Morfologia e criopreserva??o do espermatozoide do camar?o branco Litopenaeus schmitti (Crustacea, Decapoda, Penaeidae)

Detalhes bibliográficos
Ano de defesa: 2013
Autor(a) principal: Fernandes, Andrea Bambozzi lattes
Orientador(a): Oshiro, Lidia Miyako Yoshii lattes
Banca de defesa: Keunecke, Karina Annes, Freitas , Simone Patricia Carneiro de, Yasui, George Shigueki, Peixoto, Silvio Ricardo Maurano
Tipo de documento: Tese
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal Rural do Rio de Janeiro
Programa de Pós-Graduação: Programa de P?s-Gradua??o em Zootecnia
Departamento: Instituto de Zootecnia
País: Brasil
Palavras-chave em Português:
Penaeidae
sistema reprodutor masculino
histologia
ultraestrutura
sazonalidade
Palavras-chave em Inglês:
Penaeidae
male reproductive system
histology
ultrastructure
Área do conhecimento CNPq:
Zootecnia
Link de acesso: https://tede.ufrrj.br/jspui/handle/jspui/3456
Resumo: The white shrimp Litopenaeus schmitti occurs in the Western Atlantic, from the Caribbean to Rio Grande do Sul, and it is a species of great commercial importance to the region of Sepetiba Bay - RJ. This study was made to analyze the main feature secretions of the vas deferens and the sperm viability in fresh prawns and post mortem and their usefullness for reproduction. The first study aimed to compare through the histological characterization the area of the seminiferous tubules in different seasons; comparing histologically and histochemically the secretions of the vas deferens (the proximal and medial regions) and the terminal ampoule, as well as the ultrastructure of spermatozoa in the regions of the white shrimp L. schmitti fresh and post mortem, characterizing the morphology of the sperm through the transmission electron microscopy and the reproductive system through the scanning electron microscopy. The animals came from the commercial fishing of Sepetiba Bay (Saco da Pombeba) latitude 23 ? 3'13 .91 "S and longitude 43 ? 54'44 .93" W. 21 adult males were bought post mortem (approximately 48 hours), every season, spring, summer, autumn and winter (totaling 84 animals), and three living adult males (fresh) for each season. The area of the testicles appeared higher in summer indicating that this is the period of the year of full reproductive activity and lower in winter, suggesting that this is the resting period of this species in the Sepetiba Bay - RJ. Through histological and ultrastructural analysis of sperm vas deferens and terminal ampoule of animals post mortem it was found that it is possible to use these spermatophore males to inseminate females as well as for their cryopreservation. The secretions present in the medial vas deferens, terminal ampoule and lumen spermatophore possess polysaccharides and proteins, indicating that they exert protective functions of sperm and supply of energy-rich substrates, increasing their survival time (viability) in the female. The sperm of the shrimp L. schmitti is composed of the main body, the acrosomal region and the thorn source acrosomal. The second study was divided into two tests, the first was to evaluate the feasibility of two protocols of freezing sperm and spermatophore mass (using 10% Glycerol) and the second, the efficiency of two cryoprotectants (Glycerol and 10% DMSO) in both mass sperm cryopreservation, as the spermatophore using the best protocol previously evaluated. There has been used a total of 55 male adult shrimps (post mortem) for the first experiment and 65 for the second. Adult males were equaly obtained as the ones from the first study. The protocol in which the temperature fell by 0.5oC/min to reach -32oC satisfactory results for cryopreservation, however, the protocol which fell 20oC/min to reach-120oC was unsatisfactory due to low sperm survival. Both Glycerol and DMSO may be used in cryopreservation of sperm mass.
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spelling Oshiro, Lidia Miyako Yoshii987.007.668-87http://lattes.cnpq.br/8112019853480327Mello, Marco Roberto Bourg de190.517.028-92Keunecke, Karina AnnesFreitas , Simone Patricia Carneiro deYasui, George ShiguekiPeixoto, Silvio Ricardo Maurano178.776.188-67http://lattes.cnpq.br/6887362704696785Fernandes, Andrea Bambozzi2020-04-27T13:11:20Z2013-03-01FERNANDES, Andrea Bambozzi. Morfologia e criopreserva??o do espermatozoide do camar?o branco Litopenaeus schmitti (Crustacea, Decapoda, Penaeidae) 2013. 57 f. Tese (Doutorado em Zootecnia) - Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2013.https://tede.ufrrj.br/jspui/handle/jspui/3456The white shrimp Litopenaeus schmitti occurs in the Western Atlantic, from the Caribbean to Rio Grande do Sul, and it is a species of great commercial importance to the region of Sepetiba Bay - RJ. This study was made to analyze the main feature secretions of the vas deferens and the sperm viability in fresh prawns and post mortem and their usefullness for reproduction. The first study aimed to compare through the histological characterization the area of the seminiferous tubules in different seasons; comparing histologically and histochemically the secretions of the vas deferens (the proximal and medial regions) and the terminal ampoule, as well as the ultrastructure of spermatozoa in the regions of the white shrimp L. schmitti fresh and post mortem, characterizing the morphology of the sperm through the transmission electron microscopy and the reproductive system through the scanning electron microscopy. The animals came from the commercial fishing of Sepetiba Bay (Saco da Pombeba) latitude 23 ? 3'13 .91 "S and longitude 43 ? 54'44 .93" W. 21 adult males were bought post mortem (approximately 48 hours), every season, spring, summer, autumn and winter (totaling 84 animals), and three living adult males (fresh) for each season. The area of the testicles appeared higher in summer indicating that this is the period of the year of full reproductive activity and lower in winter, suggesting that this is the resting period of this species in the Sepetiba Bay - RJ. Through histological and ultrastructural analysis of sperm vas deferens and terminal ampoule of animals post mortem it was found that it is possible to use these spermatophore males to inseminate females as well as for their cryopreservation. The secretions present in the medial vas deferens, terminal ampoule and lumen spermatophore possess polysaccharides and proteins, indicating that they exert protective functions of sperm and supply of energy-rich substrates, increasing their survival time (viability) in the female. The sperm of the shrimp L. schmitti is composed of the main body, the acrosomal region and the thorn source acrosomal. The second study was divided into two tests, the first was to evaluate the feasibility of two protocols of freezing sperm and spermatophore mass (using 10% Glycerol) and the second, the efficiency of two cryoprotectants (Glycerol and 10% DMSO) in both mass sperm cryopreservation, as the spermatophore using the