Caracteriza??o fenot?pica e produ??o de biopol?meros por bact?rias isoladas de n?dulos de guandu [(Cajanus cajan (L.) Millsp.]
Ano de defesa: | 2009 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal Rural do Rio de Janeiro
|
Programa de Pós-Graduação: |
Programa de P?s-Gradua??o em Agronomia - Ci?ncia do Solo
|
Departamento: |
Instituto de Agronomia
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | https://tede.ufrrj.br/jspui/handle/tede/334 |
Resumo: | The biological nitrogen fixation (BNF) is a natural process carried out by prokaryotes collectively called diazotrophs. Among the diverse microorganisms able to fix the atmospheric nitrogen, stand out those capable of set up mutualistic associations with legume plants in specialized structures called nodules. Thus the search for microorganisms able to establish efficient associations with plants of legume family had been the target of studies for the last decades. In recent years, further the prospecting for microorganisms able to establish efficient associations, the rhizobia have been the target of other biotechnology interests, such as the production of molecules of industrial interest, such as enzymes and biopolymers. These macromolecules are used in various branches of industry and the constant search for microorganisms able to produce new molecules is a source of innovation in these sectors. In this context, the aim of this study was to evaluate the phenotypic diversity of bacterial isolates from root nodules of three pigeonpea genotypes, as well as to evaluate the capacity of these bacterial isolates to produce exopolysaccharides, polyhydroxybutyrate and amylases. Eight soils were sampled four in Serop?dica municipality (two samples were from Dystric Udult soils), and two from a Haplic Fragiudult soil), four in Pinheiral municipality (Xanthic Udox soil, Aqult soil, Rhodic Udult, and a Fluvaquent), all in Rio de Janeiro State, to set up a experiment to obtain the bacterial isolates. The experiment was set up at a factorial design of 3 (genotypes) x 8 (soils). The plants were harvest at 75 days after the emergence and the nodulation was evaluated. Five nodules for each plant were superficially disinfected and crushed in Petri dishes with YMA media containing Congo Red stain. After the purification of the bacterial isolates it was proceeded the cultural characterization, and evaluated the capacity of the bacterial strains to grow in culture media containing eight different carbon sources, antibiotics or salts and different temperatures. The isolates were authenticated in greenhouse sterile conditions and an experiment was carried out to evaluate the capacity of selected isolates to establish the symbiosis. The isolates presented a very diverse pattern to present intrinsic resistance to antibiotics and to grow eighth different carbon sources. It was also evaluated the capacity of the isolates to produce polyhydroxybutyrate, exopolysaccharides and amylase. The reology of selected EPS were also evaluated. The majority of isolates tested were able to present growth when incubated at 42?C. Part of the isolates was able to grow in culture media with 3% of NaCl. Though 34% of the isolates were not able to nodulate the host plant, and from the isolates evaluated to establish the symbiosis all presented the same nodulation compared with the recommended strains. The isolates varied a lot regarding the production of PHB and EPS, showing strong influence of the carbon source used. The EPS rheology showed pseudoplastic behavior. Regarding the amylase production, one isolate had reached a degradation ha lo 2.7 times the colony size. These results show the biotechnological potential of these isolates, both for inoculants production and biopolymer production. |
