Potencial bioativo de extratos obtidos a partir do resíduo industrial do café torrado utilizando a técnica de ultrassom assistida
Ano de defesa: | 2022 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Não Informado pela instituição
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Programa de Pós-Graduação: |
Pós-Graduação em Ciência e Tecnologia de Alimentos
|
Departamento: |
Não Informado pela instituição
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País: |
Não Informado pela instituição
|
Palavras-chave em Português: | |
Link de acesso: | http://ri.ufs.br/jspui/handle/riufs/16215 |
Resumo: | The coffee bean is an agricultural product that makes one of the most consumed beverages and appreciated worldwide both for its aroma and its flavor. Brazil is the world's largest producer of coffee and, as a result, large amounts of agro-industrial residues are obtained during its processing, which are not used because they do not have high commercial value. This work aimed to optimize the process of extracting polyphenolic compounds from industrial coffee residue using an assisted ultrasound technique. In the experiments, the ethanol concentration, liquid/solid ratio and extraction time were varied through the central composite rotational design. The extracts obtained were analyzed to total phenolics and total flavonoids compounds, antioxidant activity by DPPH, FRAP and ABTS methods and antimicrobial activity. Maximum extraction of total phenolics compounds can be obtained when ethanol concentration between 27 and 50%, extraction time between 20 and 35 min and liquid/solid ratio between 42 and 50.00 mL/g were used. For the extraction of total flavonoids, maximum values can be obtained when ethanol concentration between 20 and 27%, liquid/solid ratio between 10 mL/g to 12 mL/g and 48 mL/g to 50 mL/g and extraction times between 20 and 120 min were used. The antioxidant activity was maximum when liquid/solid ratio between 41 and 48 mL/g, extraction time between 115 and 120 min and ethanol concentration between 35% and 46% were used. The antibacterial activity of the extracts was analyzed in vitro. Gram-negative bacteria were sensitive to the extracts, being Pseudomonas aeruginosa, that showed greater sensitivity to more extracts followed by Serratia marcescens and Escherichia coli bacteria. Among the gram-positive bacteria, Staphylococcus aureus was sensitive. The extraction of compounds from the industrial residue of coffee by the assisted ultrasound technique was optimized, demonstrating the potential of the extracts to be used in future applications as a source of bioactive compounds. |
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Andrade, Sonja de FrançaSantana, Luciana Cristina Lins Aquino2022-08-25T19:07:42Z2022-08-25T19:07:42Z2022-01-22ANDRADE, Sonja de França. Potencial bioativo de extratos obtidos a partir do resíduo industrial do café torrado utilizando a técnica de ultrassom assistida. 2022. 65 f. Dissertação (Mestrado em Ciência e Tecnologia de Alimentos) – Universidade Federal de Sergipe, São Cristóvão, 2022.http://ri.ufs.br/jspui/handle/riufs/16215The coffee bean is an agricultural product that makes one of the most consumed beverages and appreciated worldwide both for its aroma and its flavor. Brazil is the world's largest producer of coffee and, as a result, large amounts of agro-industrial residues are obtained during its processing, which are not used because they do not have high commercial value. This work aimed to optimize the process of extracting polyphenolic compounds from industrial coffee residue using an assisted ultrasound technique. In the experiments, the ethanol concentration, liquid/solid ratio and extraction time were varied through the central composite rotational design. The extracts obtained were analyzed to total phenolics and total flavonoids compounds, antioxidant activity by DPPH, FRAP and ABTS methods and antimicrobial activity. Maximum extraction of total phenolics compounds can be obtained when ethanol concentration between 27 and 50%, extraction time between 20 and 35 min and liquid/solid ratio between 42 and 50.00 mL/g were used. For the extraction of total flavonoids, maximum values can be obtained when ethanol concentration between 20 and 27%, liquid/solid ratio between 10 mL/g to 12 mL/g and 48 mL/g to 50 mL/g and extraction times between 20 and 120 min were used. The antioxidant activity was maximum when liquid/solid ratio between 41 and 48 mL/g, extraction time between 115 and 