Efeitos dos extratos aquoso e hidro-alco?lico e das solu??es ultradilu?das de Palicourea marcgravii (Rubiaceae) em ratos
Ano de defesa: | 2007 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal Rural do Rio de Janeiro
|
Programa de Pós-Graduação: |
Programa de P?s-Gradua??o em Ci?ncias Veterin?rias
|
Departamento: |
Instituto de Veterin?ria
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | https://tede.ufrrj.br/jspui/handle/tede/822 |
Resumo: | Palicourea marcgravii (Pm) is the most important toxic plant in Brazil. It is responsible for about half of all bovine deaths by natural poisoning in the country. The poisoning has a hyperacute evolution resulting in sudden death, which is attributed to the monofluoroacetic acid. This substance has high toxicity to several mammals, including humans. The homeopathic therapeutics uses ultra-highly diluted and dynamized solutions and there is enough evidence to investigate them as possible protection against poisonings. The aims of the present work were to compare the toxic effects of aqueous (AE) and hydroalcoholic (mother tincture - MT) extracts of Pm in rats, evaluate the effects of ultra-highly diluted and dynamized solutions (Pm 6CH and Pm 30CH) over the development of tolerance to the toxic effects of Pm and evaluate clinical, necroscopic and histopathological alterations in nonintoxicated rats treated with these solutions. AE and MT groups were formed of 10 animals each which received the extract in doses of 0.4g/kg, 0.8g/kg, 2g/kg and 4g/kg, intragastrically. The UD6EA and UD30EA groups were formed of five rats each, which received respectively 1 mL of Pm 6CH e Pm 30CH solutions, by oral route, three times a day, for eight days. Then, they were intoxicated with 2g/kg of Pm aqueous extract, intragastrically and received the correspondent ultra-highly diluted and dynamized preparations hourly until death. The UD6 and UD30 groups were formed of five rats each and received respectively 1 mL of Pm 6CH and Pm 30CH, orally, three times a day for 63 days. The animals were evaluated using clinical parameters, including the direct observation of their behavior at the open field and at the elevated plus-maze, and the study of the macro and microscopic lesions. Hyperacute death occurred after administration of the doses of 0.4g/kg, 0.8g/kg, 2g/kg e 4g/kg. In the AE group, prostration and nervous hyperexcitability followed or not by convulsive crisis were observed. In the MT group, the animals presented marked nervous depression without convulsion. Hepatic congestion and evidence of cardiac dilation were observed in the necropsy. Hydropic vacuolar degeneration of the renal distal convoluted tubules and congestion of several organs were observed in the histopathological examination. The latencies for the emergence of the first clinical signs, the convulsions and death occurrence were different in the animals of groups AE1 (Pm 2g/kg), UD6EA and UD30EA, but was considered inadequate the animals amount. Groups UD6 and UD30 did not show any clinical, behavioral, necroscopical or histopathological differences when compared to the control group. The conclusions were that the aqueous extract causes nervous excitability and convulsions while the mother tincture causes nervous depression. There is cumulative effect of the toxic substances present in the plant, and there is not evidence that the ultra-highly diluted and dynamized preparation increases the tolerance to Pm intoxication. |