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Xavier, Gustavo Ribeiro032.488.987-98gustavo@cnpab.embrapa.brOliveira, Paulo Jansen de842.254.837-20http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723548P9Rumjanek, Norma Gouv?a345.536.817-49norma@cnpab.embrapa.br051.958.526-77http://lattes.cnpq.br/5149478274259227Fernandes J?nior, Paulo Ivan2016-04-26T19:39:36Z2011-02-172009-11-27FERNANDES J?NIOR, Paulo Ivan. Caracteriza??o fenot?pica e produ??o de biopol?meros por bact?rias isoladas de n?dulos de guandu (Cajanus cajan (L.) Millsp.]. 2009. 183 f. Tese (Doutorado em Agronomia, Ci?ncia do Solo) - Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2009.https://tede.ufrrj.br/jspui/handle/tede/334The biological nitrogen fixation (BNF) is a natural process carried out by prokaryotes collectively called diazotrophs. Among the diverse microorganisms able to fix the atmospheric nitrogen, stand out those capable of set up mutualistic associations with legume plants in specialized structures called nodules. Thus the search for microorganisms able to establish efficient associations with plants of legume family had been the target of studies for the last decades. In recent years, further the prospecting for microorganisms able to establish efficient associations, the rhizobia have been the target of other biotechnology interests, such as the production of molecules of industrial interest, such as enzymes and biopolymers. These macromolecules are used in various branches of industry and the constant search for microorganisms able to produce new molecules is a source of innovation in these sectors. In this context, the aim of this study was to evaluate the phenotypic diversity of bacterial isolates from root nodules of three pigeonpea genotypes, as well as to evaluate the capacity of these bacterial isolates to produce exopolysaccharides, polyhydroxybutyrate and amylases. Eight soils were sampled four in Serop?dica municipality (two samples were from Dystric Udult soils), and two from a Haplic Fragiudult soil), four in Pinheiral municipality (Xanthic Udox soil, Aqult soil, Rhodic Udult, and a Fluvaquent), all in Rio de Janeiro State, to set up a experiment to obtain the bacterial isolates. The experiment was set up at a factorial design of 3 (genotypes) x 8 (soils). The plants were harvest at 75 days after the emergence and the nodulation was evaluated. Five nodules for each plant were superficially disinfected and crushed in Petri dishes with YMA media containing Congo Red stain. After the purification of the bacterial isolates it was proceeded the cultural characterization, and evaluated the capacity of the bacterial strains to grow in culture media containing eight different carbon sources, antibiotics or salts and different temperatures. The isolates were authenticated in greenhouse sterile conditions and an experiment was carried out to evaluate the capacity of selected isolates to establish the symbiosis. The isolates presented a very diverse pattern to present intrinsic resistance to antibiotics and to grow eighth different carbon sources. It was also evaluated the capacity of the isolates to produce polyhydroxybutyrate, exopolysaccharides and amylase. The reology of selected EPS were also evaluated. The majority of isolates tested were able to present growth when incubated at 42?C. Part of the isolates was able to grow in culture media with 3% of NaCl. Though 34% of the isolates were not able to nodulate the host plant, and from the isolates evaluated to establish the symbiosis all presented the same nodulation compared with the recommended strains. The isolates varied a lot regarding the production of PHB and EPS, showing strong influence of the carbon source used. The EPS rheology showed pseudoplastic behavior. Regarding the amylase production, one isolate had reached a degradation ha lo 2.7 times the colony size. These results show the biotechnological potential of these isolates, both for inoculants production and biopolymer production.A fixa??o biol?gica do nitrog?nio ? um processo natural realizado por procariotos denominados diazotr?ficos. Dentre os diversos microrganismos capazes de fixar o nitrog?nio, destacam-se aqueles capazes de estabelecer associa??es mutual?sticas com leguminosas em estruturas especializadas denominadas n?dulos. A busca por microrganismos capazes de estabelecer uma associa??o eficiente com as plantas desta fam?lia tem sido alvo de estudos ao longo das ?ltimas d?cadas. Nos ?ltimos anos, al?m da prospec??o por microrganismos capazes de estabelecer associa??es eficientes, os riz?bio tem sido alvo de outros interesses biotecnol?gicos como a produ??o de mol?culas de interesse