best protocol previously evaluated. There has been used a total of 55 male adult shrimps (post mortem) for the first experiment and 65 for the second. Adult males were equaly obtained as the ones from the first study. The protocol in which the temperature fell by 0.5oC/min to reach -32oC satisfactory results for cryopreservation, however, the protocol which fell 20oC/min to reach-120oC was unsatisfactory due to low sperm survival. Both Glycerol and DMSO may be used in cryopreservation of sperm mass.O camar?o branco Litopenaeus schmitti ocorre no Atl?ntico Ocidental, das Antilhas ao Rio Grande do Sul, e ? uma esp?cie de grande import?ncia comercial para a regi?o da Ba?a de Sepetiba - RJ. Este trabalho teve como principal caracter?stica analisar as secre??es do vaso deferente e a viabilidade dos espermatozoides em camar?es frescos e post mortem para utiliza??o dos mesmos na reprodu??o. O primeiro estudo teve por objetivos comparar atrav?s da caracteriza??o histol?gica a ?rea dos t?bulos semin?feros nas diferentes esta??es do ano; comparar histol?gica e histoquimicamente as secre??es do vaso deferente (regi?es proximal e m?dio) e da ampola terminal, assim como a ultraestrutura dos espermatozoides nestas regi?es do camar?o branco L. schmitti frescos e post mortem, caracterizar morfologicamente os espermatozoides atrav?s de microscopia eletr?nica de transmiss?o e o sistema reprodutor pela microscopia eletr?nica de varredura. Os animais foram oriundos da pesca comercial da ba?a de Sepetiba (Saco da Pombeba) latitude 23? 3'13.91"S e longitude 43?54'44.93"O. Foram comprados 21 machos adultos post mortem (aproximadamente a 48h), em cada esta??o, na primavera, ver?o, outono e inverno (totalizando 84 animais), e tr?s machos adultos vivos (frescos) para cada esta??o do ano. A ?rea do test?culo se apresentou superior no ver?o indicando ser esta a ?poca do ano de plena atividade reprodutiva e inferior no inverno, sugerindo ser este o per?odo de repouso desta esp?cie na ba?a de Sepetiba - RJ. Por meio das an?lises histol?gicas e ultraestruturais dos espermatozoides do vaso deferente e da ampola terminal de animais post mortem constatou-se, que ? poss?vel a utiliza??o dos espermat?foros destes machos para a insemina??o de f?meas, assim como para a criopreserva??o dos mesmos. As secre??es presentes no vaso deferente m?dio, na ampola terminal e no l?men dos espermat?foros possuem polissacar?deos e prote?nas, o que indica que estas exercem as fun??es de prote??o dos espermatozoides e fornecimento de substratos ricos em energia, aumentando o tempo de sobreviv?ncia (viabilidade) dos mesmos na f?mea. O espermatozoide do camar?o L. schmitti ? composto por um corpo principal, a regi?o across?mica e o espinho de origem acrossomal. O segundo estudo foi dividido em dois ensaios, o primeiro teve por objetivo avaliar a viabilidade de dois protocolos de congelamento da massa esperm?tica e do espermat?foro (utilizando Glicerol a 10%) e o segundo a efici?ncia de dois crioprotetores (Glicerol e DMSO a 10%) na criopreserva??o tanto da massa esperm?tica, quanto do espermat?foro utilizando o melhor protocolo avaliado anteriormente. Foram utilizados um total de 55 camar?es machos adultos (post mortem) para o primeiro experimento e 65 para o segundo. Os machos adultos foram adquiridos de maneira semelhante ao do primeiro estudo. O protocolo em que a temperatura apresentou queda de 0,5oC/min at? atingir -32oC apresentou resultados satisfat?rios para a criopreserva??o, entretanto, o protocolo que apresentou queda de 20oC/min at? alcan?ar -120oC foi insatisfat?rio devido a baixa sobreviv?ncia esperm?tica. Tanto o Glicerol como o DMSO pode ser utilizado na criopreserva??o da massa esperm?tica.Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2020-04-27T13:11:20Z No. of bitstreams: 1 2013 - Andrea Bambozzi Fernandes.pdf: 2475003 bytes, checksum: f8af18d5dd441383198458537a9d7981 (MD5)Made available in DSpace on 2020-04-27T13:11:20Z (GMT). No. of bitstreams: 1 2013 - Andrea Bambozzi Fernandes.pdf: 2475003 bytes, checksum: f8af18d5dd441383198458537a9d7981 (MD5) Previous issue date: 2013-03-01REUNI - Restaura??o e Expans?o das Universidades Federaisapplication/pdfhttps://tede.ufrrj.br/retrieve/14378/2013%20-%20Andrea%20Bambozzi%20Fernandes.pdf.jpghttps://tede.ufrrj.br/retrieve/17432/2013%20-%20Andrea%20Bambozzi%20Fernandes.pdf.jpghttps://tede.ufrrj.br/retrieve/23798/2013%20-%20Andrea%20Bambozzi%20Fernandes.pdf.jpghttps://tede.ufrrj.br/retrieve/30194/2013%20-%20Andrea%20Bambozzi%20Fernandes.pdf.jpghttps://tede.ufrrj.br/retrieve/36542/2013%20-%20Andrea%20Bambozzi%20Fernandes.pdf.jpghttps://tede.ufrrj.br/retrieve/42882/2013%20-%20Andrea%20Bambozzi%20Fernandes.pdf.jpghttps://tede.ufrrj.br/retrieve/49254/2013%20-%20Andrea%20Bambozzi%20Fernandes.pdf.jpghttps://tede.ufrrj.br/retrieve/55800/2013%20-%20Andrea%20Bambozzi%20Fernandes.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em ZootecniaUFRRJBrasilInstituto de ZootecniaABRAHAM, J.; MNISSERI, M.K.; SANIL, N.K. Internal anatomy of the ampoule of Metapenaeus monoceros (Fabricius, 1798) and its role in spermatophore formation. Indian Journal Fishery, v. 59, n. 2, p. 109-113, 2012. ALFARO, J. Ultraestructura de la gl?ndula androg?nica, espermatog?nesis y oog?nesis de camarones marinos (Decapoda: Penaeidae). Revista de Biolog?a Tropical, 42, 121?129, 1994. ALFARO, J.M.; ULATE, K.; VARGAS, M. Sperm maturation and capacitation in the open thelycum shrimp Litopenaeus (Crustacea: Decapoda: Penaeoidea). Aquaculture, v. 270, p. 436-442, 2007. ALFARO, J.M; MU?OZ, N.; VARGAS, M.; KOMEN, J. Induction of sperm activation in open and closed thelycum penaeoid shrimps. Aquaculture, v.216, p. 371-381, 2003. AUNGSUCHAWAN, S.; BROWDY, C.L.; WITHYACHUMNARNKUL, B. Sperm capacitation of the shrimp Litopenaeus vannamei. Aquaculture Research, v.42, P. 188-195, 2011. BAUER, RT. 1991. Sperm transfer and storage structures in penaeoid shrimp: a functional and phylogenetic perspective. In: BAUER, RT e JW MARTIN (eds.). Crustacean Sexual Biology. Columbia University Press, USA, Chap 11: 183-207. BAUER, R.T. e CASH, C.E. Spermatophore structure and anatomy of the ejaculatory duct in Penaeus setiferus, P. duorarum and P. aztecus (Crustacea: Decapoda): homologies and functional significance. Transactions of the American Microscopical Society, 110, v.2, 144-162. 1991. BAUER, R.T. e MIN, L.J. Spermatophores and Plug Substance of the Marine Shrimp Trachypenaeus similis (Crustacea: Decapoda: Penaeidae): Formation in the Male Reproductive Tract and Disposition in the Inseminated Female. Biological Bulletin, 185, p. 174-185, 1993. BELL, T.A. e LIGHTNER, D.V. Manual de histolog?a del camar?n penaeido normal. World Aquaculture Society. Aquaculture Development Program, State of Hawaii, 114p, 1988. BRAGA, A.L.S. Ultraestrutura do espermatozoide e efeito da alimenta??o na qualidade esperm?tica do camar?o rosa Farfantepenaeus paulensis (Crustacea, Decapoda). Disserta??o (Mestrado em Oceanografia). Universidade Federal do Rio Grande, 2009. 