Dieta enriquecida com lipídeo associada à somatotropina recombinante bovina em novilhas superovuladas

Detalhes bibliográficos
Ano de defesa: 2021
Autor(a) principal: Oliveira, Richelle Matos
Orientador(a): Santos, Anselmo Domingos Ferreira
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Não Informado pela instituição
Programa de Pós-Graduação: Pós-Graduação Integrada em Zootecnia
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Área do conhecimento CNPq:
Link de acesso: https://ri.ufs.br/jspui/handle/riufs/15072
Resumo: The aim was to evaluate the effects of soybean oil supplementation associated with the administration of bovine recombinant somatotropin (rBST) on the population of ovarian follicles, standardization of the superovulation response, and embryo recovery. We used 40 Holstein heifers submitted to a 2x2 factorial, with two diets, without or with soybean oil supplementation; and the administration or not of rBST seven days after estrus, forming the experimental groups: DC-SL (control diet plus saline solution application); OL-SL (diet with soybean oil plus saline application); DC-rBST (control diet plus application of 320 mg rBST) e OL-rBST (diet with soybean oil plus the application of 320 mg rBST). The animals received two applications of 500 mg of PGF2α for estrus synchronization and were superovulated with 400 IU of FSH in decreasing daily doses for four consecutive days. On the day before the start of superovulation, the two largest follicles were punctured to quantify the intrafollicular concentration of high-density lipoprotein (HDL). Statistical analyzes were processed using the Action R statistical software version 3.0.2, using ANOVA and Fisher's Test (F test; two comparisons) for the mean characteristics with a probability level of 5%. There were no differences in dry matter intake, follicular activity, P4 concentration, and superovulation response. The group OL-rBST got a greater CL area (4.67±0.67 cm2) and a positive effect was evidenced for HDL concentration in the treatment groups OL-SL (40.86±9.8 mg/dL) and OL-rBST (53.67±10.35 mg/dL). Soybean oil supplementation in the diet associated with the administration of rBST did not provide uniformity in the superovulation response and the embryonic recovery of heifer.
id UFS-2_b1e2915f7e86710e38cbe9fa76c653ce
oai_identifier_str oai:ufs.br:riufs/15072
network_acronym_str UFS-2
network_name_str Repositório Institucional da UFS
repository_id_str
spelling Oliveira, Richelle MatosSantos, Anselmo Domingos Ferreira2022-02-23T18:18:48Z2022-02-23T18:18:48Z2021-10-29OLIVEIRA, Richelle Matos. Dieta enriquecida com lipídeo associada à somatotropina recombinante bovina em novilhas superovuladas. 2021. 38 f. Dissertação (Mestrado em Zootecnia) – Universidade Federal de Sergipe, São Cristóvão, 2021.https://ri.ufs.br/jspui/handle/riufs/15072The aim was to evaluate the effects of soybean oil supplementation associated with the administration of bovine recombinant somatotropin (rBST) on the population of ovarian follicles, standardization of the superovulation response, and embryo recovery. We used 40 Holstein heifers submitted to a 2x2 factorial, with two diets, without or with soybean oil supplementation; and the administration or not of rBST seven days after estrus, forming the experimental groups: DC-SL (control diet plus saline solution application); OL-SL (diet with soybean oil plus saline application); DC-rBST (control diet plus application of 320 mg rBST) e OL-rBST (diet with soybean oil plus the application of 320 mg rBST). The animals received two applications of 500 mg of PGF2α for estrus synchronization and were superovulated with 400 IU of FSH in decreasing daily doses for four consecutive days. On the day before the start of superovulation, the two largest follicles were punctured to quantify the intrafollicular concentration of high-density lipoprotein (HDL). Statistical analyzes were processed using the Action R statistical software version 3.0.2, using ANOVA and Fisher's Test (F test; two comparisons) for the mean characteristics with a probability level of 5%. There were no differences in dry matter intake, follicular activity, P4 concentration, and superovulation response. The group OL-rBST got a greater CL area (4.67±0.67 cm2) and a positive effect was evidenced for HDL concentration in the treatment groups OL-SL (40.86±9.8 mg/dL) and OL-rBST (53.67±10.35 mg/dL). Soybean oil supplementation in the diet associated with the administration of rBST did not provide uniformity in the superovulation response and the embryonic recovery of heifer.Objetivou-se avaliar os efeitos da suplementação com óleo de soja e administração de somatotropina recombinante bovina (rBST) sobre a população de folículos ovarianos, uniformização