Detec??o e caracteriza??o molecular do Poxv?rus Avi?rio em Culex sp. coletados no peridomic?lio de ?reas rurais e periurbanas no munic?pio de Serop?dica, Estado do Rio de Janeiro

Detalhes bibliográficos
Ano de defesa: 2021
Autor(a) principal: Meer, Carolina Soares van der lattes
Orientador(a): Santos, Huarrisson Azevedo lattes
Banca de defesa: Santos, Huarrisson Azevedo lattes, Souza, Paulo C?sar Augusto de lattes, Guedes Junior, Daniel da Silva lattes
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal Rural do Rio de Janeiro
Programa de Pós-Graduação: Programa de P?s-Gradua??o em Ci?ncias Veterin?rias
Departamento: Instituto de Veterin?ria
País: Brasil
Palavras-chave em Português:
Bouba Avi?ria
Culex
Filogenia
Detec??o Molecular
Poxv?rus Avi?rio
Palavras-chave em Inglês:
Avian Pox
Culex
Phylogeny
Molecular Detection
Avipoxvirus
Área do conhecimento CNPq:
Medicina Veterin?ria
Link de acesso: https://tede.ufrrj.br/jspui/handle/jspui/6433
Resumo: Avian Pox is a highly contagious viral disease whose largest geographical range of cases covers tropical and subtropical regions, affecting both domestic and wild birds. Experimental studies show that the main form of transmission occurs during blood feeding by vectors of the genus Culex, previously fed with the blood of infected birds. Therefore, the aim of this study was to establish the frequency of Culex sp. with Avipoxvirus DNA through PCR assay, in the municipality of Serop?dica, Rio de Janeiro and then perform a phylogenetic analysis based on the p4b gene from positive samples. In this study, 2839 mosquitoes belonging to Anopheles, Aedes and Culex genera were collected, with the Culex sp. genus being the most abundant, comprising 96.23% (2732/2839) of the total amount, where the Avipoxvirus detection was conducted on 300 fed females from the neighborhoods: S?o Miguel, Val?o das Lou?as and Campo Lindo. Of the collected specimens, a total of 13.66% (41/300) of the samples of females tested were positive in the detection reaction, where 13.63% of the positives (21/154) were collected at Campo Lindo neighborhood, 16.67% (16/96) at S?o Miguel and 12% (6/50) at Val?o das Lou?as. There was no significant difference in the frequency of Avipoxvirus in Culex sp. among the neighborhoods analyzed (p>0.05).The phylogenetic analysis was performed comparing 347 sequences available in GenBank, in order to group the subclades A1 to A7, B1 to B3 and C, and, for the first time in Brazil, it was possible to report the presence of subclade A1 in samples of Culex sp. with Avipoxvirus DNA. The evolutionary distance of the p4b gene of the sequenced samples, compared between the evaluated areas, was able to demonstrate larger genetic divergence in rural areas (0.0061?0.0021) when compared to periurban areas (0.0038?0.0016) in this study. This work enabled the improvement of an effective PCR technique for the detection of Avipoxvirus directly in Culex sp. DNA, in addition to complementing data on vector biology and improving the monitoring and control techniques of Avian Pox in the State of Rio de Janeiro
