Detec??o de Anaplasma platys em c?es e em carrapatos: padroniza??o de qPCR e an?lise epidemiol?gica no Estado do Rio de Janeiro, Brasil e na regi?o ocidental de Cuba

Detalhes bibliográficos
Ano de defesa: 2016
Autor(a) principal: Silva, Claudia Bezerra da lattes
Orientador(a): Massard, Carlos Luiz lattes
Banca de defesa: Coelho, Irene da Silva, Guedes, Daniel da Silva, Barreira, Jairo Dias, Macieira, Daniel Barros
Tipo de documento: Tese
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal Rural do Rio de Janeiro
Programa de Pós-Graduação: Programa de P?s-Gradua??o em Ci?ncias Veterin?rias
Departamento: Instituto de Veterin?ria
País: Brasil
Palavras-chave em Português:
Anaplasma platys
c?es
diagn?stico molecular
qPCR
nested PCR
filogenia
Rhipicephalus sanguineus sensu lato
Palavras-chave em Inglês:
dogs
molecular diagnostic
phylogeny
Área do conhecimento CNPq:
Medicina Veterin?ria
Link de acesso: https://tede.ufrrj.br/jspui/handle/jspui/2107
Resumo: and investigate the circulation of this agent in dogs in the Itaguai microregion, Rio de Janeiro, Brazil, and dogs and ticks in two provinces of the island of Cuba, analyzing epidemiological aspects associated with infections caused by this bacterium in dogs. A new real-time polymerase chain reaction method (qPCR) was patterned to target the citrate synthase gene (gltA) for the identification of A. platys in naturally infected dogs. The primers and probe were designed to amplify a fragment of 84 base pairs based on gltA gene sequences of A. platys available in GenBank. 186 blood samples of dogs from Itaguai microregion, Rio de Janeiro, Brazil, were tested by qPCR. The same samples were tested by cytology and nested polymerase chain reaction (nPCR, 16S rDNA) to determine the performance of qPCR front of these techniques. 17.20% of the samples tested positive by qPCR were significantly more than that detected by nPCR (13.98%). The qPCR technique was more specific than cytology, due to false-positive results obtained by optical microscopy. The prevalence of A. platys in dogs from Itaguai microregion was 14.4%. Dogs less than six months, infested by ticks, that spend the most of the time restrict to domestic environment and without shelter are factors associated with infection by this hemoparasite in dogs in the study area. During research, A. platys held in Cuba, 100 blood samples were collected from residents dogs in four cities located in the provinces of Havana and Mayabeque. When inspecting the animals, found ticks were collected, identified and carefully grouped, forming a total of 49 pools. DNA extracted from blood samples from dogs and ticks were subjected nPCR (16S rDNA). Positive samples in nPCR were also subjected to conventional PCR (gltA gene), and the products were sequenced. Only the species Rhipicephalus sanguineus sensu lato was found in Cuban dogs and 10.2% (n=5/49) of these ticks added to 16.0% (n=16/100) dogs were considered positive for A. platys. All sequences analyzed of the gltA and 16S rDNA genes, respectively, showed a 99-100% identity with sequences from A. platys reported in other countries. Phylogenetic analysis showed two clusters defined for the 16S rDNA gene and three clusters defined for the gltA gene. Based on the gltA gene, the deduced amino acid sequence showed two points of non-synonymous mutations at positions 88 and 168 compared to the reference sequence DQ525687. A preliminary study on the epidemiological aspects associated with infection with A. platys showed no statistical association with the variables studied (p> 0.05). This study also to report the first evidence of A. platys in both dogs and ticks in Cuba also presents for the first time the development of a new qPCR method that contributes to the advancement of research involving A. platys. The epidemiological study in Brazil allowed us to identify significant factors in the occurrence of canine anaplasmosis, while in Cuba, it can be concluded that more research is needed to assess what the deciding factors in the transmission and spread of A. platys in that country.