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Peixoto, Paulo Fernando de Vargas270.761.210-34http://lattes.cnpq.br/3002512195318611Fran?a, Ticiana do Nascimento027.897.197-05http://lattes.cnpq.br/3545873525091315Brito, Marilene de Farias211.517.924-20http://lattes.cnpq.br/7980824063026281417.717.377-00http://lattes.cnpq.br/2778797536334256Pinto, Luiz Figueira2016-04-28T20:16:25Z2008-06-102007-12-13PINTO, Luiz Figueira. Efeitos dos extratos aquoso e hidro-alco?lico e das solu??es ultradilu?das de Palicourea marcgravii (Rubiaceae) em ratos. 2007. 209 f. Tese (Doutorado em Ci?ncias Veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2007.https://tede.ufrrj.br/jspui/handle/tede/822Palicourea marcgravii (Pm) is the most important toxic plant in Brazil. It is responsible for about half of all bovine deaths by natural poisoning in the country. The poisoning has a hyperacute evolution resulting in sudden death, which is attributed to the monofluoroacetic acid. This substance has high toxicity to several mammals, including humans. The homeopathic therapeutics uses ultra-highly diluted and dynamized solutions and there is enough evidence to investigate them as possible protection against poisonings. The aims of the present work were to compare the toxic effects of aqueous (AE) and hydroalcoholic (mother tincture - MT) extracts of Pm in rats, evaluate the effects of ultra-highly diluted and dynamized solutions (Pm 6CH and Pm 30CH) over the development of tolerance to the toxic effects of Pm and evaluate clinical, necroscopic and histopathological alterations in nonintoxicated rats treated with these solutions. AE and MT groups were formed of 10 animals each which received the extract in doses of 0.4g/kg, 0.8g/kg, 2g/kg and 4g/kg, intragastrically. The UD6EA and UD30EA groups were formed of five rats each, which received respectively 1 mL of Pm 6CH e Pm 30CH solutions, by oral route, three times a day, for eight days. Then, they were intoxicated with 2g/kg of Pm aqueous extract, intragastrically and received the correspondent ultra-highly diluted and dynamized preparations hourly until death. The UD6 and UD30 groups were formed of five rats each and received respectively 1 mL of Pm 6CH and Pm 30CH, orally, three times a day for 63 days. The animals were evaluated using clinical parameters, including the direct observation of their behavior at the open field and at the elevated plus-maze, and the study of the macro and microscopic lesions. Hyperacute death occurred after administration of the doses of 0.4g/kg, 0.8g/kg, 2g/kg e 4g/kg. In the AE group, prostration and nervous hyperexcitability followed or not by convulsive crisis were observed. In the MT group, the animals presented marked nervous depression without convulsion. Hepatic congestion and evidence of cardiac dilation were observed in the necropsy. Hydropic vacuolar degeneration of the renal distal convoluted tubules and congestion of several organs were observed in the histopathological examination. The latencies for the emergence of the first clinical signs, the convulsions and death occurrence were different in the animals of groups AE1 (Pm 2g/kg), UD6EA and UD30EA, but was considered inadequate the animals amount. Groups UD6 and UD30 did not show any clinical, behavioral, necroscopical or histopathological differences when compared to the control group. The conclusions were that the aqueous extract causes nervous excitability and convulsions while the mother tincture causes nervous depression. There is cumulative effect of the toxic substances present in the plant, and there is not evidence that the ultra-highly diluted and dynamized preparation increases the tolerance to Pm intoxication.A Palicourea marcgravii (Pm), a planta t?xica mais importante no Brasil, ? respons?vel por cerca da metade das mortes por intoxica??o natural em bovinos no Pa?s. A intoxica??o cursa com evolu??o superaguda e morte s?bita, atribu?da ao ?cido monofluoroac?tico, subst?ncia de alta toxidez para diversos mam?feros, inclusive o homem. A terap?utica homeop?tica emprega solu??es ultradilu?das e dinamizadas e h? evid?ncias suficientes para explor?