Efeitos sist?micos da implanta??o de peric?rdio ovino tratado pelo glutaralde?do 1% e conservado em glicerina 98% na ves?cula urin?ria de coelhos (Oryctolagus cuniculus)

• Ano de defesa: 2015
• Autor(a) principal: Novaes, Adriano Sodr? Magalh?es
• Orientador(a): Silva, Marta Fernanda Albuquerque da
• Banca de defesa: Silva, Marta Fernanda Albuquerque da, Ferreira, Maria de Lourdes Gon?alves, Nogueira, Vivian de Assun??o, Brito, Marilene de Farias, Corgozinho, K?tia Bar?o
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal Rural do Rio de Janeiro
• Programa de Pós-Graduação: Programa de P?s-Gradua??o em Medicina Veterin?ria (Patologia e Ci?ncias Cl?nicas)
• Departamento: Instituto de Veterin?ria
• País: Brasil
• Palavras-chave em Português: glutaral; glicerol; biopr?teses
• Palavras-chave em Inglês: glycerol; bioprosthesis
• Área do conhecimento CNPq: Medicina Veterin?ria
• Link de acesso: https://tede.ufrrj.br/jspui/handle/jspui/3121

The use of biological implants is growing and gaining considerable impact in veterinary surgery. It is necessary to submit biological membranes to chemical treatment, of which the best known is 98% glycerin, but there is a great concern that the antimicrobial action of glycerin is restricted to nonsporulating and fungi bacteria, allowing transmission of viral agents and sporulated bacteria to the receptor of homologous or heterologous grafts. Since 1969 glutaraldehyde is used in the conservation of biological membranes, being effective against bacterial vegetative forms. The potential risk of systemic toxicity of aldehydes should be considered, and clinical and laboratory tests may be useful for its determination. The objective of this study is to evaluate possible functional or morphological changes in liver, kidney and spleen of rabbits caused by bladder implantation of biological membrane (sheep pericardium) treated by 1% glutaraldehyde and maintained in glycerol 98%. Thirty six animals were divided into six groups, of which Groups 1 to 3 were considered as controls (membranes treated by glycerin 98%) with abate on postoperative days 14, 30 and 60, respectively, and Groups 4, 5 and 6 corresponded to those animals whose implanted membranes were treated with 98% glycerol and 1% glutaraldehyde, post-surgical slaughter at 14, 30 and 60 days. Under general inhalation anesthesia 36 rabbits underwent cystoplasty with replacement of a segment 1 cm x 1 cm of the bladder wall by sheep pericardial patch of the same size that was previously treated with glycerin 98 % for 30 days or 1% glutaraldehyde for 18 days and subsequent treatment by glicerol 98% through a 30 days period. For observation of possible systemic effects of glutaraldehyde 1% absorption, kidney and liver function were evaluated by urea, creatinine, alanine transaminase (ALT), aspartate transferase (AST) and alkaline phosphatase measurements in blood samples collected preoperatively and at the time before slaughter, besides the macro and microscopic analysis of the kidneys, liver and spleen. Food and water intake and excretion in the postoperative period, blood counts and macroscopic evaluation of the peritoneal cavity were performed to rule out prior or concurrent changes. All biochemical values remained in the normal range. Liver function tests variations between the pre-surgical and pre-slaughter collection showed similar pattern in the control and treatment groups; the same was observed in urea levels, but creatinine increased at post-surgical 14 and 30 days only in groups whose membranes were treated with 1% glutaraldehyde (groups 4 and 5), suggesting possible acute effects on this viscera. Macroscopic and microscopic findings did not characterize deleterious effects of glutaraldehyde. It was concluded that treatment of sheep pericardium with glutaraldehyde 1% is safe for use in cystoplasty, regarding kidney, liver and spleen impairment.