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spelling Massard, Carlos Luiz257.781.297-34http://lattes.cnpq.br/7743112049924654Santos, Huarrisson Azevedo983.833.295-04http://lattes.cnpq.br/7743112049924654Coelho, Irene da SilvaGuedes, Daniel da SilvaBarreira, Jairo DiasMacieira, Daniel Barros104.963.487-01http://lattes.cnpq.br/1386096108167039Silva, Claudia Bezerra da2017-10-19T13:49:16Z2016-03-11SILVA, Claudia Bezerra da. Detec??o de Anaplasma platys em c?es e em carrapatos: padroniza??o de qPCR e an?lise epidemiol?gica no Estado do Rio de Janeiro, Brasil e na regi?o ocidental de Cuba. 2016. 109 f. Tese (Doutorado em Ci?ncias veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 20116.https://tede.ufrrj.br/jspui/handle/jspui/2107and investigate the circulation of this agent in dogs in the Itaguai microregion, Rio de Janeiro, Brazil, and dogs and ticks in two provinces of the island of Cuba, analyzing epidemiological aspects associated with infections caused by this bacterium in dogs. A new real-time polymerase chain reaction method (qPCR) was patterned to target the citrate synthase gene (gltA) for the identification of A. platys in naturally infected dogs. The primers and probe were designed to amplify a fragment of 84 base pairs based on gltA gene sequences of A. platys available in GenBank. 186 blood samples of dogs from Itaguai microregion, Rio de Janeiro, Brazil, were tested by qPCR. The same samples were tested by cytology and nested polymerase chain reaction (nPCR, 16S rDNA) to determine the performance of qPCR front of these techniques. 17.20% of the samples tested positive by qPCR were significantly more than that detected by nPCR (13.98%). The qPCR technique was more specific than cytology, due to false-positive results obtained by optical microscopy. The prevalence of A. platys in dogs from Itaguai microregion was 14.4%. Dogs less than six months, infested by ticks, that spend the most of the time restrict to domestic environment and without shelter are factors associated with infection by this hemoparasite in dogs in the study area. During research, A. platys held in Cuba, 100 blood samples were collected from residents dogs in four cities located in the provinces of Havana and Mayabeque. When inspecting the animals, found ticks were collected, identified and carefully grouped, forming a total of 49 pools. DNA extracted from blood samples from dogs and ticks were subjected nPCR (16S rDNA). Positive samples in nPCR were also subjected to conventional PCR (gltA gene), and the products were sequenced. Only the species Rhipicephalus sanguineus sensu lato was found in Cuban dogs and 10.2% (n=5/49) of these ticks added to 16.0% (n=16/100) dogs were considered positive for A. platys. All sequences analyzed of the gltA and 16S rDNA genes, respectively, showed a 99-100% identity with sequences from A. platys reported in other countries. Phylogenetic analysis showed two clusters defined for the 16S rDNA gene and three clusters defined for the gltA gene. Based on the gltA gene, the deduced amino acid sequence showed two points of non-synonymous mutations at positions 88 and 168 compared to the reference sequence DQ525687. A preliminary study on the epidemiological aspects associated with infection with A. platys showed no statistical association with the variables studied (p> 0.05). This study also to report the first evidence of A. platys in both dogs and ticks in Cuba also presents for the first time the development of a new qPCR method that contributes to the advancement of research involving A. platys. The epidemiological study in Brazil allowed us to identify significant factors in the occurrence of canine anaplasmosis, while in Cuba, it can be concluded that more research is needed to assess what the deciding factors in the transmission and spread of A. platys in that country.platys, e investigar a circula??o deste agente em c?es na microrregi?o de Itagua?, Rio de Janeiro, Brasil, e c?es e carrapatos em duas prov?ncias da ilha de Cuba, analisando aspectos epidemiol?gicos associados ? infec??o causada por esta bact?ria em c?es. Um novo m?todo de rea??o em cadeia da polimerase em tempo real (qPCR) foi padronizado com alvo no gene citrato sintase (gltA) para a identifica??o de A. platys em c?es naturalmente infectados. Os oligoiniciadores e a sonda foram desenhados para amplificar um fragmento de 84 pares de base baseado em sequ?ncias do gene gltA de A. platys dispon?veis no GenBank. 