-la como poss?vel prote??o contra intoxica??es. Os objetivos deste trabalho foram comparar os efeitos t?xicos dos extratos aquoso (EA) e hidro-alco?lico (tintura-m?e - TM) de Pm em ratos e avaliar o efeito das solu??es ultradilu?das e dinamizadas (Pm 6CH e Pm 30CH) sobre a toler?ncia e a??o t?xica da planta e avaliar a ocorr?ncia de altera??es cl?nicas, necrosc?picas e histopatol?gicas em ratos n?o-intoxicados tratados com essas solu??es. O EA, a TM, a Pm 6CH e a Pm 30CH foram administrados a 49 ratos distribu?dos em seis grupos-teste; dois grupos adicionais foram mantidos como controle. Os grupos EA e TM foram constitu?dos por 10 animais cada, que receberam doses que variaram de 0,4g/kg, 0,8g/kg, 2g/kg e 4g/kg. Os grupos UD6EA e UD30EA foram constitu?dos por cinco ratos cada e receberam 1mL das solu??es Pm 6CH e Pm 30CH, respectivamente, por via oral, tr?s vezes ao dia, e ap?s oito dias 2g/kg do extrato aquoso de Pm, por via intrag?strica. Os grupos UD6 e UD30, constitu?dos por cinco ratos cada, receberam 1mL de Pm 6CH e Pm 30CH, respectivamente, por via oral, tr?s vezes ao dia, durante 63 dias. Os animais foram avaliados por par?metros cl?nicos, comportamentais (campo aberto e labirinto em cruz elevado), achados de necropsia e exames histopatol?gicos. Ocorreu morte superaguda com as doses de 0,4g/kg, 0,8g/kg, 2g/kg e 4g/kg. No grupo EA observaram-se prostra??o e hiperexcitabilidade nervosa, seguida, ou n?o, por crise convulsiva e no grupo TM depress?o nervosa acentuada, sem convuls?o. Havia ainda p?los eri?ados, dispn?ia, cianose de extremidades, e hipotermia. ? necropsia observaram-se f?gado congesto e dilata??o card?aca e pelo exame histopatol?gico, degenera??o hidr?picovacuolar em t?bulos contornados distais dos rins e congest?o em v?rios ?rg?os. Houve diferen?as nas lat?ncias para o aparecimento dos primeiros sinais cl?nicos, de convuls?o e ocorr?ncia de morte entres os grupos UD30EA, UD6EA e EA1 (Pm 2g/kg), mas o numero de animais foi considerado pequeno para se atribuir efeito protetor das solu??es ultradilu?das e dinamizadas ? intoxica??o pela planta. Os grupos UD6 e UD30 n?o apresentaram diferen?as cl?nicas, comportamentais ou necrosc?picas em compara??o com o grupo controle. Conclui-se que o extrato aquoso produz excitabilidade nervosa e convuls?o e a tintura-m?e depress?o nervosa, que h? efeito acumulativo e que as solu??es ultradilu?das e dinamizadas n?o induziram aumento ponderal da toler?ncia aos efeitos t?xicos da Pm e n?o produziram altera??es no estado de sa?de dos animais sadios.Made available in DSpace on 2016-04-28T20:16:25Z (GMT). No. of bitstreams: 1 2007 - Luiz Figueira Pinto.pdf: 1581116 bytes, checksum: 36931ba33454d8c934e6419c55b06974 (MD5) Previous issue date: 2007-12-13application/pdfhttps://tede.ufrrj.br/retrieve/59948/2007%20-%20Luiz%20Figueira%20Pinto.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Ci?ncias Veterin?riasUFRRJBrasilInstituto de Veterin?riaPalicourea marcgraviiultradilu?dos?cido monofluoroac?tico.ultra-highly dilutedmonofluoroacetic acidMedicina Veterin?riaEfeitos dos extratos aquoso e hidro-alco?lico e das solu??es ultradilu?das de Palicourea marcgravii (Rubiaceae) em ratosEffects of aqueous and hydroalcoholic extracts and ultra-highly diluted solutions of Palicourea marcgravii (Ribiaciae) in ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRRJinstname:Universidade Federal Rural do Rio de Janeiro (UFRRJ)instacron:UFRRJTHUMBNAIL2007 - Luiz Figueira Pinto.pdf.jpg2007 - Luiz Figueira Pinto.pdf.jpgimage/jpeg1943http://localhost:8080/tede/bitstream/tede/822/3/2007+-+Luiz+Figueira+Pinto.pdf.jpgcc73c4c239a4c332d642ba1e7c7a9fb2MD53TEXT2007 - Luiz Figueira Pinto.pdf.txt2007 - Luiz Figueira Pinto.pdf.txttext/plain361937http://localhost:8080/tede/bitstream/tede/822/2/2007+-+Luiz+Figueira+Pinto.pdf.txt44f864ebd3a83397f0ec3d9d888cd6b5MD52ORIGINAL2007 - Luiz Figueira Pinto.pdf2007 - Luiz Figueira Pinto.pdfapplication/pdf1581374http://localhost:8080/tede/bitstream/tede/822/1/2007+-+Luiz+Figueira+Pinto.pdf46da6f8aeaddc2fcd6797d2a69fb0554MD51tede/8222022-06-14 11:46:39.5oai:localhost:tede/822Biblioteca Digital de Teses e Dissertaçõeshttps://tede.ufrrj.br/PUBhttps://tede.ufrrj.br/oai/requestbibliot@ufrrj.br||bibliot@ufrrj.bropendoar:2022-06-14T14:46:39Biblioteca Digital de Teses e Dissertações da UFRRJ - Universidade Federal Rural do Rio de Janeiro (UFRRJ)false |
