Caracteriza??o de mel de Apis mellifera pelo seu perfil em subst?ncias fen?licas por cromatografia l?quida de alta efici?ncia e avalia??o da atividade biol?gica
| Ano de defesa: | 2004 |
|---|---|
| Autor(a) principal: |
Lianda, Regina Lucia Pelachim
 |
| Orientador(a): |
Castro, Rosane Nora
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| Banca de defesa: | , , , |
| Tipo de documento: | Dissertação |
| Tipo de acesso: | Acesso aberto |
| Idioma: | por |
| Instituição de defesa: |
Universidade Federal Rural do Rio de Janeiro
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| Programa de Pós-Graduação: |
Programa de P?s-Gradua??o em Qu?mica
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| Departamento: |
Instituto de Ci?ncias Exatas
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| País: |
Brasil
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| Palavras-chave em Português: | |
| Palavras-chave em Inglês: | |
| Área do conhecimento CNPq: | |
| Link de acesso: | https://tede.ufrrj.br/jspui/handle/jspui/4221 |
Resumo: | This work describes the identification of phenolic acids and flavonoids present in five samples of wild honeys and six of citrus honey from different geographical regions. The honey samples were analyzed by high-performance liquid chromatography with a diode-array detector and UV-Vis. The HPLC phenolic profiles and the UV spectra obtained by diode-array detector at ? 250-380 nm, allowed to identify phenolic acids and flavonoids in approximated 40 minutes. In the wild honey samples were identified the gallic, vanillic, para-coumaric, para-methoxy benzoic, para-methoxy-cinamic, para-hydroxy-benzoic, protocatecuic and sinapic acids, and morin flavonoid, while the citrus honey samples were identified mainly gallic, vanillic, para-coumaric, para-methoxy-cinamic, para-hydroxybenzoic, protocatecuic, syringic, sinapic e cinamic acids, and quercetin and rutin flavonoids. In the RLL20 sample (citrus honey ? Taubat?-SP) were identified a nd isolated, in the first time, the morin flavonoid. The HPLC technique has been showed to be efficient and reproductive in the characterization of the phenolic compounds present in eleven honey samples. The chromatographic profiles of the phenolic compounds allowed establishing the composition from monofloral honeys from different regions geographical, because they did not show any major qualitative differences. These compounds would be used as chemical markers for the botanical and/or geographical origin of Apis mellifera honey. Among several compounds contained in honey, the phenolic compounds, such as phenolic acids and flavonoids have been described to contain biological activities, including antibacterial, antioxidant and antitumor activities. Despite of the identification of many phenolic compounds in honeys, the biological activity was carried with the mainly fraction of the phenolic compounds from wild honey from Itarar?-SP. The antitumor activity was tested both in vitro and in vivo on Erlich carcinoma in mouse. The citotoxicity showed moderate antitumor activity in vitro (25% of increase inhibition), however in vivo the results showed significative inhibition of tumor increase and advance of animals survival (67% of the animals didn?t showed tumor after 75 days of treatment). These studies of the biological activity of the extracts of honey were only preliminary but they showed that phenolic compounds might have therapeutic potential. |