83p. BUENO, S.L.S. Maturation and spawning of the white shrimp Penaeus schmitti Burkenroad, 1936, under large scale rearing conditions. World Aquaculture Society, v. 21, n. 3, p.170-179, 1990. BUGNOT, A.B. e L?PEZ-GRECO, L. Sperm production in the red claw crayfish Cherax quadricarinatus (Decapoda, Parastacidae). Aquaculture, v. 295, p. 292-299, 2009. 34 CAMPOS, B.R.; DUMONT, L.F.C.; D?INCAO, F.; BRANCO, J.O.J. Ovarian development and length at first maturity of the sea?bob?shrimp Xiphopenaeus kroyeri (Heller) based on histological analysis. Nauplius, v. 17, n. 1, p. 9-12, 2009. CASTIGLIONI, D.S.; FRANSOZO, M.L.N.; L?PEZ-GRECO, L.S.; SILVEIRA, A.F.; SILVEIRA, S.O. Gonad development in females of Uca rapax (Smith, 1870) (Crustacea, Brachyura, Ocypodidae) using macro and microscopic techniques. Iheringia. S?rie Zoologia, v. 97, p. 505-510, 2007. CHOW, S.; DOUGHERTY, M.M.; DOUGHERTY, W.J.; SANDIFER, P.A. Spermatophore formation in the white shrimps Penaeus setiferus and P. vannamei. Journal of Crustacean Biology, v.11, n.2, p. 201-216, 1991. CLARK JR., W.H., YUDIN, A.I., GRIFFIN, F.J., SHIGEKAWA, K. 1984. The control of gamete activation and fertilization in the marine Penaeidae, Sicyonia ingentis. In: ENGELS, W., CLARK JR., W.H., FISHER, A., OLIVE, P.G.W., WENT, D.F. (Eds.), Advances in Invertebrate Reproduction, vol. 3. Elsevier Science Publisher, Amsterdam, The Netherlands, pp. 459?472. COELHO, P.A. e SANTOS, M.C.F. ?poca da reprodu??o do camar?o branco, Penaeus schmitti Burkenroad (Crustacea, Decapoda, Penaeidae, na regi?o de Tamandar?, PE. Boletim T?cnico Cient?fico CEPENE, Rio Formoso, v. 1, p. 157-171, 1993. COELHO, P.A. e SANTOS, M.C.F. ?poca da reprodu??o dos camar?es Penaeus schmitti Burkenroad, 1936 e Penaeus subtilis P?rez-farfante, 1967 (Crustacea, Decapoda, Penaeidae), na regi?o da Foz do Rio S?o Francisco. Boletim T?cnico Cient?fico CEPENE, Tamandar?, v.3, n.1, p.122 ? 140, 1995. DALL, W.; HILL, B.J.; ROTHLISBERG, O.C.; STAPLES, D.J. 1990. The biology of the Penaeidae. In: BLAXTER, J.H. e SOUTHWARD, A.J. (eds.). Advances in Marine Biology. Academic Press, UK, vol. 27, 489p., 1990. DIAZ, A.C.; GIMENEZ, F.A.V.;PETREL, A.M.; FENUCCI, J.L. Morphological and functional study of the male reproductive tract in the shrimp Pleoticus muelleri Bate (Decapoda, Penaeoide). Invertebrate Reproduction and Development. v. 42, n. 1, p. 69-74, 2002. DOUGHERTY, W.J. Oriented spermatozoa in the spermatophore of the palaemonid shrimp, Macrobrachium rosenbergii. Tissue e Cell, v.19, p. 145-152, 1987. FELGENHAUER, B.E. e ABELE, L.G. 1991. Morphological diversity of decapod spermatozoa. In: Bauer, R. T. and Martin, J. W. (Eds): Crustacean Sexual Biology, pp. 322?341. Columbia University Press, New York, NY, USA. FRANSOZO, V.N. Morfologia dos caracteres sexuais secund?rios e caracteriza??o gonadalmasculina em Xiphopenaeus kroyeri (HELLER, 1862) (Crustacea, Dendrobranchiata, Penaeoidea). Disserta??o (Mestrado em Ci?ncias Biol?gicas). Universidade Estadual Paulista, 2008. 73p. JEYALECTUMIE, C. e SUBRAMONIAM, T. Cryopreservation of spermatophores and seminal plasma of the edible crab Scylla serrata. Biological Bulletin, v. 177, p. 247-253, 1989. 35 KAO, H.C.; CHAN, T.Y.; YU, H.P. Ovary Development of the Deep-water Shrimp Aristaeomorpha foliacea (Risso, 1826) (Crustacea: Decapoda: Aristeidae) from Taiwan Zoological Studies, v.38, n.4, p. 373-378, 1999. KARNOVSKY, M.J. A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. Journal Cell Biology, v.27, 137A, 1965. KING, J.E. A study of the reproductive organs of the common marine shrimp, Penaeus setiferus (Linnaeus). Biological Bulletin, v.94, p. 244-262, 1948. KLEVE, M.G.; YUDIN, A.I.; CLARK JR, W.H. Fine structure of the unistellate sperm the shrimp, Sicyonia ingentis (Natantia). Tissue e Cell, v.12, n.1, p. 29-45, 1980. LEUNG-TRUJILLO, JR e LAWRENCE, A.L. Observations on the decline in sperm quality of Penaeus setiferus under laboratory conditions. Aquaculture, v. 65, p. 363? 370. 1980. MACHADO, I.F.; DUMONT, L.F.C.; D?INCAO, F. Stages of gonadal development and mean length at first maturity of wild females of white shrimp (Litopenaeus schmitti ? Decapoda, Penaeidae) in Southern Brazil. Atl?ntica, Rio Grande, v. 31, n.2, p. 169-175, 2009. MALEK, S.R.A. e BAWAB, F.M. The formation of the spermatophore in Penaeus kerathurus (FORSKAL, 1775) (DECAPODA, PENAEIDAE): I The initial formation of a sperm mass. Crustaceana, v. 26, n.3, p. 273-285, 1974a. MALEK, S.R.A. e BAWAB, F.M. The formation of the spermatophore in Penaeus kerathurus (FORSKAL, 1775) (DECAPODA, PENAEIDAE): II The deposition of the main layers of the body and of the wing. Crustaceana, v. 27, n.1, p. 73-83, 1974b. MEDINA, A.; VILA, Y.; MOURENTE, G.; RODR?GUEZ, A. A comparative study of the ovarian development in wild and pound-read shrimp, Penaeus kerathurus (Forskal, 1775). Aquaculture, v. 148, p. 63-75, 1994. MELLO, M. L. S.; VIDAL, B. C. Pr?ticas de Biologia Celular. FUNCAMP. Editora Edgard Bl?cher LTDA, Campinas-SP, 71p., 1980. MOSSOLIN, E.C. e BUENO, S.L.S. Reproductive biology of Macrobrachium olfersi (Deacapoda, Palaemonidae) in S?o Sebasti?o, Brazil. Journal of Crustacean Biology, v.22, n. 2, p. 367-376, 2002. NEIVA, G.S.; SANTOS, E.P.; JANKUSKIS, V. An?lise preliminar da popula??o de camar?o leg?timo Penaeus schmitti Burkenroa, 1936, na Ba?a de Santos - Brasil. Boletim do Instituto de Pesca, v. 1, n. 2, p. 7-14, 1971. NICOLAU, C.F.; NASCIMENTO, A.A.; MACHADO-SANTOS, C.; SALES, A.; OSHIRO, L.M.Y. Gonads of males and females of the mangrove tree crab Aratus pisonii (Grapsidae: Brachyura: Decapoda): a histological and histochemical view. Acta Zoologica (Stockholm), v. 93, n. 2, p. 222?230, 2012. PEIXOTO, S.; CAVALLI, R.O.; D?INCAO, F.; MILACH, A.O.E.M.; WASIELESKY, W. Ovarian maturation of wild Farfantepenaeus paulensis in relation to histological and visual changes. Aquaculture Research, v. 34, p. 1255-1260, 2003. 36 PEIXOTO, S.; CAVALLI, R.O.; D?INCAO, F.; WASIELESKY, W.; MILACH, A.O.E.M. A comparative study of the ovarian histology of eyestalk ablated and unablated Farfantepenaeus paulensis after spawning. Boletim do Instituto de Pesca, S?o Paulo, v. 28, n. 1, p. 71-76, 2002. PEIXOTO, S.; COMAN, G.; ARNOLD, S.; CROCOS, P.; PRESTON, N. Histological examination of final oocyte maturation and atresia in wild and domesticated Penaeus monodon (Fabricius) broodstock. Aquaculture Research, v.36, p. 666-673, 2005. P?REZ-JAR, L. Fisiolog?a y calidad reproductiva de machos de camar?n blanco Litopenaeus schmitti en condiciones de cautiverio. Tesis (Doctorado en Ciencias). Centro de investigaciones biol?gicas del Noroeste, La Paz, 2005. 