dc.title.por.fl_str_mv |
Efeitos dos extratos aquoso e hidro-alco?lico e das solu??es ultradilu?das de Palicourea marcgravii (Rubiaceae) em ratos |
dc.title.alternative.eng.fl_str_mv |
Effects of aqueous and hydroalcoholic extracts and ultra-highly diluted solutions of Palicourea marcgravii (Ribiaciae) in rats |
title |
Efeitos dos extratos aquoso e hidro-alco?lico e das solu??es ultradilu?das de Palicourea marcgravii (Rubiaceae) em ratos |
spellingShingle |
Efeitos dos extratos aquoso e hidro-alco?lico e das solu??es ultradilu?das de Palicourea marcgravii (Rubiaceae) em ratos Pinto, Luiz Figueira Palicourea marcgravii ultradilu?dos ?cido monofluoroac?tico. ultra-highly diluted monofluoroacetic acid Medicina Veterin?ria |
title_short |
Efeitos dos extratos aquoso e hidro-alco?lico e das solu??es ultradilu?das de Palicourea marcgravii (Rubiaceae) em ratos |
title_full |
Efeitos dos extratos aquoso e hidro-alco?lico e das solu??es ultradilu?das de Palicourea marcgravii (Rubiaceae) em ratos |
title_fullStr |
Efeitos dos extratos aquoso e hidro-alco?lico e das solu??es ultradilu?das de Palicourea marcgravii (Rubiaceae) em ratos |
title_full_unstemmed |
Efeitos dos extratos aquoso e hidro-alco?lico e das solu??es ultradilu?das de Palicourea marcgravii (Rubiaceae) em ratos |
title_sort |
Efeitos dos extratos aquoso e hidro-alco?lico e das solu??es ultradilu?das de Palicourea marcgravii (Rubiaceae) em ratos |
author |
Pinto, Luiz Figueira |
author_facet |
Pinto, Luiz Figueira |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Peixoto, Paulo Fernando de Vargas |
dc.contributor.advisor1ID.fl_str_mv |
270.761.210-34 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3002512195318611 |
dc.contributor.advisor-co1.fl_str_mv |
Fran?a, Ticiana do Nascimento |
dc.contributor.advisor-co1ID.fl_str_mv |
027.897.197-05 |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/3545873525091315 |
dc.contributor.advisor-co2.fl_str_mv |
Brito, Marilene de Farias |
dc.contributor.advisor-co2ID.fl_str_mv |
211.517.924-20 |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://lattes.cnpq.br/7980824063026281 |
dc.contributor.authorID.fl_str_mv |
417.717.377-00 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2778797536334256 |
dc.contributor.author.fl_str_mv |
Pinto, Luiz Figueira |
contributor_str_mv |
Peixoto, Paulo Fernando de Vargas Fran?a, Ticiana do Nascimento Brito, Marilene de Farias |
dc.subject.por.fl_str_mv |
Palicourea marcgravii ultradilu?dos ?cido monofluoroac?tico. |
topic |
Palicourea marcgravii ultradilu?dos ?cido monofluoroac?tico. ultra-highly diluted monofluoroacetic acid Medicina Veterin?ria |
dc.subject.eng.fl_str_mv |
ultra-highly diluted monofluoroacetic acid |
dc.subject.cnpq.fl_str_mv |
Medicina Veterin?ria |
description |
Palicourea marcgravii (Pm) is the most important toxic plant in Brazil. It is responsible for about half of all bovine deaths by natural poisoning in the country. The poisoning has a hyperacute evolution resulting in sudden death, which is attributed to the monofluoroacetic acid. This substance has high toxicity to several mammals, including humans. The homeopathic therapeutics uses ultra-highly diluted and dynamized solutions and there is enough evidence to investigate them as possible protection against poisonings. The aims of the present work were to compare the toxic effects of aqueous (AE) and hydroalcoholic (mother tincture - MT) extracts of Pm in rats, evaluate the effects of ultra-highly diluted and dynamized solutions (Pm 6CH and Pm 