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Castro, Rosane Norahttp://lattes.cnpq.br/5479814788308057Castro, Rosane NoraGodoy, Ronoel Luiz de OliveiraEchevarria, AureaSantana, Djalva Maria da N?bregahttp://lattes.cnpq.br/0876721299530164Lianda, Regina Lucia Pelachim2020-11-30T15:52:06Z2004-12-17Lianda, Regina Lucia Pelachim. Caracteriza??o de mel de Apis mellifera pelo seu perfil em subst?ncias fen?licas por cromatografia l?quida de alta efici?ncia e avalia??o da atividade biol?gica. 2004. 142 f. Disserta??o (Programa de P?s-Gradua??o em Qu?mica) - Universidade Federal Rural do Rio de Janeiro, Serop?dica-RJ.https://tede.ufrrj.br/jspui/handle/jspui/4221This work describes the identification of phenolic acids and flavonoids present in five samples of wild honeys and six of citrus honey from different geographical regions. The honey samples were analyzed by high-performance liquid chromatography with a diode-array detector and UV-Vis. The HPLC phenolic profiles and the UV spectra obtained by diode-array detector at ? 250-380 nm, allowed to identify phenolic acids and flavonoids in approximated 40 minutes. In the wild honey samples were identified the gallic, vanillic, para-coumaric, para-methoxy benzoic, para-methoxy-cinamic, para-hydroxy-benzoic, protocatecuic and sinapic acids, and morin flavonoid, while the citrus honey samples were identified mainly gallic, vanillic, para-coumaric, para-methoxy-cinamic, para-hydroxybenzoic, protocatecuic, syringic, sinapic e cinamic acids, and quercetin and rutin flavonoids. In the RLL20 sample (citrus honey ? Taubat?-SP) were identified a nd isolated, in the first time, the morin flavonoid. The HPLC technique has been showed to be efficient and reproductive in the characterization of the phenolic compounds present in eleven honey samples. The chromatographic profiles of the phenolic compounds allowed establishing the composition from monofloral honeys from different regions geographical, because they did not show any major qualitative differences. These compounds would be used as chemical markers for the botanical and/or geographical origin of Apis mellifera honey. Among several compounds contained in honey, the phenolic compounds, such as phenolic acids and flavonoids have been described to contain biological activities, including antibacterial, antioxidant and antitumor activities. Despite of the identification of many phenolic compounds in honeys, the biological activity was carried with the mainly fraction of the phenolic compounds from wild honey from Itarar?-SP. The antitumor activity was tested both in vitro and in vivo on Erlich carcinoma in mouse. The citotoxicity showed moderate antitumor activity in vitro (25% of increase inhibition), however in vivo the results showed significative inhibition of tumor increase and advance of animals survival (67% of the animals didn?t showed tumor after 75 days of treatment). These studies of the biological activity of the extracts of honey were only preliminary but they showed that phenolic compounds might have therapeutic potential.Este trabalho descreve a identifica??o de ?cidos fen?licos e flavon?ides em cinco amostras de mel silvestre e seis amostras de mel laranjeira obtidas de diferentes regi?es geogr?ficas. As amostras de m?is foram analisadas por cromatografia l?quida de alta efici?ncia com detector de arranjo de fotodiodos e UV-vis?vel. A an?lise do perfil cromatogr?fico associado ao estudo dos espectros de ultravioleta acumulados a l 250-380 nm, permitiu identificar os ?cidos fen?licos e flavon?ides em uma ?nica corrida de 40 minutos. Para as amostras de mel silvestre foram identificados os ?cidos g?lico, van?lico, para-cum?rico, para-metoxi-benz?ico, para-metoxi-cin?mico, para-hidroxi-benz?ico, protocatecuico e sin?pico, e o flavon?ide morina, enquanto para as amostras de mel laranjeira foram identificados os ?cidos g?lico, van?lico, para-cum?rico, para-metoxicin?mico, para-hidroxi-benz?ico, protocatecuico, sir?ngico, sin?pico e cin?mico e os flavon?ides quercetina e rutina. Al?m desses ?cidos, para a amostra RLL20 (mel laranjeira de Taubat?