Neorickettsia risticii: aspectos soro-epidemiol?gicos e estudo da malacofauna em propriedades com cria??es de equinos no estado do Rio de Janeiro

• Ano de defesa: 2014
• Autor(a) principal: Costa, Renata Lins da
• Orientador(a): Santos, Huarrisson Azevedo
• Banca de defesa: Brandolini, Solange Viana Paschoal Blanco, Guedes Junior, Daniel da Silva, Silva, Jairo Pinheiro da
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal Rural do Rio de Janeiro
• Programa de Pós-Graduação: Programa de P?s-Gradua??o em Ci?ncias Veterin?rias
• Departamento: Instituto de Veterin?ria
• País: Brasil
• Palavras-chave em Português: Neorickettsia risticii; Moluscos l?mnicos; Tremat?deos; Epidemiologia
• Palavras-chave em Inglês: limnics Molluscs; Trematodes; Epidemiology
• Área do conhecimento CNPq: Medicina Veterin?ria
• Link de acesso: https://tede.ufrrj.br/jspui/handle/jspui/2918

This work was developed in properties with creations of horses in the micro-regions of Itaguai and Serrana, both located in the state of Rio de Janeiro. The study aimed to evaluate the role of molluscs and trematodes in Neorickettsia risticii cycle in creations located in the micro-region of Itagua? and analyze the frequency of antibody against N. risticii in horses from Serrana micro-region, standing out the possible associated factors with the seropositivity of animals. Among thirteen properties studied in Itaguai micro-region were collected 410 specimens of limnic molluscs using hand net adapted by fine screen. The molluscs were exposed to the incandescent light (60 w) during 2-4 hours for research of trematodes larval forms. Subsequently, proceeded to molecular detection of N. risticii by Polymerase Chain Reaction in real time (qPCR) in DNA samples of molluscs as in samples of trematodes larval. Many taxons of molluscs were found: 71.70% (294/410) represented by Melanoides tuberculata; 10.97% (45/410) by Pomacea sp.; 3.65% (15/410) by Drepanotrema anatinum; 6.58% (27/410) by Biomphalaria tenagophila; 0.24% (1/410) by B. straminea; 5.60% (23/410) by Physa acuta and 1.21% (5/410) by Family Hydobriidae. A frequency of 3.17% (13/410) of molluscs parasited by trematodes was observed. According to the morphometric and biological characteristics observed in trematodes were classified the following cercariae types: Megalourous cercariae, Pleurolophocercous cercariae and Furcocercous cercariae. No amplification of the target DNA for N. risticii was observed in any DNA samples of molluscs and trematodes analyzed. Regarding to sero-epidemiological study of N. risticii in horses of Serrana micro-region, blood samples from 354 animals were collected, residing at 49 properties of region, by jugular venipuncture in tubes of 5mL without anticoagulant. Blood samples were subjected to centrifugation to obtainment of serums which were stored in freezer at -20?C until analysis moment. For detection of antibodies against N. risticii was used the Immunofluorescence Antibody Test (IFAT), and the samples were considered positive when reacted to N. risticii at 1:50 dilution. Between the municipalities of Petropolis and Teresopolis, was found a frequency of 5.9% of serologically reactive horses. Among the possible factors analyzed, it was found that gender, breed, age, zootechnical and sanitary management, presence of snails and flooded areas did not present association with seropositivity of horses by N. risticii (p>0.05). The region of origin or provenance of the animal was the only factor associated (p<0.05) with seropositivity for N. risticii in the area studied. The presence of animals serologically reactive to N. risticii in Petropolis and Teresopolis indicate the possible movement of the agent in one more geographical area of Brazil. Therefore, the research of molluscs and trematodes that sheltering the bacterium N. risticii may help in understanding the chain of Equine Monocytic Ehrlichiosis (EME) transmission, especially in regions with serological evidence of affected animals. Moreover, the study of the epidemiology aspects of this disease in horses creations of Rio de Janeiro