30CH) over the development of tolerance to the toxic effects of Pm and evaluate clinical, necroscopic and histopathological alterations in nonintoxicated rats treated with these solutions. AE and MT groups were formed of 10 animals each which received the extract in doses of 0.4g/kg, 0.8g/kg, 2g/kg and 4g/kg, intragastrically. The UD6EA and UD30EA groups were formed of five rats each, which received respectively 1 mL of Pm 6CH e Pm 30CH solutions, by oral route, three times a day, for eight days. Then, they were intoxicated with 2g/kg of Pm aqueous extract, intragastrically and received the correspondent ultra-highly diluted and dynamized preparations hourly until death. The UD6 and UD30 groups were formed of five rats each and received respectively 1 mL of Pm 6CH and Pm 30CH, orally, three times a day for 63 days. The animals were evaluated using clinical parameters, including the direct observation of their behavior at the open field and at the elevated plus-maze, and the study of the macro and microscopic lesions. Hyperacute death occurred after administration of the doses of 0.4g/kg, 0.8g/kg, 2g/kg e 4g/kg. In the AE group, prostration and nervous hyperexcitability followed or not by convulsive crisis were observed. In the MT group, the animals presented marked nervous depression without convulsion. Hepatic congestion and evidence of cardiac dilation were observed in the necropsy. Hydropic vacuolar degeneration of the renal distal convoluted tubules and congestion of several organs were observed in the histopathological examination. The latencies for the emergence of the first clinical signs, the convulsions and death occurrence were different in the animals of groups AE1 (Pm 2g/kg), UD6EA and UD30EA, but was considered inadequate the animals amount. Groups UD6 and UD30 did not show any clinical, behavioral, necroscopical or histopathological differences when compared to the control group. The conclusions were that the aqueous extract causes nervous excitability and convulsions while the mother tincture causes nervous depression. There is cumulative effect of the toxic substances present in the plant, and there is not evidence that the ultra-highly diluted and dynamized preparation increases the tolerance to Pm intoxication. |
publishDate |
2007 |
dc.date.issued.fl_str_mv |
2007-12-13 |
dc.date.available.fl_str_mv |
2008-06-10 |
dc.date.accessioned.fl_str_mv |
2016-04-28T20:16:25Z |
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info:eu-repo/semantics/publishedVersion |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
PINTO, Luiz Figueira. Efeitos dos extratos aquoso e hidro-alco?lico e das solu??es ultradilu?das de Palicourea marcgravii (Rubiaceae) em ratos. 2007. 209 f. Tese (Doutorado em Ci?ncias Veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2007. |
dc.identifier.uri.fl_str_mv |
https://tede.ufrrj.br/jspui/handle/tede/822 |
identifier_str_mv |
PINTO, Luiz Figueira. Efeitos dos extratos aquoso e hidro-alco?lico e das solu??es ultradilu?das de Palicourea marcgravii (Rubiaceae) em ratos. 2007. 209 f. Tese (Doutorado em Ci?ncias Veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2007. |
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https://tede.ufrrj.br/jspui/handle/tede/822 |
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por |
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Universidade Federal Rural do Rio de Janeiro |
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Programa de P?s-Gradua??o em Ci?ncias Veterin?rias |
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UFRRJ |
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Brasil |
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Instituto de Veterin?ria |
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Universidade Federal Rural do Rio de Janeiro |
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