industrial, como enzimas e biopol?meros. Estas macromol?culas s?o empregadas em diversos ramos da ind?stria e a busca por novos microrganismos capazes de produzir essas mol?culas constitui uma fonte de inova??o constante nestes setores. Neste contexto, o objetivo deste trabalho fo i avaliar a diversidade fenot?pica de bact?rias isoladas de n?dulos de tr?s cultivares de guandu, bem como avaliar a capacidade desses isolados em produzir exopolissacar?deos, polihidroxibutirato e amilases. Foram coletados amostras de oito solos, 4 em Serop?dica (duas amostras em ?reas com Argissolo Vermelho Amarelo e duas ?reas com Planossolo H?plico) e 4 em Pinheiral (Latossolo Amarelo; Argissolo Vermelho; Argissolo Acinzentado e Neossolo Fl?vico) para implanta??o do experimento de para a obten??o das bact?rias. O experimento foi montado no esquema fatorial 3 (cultivares) x 8 (solos). As plantas foram colhidas aos 75 dias ap?s a emerg?ncia e a nodula??o foi avaliada. Os n?dulos foram desinfestados superficialmente e pressionados em placas de Petri contendo meio de cultura YMA com Vermelho Congo. Ap?s purifica??o dos isolados foi realizada a caracteriza??o cultural e foi avaliada a capacidade crescimento em meio com diferentes fontes de carbono, concentra??es de antibi?ticos ou de sais e diferentes temperaturas. Os isolados foram validados em condi??es est?reis de casa de vegeta??o e a foi realizado um teste para avaliar a capacidade de estabelecimento da simbiose. Paralelamente foram selecionados isolados para avaliar a capacidade de produ??o de polihidroxibutirato (PHB), exopolisacar?deos (EPS), e amilase. A reologia de EPS selecionados tamb?m foi avaliada. Os isolados apresentaram um padr?o muito diverso de capacidade de metabolizar diferentes fontes de carbono e de apresentar resist?ncia intr?nseca a antibi?ticos. A maioria dos isolados testados foram capazes de crescer quando incubados a temperaturas de 42?C. Grande parte dos isolados apresentou resist?ncia ? salinidade (3% de NaCl). J? 34% dos isolados n?o foram capazes de nodular a planta hospedeira e dos isolados avaliados para o estabelecimento da simbiose, todos apresentaram nodula??o estatisticamente igual ?quela apresentada pelas estirpes recomendadas. Os isolados variaram quanto ? capacidade de produzir EPS e PHB, havendo grande influ?ncia da fonte de carbono utilizada. A reologia dos EPS demonstrou comportamento pseudopl?stico das solu??es de EPS selecionados. Com rela??o ? produ??o de amilases, os um isolado se destacou bastante dos demais, onde a halo de degrada??o do amido atingiu mais de 2,7 vezes o tamanho da col?nia. Estes resultados demonstram o potencial biotecnol?gico dessas bact?rias, tanto para a produ??o de inoculantes como para a produ??o de biopol?meros.Made available in DSpace on 2016-04-26T19:39:36Z (GMT). No. of bitstreams: 1 2009 - Paulo Ivan Fernandes J?nior.pdf: 3506552 bytes, checksum: 8f231cd05ee418023b1525472d18ba46 (MD5) Previous issue date: 2009-11-27application/pdfhttps://tede.ufrrj.br/retrieve/61136/2009%20-%20Paulo%20Ivan%20Fernandes%20J%c3%banior.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Agronomia - Ci?ncia do SoloUFRRJBrasilInstituto de Agronomiafixa??o biol?gica de nitrog?niopolihidroxibutiratoexopolissacar?deosinoculantediversidadeinova??o tecnol?gicabiological nitrogen fixationpolyhydroxybutyrateexopolisaccharidesamylasediversitytechnological innovationinoculantAgronomiaCaracteriza??o fenot?pica e produ??o de biopol?meros por bact?rias isoladas de n?dulos de guandu [(Cajanus cajan (L.) Millsp.]Phenotypic characterization and biopolymers production by bacteria isolated from pigeonpea [Cajanus cajan (L.) Millsp.] root nodulesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRRJinstname:Universidade Federal Rural do Rio de Janeiro (UFRRJ)instacron:UFRRJTHUMBNAIL2009 - Paulo Ivan Fernandes J?nior.pdf.jpg2009 - Paulo Ivan Fernandes J?nior.pdf.jpgimage/jpeg1943http://localhost:8080/tede/bitstream/tede/334/17/2009+-+Paulo+Ivan+Fernandes+J%C3%BAnior.pdf.jpg8c1280dca4077478b5fad29883580c72MD517TEXT2009 - Paulo Ivan Fernandes J?nior.pdf.txt2009 - Paulo Ivan Fernandes J?nior.pdf.txttext/plain477411http://localhost:8080/tede/bitstream/tede/334/16/2009+-+Paulo+Ivan+Fernandes+J%C3%BAnior.pdf.txt826fde4301d3412764b51800d25deffeMD516ORIGINAL2009 - Paulo Ivan Fernandes J?nior.pdf2009 - Paulo Ivan Fernandes J?nior.pdfapplication/pdf3506701http://localhost:8080/tede/bitstream/tede/334/1/2009+-+Paulo+Ivan+Fernandes+J%C3%BAnior.pdf5467a25a9600beb6ea82659265ea4559MD51tede/3342022-06-24 11:59:18.495oai:localhost:tede/334Biblioteca Digital de Teses e Dissertaçõeshttps://tede.ufrrj.br/PUBhttps://tede.ufrrj.br/oai/requestbibliot@ufrrj.br||bibliot@ufrrj.bropendoar:2022-06-24T14:59:18Biblioteca Digital de Teses e Dissertações da UFRRJ - Universidade Federal Rural do Rio de Janeiro (UFRRJ)false |