186 amostras de sangue de c?es da microrregi?o de Itagua?, Rio de Janeiro, Brasil, foram testados pela qPCR. As mesmas amostras foram testadas pela citologia e rea??o em cadeia da polimerase nested (nPCR, 16S rDNA) para determinar o desempenho da qPCR frente ? essas t?cnicas. 17,20% das amostras testadas pela qPCR foram positivas, significativamente mais do que detectado pela nPCR (13,98%). A t?cnica de qPCR foi mais espec?fica que a citologia, em virtude dos resultados falsopositivos obtidos pela microscopia ?ptica. A preval?ncia de A. platys em c?es da microrregi?o de Itagua? foi de 14,4%. C?es com menos de seis meses, infestados por carrapatos, que possam maior tempo restrito ao ambiente dom?stico e sem abrigo s?o fatores associados a infec??o por este hemoparasito em c?es na regi?o do estudo. Durante investiga??o de A. platys realizada em Cuba, 100 amostras de sangue foram coletadas de c?es residentes em quatro cidades localizadas nas prov?ncias de Habana e Mayabeque. Ao inspecionar os animais, carrapatos encontrados foram coletados, identificados e criteriosamente agrupados, formando um total de 49 pools. Amostras de DNA extra?das do sangue dos c?es e de carrapatos foram submetidas a nPCR (16S rDNA). Amostras positivas na nPCR foram tamb?m submetidas a PCR convencional (gene gltA), e os produtos foram sequenciados. Somente a esp?cie Rhipicephalus sanguineus sensu lato foi encontrada em c?es cubanos, e 10,2% (n=5/49) desses carrapatos somado aos 16,0% (n=16/100) de c?es foram considerados positivos para A. platys. Todas as sequ?ncias analisadas dos genes gltA e 16S rDNA, respectivamente, mostraram uma identidade de 99-100% com sequ?ncias de A. platys reportadas em outros pa?ses. A an?lise filogen?tica mostrou dois clusters definidos para o gene 16S rDNA e tr?s clusters definidos para o gene gltA. Com base no gene gltA, a sequ?ncia de amino?cidos deduzidos demonstrou dois pontos de muta??es n?o-sin?nimas nas posi??es 88 e 168 comparados com sequ?ncia de refer?ncia DQ525687. Um estudo preliminar sobre os aspectos epidemiol?gicos associados com a infec??o por A. platys demonstrou nenhuma associa??o estat?stica com as vari?veis avaliadas (p > 0,05). O presente estudo al?m de relatar a primeira evid?ncia de A. platys em ambos c?es e carrapatos em Cuba, tamb?m apresenta pela primeira vez o desenvolvimento de um novo m?todo de qPCR que contribui para o avan?o da pesquisa envolvendo A. platys. O estudo epidemiol?gico realizado no Brasil permitiu identificar fatores importantes na ocorr?ncia da anaplasmose canina, enquanto em Cuba, pode-se concluir que mais investiga??es s?o necess?rias para avaliar quais os fatores decisivos na transmiss?o e dispers?o de A. platys nesse pa?s.Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2017-10-19T13:49:16Z No. of bitstreams: 1 2016 - Claudia Bezerra da Silva.pdf: 8032175 bytes, checksum: ef71dd2a0e7801e9000e116c822a3a00 (MD5)Made available in DSpace on 2017-10-19T13:49:16Z (GMT). No. of bitstreams: 1 2016 - Claudia Bezerra da Silva.pdf: 8032175 bytes, checksum: ef71dd2a0e7801e9000e116c822a3a00 (MD5) Previous issue date: 2016-03-11CAPES - Coordena??o de Aperfei?oamento de Pessoal de N?vel Superiorapplication/pdfhttps://tede.ufrrj.br/retrieve/6195/2016%20-%20Claudia%20Bezerra%20da%20Silva.pdf.jpghttps://tede.ufrrj.br/retrieve/21126/2016%20-%20Claudia%20Bezerra%20da%20Silva.pdf.jpghttps://tede.ufrrj.br/retrieve/27403/2016%20-%20Claudia%20Bezerra%20da%20Silva.pdf.jpghttps://tede.ufrrj.br/retrieve/33842/2016%20-%20Claudia%20Bezerra%20da%20Silva.pdf.jpghttps://tede.ufrrj.br/retrieve/40236/2016%20-%20Claudia%20Bezerra%20da%20Silva.pdf.jpghttps://tede.ufrrj.br/retrieve/46612/2016%20-%20Claudia%20Bezerra%20da%20Silva.pdf.jpghttps://tede.ufrrj.br/retrieve/52982/2016%20-%20Claudia%20Bezerra%20da%20Silva.pdf.jpghttps://tede.ufrrj.br/retrieve/59394/2016%20-%20Claudia%20Bezerra%20da%20Silva.