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spelling Santos, Huarrisson Azevedo983.833.295-04https://orcid.org/0000-0002-8218-3626http://lattes.cnpq.br/3751609492049306Massard, Carlos Luiz257.781.297-34https://orcid.org/0000-0002-8465-3038http://lattes.cnpq.br/7743112049924654Santos, Huarrisson Azevedo983.833.295-04https://orcid.org/0000-0002-8218-3626http://lattes.cnpq.br/3751609492049306Souza, Paulo C?sar Augusto dehttp://lattes.cnpq.br/2610207757764925Guedes Junior, Daniel da Silva072.876.757-06http://lattes.cnpq.br/1514734234282622148.903797-70https://orcid.org/0000-0002-4930-4537http://lattes.cnpq.br/0239103577839599Meer, Carolina Soares van der2023-03-20T14:13:05Z2021-07-08MEER, Carolina Soares van der. Detec??o e caracteriza??o molecular do Poxv?rus Avi?rio em Culex sp. coletados no peridomic?lio de ?reas rurais e periurbanas no munic?pio de Serop?dica, Estado do Rio de Janeiro. 2021.75 f. Disserta??o (Mestrado em Ci?ncias Veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2021.https://tede.ufrrj.br/jspui/handle/jspui/6433Avian Pox is a highly contagious viral disease whose largest geographical range of cases covers tropical and subtropical regions, affecting both domestic and wild birds. Experimental studies show that the main form of transmission occurs during blood feeding by vectors of the genus Culex, previously fed with the blood of infected birds. Therefore, the aim of this study was to establish the frequency of Culex sp. with Avipoxvirus DNA through PCR assay, in the municipality of Serop?dica, Rio de Janeiro and then perform a phylogenetic analysis based on the p4b gene from positive samples. In this study, 2839 mosquitoes belonging to Anopheles, Aedes and Culex genera were collected, with the Culex sp. genus being the most abundant, comprising 96.23% (2732/2839) of the total amount, where the Avipoxvirus detection was conducted on 300 fed females from the neighborhoods: S?o Miguel, Val?o das Lou?as and Campo Lindo. Of the collected specimens, a total of 13.66% (41/300) of the samples of females tested were positive in the detection reaction, where 13.63% of the positives (21/154) were collected at Campo Lindo neighborhood, 16.67% (16/96) at S?o Miguel and 12% (6/50) at Val?o das Lou?as. There was no significant difference in the frequency of Avipoxvirus in Culex sp. among the neighborhoods analyzed (p>0.05).The phylogenetic analysis was performed comparing 347 sequences available in GenBank, in order to group the subclades A1 to A7, B1 to B3 and C, and, for the first time in Brazil, it was possible to report the presence of subclade A1 in samples of Culex sp. with Avipoxvirus DNA. The evolutionary distance of the p4b gene of the sequenced samples, compared between the evaluated areas, was able to demonstrate larger genetic divergence in rural areas (0.0061?0.0021) when compared to periurban areas (0.0038?0.0016) in this study. This work enabled the improvement of an effective PCR technique for the detection of Avipoxvirus directly in Culex sp. DNA, in addition to complementing data on vector biology and improving the monitoring and control techniques of Avian Pox in the State of Rio de JaneiroA Bouba Avi?ria ? uma enfermidade viral altamente contagiosa cuja maior abrang?ncia geogr?fica de casos contempla regi?es tropicais e subtropicais, afetando aves dom?sticas e selvagens. Estudos experimentais apontam que a principal forma de transmiss?o se d? atrav?s do repasto sangu?neo com dispers?o viral realizada por mosquitos vetores do g?nero Culex, alimentados com sangue de aves infectadas. Desta forma, o objetivo desse estudo foi estabelecer a frequ?ncia de Culex sp. com material gen?tico de Avipoxv?rus atrav?s da t?cnica