dc.title.por.fl_str_mv |
Caracteriza??o fenot?pica e produ??o de biopol?meros por bact?rias isoladas de n?dulos de guandu [(Cajanus cajan (L.) Millsp.] |
dc.title.alternative.eng.fl_str_mv |
Phenotypic characterization and biopolymers production by bacteria isolated from pigeonpea [Cajanus cajan (L.) Millsp.] root nodules |
title |
Caracteriza??o fenot?pica e produ??o de biopol?meros por bact?rias isoladas de n?dulos de guandu [(Cajanus cajan (L.) Millsp.] |
spellingShingle |
Caracteriza??o fenot?pica e produ??o de biopol?meros por bact?rias isoladas de n?dulos de guandu [(Cajanus cajan (L.) Millsp.] Fernandes J?nior, Paulo Ivan fixa??o biol?gica de nitrog?nio polihidroxibutirato exopolissacar?deos inoculante diversidade inova??o tecnol?gica biological nitrogen fixation polyhydroxybutyrate exopolisaccharides amylase diversity technological innovation inoculant Agronomia |
title_short |
Caracteriza??o fenot?pica e produ??o de biopol?meros por bact?rias isoladas de n?dulos de guandu [(Cajanus cajan (L.) Millsp.] |
title_full |
Caracteriza??o fenot?pica e produ??o de biopol?meros por bact?rias isoladas de n?dulos de guandu [(Cajanus cajan (L.) Millsp.] |
title_fullStr |
Caracteriza??o fenot?pica e produ??o de biopol?meros por bact?rias isoladas de n?dulos de guandu [(Cajanus cajan (L.) Millsp.] |
title_full_unstemmed |
Caracteriza??o fenot?pica e produ??o de biopol?meros por bact?rias isoladas de n?dulos de guandu [(Cajanus cajan (L.) Millsp.] |
title_sort |
Caracteriza??o fenot?pica e produ??o de biopol?meros por bact?rias isoladas de n?dulos de guandu [(Cajanus cajan (L.) Millsp.] |
author |
Fernandes J?nior, Paulo Ivan |
author_facet |
Fernandes J?nior, Paulo Ivan |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Xavier, Gustavo Ribeiro |
dc.contributor.advisor1ID.fl_str_mv |
032.488.987-98 |
dc.contributor.advisor1Lattes.fl_str_mv |
gustavo@cnpab.embrapa.br |
dc.contributor.advisor-co1.fl_str_mv |
Oliveira, Paulo Jansen de |
dc.contributor.advisor-co1ID.fl_str_mv |
842.254.837-20 |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723548P9 |
dc.contributor.advisor-co2.fl_str_mv |
Rumjanek, Norma Gouv?a |
dc.contributor.advisor-co2ID.fl_str_mv |
345.536.817-49 |
dc.contributor.advisor-co2Lattes.fl_str_mv |
norma@cnpab.embrapa.br |
dc.contributor.authorID.fl_str_mv |
051.958.526-77 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5149478274259227 |
dc.contributor.author.fl_str_mv |
Fernandes J?nior, Paulo Ivan |
contributor_str_mv |
Xavier, Gustavo Ribeiro Oliveira, Paulo Jansen de Rumjanek, Norma Gouv?a |
dc.subject.por.fl_str_mv |
fixa??o biol?gica de nitrog?nio polihidroxibutirato exopolissacar?deos inoculante diversidade inova??o tecnol?gica |
topic |
fixa??o biol?gica de nitrog?nio polihidroxibutirato exopolissacar?deos inoculante diversidade inova??o tecnol?gica biological nitrogen fixation polyhydroxybutyrate exopolisaccharides amylase diversity technological innovation inoculant Agronomia |
dc.subject.eng.fl_str_mv |
biological nitrogen fixation polyhydroxybutyrate exopolisaccharides amylase diversity technological innovation inoculant |
dc.subject.cnpq.fl_str_mv |
Agronomia |
description |
