Efeito antiproliferativo, germinação de sementes e diversidade genética de Hesperozygis ringens (Benth.) Epling (Lamiaceae)
Ano de defesa: | 2021 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
Centro de Ciências Naturais e Exatas |
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Agrobiologia
|
Departamento: |
Ciências Biológicas
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/23329 |
Resumo: | Hesperozygis ringens is a species belonging to Lamiaceae. This shrub plant is endemic to the Rio Grande do Sul and is located in restricted areas of a few municipalities in the Rio Grande do Sul. Popularly known as "espanta-pulga", it has antiparasitic, anesthetic and allelopathic effects due to the chemical compounds of essential oils. Currently, the species is threatened with extinction and there are few studies related to its conservation. Studies carried out on the biological effects make a plant promising in elaborating natural products for parasite control. This work aimed to genetically and physiologically characterize Hesperozygis ringens to contribute to the conservation of the species. Samples were collected from the municipalities of Santa Maria, Caçapava do Sul, São Francisco de Assis and São Pedro do Sul. The Allium cepa test was used to verify the genotoxicity of H. ringens. The genetic diversity was analyzed using ISSR molecular markers. The statistical analyzes were performed using Sisvar, GenAlEx Structure and PowerMarker softwares. The results showed that the aqueous extracts of H. ringens has an antiproliferative and non-genotoxic effect on Allium cepa roots. The population presented lower genetic diversity indexes, however, they configure genetically well-structured groups. The seeds of H. ringens had higher germination rates in the presence of light and at a temperature of 15 ºC. The search for better reproduction strategies and the preparation of conservation plans for the species and habitats are fundamental. |
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network_acronym_str |
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network_name_str |
Manancial - Repositório Digital da UFSM |
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spelling |
2021-12-15T18:28:48Z2021-12-15T18:28:48Z2021-08-10http://repositorio.ufsm.br/handle/1/23329Hesperozygis ringens is a species belonging to Lamiaceae. This shrub plant is endemic to the Rio Grande do Sul and is located in restricted areas of a few municipalities in the Rio Grande do Sul. Popularly known as "espanta-pulga", it has antiparasitic, anesthetic and allelopathic effects due to the chemical compounds of essential oils. Currently, the species is threatened with extinction and there are few studies related to its conservation. Studies carried out on the biological effects make a plant promising in elaborating natural products for parasite control. This work aimed to genetically and physiologically characterize Hesperozygis ringens to contribute to the conservation of the species. Samples were collected from the municipalities of Santa Maria, Caçapava do Sul, São Francisco de Assis and São Pedro do Sul. The Allium cepa test was used to verify the genotoxicity of H. ringens. The genetic diversity was analyzed using ISSR molecular markers. The statistical analyzes were performed using Sisvar, GenAlEx Structure and PowerMarker softwares. The results showed that the aqueous extracts of H. ringens has an antiproliferative and non-genotoxic effect on Allium cepa roots. The population presented lower genetic diversity indexes, however, they configure genetically well-structured groups. The seeds of H. ringens had higher germination rates in the presence of light and at a temperature of 15 ºC. The search for better reproduction strategies and the preparation of conservation plans for the species and habitats are fundamental.Hesperozygis ringens é uma espécie pertencente à Lamiaceae. Esta planta arbustiva é endêmica do Rio Grande do Sul e está localizada em áreas restritas de poucos municípios gaúchos. Popularmente conhecida como “espanta-pulga”, possui efeitos antiparasitários, anestésicos e alelopáticos em virtude dos compostos químicos dos óleos essenciais. Atualmente a espécie se encontra ameaçada de extinção e poucos são os estudos relacionados a sua conservação. Trabalhos realizados sobre os efeitos biológicos a torna uma planta promissora na elaboração produtos naturais para controle de parasitas. O objetivo deste trabalho foi caracterizar geneticamente e fisiologicamente Hesperozygis ringens como contribuição à conservação da espécie. Foram coletadas amostras dos municípios de Santa Maria, Caçapava do Sul, São Francisco de Assis e São Pedro do Sul. O teste Allium cepa foi utilizado para verificação da genotoxicidade de H. ringens. A diversidade genética foi analisada através de marcadores moleculares ISSR. As análises estatísticas foram realizadas nos softwares Sisvar, GenAlEx, Structure e PowerMarker. Os resultados apontaram que os extratos aquosos de H. ringens têm efeito antiproliferativo e não genotóxico sobre as raízes de Allium cepa. As populações apresentaram baixos índices de diversidade genética, porém configuram populações bem estruturadas geneticamente. As sementes de H. ringens obtiveram maiores taxas de germinação na presença de luz e na temperatura de 15 ºC. São fundamentais, a busca de melhores estratégias de reprodução e a elaboração de planos de conservação da espécie e dos hábitats.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de Santa MariaCentro de Ciências Naturais e ExatasPrograma de Pós-Graduação em AgrobiologiaUFSMBrasilCiências BiológicasAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessConservaçãoEspécie ameaçadaBioma PampaÓleos essenciaisGenotoxicidadeConservationEndangered speciesPampa biomeEssential oilsGenotoxicityCNPQ::CIENCIAS BIOLOGICASEfeito antiproliferativo, germinação de sementes e diversidade genética de Hesperozygis ringens (Benth.) Epling (Lamiaceae)Antiproliferative effect, seed germination and genetic diversity of Hesperozygis ringens (Benth.) 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dc.title.por.fl_str_mv |