-SP) foi isolado e identificado, pela primeira vez, o flavonol morina. A t?cnica de cromatografia l?quida de alta efici?ncia mostrou-se eficiente e reprodutiva na caracteriza??o das subst?ncias fen?licas encontradas nas onze amostras de m?is estudados. O estudo perfil cromatogr?fico para as subst?ncias fen?licas permitiu estabelecer a composi??o para os m?is monoflorais, oriundos de diferentes regi?es geogr?ficas, pois estes n?o mostraram altera??es qualitativas dr?sticas. Estas subst?ncias poder?o vir a ser usadas como marcadores qu?micos para a caracteriza??o da origem bot?nica e/ou geogr?fica do mel de Apis mellifera. Dentre as diversas subst?ncias contidas no mel destacam-se as subst?ncias fen?licas, devido ?s importantes atividades biol?gicas, tais como atividades antimicrobianas, antiinflamat?rias, antiproliferativas e antitumorais. Os ensaios biol?gicos avaliaram a atividade antitumoral in vivo e in vitro da fra??o rica em compostos fen?licos do mel silvestre proveniente da cidade de Itarar?-SP, sobre o crescimento do carcinoma de Erlich em camundongos. A citotoxicidade mostrou-se pouco significativa para a atividade antitumoral in vitro (25% de inibi??o do crescimento), por?m os resultados in vivo mostraram significativa inibi??o do crescimento do tumor e aumento da sobrevida dos animais (67% dos animais ficaram isentos de tumor ap?s 75 dias de tratamento). Os estudos de atividade biol?gica do extrato do mel s?o preliminares, mas revelam-no como potente agente terap?uticoSubmitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2020-11-30T15:52:06Z No. of bitstreams: 1 2004 - Regina Lucia Pelachim Lianda.pdf: 2366342 bytes, checksum: 89b4d56f6a9b7c8bf9da5732a3bb32b5 (MD5)Made available in DSpace on 2020-11-30T15:52:06Z (GMT). No. of bitstreams: 1 2004 - Regina Lucia Pelachim Lianda.pdf: 2366342 bytes, checksum: 89b4d56f6a9b7c8bf9da5732a3bb32b5 (MD5) Previous issue date: 2004-12-17Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPESapplication/pdfhttps://tede.ufrrj.br/retrieve/63300/2004%20-%20Regina%20Lucia%20Pelachim%20Lianda.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Qu?micaUFRRJBrasilInstituto de Ci?ncias ExatasALJADI, A.M. & KAMARUDDIN, M.Y. Isolation and identification of phenolic acids in Malaysian honey with antibacterial properties. Turk J. Med. Sci. 2003, 33, 229-236. 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Flavonoid composition of Tunisian honeys and propolis. Journal Agric. Food Chem. 1997, 45(8), 2824-2829. MARTOS, I.; FERRERES, F.; TOM?S-BARBER?N, F.A. Identification of flavonoids markers for the botanical origin of Eucaliptus honey. Journal Agric. Food Chem . 2000, 48, 1498-1502. MARTOS, I.; FERRERES, F.; YAO, L.H. ; D?ARCY, B.R.; CAFFIN, N.; TOM?SBARBER?N, F.A. Flavonoids in monospecific Eucalyptus honeys from Australia. Journal Agric. Food Chem. 2000a, 48, 4744-4748. MENDES, B. A. & COELHO, E. M. Considera??es sobre caracter?sticas de mel de abelhas ? An?lises e crit?rios de inspe??o. Informe Agropec?ario 1983, 9(106), 56-67. MERKEN, H.M. & GARY, R.B. Measurement of food flavonoids by high-performance liquid chromatography : a Review. Journal of Agricultural and Food Chemistry 2000, 48(3), 577- 599. 139 MEYER, V. R. Practical High-Performance Liquid Chromatography. 2nd, John Wiley & Sons, 1994. MIORIN, P.L.; JUNIOR, N.C.L.; CUSTODIO, A.R.; BRETZ, W.A.; MARCUCCI, M.C. Antibacterial activity of honey and propolis from Apis mellifera and Tetragonisca angustula against Staphylococcus aureus. Journal of Applied Microbiology 2003, 95, 913- 920. MOREIRA, R.F.A.; TRUGO, L.C.; PIETROLUONGO, M.; DE MARIA, C.A.B. Flavor composition of cashew (anacardium occidentale) and marmeleiro (Croton species) honeys. J. Agric. Food Chem . 