de PCR no peridomic?lio de ?reas rurais e periurbanas do munic?pio de Serop?dica, no Rio de Janeiro e realizar an?lise filogen?tica com base no gene p4b a partir de amostras positivas. Foram coletados 2839 mosquitos dos g?neros Anopheles, Aedes e Culex, sendo o g?nero Culex o mais abundante, compreendendo 96,23% (2732/2839) do montante total, onde a pesquisa de Avipoxv?rus foi realizada em 300 f?meas alimentadas oriundas dos bairros de S?o Miguel, Val?o das Lou?as e Campo Lindo. Dos esp?cimes coletados, um total de 13,66% (41/300) das amostras de f?meas testadas apresentaram-se positivas na rea??o de detec??o, onde 13,63% das positivas (21/154) foram coletadas no bairro de Campo Lindo, 16,67% (16/96) em S?o Miguel e 12% (6/50) em Val?o das Lou?as. N?o houve diferen?a significativa na frequ?ncia de Avipoxv?rus em Culex sp. entre os bairros analisados (p>0,05). A an?lise filogen?tica foi realizada comparando 347 sequ?ncias dispon?veis no GenBank, de forma a agrupar os subclados A1 a A7, B1 a B3 e C. Pela primeira vez no Brasil, foi poss?vel reportar a presen?a do subclado A1 em amostras oriundas de Culex sp. contendo DNA de Avipoxv?rus. A dist?ncia evolutiva do gene p4b das amostras sequenciadas, comparada entre as ?reas avaliadas, foi capaz de demonstrar maior diverg?ncia gen?tica em ?reas rurais (0,0061?0,0021) quando comparada a ?reas periurbanas (0,0038?0,0016) nesse estudo. Esse trabalho possibilitou a otimiza??o de uma t?cnica de PCR eficaz para detec??o de Avipoxv?rus diretamente do DNA extra?do de Culex sp., al?m da complementa??o de dados referentes ? biologia do vetor e melhoria das t?cnicas de monitoramento e de controle da Bouba Avi?ria no Estado do Rio de JaneiroSubmitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2023-03-20T14:13:05Z No. of bitstreams: 1 2021 - Carolina Soares van der Meer.pdf: 2844868 bytes, checksum: a69c5007281b529a39eade360a4a255d (MD5)Made available in DSpace on 2023-03-20T14:13:05Z (GMT). No. of bitstreams: 1 2021 - Carolina Soares van der Meer.pdf: 2844868 bytes, checksum: a69c5007281b529a39eade360a4a255d (MD5) Previous issue date: 2021-07-08CAPES - Coordena??o de Aperfei?oamento de Pessoal de N?vel SuperiorCNPQ - Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gicoLASAVE - Laborat?rio de Sanidade Av?colaapplication/pdfhttps://tede.ufrrj.br/retrieve/72605/2021%20-%20Carolina%20Soares%20van%20der%20Meer.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Ci?ncias Veterin?riasUFRRJBrasilInstituto de Veterin?riaABPA. Associa??o Brasileira de Prote?na Animal. Relat?rio Anual 2020. Acesso em 10/03/21. Dispon?vel em: https://abpa-br.org/wp-content/uploads/2020/05/abpa_relatorio_anual_2020_portugues_web.pdf. 2020. ALMEIDA A. P. G. Os mosquitos (Diptera, Culicidae) e sua Import?ncia M?dica em Portugal. Desafios para o S?culo XXI. Acta Med Port, 24: 961-974, 2011. AFONSO, C. L.; TULMAN, E. R.; LU, Z.; ZSAK, L.; KUTISH, G. F.; ROCK, D. L. 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dc.title.por.fl_str_mv Detec??o e caracteriza??o molecular do Poxv?rus Avi?rio em Culex sp. coletados no peridomic?lio de ?reas rurais e periurbanas no munic?pio de Serop?dica, Estado do Rio de Janeiro
dc.title.alternative.eng.fl_str_mv Detection and molecular characterization of Avipoxvirus in Culex sp. collected in the peridomicile of rural and periurban areas in the municipality of Serop?dica, Rio de Janeiro.