150p. POLJAROEN, J.; VANICHVIRIYAKIT, R.; TINIKUL, Y.; PHOUNGPETCHARA, I.; LINTHONG, V.; WEERACHATYANUKUL, W.; SOBHON, P. Spermatogenesis and distinctive mature sperm in the giant freshwater prawn, Macrobrachium rosenbergii (De Man, 1879). Zoologischer Anzeiger, v. 249, p. 81?94, 2010. PONGTIPPATEE, P; VANICHVIRIYAKIT, R; CHAVADEJ, J; PLODPAI, P; PRATOOMCHART, B; SOBHON, P; WITHYACHUMNARNKUL, B. Acrosome reaction in the sperm of the black tiger shrimp Penaeus monodon (Decapoda, Penaeidae). Aquaculture Research, v. 38, p. 1635-1644, 2007. RO, S.; TALBOT, P.; LEUNG-TRUJILLO, J.; LAWRENCE, A.L. Structure and function of the vas deferens in the shrimp Penaeus setiferus: Segments 1-3. Journal of Crustacean Biology, v. 10, n. 3, p. 455-468, 1990. SANTOS, C.M.; LIMA, G.V.; NASCIMENTO, A.A.; SALES, A.; OSHIRO, L.M.Y. Histological and histochemical analysis of the gonadal development of males and females of Armases rubripes (Rathbun 1897) (Crustacea, Brachyura, Sesarmidae). Brazilian Journal of Biology, v. 69, p. 161-169, 2009. SAS, Statistical Analysis Software. User?s Guide, 4ed Cary,NC: SAS Inst.Inc , v.2, 1990.100p. SHIGEKAWA, K. e CLARK JR, W.H. Spermiogenesis in the Marine Shrimp, Sicyonia ingentis. Development Growth and Differentiation, v. 28, n.2, p. 95-112, 1986. SILVA, O. Alguns pene?deos e palinur?deos do Atl?ntico Sul. Rio de Janeiro: SUDEPE, 1965, 20p. SUBRAMONIAM, T. 1991. Chemical composition of spermatophores in decapod crustaceans. In: BAUER, RT e JW MARTIN (eds.). Crustacean Sexual Biology. Columbia University Press, USA. Chap 17: 308-321. SUBRAMONIAM, T. Light and electron microscopic studies on the seminal secretions and the vas deferens of the penaeiodean shrimp, Sicyonia ingentis. Journal of Bioscience, v. 20, n. 5, p 691-706, 1995. 37 ZARA, F.J.; TOYAMA, M.H.; CAETANO, F.H.; L?PEZ-GRECO, L.S. Spermatogenesis, spermatophore, and seminal fluid production in the adult blue crab Callinectes danae (Portunidae). Journal of Crustacean Biology, v. 32, n. 2, p. 249-262, 2012. WANG, Q.; MISAMORE, M.; JIANG, C.Q.; BROWDY, C.L. Egg water induced reaction and biostain assay of sperm from marine shrimp Penaeus vannamei: dietary effects on sperm quality. Journal of the World Aquaculture Society, v. 26, n. 3, p. 261-271, 1995. 1995. AKARASANON K, DAMRONGPHOL P, POOLSANGUAN W. Long-term cryopreservation of spermatophore of the giant freshwater prawn, Macrobrachium rosenbergii (de Man). Aquaculture Research, v. 35, p. 1415?1420, 2004. ALMEIDA, J.L. Efeito de diferentes concentra??es de plasma seminal na criopreserva??o do s?men eq?ino. Disserta??o (Mestrado em Ci?ncias Agr?rias). Universidade de Bras?lia. 2006. 90p. ANCHORDOGUY T.; CROWE J.H.; GRIFFIN F.J; CLARK, W.H. Cryopreservation of sperm from the marine shrimp Sicyonia ingentis. Cryobiology, v.25, p. 238?43, 1988. ARCE, S.M.; MOSS, S.M.; ARGUE, B.J. Artificial insemination and spawning of pacific white shrimp Litopenaeus vannamei: implications for a selective breeding program. UJNR Technical Report, n. 28, p. 5-8, 1999. BART, A.N.; CHOOSUK, S.; THAKUR, D.P. Spermatophore cryopreservation and artificial insemination of black tiger shrimp, Penaeus monodon (Fabricius). Aquaculture Research, v. 37, p. 523-528, 2006. BEHLMER, S.D.; BROWN, G. Viability of cryopreserved spermatozoa of the horseshoe crab, Limulus polyphemus L. International Journal Invertebrate Reproduction Development, v.7, p. 193-199, 1984. BRAVANISHANKAR, S. e SUBRAMONIAM, T. Cryopreservation of spermatozoa of the edible mud crab Scylla serrata (Forskal). Journal of Experimental Zoology, v. 277, p. 326-336, 1997. CASTELO-BRANCO, T. Avalia??o de t?cnicas de criopreserva??o de s?men do camar?o branco, Litopenaeus schmitti (Crustacea, Dendrobranchiata, Penaeidae). Disserta??o (Mestrado em Ci?ncias). Universidade Federal Rural do Rio de Janeiro, Serop?dica. 2010. 42p. CHOW, S.; TAKI, Y.; OGASAWARA, Y. Cryopreservation of spermatophore of the fresh water shrimp, Macrobrachium rosenbergii. The Biological Bulletin, v.168, p. 471?5, 1985. ELMOAZZEN, H. Y. Parameters affecting water permeability across biological cell membranes. 2000. Dissertation (Master of Science). University of Alberta, Edmonton. 141p. FABBRI, R. Cryopreservation of human oocytes and ovarian tissue. Cell and Tissue Banking, v. 7, p. 113-122, 2006. FRIEDLER, S.; GIUDICE, S. C.; LAMB, E. J. Cryopreservation of embryos and ova. Fertility and Sterility, v. 49, p. 743-764, 1988. 56 GON?ALVES, P.B.D; FIGUEIREDO, J.R.; FREITAS, V.J.F. Biot?cnicas aplicadas ? reprodu??o animal. 2a Ed. Roca, 2008, 395p. GWO, J.C. Cryopresevation of aquatic invertebrate semen: a review. Aquaculture Research, n.31, p.259-271, 2000. HOVATTA, O. Methods for cryopreservation of human ovarian tissue. Reproductive Bio Medicine Online, v.10, p. 729-734, 2005. JEYALECTUMIE, C. e SUBRAMONIAM, T. Cryopreservation of spermatophores and seminal plasma of the edible crab Scylla serrata. Biological Bulletin, v.177, p. 247-253, 1989. KARLSSON, J. O. M.; CRAVALHO, E. G.; TONER, M. Intracellular ice formation: causes and consequences. CryoLetters, v. 14, p. 323-336, 1993. LEENA GRACE, B. e NATARAJAN, P. Cryopreservation of Penaeus monodon (Fabricius, 1798) spermatozoa. Indian Journal of Fisheries, v. 50, n.3, p. 415-419, 2003. LEIBO, S. P. Sources of variation in cryopreservation. In: TIERSCH, T. R.; MAZIK, P. M. Cryopreservation in aquatic species, Baton Rouge: World Aquaculture Society, 2000, p.75-83. LEZCANO, M; GRANJA, C.; SALAZAR, M. The use of flowcytometry in the evaluation of cell viability of cryopreserved sperm of the marine shrimp (Litopenaeus vannamei). Cryobiology, v. 48, p. 349?56, 2004. MAZUR, P.; RALL, W. F.; LEIBO, S. P. Kinetics of water loss and the likelihood of intracellular freezing in mouse ova. Influence of the method of calculating the temperature dependence of water permeability. Cell Biophysics, v. 6, p. 197-213, 1984. MEMON, A.J.; TALPUR, A.D.; KHAN, M.I.; FARIDDUDIN, M.O.; SAFIAH, J. Optimization of spermatophores cryopreservation protocolo of banana shrimp (Penaeus merguiensis) (De Man, 1888). Journal of Animal and Veterinary Advances, v.11, n.10, p.1688-1704, 2012. MIES FILHO, A. Reprodu??o dos animais e insemina??o artificial. Ed. Sulina. v.2, 1982, 783p. MONTOYA, J.M. Controlled reproduction of penaeid shrimp: a contribution to its improvment. 2001,149p. MORRIS, G.I. Cryopreservation: An introduction to cryopreservation in culture collections. Ed. Institute of Terrestrial Ecology, Cambridge, 1981, 27p. NIMRAT, S.; SIRIBOONLAMOM, S.; ZHANG, S.; XU, Y.; VUTHIPHANDCHAI, V. Chilled storage of white shrimp (L. vannamei) spermatophores. Aquaculture, n. 261, p.944?951, 2006. 57 PARKS, J.E. e GRAHAM, J.K. Effects of cryopreservation procedures on sperm membranes. Theriogenology, v.38, p.209-222, 1992. P?REZ-JAR, L. Fisiolog?a y calidad reproductiva de machos de camar?n blanco Litopenaeus schmitti en condiciones de cautiverio. 2005. 150p. Tesis (Doctorado en Ciencias). Centro de investigaciones biol?gicas del Noroeste, La Paz. RESENDE, E. K. de.; MARQUES, D.K.S. Criopreserva??o de s?men de peixe. Corumb?: Embrapa Pantanal, 2009. 