2002, 50, 7616-7621. NARAYANA, K.R.; REDDY, M.S.; CHALUVADI, M.R.; KRISHNA, D.R. Bioflavonoids classification, pharmacological, biochemical effects and therapeutic potential. Indian Journal of Pharmacology 2001, 33, 2-16. NIJVELDT, R.J.; NOOD, E.V.; HOORN, D.E.C.V.; BOELENS, P.G.; NORREN, K.V.; LEEUWEN, P.A.M.V. Flavonoids: a review of probable mechanisms of action and potential applications. Am. J. Clin. Nutr. 2001, 74, 418-425. PARR, A.J. & BOLWELL, G.P. Phenols in the plant and in man. The potential for possible nutritional enhancement of the diet by modifying the phenol content or profile. Journal Agric. 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TAN, S.T.; WILKINS, A.L.; HOLLAND, P.T.; McGHIE, T.K. 1.Extractives from New Zealand unifloral honeys. 2.Degraded carotenoids and other substances from heather honey. Journal of Agricultural and Food Chemistry 1989a, 37(5), 1217-1221. TERRAB, A.; D?EZ, M.J.; HEREDIA, F.J. Palynological, physico-chemical and colour characterization of Moroccan honeys: I. River red gum (Eucalyptus camaldulensis Dehnh) honey. International Journal of Food Science and Technology 2003, 38, 379-386. TOM?S-BARBER?N, F.A.; FERRERES, F.; BL?ZQUEZ, A.; GARC?A-VIGUERA, C.; TOM?S-LORENTE, F. High-performance liquid chromatography of honey flavonoids. Journal of Chromatography 1993, 634, 41-46. TOM?S-BARBER?N, F.A; FERRERES, F.; GARCIA-VIGUERA, C.; TOM?SLORENTE, F. Flavonoids in honey of different geographical origin. Zeitschrift-fuer Lebensmittel Untersuchung und Forschung 1993a, 196, 38-44. TOM?S-BARBER?N, F.A; FERRERES, F.; TOM?S-LORENTE, F.; GARC?AVIGUERA, C. Hesperetin: A marker of the floral origin of citrus honey. J. Sci. Food Agric. 1993b, 61, 121-123. TOM?S-BARBER?N, F.A; FERRERES, F.; GINER, J.M. A comparative study of hesperetin and methyl anthranilate as markers of the floral origin of citrus honey. J. Sci.Food Agric. 1994, 65, 371-372. TOM?S-BARBER?N, F.A; MARTOS, I.; FERRERES, F.; RADOVIC, B.S.; ANKLAM, E. HPLC flavonoid profiles as markers for the botanical origin of European unifloral honeys. J. Sci.Food Agric. 2001, 81, 485-496. 142 VARGAS, A.C.; LOGUERCIO, A.P.; WITT, N.M.; COSTA, M.M.; SILVA, M.S.S.; VIANA, L.R. Atividade antimicrobiana ?in vitro? de extrato alco?lico de pr?polis. Cienc. Rural 2004, 34 (1), Santa Maria ene./feb. 2004. WANWIMOLRUK, S.; HO, P.; SAVILLE, D.J. Inhibition of human CYP3A4 activity by grapefruit flavonoids, furanocoumarins and related compounds. J. Pharm. Pharmaceut. Sci. 2001, 4(3), 217-227. WHITE, J.W. Physical characteristics of honey. In: CRANE, E. Honey a comprehensive survey. 1975. London: Heinemann, Cap.6, p.207-239. WILKINS, A.L., LU, Y.; MOLAN, P.C. Extractable organic substances from New Zealand unifloral manuka (Leptospermum scoparium) honey. Journal of Apicultural Research 1993, 32 (1), 3-9. WILLIAMS, D.H. & FLEMING, I. Spectroscopic Methods in Organic Chemistry. McGraw-Hill, 5th ed. 1995, p. 329. WINTER, F. C-ring cleavage of flavonoids by human intestinal bacteria. Applied and Environmental Microbiology 1989, 55(5), 1203-1208. WOOTON, M.; EDWARDS, R.A.; FARAJI-HAREMI, R. Effect of accelerated storage conditions on the chemical compostion and properties of australian honeys. 2. Changes in sugar and free amino acid contents. Journal of Apicultural Research 1976, 15(1), 29-34. WULF, L.W. & NAGEL, C.W. Analysis of phenolic acids and flavonoids by high-pressure liquid chromatography. Journal of Chromatography 1976, 116, 271-279. YAO, L.; JIANG, Y.; SINGANUSONG, R.; DATTA, N.; RAYMONT, K. Phenolic acids and abscisic acid in Australian Eucalyptus honeys and their potential for floral authentication. Food Chemistry 2004, 86, 169-177. YAO, L.; JIANG, Y.; D?ARCY, B.; SINGANUSONG, R.; DATTA, N.; CAFFIN, N.; RAYMONT, K. Quantitative high-performance liquid chromatography analyses of flavonoids in Australian Eucalyptus honeys. J. Agric. Food Chem. 2004a, 52, 210-214. ZWAENEPREL, C. Honey: facts and folklore. 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| dc.title.por.fl_str_mv |
