Extra??o e caracteriza??o do ?leo essencial de tomilho (Thymus vulgaris)

Detalhes bibliográficos
Ano de defesa: 2013
Autor(a) principal: Rocha, B?rbara Costa Antunes da lattes
Orientador(a): Coelho, Gerson Luiz Vieira lattes
Banca de defesa: Moth?, Cheila Gon?alves, Carbonezi, Lidilhone Hamerski
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal Rural do Rio de Janeiro
Programa de Pós-Graduação: Programa de P?s-Gradua??o em Engenharia Qu?mica
Departamento: Instituto de Tecnologia
País: Brasil
Palavras-chave em Português:
Thymus vulgaris
hidrodestila??o
extra??o com solvente
extra??o com flu?do supercr?tico
microextra??o em fase s?lida
Palavras-chave em Inglês:
Thymus vulgaris
hydrodistillation
solvent extraction
solid-phase microextraction
supercritical fluid extraction
Área do conhecimento CNPq:
Engenharia Qu?mica
Link de acesso: https://tede.ufrrj.br/jspui/handle/jspui/3478
Resumo: This work aim to study the chemical composition of leaves of Thymus vulgaris (thyme) using different extraction techniques. The extraction techniques used were: hydrodistillation (HD), solvent extraction (SE), supercritical fluid extraction (SFE) and solid phase microextraction (SPME). The identification of the components was performed using GC-MS. Some variables that can influence the extraction process were investigated, such as temperature profile, extraction time and choice of fiber for SPME and pressure and extraction time for SFE. The extraction methods used were effective for identifying key components present in thyme, but with some qualitative and quantitative differences. Quantitatively, the major components in all techniques were monoterpenes and sesquiterpenes. Among the conventional methods, extraction solvent obtained a higher amount of extracted components (30) when compared with hydrodistillation, with only 24 compounds. The solvent extraction showed higher concentrations of some components in Thyumus vulgaris leaves when compared with hydrodistillation. Comparing the supercritical fluid extraction and solvent extraction, the first was more efficient because obtained a greater range of extracted compounds (33) in a shorter period of time (60 min). Furthermore, the extracts obtained by SFE are free of solvent, which increases the purity/quality of the oil. The SPME is an extraction procedure easier and faster (30min), besides being even able to identify a greater number of compounds of T. vulgaris (47), when compared to other methods. Moreover, the solid phase microextraction indicated the presence of compounds ?-terpinene, ?- Cubebene and Cadalene. These three compounds were not mentioned in others researches with Thymus vulgaris.
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spelling Coelho, Gerson Luiz Vieira376.071.627-04http://lattes.cnpq.br/3629371878927592Kawase, K?tia Yuri FaustaMoth?, Cheila Gon?alvesCarbonezi, Lidilhone Hamerski059.272.887-07http://lattes.cnpq.br/4399074146642454Rocha, B?rbara Costa Antunes da2020-04-30T19:33:51Z2013-02-22ROCHA, B?rbara Costa Antunes da. Extra??o e caracteriza??o do ?leo essencial de tomilho (Thymus vulgaris), 2013. 91 f. Disserta??o (Mestrado em Engenharia Qu?mica) - Instituto de Tecnologia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2013.https://tede.ufrrj.br/jspui/handle/jspui/3478This work aim to study the chemical composition of leaves of Thymus vulgaris (thyme) using different extraction techniques. The extraction techniques used were: hydrodistillation (HD), solvent extraction (SE), supercritical fluid extraction (SFE) and solid phase microextraction (SPME). The identification of the components was performed using GC-MS. Some variables that can influence the extraction process were investigated, such as temperature profile, extraction time and choice of fiber for SPME and pressure and extraction time for SFE. The extraction methods used were effective for identifying key components present in thyme, but with some qualitative and quantitative differences. Quantitatively, the major components in all techniques were monoterpenes and sesquiterpenes. Among the conventional methods, extraction solvent obtained a higher amount of