5 p. (Embrapa Pantanal. Circular T?cnica, 84). Dispon?vel em: <http://www.cpap.embrapa.br/publicacoes/download.php?arq_pdf=CT84>. Acesso em: 02 fev 2012. SALAZAR, M.; LEZCANO, M.; GRANJA, C. 2008. Protocol for cryopreservation of Penaeus vannamei sperm cells. In: CABRITA, E.; ROBLES, V.; HERR?EZ, P. Methods in Reproductive Aquaculture. Marine and Freshwater Species. Boca raton, FL, CRC Press, p.505-508. SHAW, J. M. In: A. Trounson, D. Gardner. In Vitro Fertilization. Florida: CRC Press, Boca Raton, 1993. Chap.11. SHAW, J.M.; WOOD, E.C.; TROUNSON, A.O. Cryopreservation of oocyte and embryo. In: TROUNSON A. e GARDNER D. Handbook of In vitro fertilization. 2a ed. Florida: CRC Press, p.376-380, 2000. UNIVERSIDADE FEDERAL DE VICOSA - UFV. SAEG: Sistema de an?lises estat?sticas e gen?ticas. Vers?o 9.1. Vi?osa, MG: 2007. 150p. VUTHIPHANDCHAI, V.; NIMRAT, S.; KOTCHARAT S.; BART, A.N. Development of a cryopreservation protocol for long-term storage of black tiger shrimp (Penaeus monodon) spermatophores. Theriogenology, v.68, p. 1192?1199, 2007. WANG, Q.Y.; MISAMORE, M.; JIANG, C.Q.; BROWDY, C.L. Egg water induced reaction and biostain assay of sperm from marine shrimp Penaeus vannamei: dietary effects on sperm quality. Journal of the World Aquaculture Society, v. 26, p. 261-271, 1995. YOO, B.Y.; RYAN, M.A.; WIGGS, A.J. Loss of protein from spermatozoa of Atlantic salmon (Salmo salar L.) because of cryopreservation. 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dc.title.por.fl_str_mv Morfologia e criopreserva??o do espermatozoide do camar?o branco Litopenaeus schmitti (Crustacea, Decapoda, Penaeidae)
dc.title.alternative.eng.fl_str_mv Morphology and cryopreservation of sperm of white shrimp Litopenaeus schmitti (Crustacea, Decapoda, Penaeidae)
title Morfologia e criopreserva??o do espermatozoide do camar?o branco Litopenaeus schmitti (Crustacea, Decapoda, Penaeidae)
spellingShingle Morfologia e criopreserva??o do espermatozoide do camar?o branco Litopenaeus schmitti (Crustacea, Decapoda, Penaeidae)
Fernandes, Andrea Bambozzi
Penaeidae
sistema reprodutor masculino
histologia
ultraestrutura
sazonalidade
Penaeidae
male reproductive system
histology
ultrastructure
Zootecnia
title_short Morfologia e criopreserva??o do espermatozoide do camar?o branco Litopenaeus schmitti (Crustacea, Decapoda, Penaeidae)
title_full Morfologia e criopreserva??o do espermatozoide do camar?o branco Litopenaeus schmitti (Crustacea, Decapoda, Penaeidae)
title_fullStr Morfologia e criopreserva??o do espermatozoide do camar?o branco Litopenaeus schmitti (Crustacea, Decapoda, Penaeidae)
title_full_unstemmed Morfologia e criopreserva??o do espermatozoide do camar?o branco Litopenaeus schmitti (Crustacea, Decapoda, Penaeidae)
title_sort Morfologia e criopreserva??o do espermatozoide do camar?o branco Litopenaeus schmitti (Crustacea, Decapoda, Penaeidae)
author Fernandes, Andrea Bambozzi
author_facet Fernandes, Andrea Bambozzi
author_role author
dc.contributor.advisor1.fl_str_mv Oshiro, Lidia Miyako Yoshii
dc.contributor.advisor1ID.fl_str_mv 987.007.668-87
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/8112019853480327
dc.contributor.advisor-co1.fl_str_mv Mello, Marco Roberto Bourg de
dc.contributor.advisor-co1ID.fl_str_mv 190.517.028-92
dc.contributor.referee1.fl_str_mv Keunecke, Karina Annes
dc.contributor.referee2.fl_str_mv Freitas , Simone Patricia Carneiro de
dc.contributor.referee3.fl_str_mv Yasui, George Shigueki
dc.contributor.referee4.fl_str_mv Peixoto, Silvio Ricardo Maurano
dc.contributor.authorID.fl_str_mv 178.776.188-67
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/6887362704696785
dc.contributor.author.fl_str_mv Fernandes, Andrea Bambozzi
contributor_str_mv Oshiro, Lidia Miyako Yoshii
Mello, Marco Roberto Bourg de
Keunecke, Karina Annes
Freitas , Simone Patricia Carneiro de
Yasui, George Shigueki
Peixoto, Silvio Ricardo Maurano
dc.subject.por.fl_str_mv Penaeidae
sistema reprodutor masculino
histologia
ultraestrutura
sazonalidade
topic Penaeidae
sistema reprodutor masculino
histologia
ultraestrutura
sazonalidade
Penaeidae
male reproductive system
histology
ultrastructure
Zootecnia
dc.subject.eng.fl_str_mv Penaeidae
male reproductive system
histology
ultrastructure
dc.subject.cnpq.fl_str_mv Zootecnia
description The white shrimp Litopenaeus schmitti occurs in the Western Atlantic, from the Caribbean to Rio Grande do Sul, and it is a species of great commercial importance to the region of Sepetiba Bay - RJ. This study was made to analyze the main feature secretions of the vas deferens and the sperm viability in fresh prawns and post mortem and their usefullness for reproduction. The first study aimed to compare through the histological characterization the area of the seminiferous tubules in different seasons; comparing histologically and histochemically the secretions of the vas deferens (the proximal and medial regions) and the terminal ampoule, as well as the ultrastructure of spermatozoa in the regions of the white shrimp L. schmitti fresh and post mortem, characterizing the morphology of the sperm through the transmission electron microscopy and the reproductive system through the scanning electron microscopy. The animals came from the commercial fishing of Sepetiba Bay (Saco da Pombeba) latitude 23 ? 3'13 .91 "S and longitude 43 ? 54'44 .93" W. 21 adult males were bought post mortem (approximately 48 hours), every season, spring, summer, autumn and winter (totaling 84 animals), and three living adult males (fresh) for each season. The area of the testicles appeared higher in summer indicating that this is the period of the year of full reproductive activity and lower in winter, suggesting that this is the resting period of this species in the Sepetiba Bay - RJ. Through histological and ultrastructural analysis of sperm vas deferens and terminal ampoule of animals post mortem it was found that it is possible to use these spermatophore males to inseminate females as well as for their cryopreservation. The secretions present in the medial vas deferens, terminal ampoule and lumen spermatophore possess polysaccharides and proteins, indicating that they exert protective functions of sperm and supply of energy-rich substrates, increasing their survival time (viability) in the female. The sperm of the shrimp L. schmitti is composed of the main body, the acrosomal region and the thorn source acrosomal. The second study was divided into two tests, the first was to evaluate the feasibility of two protocols of freezing sperm and spermatophore mass (using 10% Glycerol) and the second, the efficiency of two cryoprotectants (Glycerol and 10% DMSO) in both mass sperm cryopreservation, as the spermatophore using the best protocol previously evaluated. There has been used a total of 55 male adult shrimps (post mortem) for the first experiment and 65 for the second. Adult males were equaly obtained as the ones from the first study. The protocol in which the temperature fell by 0.5oC/min to reach -32oC satisfactory results for cryopreservation, however, the protocol which fell 20oC/min to reach-120oC was unsatisfactory due to low sperm survival. Both Glycerol and DMSO may be used in cryopreservation of sperm mass.
