Aspectos cl?nico patol?gicos e laboratoriais do envenenamento experimental por Bothrops alternatus em bovinos
Ano de defesa: | 2008 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal Rural do Rio de Janeiro
|
Programa de Pós-Graduação: |
Programa de P?s-Gradua??o em Ci?ncias Veterin?rias
|
Departamento: |
Instituto de Veterin?ria
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | https://tede.ufrrj.br/jspui/handle/tede/761 |
Resumo: | The aim of this study was to determine the clinical-pathological alterations and laboratory findings in bovines inoculated with Bothrops alternatus venom, with the intention of providing information for the establishment of diagnosis and differential diagnosis procedures, as well as to elucidate some obscurities observed in the pertinent literature. The lyophilized venom was diluted in 1 ml of physiologic solution. It was administered to 5 bovines by the subcutaneous route at doses of 0.0625, 0.125 and 0.25 mg/kg body weight, and to 2 other bovines by the intramuscular route at doses of 0.25 e 0.45 mg/kg. Six bovines died and the only animal that survived, who had subcutaneously received the venom at a dose of 0.0625 mg/kg, recovered. The first clinical signs were observed from 25 minutes to 5 hours and 30 minutes after the inoculation. The clinical evolution time varied from 7 hours and 18 minutes to 92 hours. Regardless of the dose, the clinical picture was characterized by swelling (hemorrhage/hematoma) at the site of inoculation, increase in bleeding time and capillary refill time, paleness of mucous membranes and apathy. The laboratory exams revealed progressive normocytic normochromic anemia, thrombocytopenia, reduction in fibrinogen and total plasma proteins, decreased hematocrit and hemoglobin, and slight increase in creatine kinase and lactate dehydrogenase. When subjected to necropsy, the animals that received the venom through the subcutaneous route showed large hematomas and hemorrhagic areas in the subcutaneous tissue, extending from the site of inoculation. The animals in which inoculation was performed intramuscularly exhibited, in addition, intramuscular hemorrhage. The left endocardium showed extensive hemorrhagic lesions, and petechiae were found on the serosae of rumen, omasum, abomasum and gall bladder. The colon, rectum and perirenal areas were surrounded by clotted blood in three animals. Besides hemorrhage, the histological examination revealed hemorrhage and coagulative muscle necrosis in the vicinities of the inoculation site in the animals that received the venom by the intramuscular route. These lesions were mild in the muscles close to the site of inoculation in the animals inoculated by the subcutaneous route. The swelling on the site of inoculation and its surroundings was a consequence of blood accumulation and not merely edema. Myoglobinuria and gross or microscopic lesions were not observed in the kidneys. This study indicates that B. alternatus can lead adult bovines to death if they release all their venom during a bite. Conversely, since venomous ophidians can control the amount of poison they inoculate and they probably do not identify bovines as potential preys, it is likely that the number of accidents involving this species is small, which is in agreement with the findings of the majority of the centers for anatomical-pathological diagnosis in the country. |
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Peixoto, Paulo Fernando de Vargas270.761.210-34http://lattes.cnpq.br/3002512195318611Tokarnia, Carlos Maria Ant?nio Hubinger239.959.637-49http://lattes.cnpq.br/7809700501564538076.977.047-90http://lattes.cnpq.br/3830458171009218Caldas, Saulo Andrade2016-04-28T20:15:30Z2008-07-162008-02-20CALDAS, Saulo Andrade. Aspectos cl?nico patol?gicos e laboratoriais do envenenamento experimental por Bothrops alternatus em bovinos. 2008. 111 f. Disserta??o (Mestrado em Ci?ncias Veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2008.https://tede.ufrrj.br/jspui/handle/tede/761The aim of this study was to determine the clinical-pathological alterations and laboratory findings in bovines inoculated with Bothrops alternatus venom, with the intention of providing information for the establishment of diagnosis and differential diagnosis procedures, as well as to elucidate some obscurities observed in the pertinent literature. The lyophilized venom was diluted in 1 ml of physiologic solution. It was administered to 5 bovines by the subcutaneous route at doses of 0.0625, 0.125 and 0.25 mg/kg body weight, and to 2 other bovines by the intramuscular route