120 min and ethanol concentration between 35% and 46% were used. The antibacterial activity of the extracts was analyzed in vitro. Gram-negative bacteria were sensitive to the extracts, being Pseudomonas aeruginosa, that showed greater sensitivity to more extracts followed by Serratia marcescens and Escherichia coli bacteria. Among the gram-positive bacteria, Staphylococcus aureus was sensitive. The extraction of compounds from the industrial residue of coffee by the assisted ultrasound technique was optimized, demonstrating the potential of the extracts to be used in future applications as a source of bioactive compounds.O grão do café é um produto agrícola que faz uma das bebidas mais consumidas e mundialmente apreciada tanto pelo aroma quanto pelo sabor. O Brasil é o maior produtor mundial de café e como consequência, se obtém grandes quantidades de resíduos agroindustriais durante o seu processamento, os quais não são amplamente aproveitados por não possuírem alto valor comercial. Este trabalho teve como objetivo a otimização do processo de extração de compostos polifenólicos a partir do resíduo industrial do café utilizando a técnica de ultrassom assistida. Nos experimentos variou-se a concentração de etanol, razão líquido/sólido e tempo de extração através do delineamento composto central rotacional. Os extratos obtidos foram analisados quanto aos teores de fenólicos totais e flavonoides totais, atividade antioxidante pelos métodos de DPPH, FRAP e ABTS e atividade antimicrobiana. Máxima extração de fenólicos totais pode ser obtida em concentrações de etanol entre 27 e 50%, tempo de extração entre 20 e 35 min e razão líquido/sólido entre 42 e 50,00 mL/g. Já para a extração de flavonoides totais valores máximos podem ser obtidos em concentrações de etanol entre 20 e 27%, razão líquido/ sólido entre 10 mL/g e 12 mL/g e de 48 mL/g à 50 mL/g e nos tempos de extração entre 20 e 120 min. A atividade antioxidante foi máxima quando utilizado razão líquido/ sólido entre 41 e 48 mL/g, tempo de extração entre 115 e 120 min e concentrações de etanol entre 35% e 46%. A atividade antibacteriana dos extratos foi analisada in vitro. As bactérias Gram-negativas foram sensíveis aos extratos sendo a Pseudomonas aeruginosa que demonstrou maior sensibilidade para o maior número de extratos seguido das bactérias Serratia marcescens e Escherichia coli. Entre as bactérias Gram-positivas, o Staphylococcus aureus apresentou sensibilidade. A extração de compostos do resíduo industrial do café pela técnica de ultrassom assistida foi otimizada demonstrando o potencial dos extratos para serem utilizados em futuras aplicações como fonte de compostos bioativos.São CristóvãoporCaféCompostos bioativos das plantasResíduos industriaisFenóisPotencial bioativo de extratos obtidos a partir do resíduo industrial do café torrado utilizando a técnica de ultrassom assistidainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisPós-Graduação em Ciência e Tecnologia de AlimentosUniversidade Federal de Sergipereponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain; charset=utf-81475https://ri.ufs.br/jspui/bitstream/riufs/16215/1/license.txt098cbbf65c2c15e1fb2e49c5d306a44cMD51ORIGINALSONJA_FRANCA_ANDRADE.pdfSONJA_FRANCA_ANDRADE.pdfapplication/pdf1502313https://ri.ufs.br/jspui/bitstream/riufs/16215/2/SONJA_FRANCA_ANDRADE.pdfbcd2142594a01bc8e31e17d0bb7e26aaMD52TEXTSONJA_FRANCA_ANDRADE.pdf.txtSONJA_FRANCA_ANDRADE.pdf.txtExtracted texttext/plain110367https://ri.ufs.br/jspui/bitstream/riufs/16215/3/SONJA_FRANCA_ANDRADE.pdf.txt87e1b957787eada9afa495a63f4d07aaMD53THUMBNAILSONJA_FRANCA_ANDRADE.pdf.jpgSONJA_FRANCA_ANDRADE.pdf.jpgGenerated Thumbnailimage/jpeg1381https://ri.ufs.br/jspui/bitstream/riufs/16215/4/SONJA_FRANCA_ANDRADE.pdf.jpg8d1c47a31a2449986263cb6345345244MD54riufs/162152022-08-25 16:07:53.881oai:ufs.br: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Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2022-08-25T19:07:53Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false |
dc.title.pt_BR.fl_str_mv |
Potencial bioativo de extratos obtidos a partir do resíduo industrial do café torrado utilizando a técnica de ultrassom assistida |
title |
Potencial bioativo de extratos obtidos a partir do resíduo industrial do café torrado utilizando a técnica de ultrassom assistida |
spellingShingle |
Potencial bioativo de extratos obtidos a partir do resíduo industrial do café torrado utilizando a técnica de ultrassom assistida Andrade, Sonja de França Café Compostos bioativos das plantas Resíduos industriais Fenóis |