publishDate 2013
dc.date.issued.fl_str_mv 2013-03-01
dc.date.accessioned.fl_str_mv 2020-04-27T13:11:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv FERNANDES, Andrea Bambozzi. Morfologia e criopreserva??o do espermatozoide do camar?o branco Litopenaeus schmitti (Crustacea, Decapoda, Penaeidae) 2013. 57 f. Tese (Doutorado em Zootecnia) - Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2013.
dc.identifier.uri.fl_str_mv https://tede.ufrrj.br/jspui/handle/jspui/3456
identifier_str_mv FERNANDES, Andrea Bambozzi. Morfologia e criopreserva??o do espermatozoide do camar?o branco Litopenaeus schmitti (Crustacea, Decapoda, Penaeidae) 2013. 57 f. Tese (Doutorado em Zootecnia) - Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2013.
url https://tede.ufrrj.br/jspui/handle/jspui/3456
dc.language.iso.fl_str_mv por
language por
dc.relation.references.por.fl_str_mv ABRAHAM, J.; MNISSERI, M.K.; SANIL, N.K. Internal anatomy of the ampoule of Metapenaeus monoceros (Fabricius, 1798) and its role in spermatophore formation. Indian Journal Fishery, v. 59, n. 2, p. 109-113, 2012. ALFARO, J. Ultraestructura de la gl?ndula androg?nica, espermatog?nesis y oog?nesis de camarones marinos (Decapoda: Penaeidae). Revista de Biolog?a Tropical, 42, 121?129, 1994. ALFARO, J.M.; ULATE, K.; VARGAS, M. Sperm maturation and capacitation in the open thelycum shrimp Litopenaeus (Crustacea: Decapoda: Penaeoidea). Aquaculture, v. 270, p. 436-442, 2007. ALFARO, J.M; MU?OZ, N.; VARGAS, M.; KOMEN, J. Induction of sperm activation in open and closed thelycum penaeoid shrimps. Aquaculture, v.216, p. 371-381, 2003. AUNGSUCHAWAN, S.; BROWDY, C.L.; WITHYACHUMNARNKUL, B. Sperm capacitation of the shrimp Litopenaeus vannamei. Aquaculture Research, v.42, P. 188-195, 2011. BAUER, RT. 1991. Sperm transfer and storage structures in penaeoid shrimp: a functional and phylogenetic perspective. In: BAUER, RT e JW MARTIN (eds.). Crustacean Sexual Biology. Columbia University Press, USA, Chap 11: 183-207. BAUER, R.T. e CASH, C.E. Spermatophore structure and anatomy of the ejaculatory duct in Penaeus setiferus, P. duorarum and P. aztecus (Crustacea: Decapoda): homologies and functional significance. Transactions of the American Microscopical Society, 110, v.2, 144-162. 1991. BAUER, R.T. e MIN, L.J. Spermatophores and Plug Substance of the Marine Shrimp Trachypenaeus similis (Crustacea: Decapoda: Penaeidae): Formation in the Male Reproductive Tract and Disposition in the Inseminated Female. Biological Bulletin, 185, p. 174-185, 1993. BELL, T.A. e LIGHTNER, D.V. Manual de histolog?a del camar?n penaeido normal. World Aquaculture Society. Aquaculture Development Program, State of Hawaii, 114p, 1988. BRAGA, A.L.S. Ultraestrutura do espermatozoide e efeito da alimenta??o na qualidade esperm?tica do camar?o rosa Farfantepenaeus paulensis (Crustacea, Decapoda). Disserta??o (Mestrado em Oceanografia). Universidade Federal do Rio Grande, 2009. 83p. BUENO, S.L.S. Maturation and spawning of the white shrimp Penaeus schmitti Burkenroad, 1936, under large scale rearing conditions. World Aquaculture Society, v. 21, n. 3, p.170-179, 1990. BUGNOT, A.B. e L?PEZ-GRECO, L. Sperm production in the red claw crayfish Cherax quadricarinatus (Decapoda, Parastacidae). Aquaculture, v. 295, p. 292-299, 2009. 34 CAMPOS, B.R.; DUMONT, L.F.C.; D?INCAO, F.; BRANCO, J.O.J. Ovarian development and length at first maturity of the sea?bob?shrimp Xiphopenaeus kroyeri (Heller) based on histological analysis. Nauplius, v. 17, n. 1, p. 9-12, 2009. CASTIGLIONI, D.S.; FRANSOZO, M.L.N.; L?PEZ-GRECO, L.S.; SILVEIRA, A.F.; SILVEIRA, S.O. Gonad development in females of Uca rapax (Smith, 1870) (Crustacea, Brachyura, Ocypodidae) using macro and microscopic techniques. Iheringia. S?rie Zoologia, v. 97, p. 505-510, 2007. CHOW, S.; DOUGHERTY, M.M.; DOUGHERTY, W.J.; SANDIFER, P.A. Spermatophore formation in the white shrimps Penaeus setiferus and P. vannamei. Journal of Crustacean Biology, v.11, n.2, p. 201-216, 1991. CLARK JR., W.H., YUDIN, A.I., GRIFFIN, F.J., SHIGEKAWA, K. 1984. The control of gamete activation and fertilization in the marine Penaeidae, Sicyonia ingentis. In: ENGELS, W., CLARK JR., W.H., FISHER, A., OLIVE, P.G.W., WENT, D.F. (Eds.), Advances in Invertebrate Reproduction, vol. 3. Elsevier Science Publisher, Amsterdam, The Netherlands, pp. 459?472. COELHO, P.A. e SANTOS, M.C.F. ?poca da reprodu??o do camar?o branco, Penaeus schmitti Burkenroad (Crustacea, Decapoda, Penaeidae, na regi?o de Tamandar?, PE. Boletim T?cnico Cient?fico CEPENE, Rio Formoso, v. 1, p. 157-171, 1993. COELHO, P.A. e SANTOS, M.C.F. ?poca da reprodu??o dos camar?es Penaeus schmitti Burkenroad, 1936 e Penaeus subtilis P?rez-farfante, 1967 (Crustacea, Decapoda, Penaeidae), na regi?o da Foz do Rio S?o Francisco. Boletim T?cnico Cient?fico CEPENE, Tamandar?, v.3, n.1, p.122 ? 140, 1995. DALL, W.; HILL, B.J.; ROTHLISBERG, O.C.; STAPLES, D.J. 1990. The biology of the Penaeidae. In: BLAXTER, J.H. e SOUTHWARD, A.J. (eds.). Advances in Marine Biology. Academic Press, UK, vol. 27, 489p., 1990. DIAZ, A.C.; GIMENEZ, F.A.V.;PETREL, A.M.; FENUCCI, J.L. Morphological and functional study of the male reproductive tract in the shrimp Pleoticus muelleri Bate (Decapoda, Penaeoide). Invertebrate Reproduction and Development. v. 42, n. 1, p. 69-74, 2002. DOUGHERTY, W.J. Oriented spermatozoa in the spermatophore of the palaemonid shrimp, Macrobrachium rosenbergii. Tissue e Cell, v.19, p. 145-152, 1987. FELGENHAUER, B.E. e ABELE, L.G. 1991. Morphological diversity of decapod spermatozoa. In: Bauer, R. T. and Martin, J. W. (Eds): Crustacean Sexual Biology, pp. 322?341. Columbia University Press, New York, NY, USA. FRANSOZO, V.N. Morfologia dos caracteres sexuais secund?rios e caracteriza??o gonadalmasculina em Xiphopenaeus kroyeri (HELLER, 1862) (Crustacea, Dendrobranchiata, Penaeoidea). Disserta??o (Mestrado em Ci?ncias Biol?gicas). Universidade Estadual Paulista, 2008. 73p. JEYALECTUMIE, C. e SUBRAMONIAM, T. Cryopreservation of spermatophores and seminal plasma of the edible crab Scylla serrata. Biological Bulletin, v. 177, p. 247-253, 1989. 