Determinação de HPAs presentes na nutrição parenteral e avaliação da exposição de neonatos através da presença do 1-hidroxipireno na urina
Ano de defesa: | 2016 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , , |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Ciências Farmacêuticas
|
Departamento: |
Farmacologia
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/3424 |
Resumo: | Carbon black, which has polycyclic aromatic hydrocarbons (PAHs) adsorbed on its surface, is used in the composition of rubber for pharmaceutical used in injectable drugs and parenteral formulations such as amino acid solutions and lipid emulsions. In this study, the contamination of parenteral preparations administered to newborn infants (NB) by PAHs was evaluated. Commercial solutions used in the composition of parenteral nutrition bags (NP) and the content of the bags administered to a group of ten RN during their period in the neonatal intensive care unit was analyzed. It was also analyzed the metabolite 1-hydroxypyrene in the urine of these infants, as well as in the urine of a group of infants who were not receiving NP (control group). Methods for determination of PAHs and 1-hydroxypyrene by high performance liquid chromatography using detection with DAD and fluorecence, respectively, were developed and validated for the samples of the study. PAHs were extracted from the samples using a polyethylene column. In the urine samples, extraction of metabolite was performed on a C-18 cartridge after its enzymatic hydrolysis. The results showed PAHs contamination in 56 out of 59 samples. Total PAHs concentration ranged from 0.013 mg/L to 10.562 mg/L. Pyrene was present in 50 out of 59 samples. The analysis revealed the presence of the metabolite 1- hydroxypyrene in the urine of babies who received NP, with concentration ranging from 0.039 mg/L to 0.544 mg/L (0.03 and 0.877 mmol/mol creatinine). In the urine of the control group the metabolite was not detected. Considering the toxicity of PAHs and the large volume usually administered in the NP, the results found in this study are worrying. The presence of PAHs in NP administered to newborns is even more important because they are the most vulnerable to adverse effects. |
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2017-01-162017-01-162016-10-19BARICHELLO, Márcia Meister. EVALUATION OF EXPOSURE TO PAHs PRESENT IN PARENTERAL NUTRITION ADMINISTERED TO NEWBORNS THROUGH THE PRESENCE OF 1-HYDROXYPYRENE IN URINE. 2016. 120 f. Tese (Doutorado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2016.http://repositorio.ufsm.br/handle/1/3424Carbon black, which has polycyclic aromatic hydrocarbons (PAHs) adsorbed on its surface, is used in the composition of rubber for pharmaceutical used in injectable drugs and parenteral formulations such as amino acid solutions and lipid emulsions. In this study, the contamination of parenteral preparations administered to newborn infants (NB) by PAHs was evaluated. Commercial solutions used in the composition of parenteral nutrition bags (NP) and the content of the bags administered to a group of ten RN during their period in the neonatal intensive care unit was analyzed. It was also analyzed the metabolite 1-hydroxypyrene in the urine of these infants, as well as in the urine of a group of infants who were not receiving NP (control group). Methods for determination of PAHs and 1-hydroxypyrene by high performance liquid chromatography using detection with DAD and fluorecence, respectively, were developed and validated for the samples of the study. PAHs were extracted from the samples using a polyethylene column. In the urine samples, extraction of metabolite was performed on a C-18 cartridge after its enzymatic hydrolysis. The results showed PAHs contamination in 56 out of 59 samples. Total PAHs concentration ranged from 0.013 mg/L to 10.562 mg/L. Pyrene was present in 50 out of 59 samples. The analysis revealed the presence of the metabolite 1- hydroxypyrene in the urine of babies who received NP, with concentration ranging from 0.039 mg/L to 0.544 mg/L (0.03 and 0.877 mmol/mol creatinine). In the urine of the control group the metabolite was not detected. Considering the toxicity of PAHs and the large volume usually administered in the NP, the results found in this study are worrying. The presence of PAHs in NP administered to newborns is even more important because they are the most vulnerable to adverse effects.O negro de fumo, que possui hidrocarbonetos policíclicos aromáticos (HPAs) adsorvidos em sua superfície, é utilizado na composição de borrachas de uso farmacêutico usadas em medicamentos injetáveis e formulações parenterais como soluções de aminoácidos e emulsões lipídicas. Neste estudo avaliou-se a contaminação por HPAs em preparações parenterais administradas a recémnascidos (RN). Soluções comerciais usadas na composição das bolsas de nutrição parenteral (NP) bem como o conteúdo das próprias bolsas administradas a um grupo de dez RN durante o período de