Avalia??o da genotoxicidade e antigenotoxicidade da astaxantina atrav?s do ensaio Cometa em c?lulas de mam?fero in vitro.

• Ano de defesa: 2013
• Autor(a) principal: Ferreira, Micheli de Moraes
• Orientador(a): Abrantes, Shirley de Mello Pereira
• Banca de defesa: Abrantes, Shirley de Mello Pereira , Azevedo-Meleiro, Cristiane Hess de , Silva, Marco Antonio Mota da
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal Rural do Rio de Janeiro
• Programa de Pós-Graduação: Programa de P?s-Gradua??o em Ci?ncia e Tecnologia de Alimentos
• Departamento: Instituto de Tecnologia
• País: Brasil
• Palavras-chave em Português: Caroten?ides; Genotoxicidade; Ensaio cometa; Comet assays
• Palavras-chave em Inglês: Carotenoids; Genotoxicity
• Área do conhecimento CNPq: Ci?ncia e Tecnologia de Alimentos
• Link de acesso: https://tede.ufrrj.br/jspui/handle/jspui/5443

The color and appearance of food are the first fundamental attributes, if not more important, to be evaluated by consumers at the time of its acquisition. The food can be more nutritious, safer and more economical, however, if not attractive, its acquisition will not occur. The salmon is basically a white fish that becomes pink by eating shrimp. The stored red pigment present in muscle or shrimp in the shell and accumulates in adipose tissue is acquired through ingestion algae and unicellular organisms by the shrimps from the sea. Carotenoids used in food, pharmaceutical, cosmetics and food are natural colorants responsible for yellow, orange and red. The raised salmon aquaculture has no access to the bodies mentioned above, however, it is added to their feed astaxanthin (ATX), a substance that gives a pinkish color to your meat. ATX (3,3 '-dihydroxy-beta, beta-carotene-4, 4'-dione) is a oxygenated carotenoid pigment that confers the characteristic reddish-pink coloration of certain fish, crustaceans, birds and microorganisms. The ATX has potent activity in scavenging free radicals and protecting against lipid peroxidation and the damage caused by oxidation of cell membranes and tissues. In this study we investigated in human blood cells in vitro genotoxicity and protective effect or antigenotoxicity ATX induction of oxidative DNA damage caused by hydrogen peroxide (H2O2). ATX (0.1 to 5 ?M) was not cytotoxic in human blood cells are cytotoxic in the range 10-1000 ?M inducing 1 to 10% mortality when compared to control cell-solvent dimethylsulfoxide (DMSO 5%). The ATX Comet assays performed did not induce DNA damage (p> 0.1) on human blood cells tested in the range 0.1 to 500 ?M compared to the 5% DMSO. H2O2 (12.5 to 2000 ?M) was not cytotoxic and did not cause cell lethality in human blood cells but was genotoxic (p <0.05), causing damage to DNA. In two experiments independently performed, the pre-treatment of cells in vitro human blood for 2h the ATX (25, 50, 100, 250 and 500 ?M) caused a highly significant reduction (p <0.01) caused by genotoxic 200 and 2000 ?M H2O2 for 10 min. Through this study we demonstrated the protective effect of ATX on the induction of oxidative damage to DNA caused by. H2O2 in human blood cells in vitro.