 1915531 bytes, checksum: af036434bb0fd939d8e85a7ff4a1d43f (MD5)Made available in DSpace on 2018-03-23T19:09:07Z (GMT). No. of bitstreams: 1 2017 - Daniele Cabral Michel.pdf: 1915531 bytes, checksum: af036434bb0fd939d8e85a7ff4a1d43f (MD5) Previous issue date: 2017-02-14CAPESFAPERJapplication/pdfhttps://tede.ufrrj.br/retrieve/5951/2017%20-%20Daniele%20Cabral%20Michel.pdf.jpghttps://tede.ufrrj.br/retrieve/20798/2017%20-%20Daniele%20Cabral%20Michel.pdf.jpghttps://tede.ufrrj.br/retrieve/27137/2017%20-%20Daniele%20Cabral%20Michel.pdf.jpghttps://tede.ufrrj.br/retrieve/33484/2017%20-%20Daniele%20Cabral%20Michel.pdf.jpghttps://tede.ufrrj.br/retrieve/39906/2017%20-%20Daniele%20Cabral%20Michel.pdf.jpghttps://tede.ufrrj.br/retrieve/46258/2017%20-%20Daniele%20Cabral%20Michel.pdf.jpghttps://tede.ufrrj.br/retrieve/52656/2017%20-%20Daniele%20Cabral%20Michel.pdf.jpghttps://tede.ufrrj.br/retrieve/59106/2017%20-%20Daniele%20Cabral%20Michel.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Agronomia - Ci?ncia do SoloUFRRJBrasilInstituto de AgronomiaBARA?NA, A.C; SILVA, DA K.; PEREIRA, G.M.D; KAMINSKI, P.E.; PERIN, L.; ZILLI, J.E. 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Gene sequences useful for predicting relatedness of whole genomes in bacteria. 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dc.title.por.fl_str_mv Caracteriza??o de estirpes de Bradyrhizobium isoladas de Centrolobium paraense
dc.title.alternative.eng.fl_str_mv Caracterization of strains isolated from Centrolobium paraense
title Caracteriza??o de estirpes de Bradyrhizobium isoladas de Centrolobium paraense
spellingShingle Caracteriza??o de estirpes de Bradyrhizobium isoladas de Centrolobium paraense
Michel, Daniele Cabral
BNF
Taxonomy
Phylogeny
FBN
Taxonomia
Filogenia
Agronomia
title_short Caracteriza??o de estirpes de Bradyrhizobium isoladas de Centrolobium paraense
title_full Caracteriza??o de estirpes de Bradyrhizobium isoladas de Centrolobium paraense
title_fullStr Caracteriza??o de estirpes de Bradyrhizobium isoladas de Centrolobium paraense
title_full_unstemmed Caracteriza??o de estirpes de Bradyrhizobium isoladas de Centrolobium paraense
title_sort Caracteriza??o de estirpes de Bradyrhizobium isoladas de Centrolobium paraense
author Michel, Daniele Cabral
author_facet Michel, Daniele Cabral
author_role author
dc.contributor.advisor1.fl_str_mv Zilli, Jerri ?dson
dc.contributor.advisor1ID.fl_str_mv 927.881.579-91
dc.contributor.referee1.fl_str_mv Zilli, Jerri ?dson
dc.contributor.referee2.fl_str_mv Schultz, Nivaldo
dc.contributor.referee3.fl_str_mv Botelho, Gl?ria Regina
dc.contributor.referee4.fl_str_mv Coelho, Irene da Silva
dc.contributor.authorID.fl_str_mv 112.148.507-31
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/5016121544662212
dc.contributor.author.fl_str_mv Michel, Daniele Cabral
contributor_str_mv Zilli, Jerri ?dson
Zilli, Jerri ?dson
Schultz, Nivaldo
Botelho, Gl?ria Regina
Coelho, Irene da Silva
dc.subject.eng.fl_str_mv BNF
Taxonomy
Phylogeny
topic BNF
Taxonomy
Phylogeny
FBN
Taxonomia
Filogenia
Agronomia
dc.subject.por.fl_str_mv FBN
Taxonomia
Filogenia
dc.subject.cnpq.fl_str_mv Agronomia
description Centrolobium paraense Tul. popularly known as "pau-rainha", is a naturally occurring legume species from the northern state of Roraima (Brazil) to Panama. It has economic, social and environmental importance for local populations, being a nitrogen fixing species in association with bacteria of the rhizobia group. Thirteen Gram-negative, aerobic, motile with polar flagella, rods shaped bacteria previously isolated from root nodules of C. paraense grown in soils from the Roraima State were submitted to a polyphasic approach characterization. The study involved the carbon source utilization, enzymatic reaction, antibody, pH and NaCl tolerance, 16S rRNA, nodC, nifH and multi locus sequence phylogenetic analysis (MLSA with dnaK, glnII, recA, gyrB and rpoB), G+C content and Average Nucleotide Identity (ANI). Growth of strains was observed at temperature range 20- 36?C (optimal 28?C), pH ranges 5-11 (optimal 6.0-7.0) and 0.1-0.5%NaCl (optimal 0.1- 0.3%). Analysis of 16S rRNA gene placed the strains into two groups within Bradyrhizobium. Closest neighbouring species (98.8%) for group I was B. neotropicale while for group II were twelve species with more than 99% of similarity. MLSA confirmed B. neotropicale BR 10247T as the closest type strain for the group I and B. elkanii USDA 76T and B. pachyrhizi PAC 48T for group II. ANI differentiated group I from the B. neotropicale BR 10247T (79.6%), and group II from B. elkanii USDA 76T and B. pachyrhizi PAC 48T (88.1% and 87.9%, respectively). Fatty acid profiles (majority C16:0 and Summed feature 8 (18:1?6c/18:1?7c) for both groups), DNA G+C content and carbon compound utilization supported the placement of the novel strains in the genus Bradyrhizobium. Gene nodC and nifH of the new strains have in general low similarity with other Bradyrhizobium species. Both groups nodulated plants from the tribes Crotalarieae, Dalbergiae, Genisteae and Phaseoleae. Based on the result obtained, two novel species which the names Bradyrhizobium centrolobii and Bradyrhizobium macuxiense are proposed.
publishDate 2017
dc.date.issued.fl_str_mv 2017-02-14
dc.date.accessioned.fl_str_mv 2018-03-23T19:09:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv MICHEL, Daniele Cabral. Caracteriza??o de estirpes de Bradyrhizobium isoladas de Centrolobium paraense. 2017. 67 f. Disserta??o (Mestrado em Agronomia - Ci?ncia do Solo). Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ, 2017.
dc.identifier.uri.fl_str_mv https://tede.ufrrj.br/jspui/handle/jspui/2234
identifier_str_mv MICHEL, Daniele Cabral. Caracteriza??o de estirpes de Bradyrhizobium isoladas de Centrolobium paraense. 2017. 67 f. Disserta??o (Mestrado em Agronomia - Ci?ncia do Solo). Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ, 2017.
url https://tede.ufrrj.br/jspui/handle/jspui/2234
dc.language.iso.fl_str_mv por
language por
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