title Detec??o e caracteriza??o molecular do Poxv?rus Avi?rio em Culex sp. coletados no peridomic?lio de ?reas rurais e periurbanas no munic?pio de Serop?dica, Estado do Rio de Janeiro
spellingShingle Detec??o e caracteriza??o molecular do Poxv?rus Avi?rio em Culex sp. coletados no peridomic?lio de ?reas rurais e periurbanas no munic?pio de Serop?dica, Estado do Rio de Janeiro
Meer, Carolina Soares van der
Bouba Avi?ria
Culex
Filogenia
Detec??o Molecular
Poxv?rus Avi?rio
Avian Pox
Culex
Phylogeny
Molecular Detection
Avipoxvirus
Medicina Veterin?ria
title_short Detec??o e caracteriza??o molecular do Poxv?rus Avi?rio em Culex sp. coletados no peridomic?lio de ?reas rurais e periurbanas no munic?pio de Serop?dica, Estado do Rio de Janeiro
title_full Detec??o e caracteriza??o molecular do Poxv?rus Avi?rio em Culex sp. coletados no peridomic?lio de ?reas rurais e periurbanas no munic?pio de Serop?dica, Estado do Rio de Janeiro
title_fullStr Detec??o e caracteriza??o molecular do Poxv?rus Avi?rio em Culex sp. coletados no peridomic?lio de ?reas rurais e periurbanas no munic?pio de Serop?dica, Estado do Rio de Janeiro
title_full_unstemmed Detec??o e caracteriza??o molecular do Poxv?rus Avi?rio em Culex sp. coletados no peridomic?lio de ?reas rurais e periurbanas no munic?pio de Serop?dica, Estado do Rio de Janeiro
title_sort Detec??o e caracteriza??o molecular do Poxv?rus Avi?rio em Culex sp. coletados no peridomic?lio de ?reas rurais e periurbanas no munic?pio de Serop?dica, Estado do Rio de Janeiro
author Meer, Carolina Soares van der
author_facet Meer, Carolina Soares van der
author_role author
dc.contributor.advisor1.fl_str_mv Santos, Huarrisson Azevedo
dc.contributor.advisor1ID.fl_str_mv 983.833.295-04
https://orcid.org/0000-0002-8218-3626
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/3751609492049306
dc.contributor.advisor-co1.fl_str_mv Massard, Carlos Luiz
dc.contributor.advisor-co1ID.fl_str_mv 257.781.297-34
https://orcid.org/0000-0002-8465-3038
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/7743112049924654
dc.contributor.referee1.fl_str_mv Santos, Huarrisson Azevedo
dc.contributor.referee1ID.fl_str_mv 983.833.295-04
https://orcid.org/0000-0002-8218-3626
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/3751609492049306
dc.contributor.referee2.fl_str_mv Souza, Paulo C?sar Augusto de
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/2610207757764925
dc.contributor.referee3.fl_str_mv Guedes Junior, Daniel da Silva
dc.contributor.referee3ID.fl_str_mv 072.876.757-06
dc.contributor.referee3Lattes.fl_str_mv http://lattes.cnpq.br/1514734234282622
dc.contributor.authorID.fl_str_mv 148.903797-70
https://orcid.org/0000-0002-4930-4537
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/0239103577839599
dc.contributor.author.fl_str_mv Meer, Carolina Soares van der
contributor_str_mv Santos, Huarrisson Azevedo
Massard, Carlos Luiz
Santos, Huarrisson Azevedo
Souza, Paulo C?sar Augusto de
Guedes Junior, Daniel da Silva
dc.subject.por.fl_str_mv Bouba Avi?ria
Culex
Filogenia
Detec??o Molecular
Poxv?rus Avi?rio
topic Bouba Avi?ria
Culex
Filogenia
Detec??o Molecular
Poxv?rus Avi?rio
Avian Pox
Culex
Phylogeny
Molecular Detection
Avipoxvirus
Medicina Veterin?ria
dc.subject.eng.fl_str_mv Avian Pox
Culex
Phylogeny
Molecular Detection
Avipoxvirus
dc.subject.cnpq.fl_str_mv Medicina Veterin?ria