Efeito antiproliferativo, germinação de sementes e diversidade genética de Hesperozygis ringens (Benth.) Epling (Lamiaceae) |
dc.title.alternative.eng.fl_str_mv |
Antiproliferative effect, seed germination and genetic diversity of Hesperozygis ringens (Benth.) Epling (Lamiaceae) |
title |
Efeito antiproliferativo, germinação de sementes e diversidade genética de Hesperozygis ringens (Benth.) Epling (Lamiaceae) |
spellingShingle |
Efeito antiproliferativo, germinação de sementes e diversidade genética de Hesperozygis ringens (Benth.) Epling (Lamiaceae) Leite, Leandro Gonçalves Conservação Espécie ameaçada Bioma Pampa Óleos essenciais Genotoxicidade Conservation Endangered species Pampa biome Essential oils Genotoxicity CNPQ::CIENCIAS BIOLOGICAS |
title_short |
Efeito antiproliferativo, germinação de sementes e diversidade genética de Hesperozygis ringens (Benth.) Epling (Lamiaceae) |
title_full |
Efeito antiproliferativo, germinação de sementes e diversidade genética de Hesperozygis ringens (Benth.) Epling (Lamiaceae) |
title_fullStr |
Efeito antiproliferativo, germinação de sementes e diversidade genética de Hesperozygis ringens (Benth.) Epling (Lamiaceae) |
title_full_unstemmed |
Efeito antiproliferativo, germinação de sementes e diversidade genética de Hesperozygis ringens (Benth.) Epling (Lamiaceae) |
title_sort |
Efeito antiproliferativo, germinação de sementes e diversidade genética de Hesperozygis ringens (Benth.) Epling (Lamiaceae) |
author |
Leite, Leandro Gonçalves |
author_facet |
Leite, Leandro Gonçalves |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Essi, Liliana |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/5776430118696894 |
dc.contributor.advisor-co1.fl_str_mv |
Mazzocato, Ana Cristina |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/9244427070711936 |
dc.contributor.referee1.fl_str_mv |
Silva, Antonio Carlos Ferreira da |
dc.contributor.referee2.fl_str_mv |
Ferreira, Juliano Lino |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/6248702051833568 |
dc.contributor.author.fl_str_mv |
Leite, Leandro Gonçalves |
contributor_str_mv |
Essi, Liliana Mazzocato, Ana Cristina Silva, Antonio Carlos Ferreira da Ferreira, Juliano Lino |
dc.subject.por.fl_str_mv |
Conservação Espécie ameaçada Bioma Pampa Óleos essenciais Genotoxicidade |
topic |
Conservação Espécie ameaçada Bioma Pampa Óleos essenciais Genotoxicidade Conservation Endangered species Pampa biome Essential oils Genotoxicity CNPQ::CIENCIAS BIOLOGICAS |
dc.subject.eng.fl_str_mv |
Conservation Endangered species Pampa biome Essential oils Genotoxicity |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS |
description |
Hesperozygis ringens is a species belonging to Lamiaceae. This shrub plant is endemic to the Rio Grande do Sul and is located in restricted areas of a few municipalities in the Rio Grande do Sul. Popularly known as "espanta-pulga", it has antiparasitic, anesthetic and allelopathic effects due to the chemical compounds of essential oils. Currently, the species is threatened with extinction and there are few studies related to its conservation. Studies carried out on the biological effects make a plant promising in elaborating natural products for parasite control. This work aimed to genetically and physiologically characterize Hesperozygis ringens to contribute to the conservation of the species. Samples were collected from the municipalities of Santa Maria, Caçapava do Sul, São Francisco de Assis and São Pedro do Sul. The Allium cepa test was used to verify the genotoxicity of H. ringens. The genetic diversity was analyzed using ISSR molecular markers. The statistical analyzes were performed using Sisvar, GenAlEx Structure and PowerMarker softwares. The results showed that the aqueous extracts of H. ringens has an antiproliferative and non-genotoxic effect on Allium cepa roots. The population presented lower genetic diversity indexes, however, they configure genetically well-structured groups. The seeds of H. ringens had higher germination rates in the presence of light and at a temperature of 15 ºC. The search for better reproduction strategies and the preparation of conservation plans for the species and habitats are fundamental. |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-12-15T18:28:48Z |
dc.date.available.fl_str_mv |
2021-12-15T18:28:48Z |
dc.date.issued.fl_str_mv |
2021-08-10 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/23329 |
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http://repositorio.ufsm.br/handle/1/23329 |
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por |
language |
por |
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200000000006 |
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600 600 600 600 600 600 |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Agrobiologia |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Ciências Biológicas |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
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