at doses of 0.25 e 0.45 mg/kg. Six bovines died and the only animal that survived, who had subcutaneously received the venom at a dose of 0.0625 mg/kg, recovered. The first clinical signs were observed from 25 minutes to 5 hours and 30 minutes after the inoculation. The clinical evolution time varied from 7 hours and 18 minutes to 92 hours. Regardless of the dose, the clinical picture was characterized by swelling (hemorrhage/hematoma) at the site of inoculation, increase in bleeding time and capillary refill time, paleness of mucous membranes and apathy. The laboratory exams revealed progressive normocytic normochromic anemia, thrombocytopenia, reduction in fibrinogen and total plasma proteins, decreased hematocrit and hemoglobin, and slight increase in creatine kinase and lactate dehydrogenase. When subjected to necropsy, the animals that received the venom through the subcutaneous route showed large hematomas and hemorrhagic areas in the subcutaneous tissue, extending from the site of inoculation. The animals in which inoculation was performed intramuscularly exhibited, in addition, intramuscular hemorrhage. The left endocardium showed extensive hemorrhagic lesions, and petechiae were found on the serosae of rumen, omasum, abomasum and gall bladder. The colon, rectum and perirenal areas were surrounded by clotted blood in three animals. Besides hemorrhage, the histological examination revealed hemorrhage and coagulative muscle necrosis in the vicinities of the inoculation site in the animals that received the venom by the intramuscular route. These lesions were mild in the muscles close to the site of inoculation in the animals inoculated by the subcutaneous route. The swelling on the site of inoculation and its surroundings was a consequence of blood accumulation and not merely edema. Myoglobinuria and gross or microscopic lesions were not observed in the kidneys. This study indicates that B. alternatus can lead adult bovines to death if they release all their venom during a bite. Conversely, since venomous ophidians can control the amount of poison they inoculate and they probably do not identify bovines as potential preys, it is likely that the number of accidents involving this species is small, which is in agreement with the findings of the majority of the centers for anatomical-pathological diagnosis in the country.Esse estudo teve como objetivo determinar as altera??es cl?nico-patol?gicas e os achados laboratoriais em bovinos inoculados com a pe?onha de Bothrops alternatus, no intuito de fornecer subs?dios para o estabelecimento do diagn?stico e do diagn?stico diferencial, bem como esclarecer pontos obscuros da literatura pertinente. O veneno liofilizado foi dilu?do em 1 ml de solu??o fisiol?gica e administrado a cinco bovinos, por via subcut?nea, nas doses de 0,0625, 0,125 e 0,25 mg/kg e a dois outros, por via intramuscular, nas doses de 0,25 e 0,45 mg/kg. Seis bovinos foram a ?bito e um que recebeu a dose de 0,0625 mg/kg, por via subcut?nea, recuperou-se. Os sinais cl?nicos tiveram in?cio entre 25 minutos e 5 horas e 30 minutos ap?s a inocula??o. O per?odo de evolu??o variou de 7 horas e 18 minutos a 66 horas e 12 minutos. Um animal recuperou-se ap?s 92 horas. O quadro cl?nico, independentemente das doses, caracterizou-se por aumento de volume (hemorragia/hematoma) no local da inocula??o, tempo de sangramento aumentado, mucosas hipocoradas e apatia. O exame laboratorial revelou progressiva anemia normoc?tica normocr?mica, trombocitopenia, redu??o de fibrinog?nio e prote?nas plasm?ticas totais, hemat?crito e hemoglobina diminu?dos, al?m de leve aumento dos n?veis de creatinaquinase e desidrogenase l?tica. ? necropsia, havia, a partir do local da inocula??o, extensos hematomas e ?reas de hemorragia no tecido celular subcut?neo dos animais que receberam o veneno por via subcut?nea; nos animais inoculados por via intramuscular, adicionalmente, havia hemorragia intramuscular. O endoc?rdio esquerdo apresentava extensas hemorragias e verificaram-se pet?quias na serosa do r?men e do omaso e na mucosa do abomaso e da ves?cula biliar. Em cinco animais, o c?lon, reto e regi?o perirrenal estavam envoltos por co?gulos de sangue. Ao exame histol?gico observouse, al?m do quadro hemorrag?paro, necrose muscular coagulativa, acompanhada de hemorragia, no entorno do local da inocula??o nos animais que receberam o veneno por via intramuscular; essa les?o era discreta nos m?sculos pr?ximos ao local de inocula??o subcut?nea. Nos bovinos deste estudo, o aumento de volume observado no local de inocula??o e adjac?ncias era constitu?do