35 KAO, H.C.; CHAN, T.Y.; YU, H.P. Ovary Development of the Deep-water Shrimp Aristaeomorpha foliacea (Risso, 1826) (Crustacea: Decapoda: Aristeidae) from Taiwan Zoological Studies, v.38, n.4, p. 373-378, 1999. KARNOVSKY, M.J. A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. Journal Cell Biology, v.27, 137A, 1965. KING, J.E. A study of the reproductive organs of the common marine shrimp, Penaeus setiferus (Linnaeus). Biological Bulletin, v.94, p. 244-262, 1948. KLEVE, M.G.; YUDIN, A.I.; CLARK JR, W.H. Fine structure of the unistellate sperm the shrimp, Sicyonia ingentis (Natantia). Tissue e Cell, v.12, n.1, p. 29-45, 1980. LEUNG-TRUJILLO, JR e LAWRENCE, A.L. Observations on the decline in sperm quality of Penaeus setiferus under laboratory conditions. Aquaculture, v. 65, p. 363? 370. 1980. MACHADO, I.F.; DUMONT, L.F.C.; D?INCAO, F. Stages of gonadal development and mean length at first maturity of wild females of white shrimp (Litopenaeus schmitti ? Decapoda, Penaeidae) in Southern Brazil. Atl?ntica, Rio Grande, v. 31, n.2, p. 169-175, 2009. MALEK, S.R.A. e BAWAB, F.M. The formation of the spermatophore in Penaeus kerathurus (FORSKAL, 1775) (DECAPODA, PENAEIDAE): I The initial formation of a sperm mass. Crustaceana, v. 26, n.3, p. 273-285, 1974a. MALEK, S.R.A. e BAWAB, F.M. The formation of the spermatophore in Penaeus kerathurus (FORSKAL, 1775) (DECAPODA, PENAEIDAE): II The deposition of the main layers of the body and of the wing. Crustaceana, v. 27, n.1, p. 73-83, 1974b. MEDINA, A.; VILA, Y.; MOURENTE, G.; RODR?GUEZ, A. A comparative study of the ovarian development in wild and pound-read shrimp, Penaeus kerathurus (Forskal, 1775). Aquaculture, v. 148, p. 63-75, 1994. MELLO, M. L. S.; VIDAL, B. C. Pr?ticas de Biologia Celular. FUNCAMP. Editora Edgard Bl?cher LTDA, Campinas-SP, 71p., 1980. MOSSOLIN, E.C. e BUENO, S.L.S. Reproductive biology of Macrobrachium olfersi (Deacapoda, Palaemonidae) in S?o Sebasti?o, Brazil. Journal of Crustacean Biology, v.22, n. 2, p. 367-376, 2002. NEIVA, G.S.; SANTOS, E.P.; JANKUSKIS, V. An?lise preliminar da popula??o de camar?o leg?timo Penaeus schmitti Burkenroa, 1936, na Ba?a de Santos - Brasil. Boletim do Instituto de Pesca, v. 1, n. 2, p. 7-14, 1971. NICOLAU, C.F.; NASCIMENTO, A.A.; MACHADO-SANTOS, C.; SALES, A.; OSHIRO, L.M.Y. Gonads of males and females of the mangrove tree crab Aratus pisonii (Grapsidae: Brachyura: Decapoda): a histological and histochemical view. Acta Zoologica (Stockholm), v. 93, n. 2, p. 222?230, 2012. PEIXOTO, S.; CAVALLI, R.O.; D?INCAO, F.; MILACH, A.O.E.M.; WASIELESKY, W. Ovarian maturation of wild Farfantepenaeus paulensis in relation to histological and visual changes. Aquaculture Research, v. 34, p. 1255-1260, 2003. 36 PEIXOTO, S.; CAVALLI, R.O.; D?INCAO, F.; WASIELESKY, W.; MILACH, A.O.E.M. A comparative study of the ovarian histology of eyestalk ablated and unablated Farfantepenaeus paulensis after spawning. Boletim do Instituto de Pesca, S?o Paulo, v. 28, n. 1, p. 71-76, 2002. PEIXOTO, S.; COMAN, G.; ARNOLD, S.; CROCOS, P.; PRESTON, N. Histological examination of final oocyte maturation and atresia in wild and domesticated Penaeus monodon (Fabricius) broodstock. Aquaculture Research, v.36, p. 666-673, 2005. P?REZ-JAR, L. Fisiolog?a y calidad reproductiva de machos de camar?n blanco Litopenaeus schmitti en condiciones de cautiverio. Tesis (Doctorado en Ciencias). Centro de investigaciones biol?gicas del Noroeste, La Paz, 2005. 150p. POLJAROEN, J.; VANICHVIRIYAKIT, R.; TINIKUL, Y.; PHOUNGPETCHARA, I.; LINTHONG, V.; WEERACHATYANUKUL, W.; SOBHON, P. Spermatogenesis and distinctive mature sperm in the giant freshwater prawn, Macrobrachium rosenbergii (De Man, 1879). Zoologischer Anzeiger, v. 249, p. 81?94, 2010. PONGTIPPATEE, P; VANICHVIRIYAKIT, R; CHAVADEJ, J; PLODPAI, P; PRATOOMCHART, B; SOBHON, P; WITHYACHUMNARNKUL, B. Acrosome reaction in the sperm of the black tiger shrimp Penaeus monodon (Decapoda, Penaeidae). Aquaculture Research, v. 38, p. 1635-1644, 2007. RO, S.; TALBOT, P.; LEUNG-TRUJILLO, J.; LAWRENCE, A.L. Structure and function of the vas deferens in the shrimp Penaeus setiferus: Segments 1-3. Journal of Crustacean Biology, v. 10, n. 3, p. 455-468, 1990. SANTOS, C.M.; LIMA, G.V.; NASCIMENTO, A.A.; SALES, A.; OSHIRO, L.M.Y. Histological and histochemical analysis of the gonadal development of males and females of Armases rubripes (Rathbun 1897) (Crustacea, Brachyura, Sesarmidae). Brazilian Journal of Biology, v. 69, p. 161-169, 2009. SAS, Statistical Analysis Software. User?s Guide, 4ed Cary,NC: SAS Inst.Inc , v.2, 1990.100p. SHIGEKAWA, K. e CLARK JR, W.H. Spermiogenesis in the Marine Shrimp, Sicyonia ingentis. Development Growth and Differentiation, v. 28, n.2, p. 95-112, 1986. SILVA, O. Alguns pene?deos e palinur?deos do Atl?ntico Sul. Rio de Janeiro: SUDEPE, 1965, 20p. SUBRAMONIAM, T. 1991. Chemical composition of spermatophores in decapod crustaceans. In: BAUER, RT e JW MARTIN (eds.). Crustacean Sexual Biology. Columbia University Press, USA. Chap 17: 308-321. SUBRAMONIAM, T. Light and electron microscopic studies on the seminal secretions and the vas deferens of the penaeiodean shrimp, Sicyonia ingentis. Journal of Bioscience, v. 20, n. 5, p 691-706, 1995. 37 ZARA, F.J.; TOYAMA, M.H.; CAETANO, F.H.; L?PEZ-GRECO, L.S. Spermatogenesis, spermatophore, and seminal fluid production in the adult blue crab Callinectes danae (Portunidae). Journal of Crustacean Biology, v. 32, n. 2, p. 249-262, 2012. WANG, Q.; MISAMORE, M.; JIANG, C.Q.; BROWDY, C.L. Egg water induced reaction and biostain assay of sperm from marine shrimp Penaeus vannamei: dietary effects on sperm quality. Journal of the World Aquaculture Society, v. 26, n. 3, p. 261-271, 1995. 1995. AKARASANON K, DAMRONGPHOL P, POOLSANGUAN W. Long-term cryopreservation of spermatophore of the giant freshwater prawn, Macrobrachium rosenbergii (de Man). Aquaculture Research, v. 35, p. 1415?1420, 2004. ALMEIDA, J.L. Efeito de diferentes concentra??es de plasma seminal na criopreserva??o do s?men eq?ino. Disserta??o (Mestrado em Ci?ncias Agr?rias). Universidade de Bras?lia. 2006. 