internação na UTI neonatal foram analisados. Foi também analisado o metabólito 1-hidroxipireno na urina desses RN, bem como de um grupo de RN que não estava recebendo NP (grupo controle). Os métodos para determinação dos HPAs e do 1-hidroxipireno por cromatografia líquida de alta eficiência usando detecção por arranjo de fotodiodos e fluorescência, respectivamente, foram desenvolvidos e validados para as amostras do estudo. Os HPAs foram extraídos das amostras utilizando uma coluna de polietileno. Nas amostras de urina, a extração do metabólito foi realizada em um cartucho C-18 após hidrólise enzimática do mesmo. Os resultados mostraram contaminação por HPAs em 56 das 59 amostras analisadas. O total de HPAs variou de 0,013 mg/L a 10,562 mg/L. O pireno esteve presente em 50 das 59 amostras. A análise das urinas revelou a presença do metabólito 1-hidróxipireno para os bebes que receberam NP, com concentração média entre 0,039 μg/L e 0,544 μg/L (0,03 e 0,877 μmol/mol de creatinina). Na urina dos bebes controle o metabólito não foi detectado. Considerando a toxicidade dos HPAs e o grande volume usualmente administrado na NP, os resultados encontrados nesse estudo são preocupantes. A presença de HPAs na NP administrada a recém-nascidos é ainda mais relevante, dado que são os mais vulneráveis a efeitos adversos.application/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Ciências FarmacêuticasUFSMBRFarmacologiaHPAsNutrição parenteral1-hidroxipirenoRecém-nascidoCromatografia líquida de alta eficiênciaValidaçãoPAHsParenteral nutrition1-hydroxypyreneNewbornHPLCValidationCNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIADeterminação de HPAs presentes na nutrição parenteral e avaliação da exposição de neonatos através da presença do 1-hidroxipireno na urinaEvaluation of exposure to PAHs present in parenteral nutrition administered to newborns through the presence of 1-hydroxypyrene in urineinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisNascimento, Denise Bohrer dohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4798557Z7Thiesen, Flavia Valladãohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785907A8Barros, Helena Maria Tannhauserhttp://lattes.cnpq.br/1930793797454956Noremberg, Simone Moraes da Silvahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4221886E0Cardoso, Carmem Dickowhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4792018P1http://lattes.cnpq.br/1693988508491890Barichello, Márcia Meister2010000000004003003003003003003001c5af0ad-15cf-4a7e-9b25-b8b6b1363ae6add5fb29-525c-4636-8e98-ef6c13c15fe6e802cd2b-1aa6-48ae-9d9f-fc415f4cdaa28bd0fb55-5062-4788-9c4b-fdd62a646350a107e148-0a3e-43a8-9b1a-b36c6514ff780acd8ac8-82e1-46cb-9d40-a68c5b505fbbinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALBARICHELLO, MARCIA MEISTER.pdfapplication/pdf3280018http://repositorio.ufsm.br/bitstream/1/3424/1/BARICHELLO%2c%20MARCIA%20MEISTER.pdf6ed0716b709bc081b87d003d255aa5f9MD51TEXTBARICHELLO, MARCIA MEISTER.pdf.txtBARICHELLO, MARCIA MEISTER.pdf.txtExtracted texttext/plain204005http://repositorio.ufsm.br/bitstream/1/3424/2/BARICHELLO%2c%20MARCIA%20MEISTER.pdf.txt047977d6c0e6143832cc3ab82d208debMD52THUMBNAILBARICHELLO, MARCIA MEISTER.pdf.jpgBARICHELLO, MARCIA MEISTER.pdf.jpgIM Thumbnailimage/jpeg4639http://repositorio.ufsm.br/bitstream/1/3424/3/BARICHELLO%2c%20MARCIA%20MEISTER.pdf.jpg61cda5fd6be48d6542f8307eaec93075MD531/34242017-07-30 17:11:40.458oai:repositorio.ufsm.br:1/3424Repositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestopendoar:39132017-07-30T20:11:40Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Determinação de HPAs presentes na nutrição parenteral e avaliação da exposição de neonatos através da presença do 1-hidroxipireno na urina |
dc.title.alternative.eng.fl_str_mv |
Evaluation of exposure to PAHs present in parenteral nutrition administered to newborns through the presence of 1-hydroxypyrene in urine |
title |
Determinação de HPAs presentes na nutrição parenteral e avaliação da exposição de neonatos através da presença do 1-hidroxipireno na urina |
spellingShingle |
Determinação de HPAs presentes na nutrição parenteral e avaliação da exposição de neonatos através da presença do 1-hidroxipireno na urina Barichello, Márcia Meister HPAs Nutrição parenteral 1-hidroxipireno Recém-nascido Cromatografia líquida de alta eficiência Validação PAHs Parenteral nutrition 1-hydroxypyrene Newborn HPLC Validation CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
title_short |
Determinação de HPAs presentes na nutrição parenteral e avaliação da exposição de neonatos através da presença do 1-hidroxipireno na urina |
title_full |
Determinação de HPAs presentes na nutrição parenteral e avaliação da exposição de neonatos através da presença do 1-hidroxipireno na urina |
title_fullStr |
Determinação de HPAs presentes na nutrição parenteral e avaliação da exposição de neonatos através da presença do 1-hidroxipireno na urina |
title_full_unstemmed |