por sangue e n?o edema. N?o foram observadas mioglobin?ria, nem les?es macro ou microsc?picas significativas nos rins. Este estudo indica que exemplares de B. alternatus, caso inoculem todo seu veneno, poderiam levar bovinos adultos ? morte. Por outro lado, pelo fato de of?dios serem capazes de regular a quantidade de veneno inoculada e, possivelmente, n?o considerarem bovinos como presa potencial, ? prov?vel que o n?mero de acidentes nessa esp?cie seja pequeno, o que est? de acordo com o observado na maioria dos centros diagn?stico an?tomo-patol?gico no pa?s.Made available in DSpace on 2016-04-28T20:15:30Z (GMT). No. of bitstreams: 1 2008 - Saulo Andrade Caldas.pdf: 2660226 bytes, checksum: 9633b16ad905372a6086d20f9fab7136 (MD5) Previous issue date: 2008-02-20FAPERJ - Funda??o Carlos Chagas Filho de Amparo ? Pesquisa do Estado do Rio de Janeiroapplication/pdfhttps://tede.ufrrj.br/retrieve/59739/2008%20-%20Saulo%20Andrade%20Caldas.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Ci?ncias Veterin?riasUFRRJBrasilInstituto de Veterin?riaBothrops alternatusbovinospatologiacattlepathologyMedicina Veterin?riaAspectos cl?nico patol?gicos e laboratoriais do envenenamento experimental por Bothrops alternatus em bovinosClinic-pathological and laboratory aspects of experimental poisoning by Bothrops alternatus in bovinesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRRJinstname:Universidade Federal Rural do Rio de Janeiro (UFRRJ)instacron:UFRRJTHUMBNAIL2008 - Saulo Andrade Caldas.pdf.jpg2008 - Saulo Andrade Caldas.pdf.jpgimage/jpeg1943http://localhost:8080/tede/bitstream/tede/761/3/2008+-+Saulo+Andrade+Caldas.pdf.jpgcc73c4c239a4c332d642ba1e7c7a9fb2MD53TEXT2008 - Saulo Andrade Caldas.pdf.txt2008 - Saulo Andrade Caldas.pdf.txttext/plain165874http://localhost:8080/tede/bitstream/tede/761/2/2008+-+Saulo+Andrade+Caldas.pdf.txt33e039849848d19c975ed11ca6f61d5dMD52ORIGINAL2008 - Saulo Andrade Caldas.pdf2008 - Saulo Andrade Caldas.pdfapplication/pdf2660450http://localhost:8080/tede/bitstream/tede/761/1/2008+-+Saulo+Andrade+Caldas.pdf7ed18c16e3d1fe811bbf4be9b9be50c7MD51tede/7612022-06-01 17:20:57.906oai:localhost:tede/761Biblioteca Digital de Teses e Dissertaçõeshttps://tede.ufrrj.br/PUBhttps://tede.ufrrj.br/oai/requestbibliot@ufrrj.br||bibliot@ufrrj.bropendoar:2022-06-01T20:20:57Biblioteca Digital de Teses e Dissertações da UFRRJ - Universidade Federal Rural do Rio de Janeiro (UFRRJ)false |
dc.title.por.fl_str_mv |
Aspectos cl?nico patol?gicos e laboratoriais do envenenamento experimental por Bothrops alternatus em bovinos |
dc.title.alternative.eng.fl_str_mv |
Clinic-pathological and laboratory aspects of experimental poisoning by Bothrops alternatus in bovines |
title |
Aspectos cl?nico patol?gicos e laboratoriais do envenenamento experimental por Bothrops alternatus em bovinos |
spellingShingle |
Aspectos cl?nico patol?gicos e laboratoriais do envenenamento experimental por Bothrops alternatus em bovinos Caldas, Saulo Andrade Bothrops alternatus bovinos patologia cattle pathology Medicina Veterin?ria |
title_short |
Aspectos cl?nico patol?gicos e laboratoriais do envenenamento experimental por Bothrops alternatus em bovinos |
title_full |
Aspectos cl?nico patol?gicos e laboratoriais do envenenamento experimental por Bothrops alternatus em bovinos |
title_fullStr |
Aspectos cl?nico patol?gicos e laboratoriais do envenenamento experimental por Bothrops alternatus em bovinos |
title_full_unstemmed |
Aspectos cl?nico patol?gicos e laboratoriais do envenenamento experimental por Bothrops alternatus em bovinos |
title_sort |
Aspectos cl?nico patol?gicos e laboratoriais do envenenamento experimental por Bothrops alternatus em bovinos |
author |
Caldas, Saulo Andrade |
author_facet |
Caldas, Saulo Andrade |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Peixoto, Paulo Fernando de Vargas |
dc.contributor.advisor1ID.fl_str_mv |
270.761.210-34 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3002512195318611 |
dc.contributor.advisor-co1.fl_str_mv |
Tokarnia, Carlos Maria Ant?nio Hubinger |
dc.contributor.advisor-co1ID.fl_str_mv |
239.959.637-49 |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/7809700501564538 |
dc.contributor.authorID.fl_str_mv |
076.977.047-90 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3830458171009218 |
dc.contributor.author.fl_str_mv |
Caldas, Saulo Andrade |
contributor_str_mv |
Peixoto, Paulo Fernando de Vargas Tokarnia, Carlos Maria Ant?nio Hubinger |
dc.subject.por.fl_str_mv |
Bothrops alternatus bovinos patologia |
topic |
Bothrops alternatus bovinos patologia cattle pathology Medicina Veterin?ria |