The biological nitrogen fixation (BNF) is a natural process carried out by prokaryotes collectively called diazotrophs. Among the diverse microorganisms able to fix the atmospheric nitrogen, stand out those capable of set up mutualistic associations with legume plants in specialized structures called nodules. Thus the search for microorganisms able to establish efficient associations with plants of legume family had been the target of studies for the last decades. In recent years, further the prospecting for microorganisms able to establish efficient associations, the rhizobia have been the target of other biotechnology interests, such as the production of molecules of industrial interest, such as enzymes and biopolymers. These macromolecules are used in various branches of industry and the constant search for microorganisms able to produce new molecules is a source of innovation in these sectors. In this context, the aim of this study was to evaluate the phenotypic diversity of bacterial isolates from root nodules of three pigeonpea genotypes, as well as to evaluate the capacity of these bacterial isolates to produce exopolysaccharides, polyhydroxybutyrate and amylases. Eight soils were sampled four in Serop?dica municipality (two samples were from Dystric Udult soils), and two from a Haplic Fragiudult soil), four in Pinheiral municipality (Xanthic Udox soil, Aqult soil, Rhodic Udult, and a Fluvaquent), all in Rio de Janeiro State, to set up a experiment to obtain the bacterial isolates. The experiment was set up at a factorial design of 3 (genotypes) x 8 (soils). The plants were harvest at 75 days after the emergence and the nodulation was evaluated. Five nodules for each plant were superficially disinfected and crushed in Petri dishes with YMA media containing Congo Red stain. After the purification of the bacterial isolates it was proceeded the cultural characterization, and evaluated the capacity of the bacterial strains to grow in culture media containing eight different carbon sources, antibiotics or salts and different temperatures. The isolates were authenticated in greenhouse sterile conditions and an experiment was carried out to evaluate the capacity of selected isolates to establish the symbiosis. The isolates presented a very diverse pattern to present intrinsic resistance to antibiotics and to grow eighth different carbon sources. It was also evaluated the capacity of the isolates to produce polyhydroxybutyrate, exopolysaccharides and amylase. The reology of selected EPS were also evaluated. The majority of isolates tested were able to present growth when incubated at 42?C. Part of the isolates was able to grow in culture media with 3% of NaCl. Though 34% of the isolates were not able to nodulate the host plant, and from the isolates evaluated to establish the symbiosis all presented the same nodulation compared with the recommended strains. The isolates varied a lot regarding the production of PHB and EPS, showing strong influence of the carbon source used. The EPS rheology showed pseudoplastic behavior. Regarding the amylase production, one isolate had reached a degradation ha lo 2.7 times the colony size. These results show the biotechnological potential of these isolates, both for inoculants production and biopolymer production. |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009-11-27 |
dc.date.available.fl_str_mv |
2011-02-17 |
dc.date.accessioned.fl_str_mv |
2016-04-26T19:39:36Z |
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FERNANDES J?NIOR, Paulo Ivan. Caracteriza??o fenot?pica e produ??o de biopol?meros por bact?rias isoladas de n?dulos de guandu (Cajanus cajan (L.) Millsp.]. 2009. 183 f. Tese (Doutorado em Agronomia, Ci?ncia do Solo) - Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2009. |
dc.identifier.uri.fl_str_mv |
https://tede.ufrrj.br/jspui/handle/tede/334 |
identifier_str_mv |
FERNANDES J?NIOR, Paulo Ivan. Caracteriza??o fenot?pica e produ??o de biopol?meros por bact?rias isoladas de n?dulos de guandu (Cajanus cajan (L.) Millsp.]. 2009. 183 f. Tese (Doutorado em Agronomia, Ci?ncia do Solo) - Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2009. |
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https://tede.ufrrj.br/jspui/handle/tede/334 |
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por |
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Universidade Federal Rural do Rio de Janeiro |
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UFRRJ |
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Brasil |
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Universidade Federal Rural do Rio de Janeiro |
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