Caracteriza??o de mel de Apis mellifera pelo seu perfil em subst?ncias fen?licas por cromatografia l?quida de alta efici?ncia e avalia??o da atividade biol?gica |
| dc.title.alternative.por.fl_str_mv |
Characterization of Apis mellifera honey by highperformance liquid chromatography phenolics compounds profile and biological activity evaluation |
| title |
Caracteriza??o de mel de Apis mellifera pelo seu perfil em subst?ncias fen?licas por cromatografia l?quida de alta efici?ncia e avalia??o da atividade biol?gica |
| spellingShingle |
Caracteriza??o de mel de Apis mellifera pelo seu perfil em subst?ncias fen?licas por cromatografia l?quida de alta efici?ncia e avalia??o da atividade biol?gica Lianda, Regina Lucia Pelachim ?cidos fen?licos Flavon?ides Mel CLAE Atividade Antitumoral Phenolic acids Flavonoids Honey HPLC Antitumor Activity Qu?mica |
| title_short |
Caracteriza??o de mel de Apis mellifera pelo seu perfil em subst?ncias fen?licas por cromatografia l?quida de alta efici?ncia e avalia??o da atividade biol?gica |
| title_full |
Caracteriza??o de mel de Apis mellifera pelo seu perfil em subst?ncias fen?licas por cromatografia l?quida de alta efici?ncia e avalia??o da atividade biol?gica |
| title_fullStr |
Caracteriza??o de mel de Apis mellifera pelo seu perfil em subst?ncias fen?licas por cromatografia l?quida de alta efici?ncia e avalia??o da atividade biol?gica |
| title_full_unstemmed |
Caracteriza??o de mel de Apis mellifera pelo seu perfil em subst?ncias fen?licas por cromatografia l?quida de alta efici?ncia e avalia??o da atividade biol?gica |
| title_sort |
Caracteriza??o de mel de Apis mellifera pelo seu perfil em subst?ncias fen?licas por cromatografia l?quida de alta efici?ncia e avalia??o da atividade biol?gica |
| author |
Lianda, Regina Lucia Pelachim |
| author_facet |
Lianda, Regina Lucia Pelachim |
| author_role |
author |
| dc.contributor.advisor1.fl_str_mv |
Castro, Rosane Nora |
| dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/5479814788308057 |
| dc.contributor.referee1.fl_str_mv |
Castro, Rosane Nora |
| dc.contributor.referee2.fl_str_mv |
Godoy, Ronoel Luiz de Oliveira |
| dc.contributor.referee3.fl_str_mv |
Echevarria, Aurea |
| dc.contributor.referee4.fl_str_mv |
Santana, Djalva Maria da N?brega |
| dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0876721299530164 |
| dc.contributor.author.fl_str_mv |
Lianda, Regina Lucia Pelachim |
| contributor_str_mv |
Castro, Rosane Nora Castro, Rosane Nora Godoy, Ronoel Luiz de Oliveira Echevarria, Aurea Santana, Djalva Maria da N?brega |
| dc.subject.por.fl_str_mv |
?cidos fen?licos Flavon?ides Mel CLAE Atividade Antitumoral |
| topic |
?cidos fen?licos Flavon?ides Mel CLAE Atividade Antitumoral Phenolic acids Flavonoids Honey HPLC Antitumor Activity Qu?mica |
| dc.subject.eng.fl_str_mv |
Phenolic acids Flavonoids Honey HPLC Antitumor Activity |
| dc.subject.cnpq.fl_str_mv |
Qu?mica |
| description |
