Caracteriza??o genot?pica e estudo filogen?tico de Cryptosporidium spp. obtidos de diferentes hospedeiros

• Ano de defesa: 2007
• Autor(a) principal: Huber, Franziska
• Orientador(a): Bomfim, Teresa Cristina Bergamo do
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal Rural do Rio de Janeiro
• Programa de Pós-Graduação: Programa de P?s-Gradua??o em Ci?ncias Veterin?rias
• Departamento: Instituto de Veterin?ria
• País: Brasil
• Palavras-chave em Português: Cryptosporidium; Filogenia; caracteriza??o genot?pica; Brasil
• Palavras-chave em Inglês: Phylogeny; genotypic; characterization
• Área do conhecimento CNPq: Parasitologia
• Link de acesso: https://tede.ufrrj.br/jspui/handle/tede/803

The objectives of the present study was the genetical characterizations of Cryptosporidium spp. from different hosts, realize the sequencing an phylogenetic analysis, including the deposit in GenBank of the first Cryptosporidium sequences of animal origin, from Brazil. There were obtained fecal samples, containing Cryptosporidium oocysts from chiken, ducks, quails and Guinea pigs from a public market localized in Rio de Janeiro city, from dairy calfs maintained at a farm localized in the same city and from dogs and cats maintained at a shelter localized in the city of Nova Igua?u. For the analysis was utilized the Nested-PCR of the extracted DNA from 200?l of fecal suspension. For primary identification of Cryptosporidium species was realized RFLP with enzymes SspI and VspI. DNA samples were sequenced and phylogenetic analysis were conducted. There were diagnosed and sequenced C. baileyi infecting two ducks (DQ855339 and DQ885340) and one quail (DQ885335) and C. melagridis infecting one chicken (DQ885341). The sequences obtained form Cryptosporidium infecting Guinea pigs received accession numbers DQ885337 and DQ885338, both sequences were not identified with known Cryptosporidium species due to the great genetic distance between them and those already available at GenBank, suggesting that it may be a new genotype or species. Parasitizing cats was diagnosed C. felis (DQ885336) and in one dog C. canis (DQ885334). One sample of C. parvum of calf origin was sequenced and received accession number DQ885333. During analysis of RFLP pattern of the nested- PCR product from 18Sr DNA was stated that only C. baileyi has a characteristic digestion pattern. Other Cryptosporidium species should be digested by several other enzymes, for a accurate diagnosis. At phylogenetic analysis was found a greater genetic distance between C. felis and C. canis from Brazil when compared to the reference sequences obtained from GenBank. Based on the phylogenetic groupings, a possible new species of Cryptosporidium from Guinea Pigs calls attention for the existence of new species even in common pet animals. As is the case of the Guinea Pig. The sequences obtained in this study are the first Brazilian sequences of C. baileyi, C. meleagridis, C. felis, C. canis and C. parvum deposited in GenBank.