90p. ANCHORDOGUY T.; CROWE J.H.; GRIFFIN F.J; CLARK, W.H. Cryopreservation of sperm from the marine shrimp Sicyonia ingentis. Cryobiology, v.25, p. 238?43, 1988. ARCE, S.M.; MOSS, S.M.; ARGUE, B.J. Artificial insemination and spawning of pacific white shrimp Litopenaeus vannamei: implications for a selective breeding program. UJNR Technical Report, n. 28, p. 5-8, 1999. BART, A.N.; CHOOSUK, S.; THAKUR, D.P. Spermatophore cryopreservation and artificial insemination of black tiger shrimp, Penaeus monodon (Fabricius). Aquaculture Research, v. 37, p. 523-528, 2006. BEHLMER, S.D.; BROWN, G. Viability of cryopreserved spermatozoa of the horseshoe crab, Limulus polyphemus L. International Journal Invertebrate Reproduction Development, v.7, p. 193-199, 1984. BRAVANISHANKAR, S. e SUBRAMONIAM, T. Cryopreservation of spermatozoa of the edible mud crab Scylla serrata (Forskal). Journal of Experimental Zoology, v. 277, p. 326-336, 1997. CASTELO-BRANCO, T. Avalia??o de t?cnicas de criopreserva??o de s?men do camar?o branco, Litopenaeus schmitti (Crustacea, Dendrobranchiata, Penaeidae). Disserta??o (Mestrado em Ci?ncias). Universidade Federal Rural do Rio de Janeiro, Serop?dica. 2010. 42p. CHOW, S.; TAKI, Y.; OGASAWARA, Y. Cryopreservation of spermatophore of the fresh water shrimp, Macrobrachium rosenbergii. The Biological Bulletin, v.168, p. 471?5, 1985. ELMOAZZEN, H. Y. Parameters affecting water permeability across biological cell membranes. 2000. Dissertation (Master of Science). University of Alberta, Edmonton. 141p. FABBRI, R. Cryopreservation of human oocytes and ovarian tissue. Cell and Tissue Banking, v. 7, p. 113-122, 2006. FRIEDLER, S.; GIUDICE, S. C.; LAMB, E. J. Cryopreservation of embryos and ova. Fertility and Sterility, v. 49, p. 743-764, 1988. 56 GON?ALVES, P.B.D; FIGUEIREDO, J.R.; FREITAS, V.J.F. Biot?cnicas aplicadas ? reprodu??o animal. 2a Ed. Roca, 2008, 395p. GWO, J.C. Cryopresevation of aquatic invertebrate semen: a review. Aquaculture Research, n.31, p.259-271, 2000. HOVATTA, O. Methods for cryopreservation of human ovarian tissue. Reproductive Bio Medicine Online, v.10, p. 729-734, 2005. JEYALECTUMIE, C. e SUBRAMONIAM, T. Cryopreservation of spermatophores and seminal plasma of the edible crab Scylla serrata. Biological Bulletin, v.177, p. 247-253, 1989. KARLSSON, J. O. M.; CRAVALHO, E. G.; TONER, M. Intracellular ice formation: causes and consequences. CryoLetters, v. 14, p. 323-336, 1993. LEENA GRACE, B. e NATARAJAN, P. Cryopreservation of Penaeus monodon (Fabricius, 1798) spermatozoa. Indian Journal of Fisheries, v. 50, n.3, p. 415-419, 2003. LEIBO, S. P. Sources of variation in cryopreservation. In: TIERSCH, T. R.; MAZIK, P. M. Cryopreservation in aquatic species, Baton Rouge: World Aquaculture Society, 2000, p.75-83. LEZCANO, M; GRANJA, C.; SALAZAR, M. The use of flowcytometry in the evaluation of cell viability of cryopreserved sperm of the marine shrimp (Litopenaeus vannamei). Cryobiology, v. 48, p. 349?56, 2004. MAZUR, P.; RALL, W. F.; LEIBO, S. P. Kinetics of water loss and the likelihood of intracellular freezing in mouse ova. Influence of the method of calculating the temperature dependence of water permeability. Cell Biophysics, v. 6, p. 197-213, 1984. MEMON, A.J.; TALPUR, A.D.; KHAN, M.I.; FARIDDUDIN, M.O.; SAFIAH, J. Optimization of spermatophores cryopreservation protocolo of banana shrimp (Penaeus merguiensis) (De Man, 1888). Journal of Animal and Veterinary Advances, v.11, n.10, p.1688-1704, 2012. MIES FILHO, A. Reprodu??o dos animais e insemina??o artificial. Ed. Sulina. v.2, 1982, 783p. MONTOYA, J.M. Controlled reproduction of penaeid shrimp: a contribution to its improvment. 2001,149p. MORRIS, G.I. Cryopreservation: An introduction to cryopreservation in culture collections. Ed. Institute of Terrestrial Ecology, Cambridge, 1981, 27p. NIMRAT, S.; SIRIBOONLAMOM, S.; ZHANG, S.; XU, Y.; VUTHIPHANDCHAI, V. Chilled storage of white shrimp (L. vannamei) spermatophores. Aquaculture, n. 261, p.944?951, 2006. 57 PARKS, J.E. e GRAHAM, J.K. Effects of cryopreservation procedures on sperm membranes. Theriogenology, v.38, p.209-222, 1992. P?REZ-JAR, L. Fisiolog?a y calidad reproductiva de machos de camar?n blanco Litopenaeus schmitti en condiciones de cautiverio. 2005. 150p. Tesis (Doctorado en Ciencias). Centro de investigaciones biol?gicas del Noroeste, La Paz. RESENDE, E. K. de.; MARQUES, D.K.S. Criopreserva??o de s?men de peixe. Corumb?: Embrapa Pantanal, 2009. 5 p. (Embrapa Pantanal. Circular T?cnica, 84). Dispon?vel em: <http://www.cpap.embrapa.br/publicacoes/download.php?arq_pdf=CT84>. Acesso em: 02 fev 2012. SALAZAR, M.; LEZCANO, M.; GRANJA, C. 2008. Protocol for cryopreservation of Penaeus vannamei sperm cells. In: CABRITA, E.; ROBLES, V.; HERR?EZ, P. Methods in Reproductive Aquaculture. Marine and Freshwater Species. Boca raton, FL, CRC Press, p.505-508. SHAW, J. M. In: A. Trounson, D. Gardner. In Vitro Fertilization. Florida: CRC Press, Boca Raton, 1993. Chap.11. SHAW, J.M.; WOOD, E.C.; TROUNSON, A.O. Cryopreservation of oocyte and embryo. In: TROUNSON A. e GARDNER D. Handbook of In vitro fertilization. 2a ed. Florida: CRC Press, p.376-380, 2000. UNIVERSIDADE FEDERAL DE VICOSA - UFV. SAEG: Sistema de an?lises estat?sticas e gen?ticas. Vers?o 9.1. Vi?osa, MG: 2007. 150p. VUTHIPHANDCHAI, V.; NIMRAT, S.; KOTCHARAT S.; BART, A.N. Development of a cryopreservation protocol for long-term storage of black tiger shrimp (Penaeus monodon) spermatophores. Theriogenology, v.68, p. 1192?1199, 2007. WANG, Q.Y.; MISAMORE, M.; JIANG, C.Q.; BROWDY, C.L. Egg water induced reaction and biostain assay of sperm from marine shrimp Penaeus vannamei: dietary effects on sperm quality. Journal of the World Aquaculture Society, v. 26, p. 261-271, 1995. YOO, B.Y.; RYAN, M.A.; WIGGS, A.J. Loss of protein from spermatozoa of Atlantic salmon (Salmo salar L.) because of cryopreservation. Canadian Journal of Zoology, v.65, p. 9-13, 1987.
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