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Ci?ncias Veterin?riasUFRRJBrasilInstituto de Veterin?riaCap?tulo I VALIENTE-ECHEVERR?A, F.; LE?N, U.; GUTJAHR, C.; AZ?CAR, T. 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QUROLLO, B.A.; CHANDRASHEKAR, R.; HEGARTY, B.C.; BEALL, M.J.; STILLMAN, B.A.; LIU, J.; THATCHER, B.; PULTORAK, E.; CERRITO, B.; WALSH, M.; BREITSCHWERDT, E.B. A serological survey of tick-borne pathogens in dogs in North America and the Caribbean as assessed by Anaplasma phagocytophilum, A. platys, Ehrlichia canis, E. chaffeensis, E. ewingii, and Borrelia burgdorferi species-specific peptides. Infect. Ecology Epidemiol. v.4, p.1-12, 2014. RAMOS, C.A.N.; RAMOS, R.A.N.; ARA?JO, F.R.; GUEDES JR., D.S.; SOUZA, I.I.F.; ONO, T.M.; VIEIRA, A.S.; PIMENTEL, D.S.; ROSAS, E.O.; FAUSTINO, M.A.G.; ALVES, L.C. Comparison of nested-PCR with blood smear examination in detection of Ehrlichia canis and Anaplasma platys in dogs. Rev. Bras. Parasitol. Vet. v.18, p.58-62, 2009. ROMERO, R.G. Estudios del Caribe en Venezuela. Universidad Central de Venezuela, Consejo de Desarrollo Cient?fico y Human?stico. 1988. 219p. ROJAS, A.; ROJAS, D.; MONTENEGRO, V.; GUTI?RREZ, R.; YASUR-LANDAUC, D.; BANETH, G. Vector-borne pathogens in dogs from Costa Rica: first molecular description of Babesia vogeli and Hepatozoon canis infections with a high prevalence of monocytic ehrlichiosis and the manifestations of co-infection. Vet. Parasitol. v.199, p.3-4, 2014. SANGER, F.; NICKLEN, S.; COULSON, A.R. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA v.74, p.5463-5467, 1977. SANOGO, Y.O.; INOKUMA, H.; PAROLA, P.; BROUQUI, P.; DAVOUST, B.; CAMICAS, J-L. First evidence of Anaplasma platys in Rhipicephalus sanguineus (Acari: Ixodida) collected from dogs in Africa. Onderstepoort J. Vet. v.70, p.205-212, 2003. SANTAMARIA, A.; CALZADA, J.E.; SALDA?A, A.; YABSLEY, M.J.; GOTTDENKER, N.L. Molecular diagnosis and species identification of Ehrlichia and Anaplasma infections in dogs from Panama, Central America. Vector-Borne Zoon. Dis. v.14, p.368-370, 2014. SCHNEIDER, R. A verdade sobre Cuba. Edicoes Loyola. 1992. 136 p. SILVA, G.C.F.; BENITEZ, N.A.; GIROTTO, A.; TARODA, A.; VIDOTTO, M.C.; GARCIA J.L.; FREITAS, J.C.; ARLINGTON, S.H.; VIDOTTO, O. Occurrence of Ehrlichia canis and Anaplasma platys in household dogs from northern Parana. Rev. Bras. Parasitol. Vet. v.21, p.379-385, 2012. SIMPSON, R.M.; GAUNT, S.D.; HAIR, J.A.; KOCAN, K.M.; HENK, W.G.; CASEY, H.W.; Evaluation of Rhipicephalus sanguineus as a potential biologic vector of Ehrlichia platys. Am. J. Vet. Res. v.52, p.1537-1541, 1991. 100 TAMURA, K.; STECHER, G.; PETERSON, D.; FILIPSKI, A.; KUMAR, S. MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Mol. Biol. Evol. v.30, p2725-2729, 2013. TOMMASI, A.S.; BANETH, G.; BREITSCHWERDT, E.B.; STANNECK, D.; DANTASTORRES, F.; OTRANTO, D. CAPRARIIS, D. Anaplasma platys in bone marrow megakaryocytes of young dogs. J. Clin. Microbiol. v.52, p.2231-2234, 2014. UNVER, A.; RIKIHISA, Y.; KAWAHARA, M.; YAMAMOTO, S. Analysis of 16SrRNA gene sequences of Ehrlichia canis, Anaplasma platys, and Wolbachia species from canine blood in Japan. An. New. York Acad. Sci. v.990, p.692-698, 2003. YABSLEY, M.J.; MCKIBBEN, J.; MACPHERSON, C.N.; CATTAN, P.F.; CHERRY, N.A.; HEGARTY, B.C.; BREITSCHWERDT, E.B.; O?CONNOR, T.; CHANDRASHEKAR, R.; PATERSON, T.; PEREA, M.L.; BALL, G.; FRIESEN, S.; GOEDDE, J.; HENDERSON, B.; SYLVESTER, W. Prevalence of Ehrlichia canis, Anaplasma platys, Babesia canis vogeli, Hepatozoon canis, Bartonella vinsonii berkhoffii, and Rickettsia spp. in dogs from Grenada. Vet. Parasitol. v.151, p.279-285, 2008. YU, X.J.; ZHANG, X.F.; MCBRIDE, J.W.; ZHANG, Y.; WALKER, D.H. Phylogenetic relationships of Anaplasma marginale and ?Ehrlichia platys? to other Ehrlichia species determined by GroEL amino acid sequences. Int. J. Syst. Evol. 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dc.title.por.fl_str_mv Detec??o de Anaplasma platys em c?es e em carrapatos: padroniza??o de qPCR e an?lise epidemiol?gica no Estado do Rio de Janeiro, Brasil e na regi?o ocidental de Cuba