Determinação de HPAs presentes na nutrição parenteral e avaliação da exposição de neonatos através da presença do 1-hidroxipireno na urina |
title_sort |
Determinação de HPAs presentes na nutrição parenteral e avaliação da exposição de neonatos através da presença do 1-hidroxipireno na urina |
author |
Barichello, Márcia Meister |
author_facet |
Barichello, Márcia Meister |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Nascimento, Denise Bohrer do |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4798557Z7 |
dc.contributor.referee1.fl_str_mv |
Thiesen, Flavia Valladão |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785907A8 |
dc.contributor.referee2.fl_str_mv |
Barros, Helena Maria Tannhauser |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/1930793797454956 |
dc.contributor.referee3.fl_str_mv |
Noremberg, Simone Moraes da Silva |
dc.contributor.referee3Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4221886E0 |
dc.contributor.referee4.fl_str_mv |
Cardoso, Carmem Dickow |
dc.contributor.referee4Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4792018P1 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1693988508491890 |
dc.contributor.author.fl_str_mv |
Barichello, Márcia Meister |
contributor_str_mv |
Nascimento, Denise Bohrer do Thiesen, Flavia Valladão Barros, Helena Maria Tannhauser Noremberg, Simone Moraes da Silva Cardoso, Carmem Dickow |
dc.subject.por.fl_str_mv |
HPAs Nutrição parenteral 1-hidroxipireno Recém-nascido Cromatografia líquida de alta eficiência Validação |
topic |
HPAs Nutrição parenteral 1-hidroxipireno Recém-nascido Cromatografia líquida de alta eficiência Validação PAHs Parenteral nutrition 1-hydroxypyrene Newborn HPLC Validation CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
dc.subject.eng.fl_str_mv |
PAHs Parenteral nutrition 1-hydroxypyrene Newborn HPLC Validation |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
description |
Carbon black, which has polycyclic aromatic hydrocarbons (PAHs) adsorbed on its surface, is used in the composition of rubber for pharmaceutical used in injectable drugs and parenteral formulations such as amino acid solutions and lipid emulsions. In this study, the contamination of parenteral preparations administered to newborn infants (NB) by PAHs was evaluated. Commercial solutions used in the composition of parenteral nutrition bags (NP) and the content of the bags administered to a group of ten RN during their period in the neonatal intensive care unit was analyzed. It was also analyzed the metabolite 1-hydroxypyrene in the urine of these infants, as well as in the urine of a group of infants who were not receiving NP (control group). Methods for determination of PAHs and 1-hydroxypyrene by high performance liquid chromatography using detection with DAD and fluorecence, respectively, were developed and validated for the samples of the study. PAHs were extracted from the samples using a polyethylene column. In the urine samples, extraction of metabolite was performed on a C-18 cartridge after its enzymatic hydrolysis. The results showed PAHs contamination in 56 out of 59 samples. Total PAHs concentration ranged from 0.013 mg/L to 10.562 mg/L. Pyrene was present in 50 out of 59 samples. The analysis revealed the presence of the metabolite 1- hydroxypyrene in the urine of babies who received NP, with concentration ranging from 0.039 mg/L to 0.544 mg/L (0.03 and 0.877 mmol/mol creatinine). In the urine of the control group the metabolite was not detected. Considering the toxicity of PAHs and the large volume usually administered in the NP, the results found in this study are worrying. The presence of PAHs in NP administered to newborns is even more important because they are the most vulnerable to adverse effects. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-10-19 |
dc.date.accessioned.fl_str_mv |
2017-01-16 |
dc.date.available.fl_str_mv |
2017-01-16 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BARICHELLO, Márcia Meister. EVALUATION OF EXPOSURE TO PAHs PRESENT IN PARENTERAL NUTRITION ADMINISTERED TO NEWBORNS THROUGH THE PRESENCE OF 1-HYDROXYPYRENE IN URINE. 2016. 120 f. Tese (Doutorado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2016. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/3424 |
identifier_str_mv |
BARICHELLO, Márcia Meister. EVALUATION OF EXPOSURE TO PAHs PRESENT IN PARENTERAL NUTRITION ADMINISTERED TO NEWBORNS THROUGH THE PRESENCE OF 1-HYDROXYPYRENE IN URINE. 2016. 120 f. Tese (Doutorado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2016. |
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http://repositorio.ufsm.br/handle/1/3424 |
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por |
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Programa de Pós-Graduação em Ciências Farmacêuticas |
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UFSM |
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Farmacologia |
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Universidade Federal de Santa Maria |
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