da resposta superovulatória e recuperação embrionária. Foram utilizadas 40 novilhas da raça holandesa submetidas a um fatorial 2x2, sendo duas dietas, sem (DC = controle) ou com suplementação de óleo de soja (OL) e a administração ou não de 320 mg de rBST, sete dias após o primeiro estro e dos estros subsequentes, constituindo os seguintes grupos experimentais: DC-SL (dieta controle mais a aplicação de solução salina); OL-SL (dieta suplementada com gordura mais a aplicação de solução salina); DC-rBST (dieta controle mais a aplicação de 320 mg de rBST) e OL-rBST (dieta suplementada com gordura mais a aplicação de 320 mg de rBST). Os animais receberam duas aplicações de 500 mg de PGF2α para sincronização do estro e, em seguida, foram superovulados com 400 UI de FSH em doses diárias decrescentes durante quatro dias consecutivos. No dia anterior ao início da superovulação, os dois maiores folículos foram puncionados para quantificação da concentração intrafolicular de lipoproteína de alta densidade (HDL). As análises estatísticas foram processadas pelo software estatístico Action R versão 3.0.2, utilizando a ANOVA e Teste Fisher (Teste F; duas comparações) para as médias das características com nível de probabilidade de 5%. Não foram verificadas diferenças no consumo de matéria seca, atividade folicular ovariana, concentração de P4 e resposta superovulatória. Por outro lado, o grupo OL-rBST obteve maior área de CL (4,67±0,67 cm2 ) e um efeito positivo foi evidenciado para concentração de HDL nos grupos de tratamentos OL-SL (40,86±9,8 mg/dL) e OL-rBST (53,67±10,35 mg/d). A suplementação com óleo de soja na dieta associada à administração de rBST não proporcionou uniformização da resposta superovulatória e na recuperação embrionária de novilhas.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESSão CristóvãoporZootecniaNutrição animalSuplementos dietéticosOvulaçãoSuperovulaçãoLipoproteína de alta densidade (HDL)BovinosCorpo lúteoEmbriãoFator de Crescimento Semelhante à Insulina Tipo I (IGF-I)SuperovulationCattleCorpus luteumHigh-density lipoprotein (HDL)EmbryoInsulin-like growth factor 1 (IGF-1)CIENCIAS AGRARIAS::ZOOTECNIADieta enriquecida com lipídeo associada à somatotropina recombinante bovina em novilhas superovuladasLipid-enriched diet associated with bovine recombinant somatotropin in superovulated heifersinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisPós-Graduação Integrada em ZootecniaUniversidade Federal de Sergipereponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain; charset=utf-81475https://ri.ufs.br/jspui/bitstream/riufs/15072/1/license.txt098cbbf65c2c15e1fb2e49c5d306a44cMD51ORIGINALRICHELLE_MATOS_OLIVEIRA.pdfRICHELLE_MATOS_OLIVEIRA.pdfapplication/pdf512562https://ri.ufs.br/jspui/bitstream/riufs/15072/2/RICHELLE_MATOS_OLIVEIRA.pdf3f9628dd5a09325b8ba8bef12c477724MD52TEXTRICHELLE_MATOS_OLIVEIRA.pdf.txtRICHELLE_MATOS_OLIVEIRA.pdf.txtExtracted texttext/plain66003https://ri.ufs.br/jspui/bitstream/riufs/15072/3/RICHELLE_MATOS_OLIVEIRA.pdf.txt24fc406d334c1bbc3b75e92cfba76ae5MD53THUMBNAILRICHELLE_MATOS_OLIVEIRA.pdf.jpgRICHELLE_MATOS_OLIVEIRA.pdf.jpgGenerated Thumbnailimage/jpeg1584https://ri.ufs.br/jspui/bitstream/riufs/15072/4/RICHELLE_MATOS_OLIVEIRA.pdf.jpg116bba96af8a58d2db2747f4fef4ebc6MD54riufs/150722022-02-23 15:22:25.125oai:ufs.br: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Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2022-02-23T18:22:25Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false
dc.title.pt_BR.fl_str_mv Dieta enriquecida com lipídeo associada à somatotropina recombinante bovina em novilhas superovuladas
dc.title.alternative.eng.fl_str_mv Lipid-enriched diet associated with bovine recombinant somatotropin in superovulated heifers
title Dieta enriquecida com lipídeo associada à somatotropina recombinante bovina em novilhas superovuladas
spellingShingle Dieta enriquecida com lipídeo associada à somatotropina recombinante bovina em novilhas superovuladas
Oliveira, Richelle Matos
Zootecnia
Nutrição animal
Suplementos dietéticos
Ovulação
Superovulação
Lipoproteína de alta densidade (HDL)
Bovinos
Corpo lúteo
Embrião
Fator de Crescimento Semelhante à Insulina Tipo I (IGF-I)
Superovulation
Cattle
Corpus luteum
High-density lipoprotein (HDL)
Embryo
Insulin-like growth factor 1 (IGF-1)
CIENCIAS AGRARIAS::ZOOTECNIA
title_short Dieta enriquecida com lipídeo associada à somatotropina recombinante bovina em novilhas superovuladas
title_full Dieta enriquecida com lipídeo associada à somatotropina recombinante bovina em novilhas superovuladas
title_fullStr Dieta enriquecida com lipídeo associada à somatotropina recombinante bovina em novilhas superovuladas
title_full_unstemmed Dieta enriquecida com lipídeo associada à somatotropina recombinante bovina em novilhas superovuladas