dc.title.alternative.eng.fl_str_mv Detection of Anaplasma platys in dogs and ticks: standardization of qPCR and epidemiological analysis in the State of Rio de Janeiro, Brazil and in western Cuba
title Detec??o de Anaplasma platys em c?es e em carrapatos: padroniza??o de qPCR e an?lise epidemiol?gica no Estado do Rio de Janeiro, Brasil e na regi?o ocidental de Cuba
spellingShingle Detec??o de Anaplasma platys em c?es e em carrapatos: padroniza??o de qPCR e an?lise epidemiol?gica no Estado do Rio de Janeiro, Brasil e na regi?o ocidental de Cuba
Silva, Claudia Bezerra da
dogs
molecular diagnostic
phylogeny
Anaplasma platys
c?es
diagn?stico molecular
qPCR
nested PCR
filogenia
Rhipicephalus sanguineus sensu lato
Medicina Veterin?ria
title_short Detec??o de Anaplasma platys em c?es e em carrapatos: padroniza??o de qPCR e an?lise epidemiol?gica no Estado do Rio de Janeiro, Brasil e na regi?o ocidental de Cuba
title_full Detec??o de Anaplasma platys em c?es e em carrapatos: padroniza??o de qPCR e an?lise epidemiol?gica no Estado do Rio de Janeiro, Brasil e na regi?o ocidental de Cuba
title_fullStr Detec??o de Anaplasma platys em c?es e em carrapatos: padroniza??o de qPCR e an?lise epidemiol?gica no Estado do Rio de Janeiro, Brasil e na regi?o ocidental de Cuba
title_full_unstemmed Detec??o de Anaplasma platys em c?es e em carrapatos: padroniza??o de qPCR e an?lise epidemiol?gica no Estado do Rio de Janeiro, Brasil e na regi?o ocidental de Cuba
title_sort Detec??o de Anaplasma platys em c?es e em carrapatos: padroniza??o de qPCR e an?lise epidemiol?gica no Estado do Rio de Janeiro, Brasil e na regi?o ocidental de Cuba
author Silva, Claudia Bezerra da
author_facet Silva, Claudia Bezerra da
author_role author
dc.contributor.advisor1.fl_str_mv Massard, Carlos Luiz
dc.contributor.advisor1ID.fl_str_mv 257.781.297-34
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/7743112049924654
dc.contributor.advisor-co1.fl_str_mv Santos, Huarrisson Azevedo
dc.contributor.advisor-co1ID.fl_str_mv 983.833.295-04
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/7743112049924654
dc.contributor.referee1.fl_str_mv Coelho, Irene da Silva
dc.contributor.referee2.fl_str_mv Guedes, Daniel da Silva
dc.contributor.referee3.fl_str_mv Barreira, Jairo Dias
dc.contributor.referee4.fl_str_mv Macieira, Daniel Barros
dc.contributor.authorID.fl_str_mv 104.963.487-01
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/1386096108167039
dc.contributor.author.fl_str_mv Silva, Claudia Bezerra da
contributor_str_mv Massard, Carlos Luiz
Santos, Huarrisson Azevedo
Coelho, Irene da Silva
Guedes, Daniel da Silva
Barreira, Jairo Dias
Macieira, Daniel Barros
dc.subject.eng.fl_str_mv dogs
molecular diagnostic
phylogeny
topic dogs
molecular diagnostic
phylogeny
Anaplasma platys
c?es
diagn?stico molecular
qPCR
nested PCR
filogenia
Rhipicephalus sanguineus sensu lato
Medicina Veterin?ria
dc.subject.por.fl_str_mv Anaplasma platys
c?es
diagn?stico molecular
qPCR
nested PCR
filogenia
Rhipicephalus sanguineus sensu lato
dc.subject.cnpq.fl_str_mv Medicina Veterin?ria