description Avian Pox is a highly contagious viral disease whose largest geographical range of cases covers tropical and subtropical regions, affecting both domestic and wild birds. Experimental studies show that the main form of transmission occurs during blood feeding by vectors of the genus Culex, previously fed with the blood of infected birds. Therefore, the aim of this study was to establish the frequency of Culex sp. with Avipoxvirus DNA through PCR assay, in the municipality of Serop?dica, Rio de Janeiro and then perform a phylogenetic analysis based on the p4b gene from positive samples. In this study, 2839 mosquitoes belonging to Anopheles, Aedes and Culex genera were collected, with the Culex sp. genus being the most abundant, comprising 96.23% (2732/2839) of the total amount, where the Avipoxvirus detection was conducted on 300 fed females from the neighborhoods: S?o Miguel, Val?o das Lou?as and Campo Lindo. Of the collected specimens, a total of 13.66% (41/300) of the samples of females tested were positive in the detection reaction, where 13.63% of the positives (21/154) were collected at Campo Lindo neighborhood, 16.67% (16/96) at S?o Miguel and 12% (6/50) at Val?o das Lou?as. There was no significant difference in the frequency of Avipoxvirus in Culex sp. among the neighborhoods analyzed (p>0.05).The phylogenetic analysis was performed comparing 347 sequences available in GenBank, in order to group the subclades A1 to A7, B1 to B3 and C, and, for the first time in Brazil, it was possible to report the presence of subclade A1 in samples of Culex sp. with Avipoxvirus DNA. The evolutionary distance of the p4b gene of the sequenced samples, compared between the evaluated areas, was able to demonstrate larger genetic divergence in rural areas (0.0061?0.0021) when compared to periurban areas (0.0038?0.0016) in this study. This work enabled the improvement of an effective PCR technique for the detection of Avipoxvirus directly in Culex sp. DNA, in addition to complementing data on vector biology and improving the monitoring and control techniques of Avian Pox in the State of Rio de Janeiro
publishDate 2021
dc.date.issued.fl_str_mv 2021-07-08
dc.date.accessioned.fl_str_mv 2023-03-20T14:13:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv MEER, Carolina Soares van der. Detec??o e caracteriza??o molecular do Poxv?rus Avi?rio em Culex sp. coletados no peridomic?lio de ?reas rurais e periurbanas no munic?pio de Serop?dica, Estado do Rio de Janeiro. 2021.75 f. Disserta??o (Mestrado em Ci?ncias Veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2021.
dc.identifier.uri.fl_str_mv https://tede.ufrrj.br/jspui/handle/jspui/6433
identifier_str_mv MEER, Carolina Soares van der. Detec??o e caracteriza??o molecular do Poxv?rus Avi?rio em Culex sp. coletados no peridomic?lio de ?reas rurais e periurbanas no munic?pio de Serop?dica, Estado do Rio de Janeiro. 2021.75 f. Disserta??o (Mestrado em Ci?ncias Veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2021.
url https://tede.ufrrj.br/jspui/handle/jspui/6433
dc.language.iso.fl_str_mv por
language por
dc.relation.references.por.fl_str_mv ABPA. Associa??o Brasileira de Prote?na Animal. Relat?rio Anual 2020. Acesso em 10/03/21. Dispon?vel em: https://abpa-br.org/wp-content/uploads/2020/05/abpa_relatorio_anual_2020_portugues_web.pdf. 2020. ALMEIDA A. P. G. Os mosquitos (Diptera, Culicidae) e sua Import?ncia M?dica em Portugal. Desafios para o S?culo XXI. Acta Med Port, 24: 961-974, 2011. AFONSO, C. L.; TULMAN, E. R.; LU, Z.; ZSAK, L.; KUTISH, G. F.; ROCK, D. L. The genome of fowl poxvirus. J Virol, 74 (8): 3815-3831. 2000. AKEY, B. L., NAYAR, J. K.; FORRESTER, D. J. Avian pox in Florida wild turkeys: Culex nigripalpus and Wyeomyia vanduzeei as experimental vectors. J. Wildl. Dis. 17(4):597-599. 1981. ALDERS, R. G.; DUMAS, S. E.; RUKAMBILE, E.; MAGOKE, G.; MAULAGA, W.; JONG, J.; COSTA, R. Family poultry: Multiple roles, systems, challenges, and options for sustainable contributions to household nutrition security through a planetary health lens. 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