title_short |
Potencial bioativo de extratos obtidos a partir do resíduo industrial do café torrado utilizando a técnica de ultrassom assistida |
title_full |
Potencial bioativo de extratos obtidos a partir do resíduo industrial do café torrado utilizando a técnica de ultrassom assistida |
title_fullStr |
Potencial bioativo de extratos obtidos a partir do resíduo industrial do café torrado utilizando a técnica de ultrassom assistida |
title_full_unstemmed |
Potencial bioativo de extratos obtidos a partir do resíduo industrial do café torrado utilizando a técnica de ultrassom assistida |
title_sort |
Potencial bioativo de extratos obtidos a partir do resíduo industrial do café torrado utilizando a técnica de ultrassom assistida |
author |
Andrade, Sonja de França |
author_facet |
Andrade, Sonja de França |
author_role |
author |
dc.contributor.author.fl_str_mv |
Andrade, Sonja de França |
dc.contributor.advisor1.fl_str_mv |
Santana, Luciana Cristina Lins Aquino |
contributor_str_mv |
Santana, Luciana Cristina Lins Aquino |
dc.subject.por.fl_str_mv |
Café Compostos bioativos das plantas Resíduos industriais Fenóis |
topic |
Café Compostos bioativos das plantas Resíduos industriais Fenóis |
description |
The coffee bean is an agricultural product that makes one of the most consumed beverages and appreciated worldwide both for its aroma and its flavor. Brazil is the world's largest producer of coffee and, as a result, large amounts of agro-industrial residues are obtained during its processing, which are not used because they do not have high commercial value. This work aimed to optimize the process of extracting polyphenolic compounds from industrial coffee residue using an assisted ultrasound technique. In the experiments, the ethanol concentration, liquid/solid ratio and extraction time were varied through the central composite rotational design. The extracts obtained were analyzed to total phenolics and total flavonoids compounds, antioxidant activity by DPPH, FRAP and ABTS methods and antimicrobial activity. Maximum extraction of total phenolics compounds can be obtained when ethanol concentration between 27 and 50%, extraction time between 20 and 35 min and liquid/solid ratio between 42 and 50.00 mL/g were used. For the extraction of total flavonoids, maximum values can be obtained when ethanol concentration between 20 and 27%, liquid/solid ratio between 10 mL/g to 12 mL/g and 48 mL/g to 50 mL/g and extraction times between 20 and 120 min were used. The antioxidant activity was maximum when liquid/solid ratio between 41 and 48 mL/g, extraction time between 115 and 120 min and ethanol concentration between 35% and 46% were used. The antibacterial activity of the extracts was analyzed in vitro. Gram-negative bacteria were sensitive to the extracts, being Pseudomonas aeruginosa, that showed greater sensitivity to more extracts followed by Serratia marcescens and Escherichia coli bacteria. Among the gram-positive bacteria, Staphylococcus aureus was sensitive. The extraction of compounds from the industrial residue of coffee by the assisted ultrasound technique was optimized, demonstrating the potential of the extracts to be used in future applications as a source of bioactive compounds. |
publishDate |
2022 |
dc.date.accessioned.fl_str_mv |
2022-08-25T19:07:42Z |
dc.date.available.fl_str_mv |
2022-08-25T19:07:42Z |
dc.date.issued.fl_str_mv |
2022-01-22 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ANDRADE, Sonja de França. Potencial bioativo de extratos obtidos a partir do resíduo industrial do café torrado utilizando a técnica de ultrassom assistida. 2022. 65 f. Dissertação (Mestrado em Ciência e Tecnologia de Alimentos) – Universidade Federal de Sergipe, São Cristóvão, 2022. |
dc.identifier.uri.fl_str_mv |
http://ri.ufs.br/jspui/handle/riufs/16215 |
identifier_str_mv |
ANDRADE, Sonja de França. Potencial bioativo de extratos obtidos a partir do resíduo industrial do café torrado utilizando a técnica de ultrassom assistida. 2022. 65 f. Dissertação (Mestrado em Ciência e Tecnologia de Alimentos) – Universidade Federal de Sergipe, São Cristóvão, 2022. |
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http://ri.ufs.br/jspui/handle/riufs/16215 |
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Pós-Graduação em Ciência e Tecnologia de Alimentos |
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Universidade Federal de Sergipe |
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