This work describes the identification of phenolic acids and flavonoids present in five samples of wild honeys and six of citrus honey from different geographical regions. The honey samples were analyzed by high-performance liquid chromatography with a diode-array detector and UV-Vis. The HPLC phenolic profiles and the UV spectra obtained by diode-array detector at ? 250-380 nm, allowed to identify phenolic acids and flavonoids in approximated 40 minutes. In the wild honey samples were identified the gallic, vanillic, para-coumaric, para-methoxy benzoic, para-methoxy-cinamic, para-hydroxy-benzoic, protocatecuic and sinapic acids, and morin flavonoid, while the citrus honey samples were identified mainly gallic, vanillic, para-coumaric, para-methoxy-cinamic, para-hydroxybenzoic, protocatecuic, syringic, sinapic e cinamic acids, and quercetin and rutin flavonoids. In the RLL20 sample (citrus honey ? Taubat?-SP) were identified a nd isolated, in the first time, the morin flavonoid. The HPLC technique has been showed to be efficient and reproductive in the characterization of the phenolic compounds present in eleven honey samples. The chromatographic profiles of the phenolic compounds allowed establishing the composition from monofloral honeys from different regions geographical, because they did not show any major qualitative differences. These compounds would be used as chemical markers for the botanical and/or geographical origin of Apis mellifera honey. Among several compounds contained in honey, the phenolic compounds, such as phenolic acids and flavonoids have been described to contain biological activities, including antibacterial, antioxidant and antitumor activities. Despite of the identification of many phenolic compounds in honeys, the biological activity was carried with the mainly fraction of the phenolic compounds from wild honey from Itarar?-SP. The antitumor activity was tested both in vitro and in vivo on Erlich carcinoma in mouse. The citotoxicity showed moderate antitumor activity in vitro (25% of increase inhibition), however in vivo the results showed significative inhibition of tumor increase and advance of animals survival (67% of the animals didn?t showed tumor after 75 days of treatment). These studies of the biological activity of the extracts of honey were only preliminary but they showed that phenolic compounds might have therapeutic potential. |
| publishDate |
2004 |
| dc.date.issued.fl_str_mv |
2004-12-17 |
| dc.date.accessioned.fl_str_mv |
2020-11-30T15:52:06Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| format |
masterThesis |
| status_str |
publishedVersion |
| dc.identifier.citation.fl_str_mv |
Lianda, Regina Lucia Pelachim. Caracteriza??o de mel de Apis mellifera pelo seu perfil em subst?ncias fen?licas por cromatografia l?quida de alta efici?ncia e avalia??o da atividade biol?gica. 2004. 142 f. Disserta??o (Programa de P?s-Gradua??o em Qu?mica) - Universidade Federal Rural do Rio de Janeiro, Serop?dica-RJ. |
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Lianda, Regina Lucia Pelachim. Caracteriza??o de mel de Apis mellifera pelo seu perfil em subst?ncias fen?licas por cromatografia l?quida de alta efici?ncia e avalia??o da atividade biol?gica. 2004. 142 f. Disserta??o (Programa de P?s-Gradua??o em Qu?mica) - Universidade Federal Rural do Rio de Janeiro, Serop?dica-RJ. |
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Phenolic acids and abscisic acid in Australian Eucalyptus honeys and their potential for floral authentication. Food Chemistry 2004, 86, 169-177. YAO, L.; JIANG, Y.; D?ARCY, B.; SINGANUSONG, R.; DATTA, N.; CAFFIN, N.; RAYMONT, K. Quantitative high-performance liquid chromatography analyses of flavonoids in Australian Eucalyptus honeys. J. Agric. Food Chem. 2004a, 52, 210-214. ZWAENEPREL, C. Honey: facts and folklore. Alberta Beekeepers' Association 1984, Edmonton, Canada, p. 24. |
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