extracted components (30) when compared with hydrodistillation, with only 24 compounds. The solvent extraction showed higher concentrations of some components in Thyumus vulgaris leaves when compared with hydrodistillation. Comparing the supercritical fluid extraction and solvent extraction, the first was more efficient because obtained a greater range of extracted compounds (33) in a shorter period of time (60 min). Furthermore, the extracts obtained by SFE are free of solvent, which increases the purity/quality of the oil. The SPME is an extraction procedure easier and faster (30min), besides being even able to identify a greater number of compounds of T. vulgaris (47), when compared to other methods. Moreover, the solid phase microextraction indicated the presence of compounds ?-terpinene, ?- Cubebene and Cadalene. These three compounds were not mentioned in others researches with Thymus vulgaris.O presente trabalho teve como objetivo estudar a composi??o qu?mica do ?leo essencial de Thymus vulgaris (tomilho) usando diferentes t?cnicas de extra??o. As t?cnicas de extra??o utilizadas foram: hidrodestila??o (HD), extra??o com solvente (SE), extra??o com flu?do supercr?tico (SFE) e microextra??o em fase s?lida (SPME) e a identifica??o dos componentes foi realizada utilizando GC-MS. Foram investigadas algumas vari?veis que podem influenciar no processo de extra??o, tais como perfil de temperatura, tempo de extra??o e escolha da fibra para SPME, e press?o e tempo de extra??o para SFE. Os m?todos de extra??o utilizados mostraram-se eficientes para identifica??o dos principais componentes presentes no tomilho, por?m com algumas diferen?as qualitativas e quantitativas. Quantitativamente, os componentes majorit?rios, em todas as t?cnicas utilizadas, foram monoterpenos e sesquiterpenos. Entre os m?todos convencionais a extra??o com solvente apresentou maior quantidade de componentes extra?dos (30) quando comparado com a hidrodestila??o, que extraiu apenas 24 compostos. A extra??o com solvente tamb?m apresentou maior concentra??o de alguns componentes presentes nas folhas de Thyumus vulgaris quando comparado com a hidrodestila??o. Comparando a extra??o usando CO2 supercr?tico com a extra??o com solvente, a primeira mostrou-se mais eficiente, pois extraiu uma maior quantidade de compostos (33) em um menor per?odo de tempo (60min). Al?m disso, os extratos obtidos atrav?s do CO2 em alta press?o s?o livres de solvente, o que aumenta a pureza/qualidade do ?leo essencial. A SPME, al?m de ser o procedimento de extra??o mais simples foi o mais r?pido (30min), e ainda mostrou-se capaz de identificar um maior n?mero de compostos de T. vulgaris (47) quando comparado aos demais m?todos utilizados. Ademais, a microextra??o em fase s?lida indicou a presen?a dos terpenos ?-terpineno, ?-Cubebene e Cadalene, ainda n?o mencionados em outros trabalhos de pesquisa com o Thymus vulgaris.Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2020-04-30T19:33:50Z No. of bitstreams: 1 2013 - B?rbara Costa Antunes da Rocha.pdf: 3159471 bytes, checksum: 1222526fa53ad68f7d94ab1176db4c06 (MD5)Made available in DSpace on 2020-04-30T19:33:51Z (GMT). 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dc.title.por.fl_str_mv Extra??o e caracteriza??o do ?leo essencial de tomilho (Thymus vulgaris)
dc.title.alternative.eng.fl_str_mv Extraction and characterization of essential oils from Thymus Vulgaris
title Extra??o e caracteriza??o do ?leo essencial de tomilho (Thymus vulgaris)
spellingShingle Extra??o e caracteriza??o do ?leo essencial de tomilho (Thymus vulgaris)
Rocha, B?rbara Costa Antunes da
Thymus vulgaris
hidrodestila??o
extra??o com solvente
extra??o com flu?do supercr?tico
microextra??o em fase s?lida
Thymus vulgaris
hydrodistillation
solvent extraction
solid-phase microextraction
supercritical fluid extraction
Engenharia Qu?mica
title_short Extra??o e caracteriza??o do ?leo essencial de tomilho (Thymus vulgaris)
title_full Extra??o e caracteriza??o do ?leo essencial de tomilho (Thymus vulgaris)
title_fullStr Extra??o e caracteriza??o do ?leo essencial de tomilho (Thymus vulgaris)