description and investigate the circulation of this agent in dogs in the Itaguai microregion, Rio de Janeiro, Brazil, and dogs and ticks in two provinces of the island of Cuba, analyzing epidemiological aspects associated with infections caused by this bacterium in dogs. A new real-time polymerase chain reaction method (qPCR) was patterned to target the citrate synthase gene (gltA) for the identification of A. platys in naturally infected dogs. The primers and probe were designed to amplify a fragment of 84 base pairs based on gltA gene sequences of A. platys available in GenBank. 186 blood samples of dogs from Itaguai microregion, Rio de Janeiro, Brazil, were tested by qPCR. The same samples were tested by cytology and nested polymerase chain reaction (nPCR, 16S rDNA) to determine the performance of qPCR front of these techniques. 17.20% of the samples tested positive by qPCR were significantly more than that detected by nPCR (13.98%). The qPCR technique was more specific than cytology, due to false-positive results obtained by optical microscopy. The prevalence of A. platys in dogs from Itaguai microregion was 14.4%. Dogs less than six months, infested by ticks, that spend the most of the time restrict to domestic environment and without shelter are factors associated with infection by this hemoparasite in dogs in the study area. During research, A. platys held in Cuba, 100 blood samples were collected from residents dogs in four cities located in the provinces of Havana and Mayabeque. When inspecting the animals, found ticks were collected, identified and carefully grouped, forming a total of 49 pools. DNA extracted from blood samples from dogs and ticks were subjected nPCR (16S rDNA). Positive samples in nPCR were also subjected to conventional PCR (gltA gene), and the products were sequenced. Only the species Rhipicephalus sanguineus sensu lato was found in Cuban dogs and 10.2% (n=5/49) of these ticks added to 16.0% (n=16/100) dogs were considered positive for A. platys. All sequences analyzed of the gltA and 16S rDNA genes, respectively, showed a 99-100% identity with sequences from A. platys reported in other countries. Phylogenetic analysis showed two clusters defined for the 16S rDNA gene and three clusters defined for the gltA gene. Based on the gltA gene, the deduced amino acid sequence showed two points of non-synonymous mutations at positions 88 and 168 compared to the reference sequence DQ525687. A preliminary study on the epidemiological aspects associated with infection with A. platys showed no statistical association with the variables studied (p> 0.05). This study also to report the first evidence of A. platys in both dogs and ticks in Cuba also presents for the first time the development of a new qPCR method that contributes to the advancement of research involving A. platys. The epidemiological study in Brazil allowed us to identify significant factors in the occurrence of canine anaplasmosis, while in Cuba, it can be concluded that more research is needed to assess what the deciding factors in the transmission and spread of A. platys in that country.
publishDate 2016
dc.date.issued.fl_str_mv 2016-03-11
dc.date.accessioned.fl_str_mv 2017-10-19T13:49:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, Claudia Bezerra da. Detec??o de Anaplasma platys em c?es e em carrapatos: padroniza??o de qPCR e an?lise epidemiol?gica no Estado do Rio de Janeiro, Brasil e na regi?o ocidental de Cuba. 2016. 109 f. Tese (Doutorado em Ci?ncias veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 20116.
dc.identifier.uri.fl_str_mv https://tede.ufrrj.br/jspui/handle/jspui/2107
identifier_str_mv SILVA, Claudia Bezerra da. Detec??o de Anaplasma platys em c?es e em carrapatos: padroniza??o de qPCR e an?lise epidemiol?gica no Estado do Rio de Janeiro, Brasil e na regi?o ocidental de Cuba. 2016. 109 f. Tese (Doutorado em Ci?ncias veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 20116.
url https://tede.ufrrj.br/jspui/handle/jspui/2107
dc.language.iso.fl_str_mv por
language por
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Safety and efficacy of doxycycline in the treatment of rosacea. Clin., Cosm. Invest. Dermatol. v.2, p.129-140, 2009. VERLENGIA, R.; CURI, R.; BEVILACQUA, E.; NEWSHOLME, P. An?lise de RNA, prote?na
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