title_sort Dieta enriquecida com lipídeo associada à somatotropina recombinante bovina em novilhas superovuladas
author Oliveira, Richelle Matos
author_facet Oliveira, Richelle Matos
author_role author
dc.contributor.author.fl_str_mv Oliveira, Richelle Matos
dc.contributor.advisor1.fl_str_mv Santos, Anselmo Domingos Ferreira
contributor_str_mv Santos, Anselmo Domingos Ferreira
dc.subject.por.fl_str_mv Zootecnia
Nutrição animal
Suplementos dietéticos
Ovulação
Superovulação
Lipoproteína de alta densidade (HDL)
Bovinos
Corpo lúteo
Embrião
Fator de Crescimento Semelhante à Insulina Tipo I (IGF-I)
Superovulation
Cattle
Corpus luteum
High-density lipoprotein (HDL)
Embryo
Insulin-like growth factor 1 (IGF-1)
topic Zootecnia
Nutrição animal
Suplementos dietéticos
Ovulação
Superovulação
Lipoproteína de alta densidade (HDL)
Bovinos
Corpo lúteo
Embrião
Fator de Crescimento Semelhante à Insulina Tipo I (IGF-I)
Superovulation
Cattle
Corpus luteum
High-density lipoprotein (HDL)
Embryo
Insulin-like growth factor 1 (IGF-1)
CIENCIAS AGRARIAS::ZOOTECNIA
dc.subject.cnpq.fl_str_mv CIENCIAS AGRARIAS::ZOOTECNIA
description The aim was to evaluate the effects of soybean oil supplementation associated with the administration of bovine recombinant somatotropin (rBST) on the population of ovarian follicles, standardization of the superovulation response, and embryo recovery. We used 40 Holstein heifers submitted to a 2x2 factorial, with two diets, without or with soybean oil supplementation; and the administration or not of rBST seven days after estrus, forming the experimental groups: DC-SL (control diet plus saline solution application); OL-SL (diet with soybean oil plus saline application); DC-rBST (control diet plus application of 320 mg rBST) e OL-rBST (diet with soybean oil plus the application of 320 mg rBST). The animals received two applications of 500 mg of PGF2α for estrus synchronization and were superovulated with 400 IU of FSH in decreasing daily doses for four consecutive days. On the day before the start of superovulation, the two largest follicles were punctured to quantify the intrafollicular concentration of high-density lipoprotein (HDL). Statistical analyzes were processed using the Action R statistical software version 3.0.2, using ANOVA and Fisher's Test (F test; two comparisons) for the mean characteristics with a probability level of 5%. There were no differences in dry matter intake, follicular activity, P4 concentration, and superovulation response. The group OL-rBST got a greater CL area (4.67±0.67 cm2) and a positive effect was evidenced for HDL concentration in the treatment groups OL-SL (40.86±9.8 mg/dL) and OL-rBST (53.67±10.35 mg/dL). Soybean oil supplementation in the diet associated with the administration of rBST did not provide uniformity in the superovulation response and the embryonic recovery of heifer.
publishDate 2021
dc.date.issued.fl_str_mv 2021-10-29
dc.date.accessioned.fl_str_mv 2022-02-23T18:18:48Z
dc.date.available.fl_str_mv 2022-02-23T18:18:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv OLIVEIRA, Richelle Matos. Dieta enriquecida com lipídeo associada à somatotropina recombinante bovina em novilhas superovuladas. 2021. 38 f. Dissertação (Mestrado em Zootecnia) – Universidade Federal de Sergipe, São Cristóvão, 2021.
dc.identifier.uri.fl_str_mv https://ri.ufs.br/jspui/handle/riufs/15072
identifier_str_mv OLIVEIRA, Richelle Matos. Dieta enriquecida com lipídeo associada à somatotropina recombinante bovina em novilhas superovuladas. 2021. 38 f. Dissertação (Mestrado em Zootecnia) – Universidade Federal de Sergipe, São Cristóvão, 2021.
url https://ri.ufs.br/jspui/handle/riufs/15072
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.program.fl_str_mv Pós-Graduação Integrada em Zootecnia
dc.publisher.initials.fl_str_mv Universidade Federal de Sergipe
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFS
instname:Universidade Federal de Sergipe (UFS)
instacron:UFS
instname_str Universidade Federal de Sergipe (UFS)
instacron_str UFS
institution UFS
reponame_str Repositório Institucional da UFS
collection Repositório Institucional da UFS
bitstream.url.fl_str_mv https://ri.ufs.br/jspui/bitstream/riufs/15072/1/license.txt
https://ri.ufs.br/jspui/bitstream/riufs/15072/2/RICHELLE_MATOS_OLIVEIRA.pdf
https://ri.ufs.br/jspui/bitstream/riufs/15072/3/RICHELLE_MATOS_OLIVEIRA.pdf.txt
https://ri.ufs.br/jspui/bitstream/riufs/15072/4/RICHELLE_MATOS_OLIVEIRA.pdf.jpg
bitstream.checksum.fl_str_mv 098cbbf65c2c15e1fb2e49c5d306a44c
3f9628dd5a09325b8ba8bef12c477724
24fc406d334c1bbc3b75e92cfba76ae5
116bba96af8a58d2db2747f4fef4ebc6
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)
repository.mail.fl_str_mv repositorio@academico.ufs.br
_version_ 1793351069857742848