title_full_unstemmed Extra??o e caracteriza??o do ?leo essencial de tomilho (Thymus vulgaris)
title_sort Extra??o e caracteriza??o do ?leo essencial de tomilho (Thymus vulgaris)
author Rocha, B?rbara Costa Antunes da
author_facet Rocha, B?rbara Costa Antunes da
author_role author
dc.contributor.advisor1.fl_str_mv Coelho, Gerson Luiz Vieira
dc.contributor.advisor1ID.fl_str_mv 376.071.627-04
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/3629371878927592
dc.contributor.advisor-co1.fl_str_mv Kawase, K?tia Yuri Fausta
dc.contributor.referee1.fl_str_mv Moth?, Cheila Gon?alves
dc.contributor.referee2.fl_str_mv Carbonezi, Lidilhone Hamerski
dc.contributor.authorID.fl_str_mv 059.272.887-07
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/4399074146642454
dc.contributor.author.fl_str_mv Rocha, B?rbara Costa Antunes da
contributor_str_mv Coelho, Gerson Luiz Vieira
Kawase, K?tia Yuri Fausta
Moth?, Cheila Gon?alves
Carbonezi, Lidilhone Hamerski
dc.subject.por.fl_str_mv Thymus vulgaris
hidrodestila??o
extra??o com solvente
extra??o com flu?do supercr?tico
microextra??o em fase s?lida
topic Thymus vulgaris
hidrodestila??o
extra??o com solvente
extra??o com flu?do supercr?tico
microextra??o em fase s?lida
Thymus vulgaris
hydrodistillation
solvent extraction
solid-phase microextraction
supercritical fluid extraction
Engenharia Qu?mica
dc.subject.eng.fl_str_mv Thymus vulgaris
hydrodistillation
solvent extraction
solid-phase microextraction
supercritical fluid extraction
dc.subject.cnpq.fl_str_mv Engenharia Qu?mica
description This work aim to study the chemical composition of leaves of Thymus vulgaris (thyme) using different extraction techniques. The extraction techniques used were: hydrodistillation (HD), solvent extraction (SE), supercritical fluid extraction (SFE) and solid phase microextraction (SPME). The identification of the components was performed using GC-MS. Some variables that can influence the extraction process were investigated, such as temperature profile, extraction time and choice of fiber for SPME and pressure and extraction time for SFE. The extraction methods used were effective for identifying key components present in thyme, but with some qualitative and quantitative differences. Quantitatively, the major components in all techniques were monoterpenes and sesquiterpenes. Among the conventional methods, extraction solvent obtained a higher amount of extracted components (30) when compared with hydrodistillation, with only 24 compounds. The solvent extraction showed higher concentrations of some components in Thyumus vulgaris leaves when compared with hydrodistillation. Comparing the supercritical fluid extraction and solvent extraction, the first was more efficient because obtained a greater range of extracted compounds (33) in a shorter period of time (60 min). Furthermore, the extracts obtained by SFE are free of solvent, which increases the purity/quality of the oil. The SPME is an extraction procedure easier and faster (30min), besides being even able to identify a greater number of compounds of T. vulgaris (47), when compared to other methods. Moreover, the solid phase microextraction indicated the presence of compounds ?-terpinene, ?- Cubebene and Cadalene. These three compounds were not mentioned in others researches with Thymus vulgaris.
publishDate 2013
dc.date.issued.fl_str_mv 2013-02-22
dc.date.accessioned.fl_str_mv 2020-04-30T19:33:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv ROCHA, B?rbara Costa Antunes da. Extra??o e caracteriza??o do ?leo essencial de tomilho (Thymus vulgaris), 2013. 91 f. Disserta??o (Mestrado em Engenharia Qu?mica) - Instituto de Tecnologia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2013.
dc.identifier.uri.fl_str_mv https://tede.ufrrj.br/jspui/handle/jspui/3478
identifier_str_mv ROCHA, B?rbara Costa Antunes da. Extra??o e caracteriza??o do ?leo essencial de tomilho (Thymus vulgaris), 2013. 91 f. Disserta??o (Mestrado em Engenharia Qu?mica) - Instituto de Tecnologia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2013.
url https://tede.ufrrj.br/jspui/handle/jspui/3478
dc.language.iso.fl_str_mv por
language por
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