dc.subject.eng.fl_str_mv |
cattle pathology |
dc.subject.cnpq.fl_str_mv |
Medicina Veterin?ria |
description |
The aim of this study was to determine the clinical-pathological alterations and laboratory findings in bovines inoculated with Bothrops alternatus venom, with the intention of providing information for the establishment of diagnosis and differential diagnosis procedures, as well as to elucidate some obscurities observed in the pertinent literature. The lyophilized venom was diluted in 1 ml of physiologic solution. It was administered to 5 bovines by the subcutaneous route at doses of 0.0625, 0.125 and 0.25 mg/kg body weight, and to 2 other bovines by the intramuscular route at doses of 0.25 e 0.45 mg/kg. Six bovines died and the only animal that survived, who had subcutaneously received the venom at a dose of 0.0625 mg/kg, recovered. The first clinical signs were observed from 25 minutes to 5 hours and 30 minutes after the inoculation. The clinical evolution time varied from 7 hours and 18 minutes to 92 hours. Regardless of the dose, the clinical picture was characterized by swelling (hemorrhage/hematoma) at the site of inoculation, increase in bleeding time and capillary refill time, paleness of mucous membranes and apathy. The laboratory exams revealed progressive normocytic normochromic anemia, thrombocytopenia, reduction in fibrinogen and total plasma proteins, decreased hematocrit and hemoglobin, and slight increase in creatine kinase and lactate dehydrogenase. When subjected to necropsy, the animals that received the venom through the subcutaneous route showed large hematomas and hemorrhagic areas in the subcutaneous tissue, extending from the site of inoculation. The animals in which inoculation was performed intramuscularly exhibited, in addition, intramuscular hemorrhage. The left endocardium showed extensive hemorrhagic lesions, and petechiae were found on the serosae of rumen, omasum, abomasum and gall bladder. The colon, rectum and perirenal areas were surrounded by clotted blood in three animals. Besides hemorrhage, the histological examination revealed hemorrhage and coagulative muscle necrosis in the vicinities of the inoculation site in the animals that received the venom by the intramuscular route. These lesions were mild in the muscles close to the site of inoculation in the animals inoculated by the subcutaneous route. The swelling on the site of inoculation and its surroundings was a consequence of blood accumulation and not merely edema. Myoglobinuria and gross or microscopic lesions were not observed in the kidneys. This study indicates that B. alternatus can lead adult bovines to death if they release all their venom during a bite. Conversely, since venomous ophidians can control the amount of poison they inoculate and they probably do not identify bovines as potential preys, it is likely that the number of accidents involving this species is small, which is in agreement with the findings of the majority of the centers for anatomical-pathological diagnosis in the country. |
publishDate |
2008 |
dc.date.available.fl_str_mv |
2008-07-16 |
dc.date.issued.fl_str_mv |
2008-02-20 |
dc.date.accessioned.fl_str_mv |
2016-04-28T20:15:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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dc.identifier.citation.fl_str_mv |
CALDAS, Saulo Andrade. Aspectos cl?nico patol?gicos e laboratoriais do envenenamento experimental por Bothrops alternatus em bovinos. 2008. 111 f. Disserta??o (Mestrado em Ci?ncias Veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2008. |
dc.identifier.uri.fl_str_mv |
https://tede.ufrrj.br/jspui/handle/tede/761 |
identifier_str_mv |
CALDAS, Saulo Andrade. Aspectos cl?nico patol?gicos e laboratoriais do envenenamento experimental por Bothrops alternatus em bovinos. 2008. 111 f. Disserta??o (Mestrado em Ci?ncias Veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2008. |
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https://tede.ufrrj.br/jspui/handle/tede/761 |
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Universidade Federal Rural do Rio de Janeiro |
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Programa de P?s-Gradua??o em Ci?ncias Veterin?rias |
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UFRRJ |
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Brasil |
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Instituto de Veterin?ria |
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Universidade Federal Rural do Rio de Janeiro |
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